>VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCAXXXXXXXXXXAATGTCATCAGTGGT
AGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAA
AGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGT
TTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGAT
CTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGT
GATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGAT
CAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGT
GATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGT
AATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCT
CTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAA
GATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCT
CCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTA
GGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATAT
GNAAACAAAAC

LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILP---

---NVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASE
FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTA
SVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMV
SHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFR
KF*fys*yxnk

Frame A:
tyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhq
vhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismv
pqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqv
in*lcknl*fy---

---NVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASE
FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTA
SVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMV
SHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFR
KF*fys*yxnk

Frame B:
litlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssir
cinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwy
pk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr*
*isyariydft---

---mssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlpln
sklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*qh
qlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswl
aivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitmlv*lsv
nfnfthnmxtk

Frame C:
LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILP---

---chqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl*i
qs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*insi
s*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*
psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lcwfnfp*
ililliixkqn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       2529   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1473   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1473   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1473   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       1473   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1471   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1465   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1459   0.0
SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       1459   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1457   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X80474|X80474.1 N.frontalis enolase gene.                         64  1e-76  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          86  7e-73  11
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  6e-61  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  6e-61  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76  6e-61  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76  6e-59  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92  2e-52  5
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  1e-48  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66  2e-46  6
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.         50  9e-46  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   479   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   352   e-160
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   345   e-157
FJ979826_1(FJ979826|pid:none) Nicotiana tabacum cultivar Samsun ...   347   e-155
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   337   e-154
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   342   e-154
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   347   e-154
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   330   e-154
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   330   e-154
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   349   e-153

 psg: 0.95  gvh: 0.47  alm: 0.32  top: 0.60  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: cytoskeletal

>> prediction for VFE233 is cyt

>VFE233F.Seq
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCA----------

>VFE233Z.Seq
----------AATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGA
AGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGC
CATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGA
ATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGC
TGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAAT
CATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGC
ATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAA
GACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTC
AGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGT
TAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGG
TACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCA
ACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCG
TAAATTTTAATTTTACTCATAATATGNAAACAAAAC

>VFE233P.Seq
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCA----------AATGTCATCAGTGGT
AGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAA
AGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGT
TTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGAT
CTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGT
GATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGAT
CAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGT
GATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGT
AATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCT
CTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAA
GATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCT
CCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTA
GGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATAT
GNAAACAAAAC