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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD576
Library VF
(Link to library)
Clone ID VFD576
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15826-1
Original site URL
Representative seq. ID VFD576P
(Link to Original site)
Representative DNA sequence 
>VFD576 (VFD576Q) /CSM/VF/VFD5-D/VFD576Q.Seq.d/
ATTTTTAGTTGAAAAAATATTATATAAAATGGTAAAGGGTAATAATAAGCCAACAAAGTT
AGGTGATACCTTAATTAAATCAAGAAATAAGAAACTAGGTAGAACATTCAATGAACAAAC
CATTGGTATGTTTACAACAGATATTGATCCAAAAGAAGAAAGAAAGAAATTAGTATCAAA
TACAGATACAGGTAATAATTTAGAGAATTTTTTAGAGATAACATCGTTAGAACAAAAAGT
ATTTGAAAGTGAGAAACAAAATGTAGTAGTTATAAGTAAAACATCATCTACAGTATTATC
AAAAGAAGAGAAAGAGAAAAAGACGAATGATCATCAAAAACTTATGGAAAAACATAGACT
CTATTGGAATTCATTGACTATTCCACGTAGACCACAATGGAATGAAAATACCACAACCGA
AGAATTGTTAGAATTGGAGAAAGAAGTATTTTATCATTGGAGAAAAGGTATTGCAAAGTT
GGAAGAGGAACAAGATTTATTGGTAACACCATTTGAAAAGAATGCTGAAGTTTGGAAACA
ATTGTGGCGTGTTGCTGAACGTTCCGATCTTTTAGTACAAXXXXXXXXXXTTNTGGTTNA
NCAAGTGATGATTTAACAGTTGNTGGTANAAAAGTTACATATGATTTCAATAAAGATAAT
GAAGCANNAATATATTTATCAAGATCAAGTTTCAGCTTTAACAAAAGATNAATCAAAGAA
AAAGAANAAAGTTATAAATTTACAAAAAGAACACATTGATTCAAATAAAAAAGATGCTTA
TACTCGTANTCAATTCCTTCATTCAACTACTCCTGAAGCTGCTAAATTAGAAAATATGTC
CAAAAAAGAAAAAGAAGAAAATTCAAGAAAACGTTTAGAACAAATTAAAGCTTTAAAACA
ACAAATTTTACAACAACAACAACAACAACAACCAACAACAACAACAACAACAACAACAAC
AACAACAACAACAACAACCG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
FLVEKILYKMVKGNNKPTKLGDTLIKSRNKKLGRTFNEQTIGMFTTDIDPKEERKKLVSN
TDTGNNLENFLEITSLEQKVFESEKQNVVVISKTSSTVLSKEEKEKKTNDHQKLMEKHRL
YWNSLTIPRRPQWNENTTTEELLELEKEVFYHWRKGIAKLEEEQDLLVTPFEKNAEVWKQ
LWRVAERSDLLVQ---

---XGXXSDDLTVXGXKVTYDFNKDNEAXIYLSRSSFSFNKRXIKEKEXSYKFTKRTH*f
k*krclysxsipsfnys*sc*irkyvqkrkrrkfkktfrtn*sfkttnfttttttttnnn
nnnnnnnnnnnn
Translated Amino Acid sequence (All Frames) 
Frame A:
ifs*knii*ngkg***ankvr*yln*ikk*etr*niq*tnhwyvynry*skrrkkeisik
yryr**freffrdnivrtksi*k*etkcssyk*niiysiikrrerekde*ssktygkt*t
llefidyst*ttme*kyhnrrivrigersilslekryckvgrgtrfignti*kec*slet
ivacc*tfrsfst---

---xwxxk**fnsxwxksyi*fq*r**sxnifikikfql*qkxnqrkrxkl*iykkntli
qikkmlilvxnsfiqlllklln*kicpkkkkkkiqenv*nklkl*nnkfynnnnnnnqqq
qqqqqqqqqqqqp

Frame B:
FLVEKILYKMVKGNNKPTKLGDTLIKSRNKKLGRTFNEQTIGMFTTDIDPKEERKKLVSN
TDTGNNLENFLEITSLEQKVFESEKQNVVVISKTSSTVLSKEEKEKKTNDHQKLMEKHRL
YWNSLTIPRRPQWNENTTTEELLELEKEVFYHWRKGIAKLEEEQDLLVTPFEKNAEVWKQ
LWRVAERSDLLVQ---

---XGXXSDDLTVXGXKVTYDFNKDNEAXIYLSRSSFSFNKRXIKEKEXSYKFTKRTH*f
k*krclysxsipsfnys*sc*irkyvqkrkrrkfkktfrtn*sfkttnfttttttttnnn
nnnnnnnnnnnn

Frame C:
f*lkkyyikw*rviisqqs*vip*lnqeirn*vehsmnkplvclqqiliqkkkern*yqi
qiqvii*rif*r*hr*nkkylkvrnkm**l*vkhhlqyyqkkrkrkrrmiiknlwknids
igih*lfhvdhngmkipqpknc*nwrkkyfiigekvlqswkrnkiyw*hhlkrmlkfgnn
cgvllnvpif*y---

---xvxqvmi*qlxvxklhmisikimkxxyiyqdqvsaltkdxskkkxkvinlqkehids
nkkdaytrxqflhsttpeaaklenmskkekeensrkrleqikalkqqilqqqqqqqpttt
tttttttttttt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD576 (VFD576Q) /CSM/VF/VFD5-D/VFD576Q.Seq.d/                       1548   0.0
VFD607 (VFD607Q) /CSM/VF/VFD6-A/VFD607Q.Seq.d/                       1126   0.0
SFK523 (SFK523Q) /CSM/SF/SFK5-A/SFK523Q.Seq.d/                       1126   0.0
CHC868 (CHC868Q) /CSM/CH/CHC8-C/CHC868Q.Seq.d/                       1126   0.0
CFK324 (CFK324Q) /CSM/CF/CFK3-A/CFK324Q.Seq.d/                       1120   0.0
CFA491 (CFA491Q) /CSM/CF/CFA4-D/CFA491Q.Seq.d/                       1057   0.0
CFF336 (CFF336Q) /CSM/CF/CFF3-B/CFF336Q.Seq.d/                       1015   0.0
CFG752 (CFG752Q) /CSM/CF/CFG7-C/CFG752Q.Seq.d/                        930   0.0
VFC214 (VFC214Q) /CSM/VF/VFC2-A/VFC214Q.Seq.d/                        791   0.0
SSE166 (SSE166Q) /CSM/SS/SSE1-C/SSE166Q.Seq.d/                        420   e-116
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AG061069|AG061069.1 Pan troglodytes DNA, clone: PTB-049A02.R.     42  0.002  2
BV028983|BV028983.1 S212P60182FB3.T0 CZECHII/Ei Mus musculus STS genomic, sequence tagged site.    36  0.028  3
AJ276790|AJ276790.1 Acrocephalus sechellensis microsatellite DNA, clone L5F12 (Ase 61).    38  0.035  3
AL161933|AL161933.11 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-275E20.    40  0.042  5
CC002137|CC002137.1 PUGJX87TB ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa401O06, DNA sequence.    34  0.045  3
AY193847|AY193847.1 Homo sapiens microsatellite 2-2 sequence.     36  0.049  3
AC094728|AC094728.5 Rattus norvegicus clone CH230-5H22, WORKING DRAFT SEQUENCE, 2 unordered pieces.    42  0.050  5
BG495853|BG495853.1 602540244F1 NIH_MGC_59 Homo sapiens cDNA clone IMAGE:4671394 5', mRNA sequence.    38  0.052  3
AC092301|AC092301.2 Homo sapiens chromosome 19 clone LLNLF-198C12, complete sequence.    40  0.062  2
AL592489|AL592489.13 Mouse DNA sequence from clone RP23-226J16 on chromosome 11.    46  0.068  3
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FM992693_198(FM992693|pid:none) Candida dubliniensis CD36 chromo...   107   6e-22
FN392319_491(FN392319|pid:none) Pichia pastoris GS115 chromosome...   107   8e-22
CP000498_204(CP000498|pid:none) Pichia stipitis CBS 6054 chromos...   107   8e-22
(Q10190) RecName: Full=Large subunit GTPase 1;          EC=3.6.1...   105   3e-21
CR382129_823(CR382129|pid:none) Yarrowia lipolytica strain CLIB1...   102   2e-20
(Q9W590) RecName: Full=Large subunit GTPase 1 homolog;          ...    99   2e-19
AP007150_500(AP007150|pid:none) Aspergillus oryzae RIB40 genomic...    99   2e-19
(P53145) RecName: Full=Large subunit GTPase 1;          EC=3.6.1...    97   6e-19
(Q6NY89) RecName: Full=Large subunit GTPase 1 homolog;          ...    97   8e-19
BC068833_1(BC068833|pid:none) Xenopus laevis hypothetical protei...    97   1e-18
protein update 2009. 6.18
PSORT 

 psg: 0.54  gvh: 0.24  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.07
 nuc: 0.03  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial

>> prediction for VFD576 is nuc
5' end seq. ID VFD576F
5' end seq. 
>VFD576F.Seq
ATTTTTAGTTGAAAAAATATTATATAAAATGGTAAAGGGTAATAATAAGCCAACAAAGTT
AGGTGATACCTTAATTAAATCAAGAAATAAGAAACTAGGTAGAACATTCAATGAACAAAC
CATTGGTATGTTTACAACAGATATTGATCCAAAAGAAGAAAGAAAGAAATTAGTATCAAA
TACAGATACAGGTAATAATTTAGAGAATTTTTTAGAGATAACATCGTTAGAACAAAAAGT
ATTTGAAAGTGAGAAACAAAATGTAGTAGTTATAAGTAAAACATCATCTACAGTATTATC
AAAAGAAGAGAAAGAGAAAAAGACGAATGATCATCAAAAACTTATGGAAAAACATAGACT
CTATTGGAATTCATTGACTATTCCACGTAGACCACAATGGAATGAAAATACCACAACCGA
AGAATTGTTAGAATTGGAGAAAGAAGTATTTTATCATTGGAGAAAAGGTATTGCAAAGTT
GGAAGAGGAACAAGATTTATTGGTAACACCATTTGAAAAGAATGCTGAAGTTTGGAAACA
ATTGTGGCGTGTTGCTGAACGTTCCGATCTTTTAGTACAA----------
Length of 5' end seq. 580
3' end seq. ID VFD576Z
3' end seq. 
>VFD576Z.Seq
----------TTNTGGTTNANCAAGTGATGATTTAACAGTTGNTGGTANAAAAGTTACAT
ATGATTTCAATAAAGATAATGAAGCANNAATATATTTATCAAGATCAAGTTTCAGCTTTA
ACAAAAGATNAATCAAAGAAAAAGAANAAAGTTATAAATTTACAAAAAGAACACATTGAT
TCAAATAAAAAAGATGCTTATACTCGTANTCAATTCCTTCATTCAACTACTCCTGAAGCT
GCTAAATTAGAAAATATGTCCAAAAAAGAAAAAGAAGAAAATTCAAGAAAACGTTTAGAA
CAAATTAAAGCTTTAAAACAACAAATTTTACAACAACAACAACAACAACAACCAACAACA
ACAACAACAACAACAACAACAACAACAACAACAACAACCG
Length of 3' end seq. 390
Connected seq. ID VFD576P
Connected seq. 
>VFD576P.Seq
ATTTTTAGTTGAAAAAATATTATATAAAATGGTAAAGGGTAATAATAAGCCAACAAAGTT
AGGTGATACCTTAATTAAATCAAGAAATAAGAAACTAGGTAGAACATTCAATGAACAAAC
CATTGGTATGTTTACAACAGATATTGATCCAAAAGAAGAAAGAAAGAAATTAGTATCAAA
TACAGATACAGGTAATAATTTAGAGAATTTTTTAGAGATAACATCGTTAGAACAAAAAGT
ATTTGAAAGTGAGAAACAAAATGTAGTAGTTATAAGTAAAACATCATCTACAGTATTATC
AAAAGAAGAGAAAGAGAAAAAGACGAATGATCATCAAAAACTTATGGAAAAACATAGACT
CTATTGGAATTCATTGACTATTCCACGTAGACCACAATGGAATGAAAATACCACAACCGA
AGAATTGTTAGAATTGGAGAAAGAAGTATTTTATCATTGGAGAAAAGGTATTGCAAAGTT
GGAAGAGGAACAAGATTTATTGGTAACACCATTTGAAAAGAATGCTGAAGTTTGGAAACA
ATTGTGGCGTGTTGCTGAACGTTCCGATCTTTTAGTACAA----------TTNTGGTTNA
NCAAGTGATGATTTAACAGTTGNTGGTANAAAAGTTACATATGATTTCAATAAAGATAAT
GAAGCANNAATATATTTATCAAGATCAAGTTTCAGCTTTAACAAAAGATNAATCAAAGAA
AAAGAANAAAGTTATAAATTTACAAAAAGAACACATTGATTCAAATAAAAAAGATGCTTA
TACTCGTANTCAATTCCTTCATTCAACTACTCCTGAAGCTGCTAAATTAGAAAATATGTC
CAAAAAAGAAAAAGAAGAAAATTCAAGAAAACGTTTAGAACAAATTAAAGCTTTAAAACA
ACAAATTTTACAACAACAACAACAACAACAACCAACAACAACAACAACAACAACAACAAC
AACAACAACAACAACAACCG
Length of connected seq. 970
Full length Seq ID -
Full length Seq. -
Length of full length seq. -