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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE127
Library VF
(Link to library)
Clone ID VFE127
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFE127P
(Link to Original site)
Representative DNA sequence 
>VFE127 (VFE127Q) /CSM/VF/VFE1-B/VFE127Q.Seq.d/
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTXXXXXXXXXXCTCTCATTCGGTTCAAT
CGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTG
GGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTAC
TTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTAC
TGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTG
TAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTT
AACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGG
ATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTC
TGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCA
AGGTTAAATTTTTTTAATTAATTTATATTTAAGATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVT---

---LSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSL
IAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTE
FGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr
Translated Amino Acid sequence (All Frames) 
Frame A:
nchltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwy
hnpnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrc
lkhicrqrn*fhhpir*wcyvrfclsrfrhc---

---LSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSL
IAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTE
FGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr

Frame B:
ivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVT---

---shsvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhs
llvqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf*lqkntf*klls
serlnv*vdlwvss*tweisgslvmfsslltilysilvinklvsqlpfkvkff*liyi*d

Frame C:
lspdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvp
qsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavp
qahmsptelispsntvvvlcqvlslkipsl---

---lirfnr*hqihw*hylrpinqrnllgiryg*lcyrwsiswflwyylsrnlrfryith
cwsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icfnskrirfrsy*v
rkd*mfewiygyrvkhgkfldpw*cfhlcllycirfw**tswfrnchsrlnffn*fifki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF355 (VSF355Q) /CSM/VS/VSF3-C/VSF355Q.Seq.d/                       1015   0.0
VSE707 (VSE707Q) /CSM/VS/VSE7-A/VSE707Q.Seq.d/                       1015   0.0
VSC420 (VSC420Q) /CSM/VS/VSC4-A/VSC420Q.Seq.d/                       1015   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1015   0.0
VFM401 (VFM401Q) /CSM/VF/VFM4-A/VFM401Q.Seq.d/                       1015   0.0
VFM234 (VFM234Q) /CSM/VF/VFM2-B/VFM234Q.Seq.d/                       1015   0.0
VFM222 (VFM222Q) /CSM/VF/VFM2-A/VFM222Q.Seq.d/                       1015   0.0
VFM215 (VFM215Q) /CSM/VF/VFM2-A/VFM215Q.Seq.d/                       1015   0.0
VFL883 (VFL883Q) /CSM/VF/VFL8-D/VFL883Q.Seq.d/                       1015   0.0
VFL828 (VFL828Q) /CSM/VF/VFL8-B/VFL828Q.Seq.d/                       1015   0.0
own update 2004. 7.30
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.  1001  0.0  3
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1001  0.0  4
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62  1e-05  1
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62  1e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  1e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62  1e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  1e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  1e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62  1e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  1e-05  1
dna update 2003. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   229   1e-58
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   217   4e-55
AF420068_1(AF420068|pid:none) Clonorchis sinensis aspartic prote...   212   1e-53
DQ131585_1(DQ131585|pid:none) Opisthorchis viverrini cathepsin D...   212   1e-53
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   209   2e-52
EF193385_1(EF193385|pid:none) Musca domestica aspartic proteinas...   201   4e-50
AB458309_1(AB458309|pid:none) Pongo abelii pg mRNA for pepsinoge...   177   4e-43
AB458308_1(AB458308|pid:none) Pongo abelii pg mRNA for pepsinoge...   176   8e-43
AB458311_1(AB458311|pid:none) Pongo abelii pg mRNA for pepsinoge...   176   1e-42
AB458312_1(AB458312|pid:none) Pongo abelii pg mRNA for pepsinoge...   174   3e-42
protein update 2009. 6.18
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.42  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFE127 is exc
5' end seq. ID VFE127F
5' end seq. 
>VFE127F.Seq
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGT----------
Length of 5' end seq. 453
3' end seq. ID VFE127Z
3' end seq. 
>VFE127Z.Seq
----------CTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTA
CGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGG
TCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACT
CATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTT
AAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCAT
CACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGA
GTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTT
CTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAA
ACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAG
ATA
Length of 3' end seq. 533
Connected seq. ID VFE127P
Connected seq. 
>VFE127P.Seq
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGT----------CTCTCATTCGGTTCAAT
CGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTG
GGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTAC
TTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTAC
TGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTG
TAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTT
AACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGG
ATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTC
TGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCA
AGGTTAAATTTTTTTAATTAATTTATATTTAAGATA
Length of connected seq. 986
Full length Seq ID -
Full length Seq. -
Length of full length seq. -