>VFE149 (VFE149Q) /CSM/VF/VFE1-C/VFE149Q.Seq.d/
XXXXXXXXXXGAGAATGTAAATTTAACTCTGCCCAAGTTGCTGCTAAAATTTCATCTTTC
ACTATGGTTCCACAAAATGAAACTCAAATTGCTTCCTACTTATTCAACAATGGTCCATTA
GCTATNGCAGCTGATGCTGAAGAATGGCAATTCTATATGGGAGGTGTTTTCGATTTCCCA
TGTGGTCAAACTTTAGATCACGGTATCTTAATTGTTGGTTATGGTGCTCAAGATACCATC
GTCGGTAAAAATACTCCATACTGGATCATTAAAAACTCATGGGGTGCCGNTTGGGGTGAA
GCTGGTTACTTAAAAGTTGAAAGAAATACTGATAAATGTGGTGTTGCCAATTTCGTTTCT

---ECKFNSAQVAAKISSFTMVPQNETQIASYLFNNGPLAXAADAEEWQFYMGGVFDFPC
GQTLDHGILIVGYGAQDTIVGKNTPYWIIKNSWGAXWGEAGYLKVERNTDKCGVANFVS

Frame A:
---rm*i*lcpscc*nfifhygstk*nsncflliqqwsisyxs*c*rmailygrcfrfpm
wsnfrsrylncwlwcsryhrr*kysildh*klmgcrlg*swllks*kky**mwccqfrf


Frame B:
---ECKFNSAQVAAKISSFTMVPQNETQIASYLFNNGPLAXAADAEEWQFYMGGVFDFPC
GQTLDHGILIVGYGAQDTIVGKNTPYWIIKNSWGAXWGEAGYLKVERNTDKCGVANFVS


Frame C:
---nvnltlpklllkfhlslwfhkmklkllptystmvh*lxqlmlkngnsiwevfsishv
vkl*itvs*llvmvlkipssvkilhtgslkthgvpxgvklvt*klkeilinvvlpisf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE149 (VFE149Q) /CSM/VF/VFE1-C/VFE149Q.Seq.d/                        682   0.0
VSB747 (VSB747Q) /CSM/VS/VSB7-B/VSB747Q.Seq.d/                        674   0.0
VFO892 (VFO892Q) /CSM/VF/VFO8-D/VFO892Q.Seq.d/                        674   0.0
VFO806 (VFO806Q) /CSM/VF/VFO8-A/VFO806Q.Seq.d/                        674   0.0
VFO638 (VFO638Q) /CSM/VF/VFO6-B/VFO638Q.Seq.d/                        674   0.0
VFO290 (VFO290Q) /CSM/VF/VFO2-D/VFO290Q.Seq.d/                        674   0.0
VFO273 (VFO273Q) /CSM/VF/VFO2-D/VFO273Q.Seq.d/                        674   0.0
VFN663 (VFN663Q) /CSM/VF/VFN6-C/VFN663Q.Seq.d/                        674   0.0
VFN261 (VFN261Q) /CSM/VF/VFN2-C/VFN261Q.Seq.d/                        674   0.0
VFN103 (VFN103Q) /CSM/VF/VFN1-A/VFN103Q.Seq.d/                        674   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.    46  4e-04  2
L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.    46  5e-04  3
BG525215|BG525215.1 45-7 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    54  0.001  1
BG524692|BG524692.1 6-13 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    54  0.001  1
BG524924|BG524924.1 1-26 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    54  0.001  1
BG524136|BG524136.1 39-26 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    54  0.001  1
BG523152|BG523152.1 29-47 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    54  0.001  1
BE610915|BE610915.1 sq68c07.y1 Gm-c1048 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1048-613 5' similar to TR:Q96454 Q96454 THIOL PROTEASE ISOFORM B ;, mRNA sequence.    44  0.018  2
BQ514757|BQ514757.1 EST622172 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMIN86 3' end, mRNA sequence.    42  0.041  2
BQ514756|BQ514756.1 EST622171 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMIN86 5' end, mRNA sequence.    42  0.041  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04988) RecName: Full=Cysteine proteinase 1;          EC=3.4.22...   171   1e-41
FJ609256_1(FJ609256|pid:none) Solanum lycopersicum cysteine prot...   120   1e-26
FJ475061_1(FJ475061|pid:none) Arachis hypogaea cysteine protease...   118   6e-26
AB270920_1(AB270920|pid:none) Phaseolus vulgaris CP2 gene for cy...   118   7e-26
AJ580823_3(AJ580823|pid:none) Lotus corniculatus var. japonicus ...   117   1e-25
FB844636_1(FB844636|pid:none) Sequence 63909 from Patent WO20080...   117   2e-25
AJ580823_4(AJ580823|pid:none) Lotus corniculatus var. japonicus ...   116   2e-25
U42758_1(U42758|pid:none) Naegleria fowleri cysteine proteinase ...   116   2e-25
AY662991_1(AY662991|pid:none) Petunia x hybrida cysteine protein...   116   2e-25
AC149637_11(AC149637|pid:none) Medicago truncatula clone mth2-18...   115   4e-25

 psg: 0.78  gvh: 0.49  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFE149 is cyt

>VFE149Z.Seq
----------GAGAATGTAAATTTAACTCTGCCCAAGTTGCTGCTAAAATTTCATCTTTC
ACTATGGTTCCACAAAATGAAACTCAAATTGCTTCCTACTTATTCAACAATGGTCCATTA
GCTATNGCAGCTGATGCTGAAGAATGGCAATTCTATATGGGAGGTGTTTTCGATTTCCCA
TGTGGTCAAACTTTAGATCACGGTATCTTAATTGTTGGTTATGGTGCTCAAGATACCATC
GTCGGTAAAAATACTCCATACTGGATCATTAAAAACTCATGGGGTGCCGNTTGGGGTGAA
GCTGGTTACTTAAAAGTTGAAAGAAATACTGATAAATGTGGTGTTGCCAATTTCGTTTCT