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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD589
Library VF
(Link to library)
Clone ID VFD589
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16173-1
Original site URL
Representative seq. ID VFD589P
(Link to Original site)
Representative DNA sequence 
>VFD589 (VFD589Q) /CSM/VF/VFD5-D/VFD589Q.Seq.d/
AGTCTCCACCAAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAAAATT
TTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGTAATG
AAGTTAGGGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCAT
TTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCA
ATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAG
TACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTT
TAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAATAG
TTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACAT
TAGCAGTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAA
TGTCATCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAA
TCACTCGTGTGAAAACCXXXXXXXXXXCGTATCGCCAAATCATGTGGTGGTACAGTCTTA
ATCACACTTGCAAATCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGTATTGCC
GATGAAGTTGTTCAAGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAACTCAAAT
AAGAAATCAGCTTCAATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAA
CGTTCCATTCATGACTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTT
CCAGGTGGTGGTGCAGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCCGCCACA
ATGGGTAGTCGTAAACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCA
AAACAATTGGCTGTAAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCC
TATCATCATGCCGCTCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGAT
TTATTCAATAACAAAGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCC
AAAATCAAATGTATCAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATCGATGAT
AAAATCACTCTTAATCCAAGAGAACAACAAGG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRISGNEVRAQNVLAVTAIANIVKTSF
GPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDGTTTVVIL
AAELLKRANELVARKVHPIVIISGFRLACTEAIKYINETLAVKVETLPKDFIVNIAKTSM
SSKTINDDSDFFSKIVIEAITRVKT---

---RIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDELIIVKNSNKKSASI
ILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSIYLDNIAATMGSRKQ
LAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKKSYAYSGLDLFNNKV
RNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPREQQ
Translated Amino Acid sequence (All Frames) 
Frame A:
slhqtifiyiqyifiy*ikilkkecqtry**lmvivsvvmklghkmf*qlqqlqi*lkhh
ldqlv*ikc*liildqlllpmmvqpfykrli*niqqqrf*ynyqnckikklvmvqlql*f
*qqny*nvpmnw*hvkyiq*lllvvld*hvqkqsnistkh*qlklkpyqrisl*tlprhq
chqrpsmtivisfqrlslrqslv*k---

---RIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDELIIVKNSNKKSASI
ILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSIYLDNIAATMGSRKQ
LAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKKSYAYSGLDLFNNKV
RNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPREQQ

Frame B:
vstkrylytsniflyielkf*kknvkqgind*w*syqw**s*gtkcfssysnckys*nii
wtnwfr*nvn**ywinccyq*wcnhftkd*yrtssskdfstiiriarsrsw*wynyscnf
ssriietcq*igst*stsnsyy*wf*ismyrsnqiyqrniss*s*nltkgfhckhcqdin
vikdhq*r**flfkdch*gnhscen---

---vspnhvvvqs*shlqisrvknhlipppwvlpmklfkidwpmmn*slsktqirnqlqs
ffvvpmn*cwmkwnvpfmtlsvs*rel*nleplfqvvvqlnlpslsisiilppqwvvvnn
*qslnllnpswlyqnnwl*mlllmpli*fqn*kpiimplklihqrnlthtlvsiysitkl
eii*rlvf*nlplpksnvsnlqlkalspsfvsmikslliqennk

Frame C:
sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRISGNEVRAQNVLAVTAIANIVKTSF
GPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDGTTTVVIL
AAELLKRANELVARKVHPIVIISGFRLACTEAIKYINETLAVKVETLPKDFIVNIAKTSM
SSKTINDDSDFFSKIVIEAITRVKT---

---yrqimwwyslnhtcksrg*rii*yhrlgycr*scsr*igr**inhcqklk*eisfnh
sswcq*invg*ngtfhs*lslyrkenfriwnhcsrwwcs*icplylsr*ycrhng*s*ti
snl*ic*iplgctktigckccs*cl*fsfkikslsscrsn*sikeilrilwsrfiq*qs*
k*frgwcfrtchcqnqmyqicn*kryhhpsyr**nhs*skrttr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD589 (VFD589Q) /CSM/VF/VFD5-D/VFD589Q.Seq.d/                       2460   0.0
CFH434 (CFH434Q) /CSM/CF/CFH4-B/CFH434Q.Seq.d/                       1318   0.0
AFG366 (AFG366Q) /CSM/AF/AFG3-C/AFG366Q.Seq.d/                       1318   0.0
AFF451 (AFF451Q) /CSM/AF/AFF4-C/AFF451Q.Seq.d/                       1318   0.0
AFD439 (AFD439Q) /CSM/AF/AFD4-B/AFD439Q.Seq.d/                       1318   0.0
VFF578 (VFF578Q) /CSM/VF/VFF5-D/VFF578Q.Seq.d/                       1243   0.0
VFA730 (VFA730Q) /CSM/VF/VFA7-B/VFA730Q.Seq.d/                       1221   0.0
VFL307 (VFL307Q) /CSM/VF/VFL3-A/VFL307Q.Seq.d/                       1209   0.0
FC-AG03 (FC-AG03Q) /CSM/FC/FC-AG/FC-AG03Q.Seq.d/                     1183   0.0
FC-116 (FC-116Q) /CSM/CG/CG-FC/FC-116Q.Seq.d/                        1183   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB008158|AB008158.1 Dictyostelium discoideum DdTcp-1 mRNA for t-complex polypeptide 1 homologue, complete cds.  1318  0.0  3
AZ530054|AZ530054.1 ENTDW96TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66  8e-15  2
AZ526360|AZ526360.1 254PbF04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    70  2e-14  3
AZ522254|AZ522254.1 204PbE04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    68  1e-12  2
BF298951|BF298951.1 068PbG10 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68  1e-12  2
BF299139|BF299139.1 069PbF12 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68  1e-12  2
AL437502|AL437502.1 T3 end of clone BC0AA009G02 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    48  3e-10  4
AF143494|AF143494.1 Xenopus laevis t-complex polypeptide 1 (TCP1) mRNA, complete cds.    48  1e-08  5
U43123|U43123.1 Naegleria fowleri T-complex polypeptide homolog mRNA, partial cds.    38  9e-08  4
AF143496|AF143496.1 Paleosuchus palpebrosus t-complex polypeptide 1 (TCP1) mRNA, complete cds.    38  4e-07  5
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB008158_1(AB008158|pid:none) Dictyostelium discoideum DdTcp-1 m...   421   e-116
AF442545_1(AF442545|pid:none) Physarum polycephalum CCT chaperon...   319   1e-85
BT002052_1(BT002052|pid:none) Arabidopsis thaliana t-complex pol...   285   3e-75
(P18279) RecName: Full=T-complex protein 1 subunit alpha;       ...   283   9e-75
AY891878_1(AY891878|pid:none) Synthetic construct Homo sapiens c...   282   2e-74
(Q4R5G2) RecName: Full=T-complex protein 1 subunit alpha;       ...   282   2e-74
(P28480) RecName: Full=T-complex protein 1 subunit alpha;       ...   281   4e-74
AF143494_1(AF143494|pid:none) Xenopus laevis t-complex polypepti...   281   5e-74
(Q32L40) RecName: Full=T-complex protein 1 subunit alpha;       ...   280   6e-74
BC068901_1(BC068901|pid:none) Xenopus laevis t-complex polypepti...   280   1e-73
protein update 2009. 6.18
PSORT 

 psg: 0.57  gvh: 0.40  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 28.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VFD589 is cyt
5' end seq. ID VFD589F
5' end seq. 
>VFD589F.Seq
AGTCTCCACCAAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAAAATT
TTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGTAATG
AAGTTAGGGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCAT
TTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCA
ATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAG
TACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTT
TAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAATAG
TTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACAT
TAGCAGTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAA
TGTCATCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAA
TCACTCGTGTGAAAACC----------
Length of 5' end seq. 617
3' end seq. ID VFD589Z
3' end seq. 
>VFD589Z.Seq
----------CGTATCGCCAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAAATCT
CGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTCAAGA
TAGATTGGCCGATGATGAATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTTCAAT
CATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAACGTTCCATTCATGACTC
TCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTGCAGT
TGAATCTGCCCTCTCTATCTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTAAACA
ATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTGTAAA
TGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCA
AACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGT
TAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAA
ATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATCGATGATAAAATCACTCTTAATCC
AAGAGAACAACAAGG
Length of 3' end seq. 665
Connected seq. ID VFD589P
Connected seq. 
>VFD589P.Seq
AGTCTCCACCAAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAAAATT
TTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGTAATG
AAGTTAGGGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCAT
TTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCA
ATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAG
TACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTT
TAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAATAG
TTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACAT
TAGCAGTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAA
TGTCATCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAA
TCACTCGTGTGAAAACC----------CGTATCGCCAAATCATGTGGTGGTACAGTCTTA
ATCACACTTGCAAATCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGTATTGCC
GATGAAGTTGTTCAAGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAACTCAAAT
AAGAAATCAGCTTCAATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAA
CGTTCCATTCATGACTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTT
CCAGGTGGTGGTGCAGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCCGCCACA
ATGGGTAGTCGTAAACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCA
AAACAATTGGCTGTAAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCC
TATCATCATGCCGCTCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGAT
TTATTCAATAACAAAGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCC
AAAATCAAATGTATCAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATCGATGAT
AAAATCACTCTTAATCCAAGAGAACAACAAGG
Length of connected seq. 1282
Full length Seq ID -
Full length Seq. -
Length of full length seq. -