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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD443
Library VF
(Link to library)
Clone ID VFD443
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16445-1
Original site URL
Representative seq. ID VFD443E
(Link to Original site)
Representative DNA sequence 
>VFD443 (VFD443Q) /CSM/VF/VFD4-B/VFD443Q.Seq.d/
AATTTAATTTAAAATAAAAACATATTACATACAAATAAAATGAATAAAATTTTAACTCGT
AGTTTTAGTACTGGTGCAAATTTCTTTGCCTCTGGTAAAGGTAAAGCACCAGTTAGAGTT
GCCATTACTGGTGCCAGTGGTCAAATTGGTTATCAATTATTATTCAGAATTGCCAGTGGT
GATATGTTAGGTAAAGATCAACCAATCATTCTCCAATGTTTAGAATTACCAGGTGCAATG
AACTCATTAAAAGGTGTTTCAATGGAATTGGATGATTGTGCATTCCCATTGCTCAAAGGT
ATTGTTCAAAGTGATAAACCAGAGGAAGCATTCGCTGGTGCAGACTATGCATTATTAGTT
GGTGCTCGTCCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAA
ATTTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTT
TTAGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAAT
ATTGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACAACAGAGGTTTAGCTCAA
TTAGCCGATAAAACTGGTTCAGCCGTAACTGATATTGAAAAATTCTGCATTTGGGGTAAC
CACTCTGCCACCCAATACCCAGATATTAACTTTGGTACCGTCAAAGGTAAATCACTCGTA
GATACCATCAACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGA
GGTGCCGCTATTATTGCTGCCCGTGGTTTATCATCAGCTGCCTCTGCTGCTTCAGCTGCC
ATCGATCACATGAGAGATTGGACCTATGGTACCAATGGTCAATGGACCTCTATGGCCATT
TACTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTA
GATAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTTGATCAATTCTCTCAAGAA
CGTTTCGATGCTACTCGTAAAGAATTATTATCAGAAATGGATGGTGTTAAAGAATTATTA
CCATAAATTAAATTTTAAAAATAAAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
nli*NKNILHTNKMNKILTRSFSTGANFFASGKGKAPVRVAITGASGQIGYQLLFRIASG
DMLGKDQPIILQCLELPGAMNSLKGVSMELDDCAFPLLKGIVQSDKPEEAFAGADYALLV
GARPRSKGMERGDLLKANAEIFSVQGKALDKSANRDTLRVLVVGNPANTNALIAARNAPN
IDPKRFSAMTRLDHNRGLAQLADKTGSAVTDIEKFCIWGNHSATQYPDINFGTVKGKSLV
DTINDQKWVKDNFIPTVQQRGAAIIAARGLSSAASAASAAIDHMRDWTYGTNGQWTSMAI
YSEGEYGADKGLYFSFPVIVDNKGKYEIVKGLKLDQFSQERFDATRKELLSEMDGVKELL
P*ikf*k*k
Translated Amino Acid sequence (All Frames) 
Frame A:
nli*NKNILHTNKMNKILTRSFSTGANFFASGKGKAPVRVAITGASGQIGYQLLFRIASG
DMLGKDQPIILQCLELPGAMNSLKGVSMELDDCAFPLLKGIVQSDKPEEAFAGADYALLV
GARPRSKGMERGDLLKANAEIFSVQGKALDKSANRDTLRVLVVGNPANTNALIAARNAPN
IDPKRFSAMTRLDHNRGLAQLADKTGSAVTDIEKFCIWGNHSATQYPDINFGTVKGKSLV
DTINDQKWVKDNFIPTVQQRGAAIIAARGLSSAASAASAAIDHMRDWTYGTNGQWTSMAI
YSEGEYGADKGLYFSFPVIVDNKGKYEIVKGLKLDQFSQERFDATRKELLSEMDGVKELL
P*ikf*k*k


Frame B:
i*fkiktyyiqik*ikf*lvvlvlvqislplvkvkhqlelpllvpvvklvinyyselpvv
ic*vkinqsfsnv*nyqvq*th*kvfqwnwmivhshcskvlfkvinqrkhslvqtmhy*l
vlvqearvwsvvtf*kqmlkfslfkvkh*tkvlivtl*ef*l*vtqpipth*lppvmhqi
ltqnvslp*lv*ittev*ln*piklvqp*lilknsafgvttlppntqiltlvpskvnhs*
ipstiknglkitlfqpfnkevpllllpvvyhqlplllqlpsit*eigpmvpmvngplwpf
tlkvnmvpikvstslsqsl*itkvntkslkvlnlinslknvsmllvknyyqkwmvlknyy
hklnfknk


Frame C:
fnlk*khityk*ne*nfns*f*ywckflclw*r*sts*schywcqwsnwlsiiiqncqw*
yvr*rstnhspmfritrcnelikrcfngig*lcipiaqrycsk**trgsirwcrlciisw
csskkqgygaw*pfkskc*nflcsr*sirqkc*s*hfksfscr*psqyqrincrp*ctky
*pktflchdsfrsqqrfssisr*nwfsrn*y*kilhlg*plchpipry*lwyrqr*itrr
yhqrskmg*r*lysnrstkrcryyccpwfiisclccfschrsherldlwyqwsmdlyghl
l*r*iwcr*rslllfpshcr*qr*irnr*rs*t*silsrtfrcys*riiirngwc*riit
in*ilkik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD532 (VSD532Q) /CSM/VS/VSD5-B/VSD532Q.Seq.d/                       2093   0.0
VFH801 (VFH801Q) /CSM/VF/VFH8-A/VFH801Q.Seq.d/                       2093   0.0
VFD443 (VFD443Q) /CSM/VF/VFD4-B/VFD443Q.Seq.d/                       2093   0.0
SFK802 (SFK802Q) /CSM/SF/SFK8-A/SFK802Q.Seq.d/                       2093   0.0
CHD266 (CHD266Q) /CSM/CH/CHD2-C/CHD266Q.Seq.d/                       2093   0.0
CHB451 (CHB451Q) /CSM/CH/CHB4-C/CHB451Q.Seq.d/                       2077   0.0
SFK837 (SFK837Q) /CSM/SF/SFK8-B/SFK837Q.Seq.d/                       2076   0.0
CHE490 (CHE490Q) /CSM/CH/CHE4-D/CHE490Q.Seq.d/                       2066   0.0
VFC762 (VFC762Q) /CSM/VF/VFC7-C/VFC762Q.Seq.d/                       2064   0.0
SFJ115 (SFJ115Q) /CSM/SF/SFJ1-A/SFJ115Q.Seq.d/                       2062   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AR317645|AR317645.1 Sequence 195 from patent US 6562958.          38  7e-09  5
AX685430|AX685430.1 Sequence 43 from Patent WO02090383.           42  1e-06  4
AF288743|AF288743.1 Trichomonas tenax putative cytosolic malate dehydrogenase (MDH) mRNA, partial cds.    38  6e-04  3
AX067436|AX067436.1 Sequence 11 from Patent WO0078968.            42  0.002  4
AL645638|AL645638.10 Human DNA sequence from clone RP11-137D19 on chromosome X.    48  0.022  3
AC090787|AC090787.7 Homo sapiens chromosome 18, clone RP11-373G16, complete sequence.    48  0.24  1
AC104071|AC104071.3 Homo sapiens BAC clone RP11-315A17 from 4, complete sequence.    48  0.24  1
AC080190|AC080190.4 Homo sapiens chromosome 7 clone RP11-962B9, WORKING DRAFT SEQUENCE, 13 unordered pieces.    48  0.24  1
AC020734|AC020734.6 Homo sapiens chromosome 4 clone RP11-665I14, complete sequence.    48  0.24  1
AC119478|AC119478.8 Rattus norvegicus clone CH230-180D3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48  0.24  1
dna update 2003. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54D04) RecName: Full=Probable malate dehydrogenase 2, mitochon...   647   0.0
(Q0ABE6) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   386   e-106
CP000934_1926(CP000934|pid:none) Cellvibrio japonicus Ueda107, c...   373   e-102
(A6SY47) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   370   e-101
CP001614_1530(CP001614|pid:none) Teredinibacter turnerae T7901, ...   370   e-101
CP001053_2776(CP001053|pid:none) Burkholderia phytofirmans PsJN ...   368   e-100
(Q1LKG0) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   367   e-100
(Q393V1) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   367   e-100
(A1UZ10) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   366   e-100
(Q0K8F5) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   366   e-100
protein update 2009. 7.10
PSORT 

 psg: 0.60  gvh: 0.46  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VFD443 is cyt
5' end seq. ID VFD443F
5' end seq. 
>VFD443F.Seq
AATTTAATTTAAAATAAAAACATATTACATACAAATAAAATGAATAAAATTTTAACTCGT
AGTTTTAGTACTGGTGCAAATTTCTTTGCCTCTGGTAAAGGTAAAGCACCAGTTAGAGTT
GCCATTACTGGTGCCAGTGGTCAAATTGGTTATCAATTATTATTCAGAATTGCCAGTGGT
GATATGTTAGGTAAAGATCAACCAATCATTCTCCAATGTTTAGAATTACCAGGTGCAATG
AACTCATTAAAAGGTGTTTCAATGGAATTGGATGATTGTGCATTCCCATTGCTCAAAGGT
ATTGTTCAAAGTGATAAACCAGAGGAAGCATTCGCTGGTGCAGACTATGCATTATTAGTT
GGTGCTCGTCCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAA
ATTTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTT
TTAGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAAT
ATTGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACA----------
Length of 5' end seq. 583
3' end seq. ID VFD443Z
3' end seq. 
>VFD443Z.Seq
----------AGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAAAT
TTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTTTT
AGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAATAT
TGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACAACAGAGGTTTAGCTCAATT
AGCCGATAAAACTGGTTCAGCCGTAACTGATATTGAAAAATTCTGCATTTGGGGTAACCA
CTCTGCCACCCAATACCCAGATATTAACTTTGGTACCGTCAAAGGTAAATCACTCGTAGA
TACCATCAACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGAGG
TGCCGCTATTATTGCTGCCCGTGGTTTATCATCAGCTGCCTCTGCTGCTTCAGCTGCCAT
CGATCACATGAGAGATTGGACCTATGGTACCAATGGTCAATGGACCTCTATGGCCATTTA
CTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTAGA
TAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTTGATCAATTCTCTCAAGAACG
TTTCGATGCTACTCGTAAAGAATTATTATCAGAAATGGATGGTGTTAAAGAATTATTACC
ATAAATTAAATTTTAAAAATAAAAA
Length of 3' end seq. 735
Connected seq. ID VFD443P
Connected seq. 
>VFD443P.Seq
AATTTAATTTAAAATAAAAACATATTACATACAAATAAAATGAATAAAATTTTAACTCGT
AGTTTTAGTACTGGTGCAAATTTCTTTGCCTCTGGTAAAGGTAAAGCACCAGTTAGAGTT
GCCATTACTGGTGCCAGTGGTCAAATTGGTTATCAATTATTATTCAGAATTGCCAGTGGT
GATATGTTAGGTAAAGATCAACCAATCATTCTCCAATGTTTAGAATTACCAGGTGCAATG
AACTCATTAAAAGGTGTTTCAATGGAATTGGATGATTGTGCATTCCCATTGCTCAAAGGT
ATTGTTCAAAGTGATAAACCAGAGGAAGCATTCGCTGGTGCAGACTATGCATTATTAGTT
GGTGCTCGTCCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAA
ATTTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTT
TTAGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAAT
ATTGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACA----------AGAAGCA
AGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTTCTCTGTTCAAGGTA
AAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGTTGTAGGTAACCCAG
CCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAATATTGACCCAAAACGTTTCT
CTGCCATGACTCGTTTAGATCACAACAGAGGTTTAGCTCAATTAGCCGATAAAACTGGTT
CAGCCGTAACTGATATTGAAAAATTCTGCATTTGGGGTAACCACTCTGCCACCCAATACC
CAGATATTAACTTTGGTACCGTCAAAGGTAAATCACTCGTAGATACCATCAACGATCAAA
AATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGAGGTGCCGCTATTATTGCTG
CCCGTGGTTTATCATCAGCTGCCTCTGCTGCTTCAGCTGCCATCGATCACATGAGAGATT
GGACCTATGGTACCAATGGTCAATGGACCTCTATGGCCATTTACTCTGAAGGTGAATATG
GTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTAGATAACAAAGGTAAATACG
AAATCGTTAAAGGTCTTAAACTTGATCAATTCTCTCAAGAACGTTTCGATGCTACTCGTA
AAGAATTATTATCAGAAATGGATGGTGTTAAAGAATTATTACCATAAATTAAATTTTAAA
AATAAAAA
Length of connected seq. 1318
Full length Seq ID VFD443E
Full length Seq. 
>VFD443E.Seq
AATTTAATTTAAAATAAAAACATATTACATACAAATAAAATGAATAAAATTTTAACTCGT
AGTTTTAGTACTGGTGCAAATTTCTTTGCCTCTGGTAAAGGTAAAGCACCAGTTAGAGTT
GCCATTACTGGTGCCAGTGGTCAAATTGGTTATCAATTATTATTCAGAATTGCCAGTGGT
GATATGTTAGGTAAAGATCAACCAATCATTCTCCAATGTTTAGAATTACCAGGTGCAATG
AACTCATTAAAAGGTGTTTCAATGGAATTGGATGATTGTGCATTCCCATTGCTCAAAGGT
ATTGTTCAAAGTGATAAACCAGAGGAAGCATTCGCTGGTGCAGACTATGCATTATTAGTT
GGTGCTCGTCCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAA
ATTTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTT
TTAGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAAT
ATTGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACAACAGAGGTTTAGCTCAA
TTAGCCGATAAAACTGGTTCAGCCGTAACTGATATTGAAAAATTCTGCATTTGGGGTAAC
CACTCTGCCACCCAATACCCAGATATTAACTTTGGTACCGTCAAAGGTAAATCACTCGTA
GATACCATCAACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGA
GGTGCCGCTATTATTGCTGCCCGTGGTTTATCATCAGCTGCCTCTGCTGCTTCAGCTGCC
ATCGATCACATGAGAGATTGGACCTATGGTACCAATGGTCAATGGACCTCTATGGCCATT
TACTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTA
GATAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTTGATCAATTCTCTCAAGAA
CGTTTCGATGCTACTCGTAAAGAATTATTATCAGAAATGGATGGTGTTAAAGAATTATTA
CCATAAATTAAATTTTAAAAATAAAAA
Length of full length seq. 1107