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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD455
Library VF
(Link to library)
Clone ID VFD455
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15854-1
Original site URL
Representative seq. ID VFD455Z
(Link to Original site)
Representative DNA sequence 
>VFD455 (VFD455Q) /CSM/VF/VFD4-C/VFD455Q.Seq.d/
XXXXXXXXXXTTNCCATAAATCAAAAGCTGTCCATGGTATTGTTAGATATGTAGCAACTA
ANCTTTCAACCGAAGATAATGTAGTTAAAACTAAACACTTATACACTAAATTCGTTTGGC
CATTATACACAAAATATGGTCATGCTTATGAAGCTTTTAAATTATCAATTACTGAACCAT
CAGTATTTAATGGATTTGATATTGCAGAGAATGAAAGAAAAGTATTAATGGAAACCATTA
TCCAAAAACTTAAACCACAACCACATAAAGTACGTGCTGATTTAGAGATTACATGTTATG
ATTATGAAGGTATTGATGCTATTAAACACGCAATCACTGCCAGTCAAAATCACGCATTAT
CTGTGCTCAAAGCACCAGAAGATGGTAAATTTGATGAAAAAGCATTTGGTGTCGTTACAA
TTAAACTCGTCGCTCCACCACTCTACGTTATGGTTGGTACTTTTGATGAAAAAGAAAAAG
GTCTTTCAATGGTTGGTCAATGTGTTGATGTCTTAAGTGAAGAAATTACCAAAAAGAATG
GTAATCTTACAATTAAAGCTGCTCCACGTATTGTTGGTGCTGTTGATGACCAAGAACTTC
GTGATCTTATGGAACAATTAGAAGTTGAAAATCAAGATGGTGATGGTGAAGAACATGAAG
ATGATGATGACGATGACGAGATGAAGAGGAAGAAGAAAAACCAAAGGAAAAGAAATCGAG
CAGAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---XHKSKAVHGIVRYVATXLSTEDNVVKTKHLYTKFVWPLYTKYGHAYEAFKLSITEPS
VFNGFDIAENERKVLMETIIQKLKPQPHKVRADLEITCYDYEGIDAIKHAITASQNHALS
VLKAPEDGKFDEKAFGVVTIKLVAPPLYVMVGTFDEKEKGLSMVGQCVDVLSEEITKKNG
NLTIKAAPRIVGAVDDQELRDLMEQLEVENQDGDGEEHEDDDDDDEMKRKKKNQRKRNRA
E
Translated Amino Acid sequence (All Frames) 
Frame A:
---XHKSKAVHGIVRYVATXLSTEDNVVKTKHLYTKFVWPLYTKYGHAYEAFKLSITEPS
VFNGFDIAENERKVLMETIIQKLKPQPHKVRADLEITCYDYEGIDAIKHAITASQNHALS
VLKAPEDGKFDEKAFGVVTIKLVAPPLYVMVGTFDEKEKGLSMVGQCVDVLSEEITKKNG
NLTIKAAPRIVGAVDDQELRDLMEQLEVENQDGDGEEHEDDDDDDEMKRKKKNQRKRNRA
E


Frame B:
---xinqklsmvlldm*qlxfqpkim*lklntytlnsfghytqnmvmlmkllnyqllnhq
ylmdlilqrmkeky*wkplsknlnhnhikyvli*rlhvmimkvlmllntqslpvkithyl
cskhqkmvnlmkkhlvslqlnsslhhstlwlvllmkkkkvfqwlvnvlms*vkklpkrmv
ilqlkllhvllvllmtknfvilwnn*klkikmvmvknmkmmmtmtr*rgrrktkgkeieq
k


Frame C:
---p*ikscpwyc*icsn*xfnrr*cs*n*tlih*irlaiihkiwscl*sf*iiny*tis
i*wi*ycre*kksingnhypkt*tttt*stc*frdyml*l*ry*cy*trnhcqsksriic
aqstrrw*i**ksiwcryn*trrsttlrygwyf**krkrsfngwsmc*clk*rnyqkew*
syn*scstycwcc**prts*sygtirs*ksrw*w*rt*r***r*rdeeeeekpkekkssr
k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD455 (VFD455Q) /CSM/VF/VFD4-C/VFD455Q.Seq.d/                       1237   0.0
VFE187 (VFE187Q) /CSM/VF/VFE1-D/VFE187Q.Seq.d/                       1233   0.0
SFI105 (SFI105Q) /CSM/SF/SFI1-A/SFI105Q.Seq.d/                       1229   0.0
SFG256 (SFG256Q) /CSM/SF/SFG2-C/SFG256Q.Seq.d/                       1138   0.0
VFG366 (VFG366Q) /CSM/VF/VFG3-C/VFG366Q.Seq.d/                        961   0.0
VHL179 (VHL179Q) /CSM/VH/VHL1-D/VHL179Q.Seq.d/                        888   0.0
SFK730 (SFK730Q) /CSM/SF/SFK7-B/SFK730Q.Seq.d/                        813   0.0
VFI707 (VFI707Q) /CSM/VF/VFI7-A/VFI707Q.Seq.d/                        500   e-140
VFI121 (VFI121Q) /CSM/VF/VFI1-A/VFI121Q.Seq.d/                        293   2e-78
VFA872 (VFA872Q) /CSM/VF/VFA8-C/VFA872Q.Seq.d/                         40   0.046
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115682|AC115682.2 Dictyostelium discoideum chromosome 2 map 4101105-4158643 strain AX4, complete sequence.   775  0.0  5
BM163678|BM163678.1 EST566201 PyBS Plasmodium yoelii yoelii cDNA clone PYCLI25 5' end, mRNA sequence.    40  2e-05  3
BM169195|BM169195.1 EST571718 PyBS Plasmodium yoelii yoelii cDNA clone PYCPN31 5' end, mRNA sequence.    40  8e-05  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36  0.006  9
BM168993|BM168993.1 EST571516 PyBS Plasmodium yoelii yoelii cDNA clone PYCPK40 5' end, mRNA sequence.    40  0.010  2
BG127028|BG127028.1 EST472674 tomato shoot/meristem Lycopersicon esculentum cDNA clone cTOF14M7 5' sequence, mRNA sequence.    48  0.15  1
AW622118|AW622118.1 EST312916 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX14E16 5', mRNA sequence.    48  0.15  1
CA517926|CA517926.1 KS10002H01 KS10 Capsicum annuum cDNA, mRNA sequence.    48  0.15  1
AW093182|AW093182.1 EST286362 tomato mixed elicitor, BTI Lycopersicon esculentum cDNA clone cLET23P4, mRNA sequence.    48  0.15  1
AI484362|AI484362.1 EST249010 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES1G15 similar to eukaryotic translation initiation factor 2 alpha subunit (EIF-2-alpha), mRNA sequence.    48  0.15  1
dna update 2003. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869N9) RecName: Full=Eukaryotic translation initiation factor ...   412   e-114
(P56286) RecName: Full=Eukaryotic translation initiation factor ...   151   2e-35
AM270024_1(AM270024|pid:none) Aspergillus niger contig An02c0290...   142   1e-32
(Q6GL89) RecName: Full=Eukaryotic translation initiation factor ...   139   1e-31
(P05198) RecName: Full=Eukaryotic translation initiation factor ...   138   2e-31
EU716630_1(EU716630|pid:none) Synthetic construct N-EGFP/eukaryo...   138   2e-31
(P68101) RecName: Full=Eukaryotic translation initiation factor ...   138   2e-31
AY891815_1(AY891815|pid:none) Synthetic construct Homo sapiens c...   138   2e-31
AF257517_1(AF257517|pid:none) Danio rerio eIF2 alpha subunit mRN...   137   3e-31
(Q7ZTK4) RecName: Full=Eukaryotic translation initiation factor ...   137   3e-31
protein update 2009. 6.18
PSORT 

 psg: 0.85  gvh: 0.32  alm: 0.33  top: 0.23  tms: 0.00  mit: 0.31  mip: 0.03
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFD455 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VFD455Z
3' end seq. 
>VFD455Z.Seq
----------TTNCCATAAATCAAAAGCTGTCCATGGTATTGTTAGATATGTAGCAACTA
ANCTTTCAACCGAAGATAATGTAGTTAAAACTAAACACTTATACACTAAATTCGTTTGGC
CATTATACACAAAATATGGTCATGCTTATGAAGCTTTTAAATTATCAATTACTGAACCAT
CAGTATTTAATGGATTTGATATTGCAGAGAATGAAAGAAAAGTATTAATGGAAACCATTA
TCCAAAAACTTAAACCACAACCACATAAAGTACGTGCTGATTTAGAGATTACATGTTATG
ATTATGAAGGTATTGATGCTATTAAACACGCAATCACTGCCAGTCAAAATCACGCATTAT
CTGTGCTCAAAGCACCAGAAGATGGTAAATTTGATGAAAAAGCATTTGGTGTCGTTACAA
TTAAACTCGTCGCTCCACCACTCTACGTTATGGTTGGTACTTTTGATGAAAAAGAAAAAG
GTCTTTCAATGGTTGGTCAATGTGTTGATGTCTTAAGTGAAGAAATTACCAAAAAGAATG
GTAATCTTACAATTAAAGCTGCTCCACGTATTGTTGGTGCTGTTGATGACCAAGAACTTC
GTGATCTTATGGAACAATTAGAAGTTGAAAATCAAGATGGTGATGGTGAAGAACATGAAG
ATGATGATGACGATGACGAGATGAAGAGGAAGAAGAAAAACCAAAGGAAAAGAAATCGAG
CAGAAAA
Length of 3' end seq. 717
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -