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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD854
Library VF
(Link to library)
Clone ID VFD854
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16272-1
Original site URL
Representative seq. ID VFD854P
(Link to Original site)
Representative DNA sequence 
>VFD854 (VFD854Q) /CSM/VF/VFD8-C/VFD854Q.Seq.d/
TCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCACGGTAATCT
CGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTCATTCCCAAA
GGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGCTGGTATGAC
TCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGAAATCGTTGA
AGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGGTTATGTTGA
AACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAGTGATAACTT
CCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTTCACCAAATA
CGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTCTCCAAGCCAT
CAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACAAATTAAAATT
XXXXXXXXXXTCATTGCTCACACTCAAGTTCACAAATTAAAATTAACTCAAAAGAAAGCA
CACGTCCTTGAAATCCAAGTCAATGGTGGTTCAATCGTCGAAAAAGTTAACTTTGCTGTT
GCCAACTTTGAAAAGACCGTCAACGTCACTGGTGTCTTTGCCGAAAATGAATTAATCGAT
GTTATCGGTGTAACCAAAGGTAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGT
AAATTACCAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCAT
CCATCCCGTGTTAGTACCACCGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTC
GAAAGAAACAAGAAGATTTACAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATC
TCAACCGGTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTC
GCTCACTACGGTATGGTTAAACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCA
AGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTC
GAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAA
ACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAA
ATTCAATAAATTAGATTTCAGAGAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKAGMT
HVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLSDNF
RRRLYKNWYKSKSKKAFTKYVKQYETEEGKKSIEASLQAIKKRCSVVRVIAHTQVHKLK-
--

---IAHTQVHKLKLTQKKAHVLEIQVNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDV
IGVTKGKGFNGVIKRWGVRKLPRKTHKGLRKVACIGAWHPSRVSTTVPRAGQLGYHHRVE
RNKKIYRIGQAQPEDGKQISTGKTEFDLTEKTINPMGGFAHYGMVKHEFLMLKGCVAGPR
KRALTLRKSITTQTGRAALEKITLKFIDTSSKFGHGLHQTAEDKTKYFGVKKSRSTKA*i
q*irfqr
Translated Amino Acid sequence (All Frames) 
Frame A:
skqq*qyess*i*sstsr*srfqtkkesrqtsr*sqvipkg*pypksplncihglqswyd
srcqrfrktrfkdaqkrnr*srhhh*mstnvhcwfswlc*nrsrsqnlqnrlgstfk**l
p*kiiqklvqikikesfhqir*tirn*rr*kin*slspshqkemfsrpchcshsssqiki
---

---slltlkftn*n*lkrkhtslksksmvvqsskkltlllptlkrpstslvslpkmn*sm
lsv*pkvkvsmvslkdgvsvnyqerpikv*erlpvlvhgihpvlvppsqelvnsvtiivs
ketrrftesvklnqktvnksqpvrlnsi*lkrlsiqwvvslttvwlnmns*clkvvllvq
erep*p*ervsplklvvlpsrkslsnssippaslvtvfikplkirlnisasrnqdqlrlk
fnkldfre

Frame B:
LNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKAGMT
HVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLSDNF
RRRLYKNWYKSKSKKAFTKYVKQYETEEGKKSIEASLQAIKKRCSVVRVIAHTQVHKLK-
--

---hcshsssqikinskestrp*npsqwwfnrrks*lcccql*kdrqrhwclcrk*inrc
yrcnqr*rfqwch*kmgcp*itkkdp*rfkkgclywcmasipc*yhrpkswstrlpsscr
kkqedlqnrssstrrr*tnlnr*d*irfn*kdyqsngwfrslryg*t*ilna*rlccwsk
keslnlkkeyhhsnwsccprenhsqihrylqqvwsrsssnr*r*d*ifrrqeikin*gln
sin*ise

Frame C:
*ttiti*vivnlklhvtvisvsdqereppdikvksshsqrmtvpkkst*lhswvtklv*l
tlsei*knqvqrctkkkslkpspslnvhqctllv*lvmlkplkvskptkpsglni*vits
vedytktgtnqnqrklspntlnntklkkvknqlkplskpskrdvqssvslltlkftn*n-
--

---IAHTQVHKLKLTQKKAHVLEIQVNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDV
IGVTKGKGFNGVIKRWGVRKLPRKTHKGLRKVACIGAWHPSRVSTTVPRAGQLGYHHRVE
RNKKIYRIGQAQPEDGKQISTGKTEFDLTEKTINPMGGFAHYGMVKHEFLMLKGCVAGPR
KRALTLRKSITTQTGRAALEKITLKFIDTSSKFGHGLHQTAEDKTKYFGVKKSRSTKA*i
q*irfqr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD854 (VFD854Q) /CSM/VF/VFD8-C/VFD854Q.Seq.d/                       2488   0.0
VFH886 (VFH886Q) /CSM/VF/VFH8-D/VFH886Q.Seq.d/                       1457   0.0
VFF417 (VFF417Q) /CSM/VF/VFF4-A/VFF417Q.Seq.d/                       1457   0.0
VFC883 (VFC883Q) /CSM/VF/VFC8-D/VFC883Q.Seq.d/                       1457   0.0
VFC683 (VFC683Q) /CSM/VF/VFC6-D/VFC683Q.Seq.d/                       1457   0.0
SFH704 (SFH704Q) /CSM/SF/SFH7-A/SFH704Q.Seq.d/                       1453   0.0
VFJ264 (VFJ264Q) /CSM/VF/VFJ2-C/VFJ264Q.Seq.d/                       1451   0.0
VFF634 (VFF634Q) /CSM/VF/VFF6-B/VFF634Q.Seq.d/                       1449   0.0
CFF138 (CFF138Q) /CSM/CF/CFF1-B/CFF138Q.Seq.d/                       1445   0.0
VFI188 (VFI188Q) /CSM/VF/VFI1-D/VFI188Q.Seq.d/                       1443   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L08391|L08391.1 Dictyostelium discoideum ribosomal protein (L3) gene, complete cds.  1027  0.0  3
AX489150|AX489150.1 Sequence 6450 from Patent WO02053728.         78  2e-47  10
Z74971|Z74971.1 S.cerevisiae chromosome XV reading frame ORF YOR063w.    70  6e-35  8
J01351|J01351.1 S.cerevisiae ribosomal protein L3 (tcm1) gene.    62  3e-34  8
Z70678|Z70678.1 S.cerevisiae chromosome XV DNA, 54.7 kb region.    70  7e-27  9
BE641342|BE641342.1 Cri2_3_A04_SP6 Ceratopteris Spore Library Ceratopteris richardii cDNA clone Cri2_3_A04 5', mRNA sequence.    76  1e-21  3
AL424946|AL424946.1 clone BA0AB002D01 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    66  3e-19  5
AX765804|AX765804.1 Sequence 13 from Patent WO03008440.           74  4e-19  4
AY052743|AY052743.1 Arabidopsis thaliana At1g43170/F1I21_18 mRNA, complete cds.    74  4e-19  4
AF361101|AF361101.1 Arabidopsis thaliana putative L3 cytoplasmic ribosomal protein (F1I21.L3) mRNA, complete cds.    74  4e-19  4
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC158279_1(BC158279|pid:none) Xenopus tropicalis hypothetical pr...   305   e-150
AJ720292_1(AJ720292|pid:none) Gallus gallus mRNA for hypothetica...   300   e-148
(P39023) RecName: Full=60S ribosomal protein L3; AltName: Full=H...   295   e-148
AK151142_1(AK151142|pid:none) Mus musculus bone marrow macrophag...   295   e-147
(P39872) RecName: Full=60S ribosomal protein L3;  &BC102253_1(BC...   295   e-147
AK151057_1(AK151057|pid:none) Mus musculus bone marrow macrophag...   295   e-147
(P21531) RecName: Full=60S ribosomal protein L3; AltName: Full=L...   295   e-147
BC008492_1(BC008492|pid:none) Homo sapiens ribosomal protein L3,...   293   e-147
AJ238851_1(AJ238851|pid:none) Bos taurus rpl3 gene for ribosomal...   294   e-147
BC151555_1(BC151555|pid:none) Bos taurus ribosomal protein L3, m...   295   e-147
protein update 2009. 6.18
PSORT 

 psg: 0.52  gvh: 0.21  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.06
 nuc: 0.39  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VFD854 is cyt
5' end seq. ID VFD854F
5' end seq. 
>VFD854F.Seq
TCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCACGGTAATCT
CGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTCATTCCCAAA
GGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGCTGGTATGAC
TCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGAAATCGTTGA
AGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGGTTATGTTGA
AACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAGTGATAACTT
CCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTTCACCAAATA
CGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTCTCCAAGCCAT
CAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACAAATTAAAATT
----------
Length of 5' end seq. 540
3' end seq. ID VFD854Z
3' end seq. 
>VFD854Z.Seq
----------TCATTGCTCACACTCAAGTTCACAAATTAAAATTAACTCAAAAGAAAGCA
CACGTCCTTGAAATCCAAGTCAATGGTGGTTCAATCGTCGAAAAAGTTAACTTTGCTGTT
GCCAACTTTGAAAAGACCGTCAACGTCACTGGTGTCTTTGCCGAAAATGAATTAATCGAT
GTTATCGGTGTAACCAAAGGTAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGT
AAATTACCAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCAT
CCATCCCGTGTTAGTACCACCGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTC
GAAAGAAACAAGAAGATTTACAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATC
TCAACCGGTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTC
GCTCACTACGGTATGGTTAAACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCA
AGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTC
GAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAA
ACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAA
ATTCAATAAATTAGATTTCAGAGAG
Length of 3' end seq. 735
Connected seq. ID VFD854P
Connected seq. 
>VFD854P.Seq
TCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCACGGTAATCT
CGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTCATTCCCAAA
GGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGCTGGTATGAC
TCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGAAATCGTTGA
AGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGGTTATGTTGA
AACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAGTGATAACTT
CCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTTCACCAAATA
CGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTCTCCAAGCCAT
CAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACAAATTAAAATT
----------TCATTGCTCACACTCAAGTTCACAAATTAAAATTAACTCAAAAGAAAGCA
CACGTCCTTGAAATCCAAGTCAATGGTGGTTCAATCGTCGAAAAAGTTAACTTTGCTGTT
GCCAACTTTGAAAAGACCGTCAACGTCACTGGTGTCTTTGCCGAAAATGAATTAATCGAT
GTTATCGGTGTAACCAAAGGTAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGT
AAATTACCAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCAT
CCATCCCGTGTTAGTACCACCGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTC
GAAAGAAACAAGAAGATTTACAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATC
TCAACCGGTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTC
GCTCACTACGGTATGGTTAAACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCA
AGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTC
GAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAA
ACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAA
ATTCAATAAATTAGATTTCAGAGAG
Length of connected seq. 1275
Full length Seq ID -
Full length Seq. -
Length of full length seq. -