>VFD577 (VFD577Q) /CSM/VF/VFD5-D/VFD577Q.Seq.d/
AATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAXXXXXXXXXX

nhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYK---

Frame A:
nhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYK---


Frame B:
iiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigi
kkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnk
klivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttk---


Frame C:
ssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*
rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tk
s*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqr---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        803   0.0
VFM885 (VFM885Q) /CSM/VF/VFM8-D/VFM885Q.Seq.d/                        803   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        803   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        803   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        803   0.0
VFL283 (VFL283Q) /CSM/VF/VFL2-D/VFL283Q.Seq.d/                        803   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        803   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        803   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        803   0.0
VFJ261 (VFJ261Q) /CSM/VF/VFJ2-C/VFJ261Q.Seq.d/                        803   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   803  0.0  2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  2e-21  2
AW010688|AW010688.1 ST11A05 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST11A05, mRNA sequence.    46  0.016  2
BM158718|BM158718.1 NXLV_039_C04_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA clone NXLV_039_C04 5', mRNA sequence.    46  0.017  2
BM158719|BM158719.1 NXLV_039_C05_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA clone NXLV_039_C05 5', mRNA sequence.    46  0.017  2
AW043340|AW043340.1 ST32B12 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST32B12, mRNA sequence.    46  0.020  2
AW043146|AW043146.1 ST29H08 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST29H08, mRNA sequence.    46  0.020  2
CB076127|CB076127.1 hf37g09.g1 Hedyotis terminalis flower - Stage 2 (NYBG) Hedyotis terminalis cDNA clone hf37g09, mRNA sequence.    38  0.021  2
BX254774|BX254774.1 Pinus pinaster xylem EST, clone PP104C10.     46  0.022  2
CF390177|CF390177.1 RTDR2_12_D12.g1_A021 Loblolly pine roots recovering from drought DR2 Pinus taeda cDNA clone RTDR2_12_D12_A021 5', mRNA sequence.    46  0.022  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   285   6e-76
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   181   7e-45
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   179   3e-44
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   179   3e-44
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   179   3e-44
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   179   4e-44
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   179   5e-44
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   178   6e-44
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   177   1e-43
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   177   1e-43

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VFD577 is exc

>VFD577F.Seq
AATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGA----------