>AFB456 (AFB456Q) /CSM/AF/AFB4-C/AFB456Q.Seq.d/
XXXXXXXXXXAAATTCAATACCAATGATGTTCTCAACTTCAAATAGATTCGTTTATTATG
TACCAGCAAAATCAAATGACGATCCAAGATGGGATGCAGTTATCGATAAACTTCAAATGG
TTGGTAGAGGCAATGGTGGCGCTGTTAGAGGTATCTATAAAGGTGTTATTGAGACCACTT
TCTTTGGCAAAAGAGTTTATTTGGTTGGTACCTATTCACCATTCTATAGTCAACAACAAA
GAAATAAAAGTCAACGTATACTTTTCAATGATTTTCCAAAGGAAGAATTTGATCCAACCA
TCACAAATCCTTTAACAAGTACTATGCTATTTAGAACTGATAAAGATCTCTCATTGGATA
TTACTTTGGCTGAAAAAGTTTTCTCTTGTAAAGACTTTTGTGAAAAACATAAAGTATCAT
GTTTAGAATCTGATATGATGTTATTAACTGATAGTACTATAGTTTTACTACAATTGGAAC
AACATTGTAGTCAAGTAAACTATATACCAAGTAATAATACCGATACATTTTAT

---NSIPMMFSTSNRFVYYVPAKSNDDPRWDAVIDKLQMVGRGNGGAVRGIYKGVIETTF
FGKRVYLVGTYSPFYSQQQRNKSQRILFNDFPKEEFDPTITNPLTSTMLFRTDKDLSLDI
TLAEKVFSCKDFCEKHKVSCLESDMMLLTDSTIVLLQLEQHCSQVNYIPSNNTDTFY

Frame A:
---NSIPMMFSTSNRFVYYVPAKSNDDPRWDAVIDKLQMVGRGNGGAVRGIYKGVIETTF
FGKRVYLVGTYSPFYSQQQRNKSQRILFNDFPKEEFDPTITNPLTSTMLFRTDKDLSLDI
TLAEKVFSCKDFCEKHKVSCLESDMMLLTDSTIVLLQLEQHCSQVNYIPSNNTDTFY


Frame B:
---iqyq*csqlqidsfimyqqnqmtiqdgmqlsinfkwlveamvallevsikvllrpls
lakefiwlvpihhsivnnkeikvnvyfsmifqrknliqpsqil*qvlcylelikishwil
lwlkkfslvktfvknikyhv*nli*cy*livl*fyynwnnivvk*tiyqviipihf


Frame C:
---fntndvlnfk*irllctskik*rskmgcsyr*tsngw*rqwwrc*ryl*rcy*dhfl
wqkslfgwylftil*sttkk*kstytfq*fskgri*snhhksfnkyyai*n**rsligyy
fg*ksfll*rll*kt*simfri*ydvin**yysfttigttl*ssklytk**yryil

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFE435 (SFE435Q) /CSM/SF/SFE4-B/SFE435Q.Seq.d/                       1037   0.0
CHD312 (CHD312Q) /CSM/CH/CHD3-A/CHD312Q.Seq.d/                       1037   0.0
AFK872 (AFK872Q) /CSM/AF/AFK8-C/AFK872Q.Seq.d/                       1037   0.0
AFB456 (AFB456Q) /CSM/AF/AFB4-C/AFB456Q.Seq.d/                       1037   0.0
AFI627 (AFI627Q) /CSM/AF/AFI6-B/AFI627Q.Seq.d/                       1029   0.0
AFO831 (AFO831Q) /CSM/AF/AFO8-B/AFO831Q.Seq.d/                       1023   0.0
AFC207 (AFC207Q) /CSM/AF/AFC2-A/AFC207Q.Seq.d/                       1023   0.0
AHK215 (AHK215Q) /CSM/AH/AHK2-A/AHK215Q.Seq.d/                       1021   0.0
AFF364 (AFF364Q) /CSM/AF/AFF3-C/AFF364Q.Seq.d/                       1021   0.0
CFG834 (CFG834Q) /CSM/CF/CFG8-B/CFG834Q.Seq.d/                       1009   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AI773934|AI773934.1 EST255034 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER8N1, mRNA sequence.    38  0.57  2
BG133859|BG133859.1 EST466751 tomato crown gall Lycopersicon esculentum cDNA clone cTOE14I10 5' sequence, mRNA sequence.    38  0.69  2
AI490786|AI490786.1 EST241494 tomato shoot, Cornell Lycopersicon esculentum cDNA clone cLEB3H9 similar to cinnamic acid 4-hydroxylase, mRNA sequence.    38  0.82  2
BG128254|BG128254.1 EST473900 tomato shoot/meristem Lycopersicon esculentum cDNA clone cTOF19D15 5' sequence, mRNA sequence.    38  0.91  2
AI484136|AI484136.1 EST248943 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER1C5, mRNA sequence.    38  1.0  2
AE003601|AE003601.3 Drosophila melanogaster chromosome 3R, section 7 of 118 of the complete sequence.    42  1.5  3
AZ299204|AZ299204.1 RPCI-23-165H3.TV RPCI-23 Mus musculus genomic clone RPCI-23-165H3, DNA sequence.    44  1.7  1
BX323843|BX323843.6 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-10A23.    46  3.3  2
AL163702|AL163702.1 S.pombe chromosome II P1 p35G2.               38  3.3  2
AL844583|AL844583.6 Mouse DNA sequence from clone RP23-240F13 on chromosome X.    40  3.4  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Y17877_1(Y17877|pid:none) Sycon raphanus mRNA for insulin recept...    36   0.65
BT011349_1(BT011349|pid:none) Drosophila melanogaster RH02355 fu...    35   0.85
AY069808_1(AY069808|pid:none) Drosophila melanogaster SD04942 fu...    35   0.85
AE014297_4620(AE014297|pid:none) Drosophila melanogaster chromos...    35   0.85
CP001175_2092(CP001175|pid:none) Listeria monocytogenes HCC23, c...    35   1.5
AC009322_3(AC009322|pid:none) Arabidopsis thaliana chromosome I ...    34   1.9
AX814538_1(AX814538|pid:none) Sequence 19 from Patent WO03064471.      33   3.2
DQ812518_2(DQ812518|pid:none) Spironucleus barkhanus isolate Sp1...    33   3.2
AJ010592_91(AJ010592|pid:none) Guillardia theta DNA for complete...    33   3.2
FM992689_214(FM992689|pid:none) Candida dubliniensis CD36 chromo...    33   4.2

 psg: 0.92  gvh: 0.39  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for AFB456 is cyt

>AFB456Z.Seq
----------AAATTCAATACCAATGATGTTCTCAACTTCAAATAGATTCGTTTATTATG
TACCAGCAAAATCAAATGACGATCCAAGATGGGATGCAGTTATCGATAAACTTCAAATGG
TTGGTAGAGGCAATGGTGGCGCTGTTAGAGGTATCTATAAAGGTGTTATTGAGACCACTT
TCTTTGGCAAAAGAGTTTATTTGGTTGGTACCTATTCACCATTCTATAGTCAACAACAAA
GAAATAAAAGTCAACGTATACTTTTCAATGATTTTCCAAAGGAAGAATTTGATCCAACCA
TCACAAATCCTTTAACAAGTACTATGCTATTTAGAACTGATAAAGATCTCTCATTGGATA
TTACTTTGGCTGAAAAAGTTTTCTCTTGTAAAGACTTTTGTGAAAAACATAAAGTATCAT
GTTTAGAATCTGATATGATGTTATTAACTGATAGTACTATAGTTTTACTACAATTGGAAC
AACATTGTAGTCAAGTAAACTATATACCAAGTAATAATACCGATACATTTTAT