>VFD730 (VFD730Q) /CSM/VF/VFD7-B/VFD730Q.Seq.d/
XXXXXXXXXXACCACCACCACCACCTCCACCACCACCTTCTAGTGGTCCACCACCTCCAC
CACCACCAATGGCCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCGGTGGAA
GAAATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATAAAACCA
ATCCACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTGATACTT
TAGCTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAGATGATG
ATGACGATGATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATTCAAAAA
AATCATTAAATAAAGATTT

---PPPPPPPPPSSGPPPPPPPMASAPPSSGGGGASGGGRNDLLASIRSGASLKAVDKTN
PLPDIQSLGNEGSRSLADTLAAAMANRRGGMREDDEEDDDDDDEWSDY*iil*qnsnskk
slnkd

Frame A:
---PPPPPPPPPSSGPPPPPPPMASAPPSSGGGGASGGGRNDLLASIRSGASLKAVDKTN
PLPDIQSLGNEGSRSLADTLAAAMANRRGGMREDDEEDDDDDDEWSDY*iil*qnsnskk
slnkd


Frame B:
---hhhhlhhhllvvhhlhhhqwpvllhhqevvvpaaveemifwlpfvvvqvlrllikpi
hsqifkvlvmkvhvl*lil*lqlwlivevv*ekmmkkmmmtmmngqiiklffnkiqiqkn
h*iki


Frame C:
---ttttstttf*wsttstttngqcssiirrwwcqrrwkk*sfgfhs*wcks*gc**nqs
tprysksw**rftffs*yfscsyg*s*rwyerr**rr***r**mvrllnysltkfkfkki
ik*rf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN644 (VFN644Q) /CSM/VF/VFN6-B/VFN644Q.Seq.d/                        410   e-114
VFD730 (VFD730Q) /CSM/VF/VFD7-B/VFD730Q.Seq.d/                        410   e-114
VFA863 (VFA863Q) /CSM/VF/VFA8-C/VFA863Q.Seq.d/                        410   e-114
SSG705 (SSG705Q) /CSM/SS/SSG7-A/SSG705Q.Seq.d/                        410   e-114
SLH341 (SLH341Q) /CSM/SL/SLH3-B/SLH341Q.Seq.d/                        410   e-114
SLH135 (SLH135Q) /CSM/SL/SLH1-B/SLH135Q.Seq.d/                        410   e-114
SHE721 (SHE721Q) /CSM/SH/SHE7-A/SHE721Q.Seq.d/                        410   e-114
SFK712 (SFK712Q) /CSM/SF/SFK7-A/SFK712Q.Seq.d/                        410   e-114
SFJ736 (SFJ736Q) /CSM/SF/SFJ7-B/SFJ736Q.Seq.d/                        410   e-114
SFF823 (SFF823Q) /CSM/SF/SFF8-A/SFF823Q.Seq.d/                        410   e-114

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF305060|AF305060.1 Dictyostelium discoideum Wiscott-Aldrich syndrome protein (wasA) gene, complete cds.   410  e-129  3
BQ258443|BQ258443.1 fz79g06.y1 Sugano SJD adult male Danio rerio cDNA clone 5914930 5' similar to SW:ROA1_HUMAN P09651 HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 ;, mRNA sequence.    50  0.020  1
AY088091|AY088091.1 Arabidopsis thaliana clone 41181 mRNA, complete sequence.    46  0.14  2
X75304|X75304.1 H.sapiens giantin mRNA.                           44  0.53  2
Z99161|Z99161.1 S.pombe chromosome I cosmid c11G7.                44  0.78  2
AC024907|AC024907.29 Homo sapiens chromosome 3 clone RP11-911E23, WORKING DRAFT SEQUENCE, 42 unordered pieces.    44  1.2  1
BM546955|BM546955.1 AGENCOURT_6491265 NIH_MGC_125 Homo sapiens cDNA clone IMAGE:5723733 5', mRNA sequence.    44  1.2  1
BM466550|BM466550.1 AGENCOURT_6431710 NIH_MGC_67 Homo sapiens cDNA clone IMAGE:5503648 5', mRNA sequence.    44  1.2  1
CF121974|CF121974.1 UI-HF-BP0p-art-f-03-0-UI.r1 NIH_MGC_51 Homo sapiens cDNA clone IMAGE:30567422 5', mRNA sequence.    44  1.2  1
D25542|D25542.1 Human mRNA for golgi antigen gcp372, complete cds.    44  1.2  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF305060_1(AF305060|pid:none) Dictyostelium discoideum Wiscott-A...    83   3e-15
AC117076_18(AC117076|pid:none) Dictyostelium discoideum chromoso...    39   0.043
DQ985464_1(DQ985464|pid:none) Suberites domuncula Wiskott-Aldric...    32   6.8

 psg: 0.84  gvh: 0.43  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFD730 is cyt

>VFD730Z.Seq
----------ACCACCACCACCACCTCCACCACCACCTTCTAGTGGTCCACCACCTCCAC
CACCACCAATGGCCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCGGTGGAA
GAAATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATAAAACCA
ATCCACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTGATACTT
TAGCTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAGATGATG
ATGACGATGATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATTCAAAAA
AATCATTAAATAAAGATTT