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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD246
Library VF
(Link to library)
Clone ID VFD246
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16046-1
Original site URL
Representative seq. ID VFD246E
(Link to Original site)
Representative DNA sequence 
>VFD246 (VFD246Q) /CSM/VF/VFD2-B/VFD246Q.Seq.d/
ACCACGATGAGAATTATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCA
TCATCATCATCCTCTTCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAA
ATTGCACCAAGACAAGAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTAT
TATTGGAATCAATTAACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTA
TGTATTGCACATGAAAATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAAT
CTTGGTAATTCAAGTTATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGT
ACATTTACAGATGGTTTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCT
CAACAATATACAGAATTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTC
TATCAAATTTCAATCGATGTAGCATGGTCACCATCATGCATTGATAAACGTTGTTATGAT
TATGCTGGTTTAGCTTCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGT
CAAGTTTTTGGTGAGAAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTCAAATGCCTTG
GCTGGTATAAATAATTTTACTGATTTAGGTATTAGCACTGATCAATTGGTAATGGGTTTA
CCATGGTATGGTTATATTTATAAGAATTGTTTAAATGGTGATGAAGCAGGTTTGGAAACT
GTTGTTTGTCAAATTGAATCTGTACCATTTAGAGGTGCAAATTGTTCAGATGCAGCTGGT
TCAGAATATGATTATAGTTATTTGGTTCAATTATTACAAGATCAAACTATAAACTCTTCT
GCAGTTCAATGGAATACTGAATGGCAATCACCTTATTTTAATTATATTGACCCTATAACT
GGTAATGTTGATCAAGTTTGGTTTGATAATCCACAATCATTATCAATTAAAGTTCAATTA
GCTCAAAAATTAAATTTAAGAGGTGTTGCAGTTTGGAATATTGATTTTTTAGATTTTTCA
GATCAATACAATTCTCGTCCAATGTGGGATGCATTAGCTTCTTTTTTCCCACAATC
sequence update 2001. 6. 9
Translated Amino Acid sequence 
TTMRIILLLFLIVFVVAQSSSSSSSSGCPCDSSYLCEPLQIAPRQEFLGFSLNSTQYPNY
YWNQLTTLAIFYETIEDELLCIAHENDVRLVWGTTFPIENLGNSSYIEEWIQEQIEKVQS
TFTDGLNFDVESPITDPTIAQQYTELVSATNKAFKAINPFYQISIDVAWSPSCIDKRCYD
YAGLASNSDFLVAMDYDERSQVFGEKVCTAGANSSPSNALAGINNFTDLGISTDQLVMGL
PWYGYIYKNCLNGDEAGLETVVCQIESVPFRGANCSDAAGSEYDYSYLVQLLQDQTINSS
AVQWNTEWQSPYFNYIDPITGNVDQVWFDNPQSLSIKVQLAQKLNLRGVAVWNIDFLDFS
DQYNSRPMWDALASFFPQ
Translated Amino Acid sequence (All Frames) 
Frame A:
TTMRIILLLFLIVFVVAQSSSSSSSSGCPCDSSYLCEPLQIAPRQEFLGFSLNSTQYPNY
YWNQLTTLAIFYETIEDELLCIAHENDVRLVWGTTFPIENLGNSSYIEEWIQEQIEKVQS
TFTDGLNFDVESPITDPTIAQQYTELVSATNKAFKAINPFYQISIDVAWSPSCIDKRCYD
YAGLASNSDFLVAMDYDERSQVFGEKVCTAGANSSPSNALAGINNFTDLGISTDQLVMGL
PWYGYIYKNCLNGDEAGLETVVCQIESVPFRGANCSDAAGSEYDYSYLVQLLQDQTINSS
AVQWNTEWQSPYFNYIDPITGNVDQVWFDNPQSLSIKVQLAQKLNLRGVAVWNIDFLDFS
DQYNSRPMWDALASFFPQ


Frame B:
pr*elfycff*lfslllkvhhhhplqdvhvihhiyvnhfklhqdksf*vfh*iarniqii
igin*qhlryfmkqlkmnyyvlhmkmmld*ygvqhfqlkilviqvilkngfknklkkfkv
hlqmv*ilmlkvqlqiqpslnniqn*yqqlikhlkqlihsikfqsm*hghhhalinvvmi
mlv*lqipisl*qwtmmrevkflvrrcvllvqilhpqmpwlv*iilli*vlalinw*wvy
hgmvifiriv*mvmkqvwkllfvklnlyhlevqivqmqlvqnmiiviwfnyykikl*tll
qfngilngnhliliiltl*lvmlikfgliihnhyqlkfn*lkn*i*evlqfgilif*ifq
intilvqcgmh*llfshn


Frame C:
hdenyfiaffncfrccskfiiiilfrmsm*fiifm*ttsnctktrvfrffik*haiskll
lesinntcdil*nn*r*iimyct*k*c*ismgynisn*ksw*fkly*rmdsrtn*kssky
iyrwfkf*c*ksnyrsnhrstiyrisisn**si*sn*silsnfnrcsmvtimh**tll*l
cwfsfkfrfpcsngl**ekssfw*egvycwckfftlkclgwyk*fy*fry*h*signgft
mvwlyl*elfkw**srfgncclsn*icti*rcklfrcswfri*l*lfgsiitrsnyklfc
ssmey*maitlf*ly*pynw*c*sslv**stiiin*ssisskikfkrccsley*ffrffr
siqfssnvgcisfffpti
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD280 (VFD280Q) /CSM/VF/VFD2-D/VFD280Q.Seq.d/                       2210   0.0
VFD246 (VFD246Q) /CSM/VF/VFD2-B/VFD246Q.Seq.d/                       2210   0.0
CFC160 (CFC160Q) /CSM/CF/CFC1-C/CFC160Q.Seq.d/                       2210   0.0
SLE328 (SLE328Q) /CSM/SL/SLE3-B/SLE328Q.Seq.d/                       2183   0.0
VFA394 (VFA394Q) /CSM/VF/VFA3-D/VFA394Q.Seq.d/                       2123   0.0
VFG115 (VFG115Q) /CSM/VF/VFG1-A/VFG115Q.Seq.d/                       1292   0.0
VFB745 (VFB745Q) /CSM/VF/VFB7-B/VFB745Q.Seq.d/                       1235   0.0
VFN432 (VFN432Q) /CSM/VF/VFN4-B/VFN432Q.Seq.d/                       1221   0.0
VFN686 (VFN686Q) /CSM/VF/VFN6-D/VFN686Q.Seq.d/                       1207   0.0
AFE566 (AFE566Q) /CSM/AF/AFE5-C/AFE566Q.Seq.d/                       1181   0.0
own update 2001.11.27
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1657  0.0  4
AC116989|AC116989.2 Dictyostelium discoideum chromosome 2 map complement(3527391-3470188) strain AX4, complete sequence.    88  2e-19  8
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36  0.011  8
AX344564|AX344564.1 Sequence 15 from Patent WO0200932.            32  0.069  11
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    34  0.13  10
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32  0.17  9
AC126271|AC126271.4 Mus musculus chromosome 5 clone RP23-237H9, complete sequence.    36  0.28  7
BG893513|BG893513.1 kt10d09.y2 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5', mRNA sequence.    40  0.29  2
AC006281|AC006281.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    30  0.30  13
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    44  0.37  9
dna update 2003. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8R242) RecName: Full=Di-N-acetylchitobiase;          EC=3.2.1....   258   2e-67
BC088895_1(BC088895|pid:none) Xenopus tropicalis hypothetical LO...   257   5e-67
AY260731_1(AY260731|pid:none) Xenopus laevis putative di-N-acety...   249   2e-64
AB168353_1(AB168353|pid:none) Macaca fascicularis testis cDNA cl...   248   2e-64
BC164759_1(BC164759|pid:none) Danio rerio chitobiase, di-N-acety...   244   3e-63
BC095064_1(BC095064|pid:none) Danio rerio chitobiase, di-N-acety...   244   3e-63
AK314640_1(AK314640|pid:none) Homo sapiens cDNA, FLJ95483, highl...   244   4e-63
BC072723_1(BC072723|pid:none) Danio rerio chitobiase, di-N-acety...   243   1e-62
BC022594_1(BC022594|pid:none) Mus musculus chitobiase, di-N-acet...   226   9e-58
AC115592_28(AC115592|pid:none) Dictyostelium discoideum chromoso...   223   1e-56
protein update 2009. 7.10
PSORT 

 psg: 1.01  gvh: 0.77  alm: 0.45  top: 0.33  tms: 0.00  mit: 0.44  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VFD246 is exc
5' end seq. ID VFD246F
5' end seq. 
>VFD246F.Seq
ACCACGATGAGAATTATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCA
TCATCATCATCCTCTTCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAA
ATTGCACCAAGACAAGAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTAT
TATTGGAATCAATTAACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTA
TGTATTGCACATGAAAATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAAT
CTTGGTAATTCAAGTTATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGT
ACATTTACAGATGGTTTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCT
CAACAATATACAGAATTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTC
TATCAAATTTCAATCGATGTAGCATGGTCACCATCATGCATTGATAAACGTTGTTATGAT
TATGCTGGTTTAGCTTCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGT
CAAGTTTTTGGTGAGAAGGTGTGTACTGCTGGTGCA----------
Length of 5' end seq. 636
3' end seq. ID VFD246Z
3' end seq. 
>VFD246Z.Seq
----------AGTCCAATTACAGATCCAACCATCGCTCAACAATATACAGAATTAGTATC
AGCAACTAATAAAGCATTTAAAGCAATTAATCCATTCTATCAAATTTCAATCGATGTAGC
ATGGTCACCATCATGCATTGATAAACGTTGTTATGATTATGCTGGTTTAGCTTCAAATTC
CGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGTCAAGTTTTTGGTGAGAAGGTGTG
TACTGCTGGTGCAAATTCTTCACCCTCAAATGCCTTGGCTGGTATAAATAATTTTACTGA
TTTAGGTATTAGCACTGATCAATTGGTAATGGGTTTACCATGGTATGGTTATATTTATAA
GAATTGTTTAAATGGTGATGAAGCAGGTTTGGAAACTGTTGTTTGTCAAATTGAATCTGT
ACCATTTAGAGGTGCAAATTGTTCAGATGCAGCTGGTTCAGAATATGATTATAGTTATTT
GGTTCAATTATTACAAGATCAAACTATAAACTCTTCTGCAGTTCAATGGAATACTGAATG
GCAATCACCTTATTTTAATTATATTGACCCTATAACTGGTAATGTTGATCAAGTTTGGTT
TGATAATCCACAATCATTATCAATTAAAGTTCAATTAGCTCAAAAATTAAATTTAAGAGG
TGTTGCAGTTTGGAATATTGATTTTTTAGATTTTTCAGATCAATACAATTCTCGTCCAAT
GTGGGATGCATTAGCTTCTTTTTTCCCACAATC
Length of 3' end seq. 743
Connected seq. ID VFD246P
Connected seq. 
>VFD246P.Seq
ACCACGATGAGAATTATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCA
TCATCATCATCCTCTTCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAA
ATTGCACCAAGACAAGAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTAT
TATTGGAATCAATTAACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTA
TGTATTGCACATGAAAATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAAT
CTTGGTAATTCAAGTTATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGT
ACATTTACAGATGGTTTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCT
CAACAATATACAGAATTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTC
TATCAAATTTCAATCGATGTAGCATGGTCACCATCATGCATTGATAAACGTTGTTATGAT
TATGCTGGTTTAGCTTCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGT
CAAGTTTTTGGTGAGAAGGTGTGTACTGCTGGTGCA----------AGTCCAATTACAGA
TCCAACCATCGCTCAACAATATACAGAATTAGTATCAGCAACTAATAAAGCATTTAAAGC
AATTAATCCATTCTATCAAATTTCAATCGATGTAGCATGGTCACCATCATGCATTGATAA
ACGTTGTTATGATTATGCTGGTTTAGCTTCAAATTCCGATTTCCTTGTAGCAATGGACTA
TGATGAGAGAAGTCAAGTTTTTGGTGAGAAGGTGTGTACTGCTGGTGCAAATTCTTCACC
CTCAAATGCCTTGGCTGGTATAAATAATTTTACTGATTTAGGTATTAGCACTGATCAATT
GGTAATGGGTTTACCATGGTATGGTTATATTTATAAGAATTGTTTAAATGGTGATGAAGC
AGGTTTGGAAACTGTTGTTTGTCAAATTGAATCTGTACCATTTAGAGGTGCAAATTGTTC
AGATGCAGCTGGTTCAGAATATGATTATAGTTATTTGGTTCAATTATTACAAGATCAAAC
TATAAACTCTTCTGCAGTTCAATGGAATACTGAATGGCAATCACCTTATTTTAATTATAT
TGACCCTATAACTGGTAATGTTGATCAAGTTTGGTTTGATAATCCACAATCATTATCAAT
TAAAGTTCAATTAGCTCAAAAATTAAATTTAAGAGGTGTTGCAGTTTGGAATATTGATTT
TTTAGATTTTTCAGATCAATACAATTCTCGTCCAATGTGGGATGCATTAGCTTCTTTTTT
CCCACAATC
Length of connected seq. 1379
Full length Seq ID VFD246E
Full length Seq. 
>VFD246E.Seq
ACCACGATGAGAATTATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCA
TCATCATCATCCTCTTCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAA
ATTGCACCAAGACAAGAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTAT
TATTGGAATCAATTAACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTA
TGTATTGCACATGAAAATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAAT
CTTGGTAATTCAAGTTATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGT
ACATTTACAGATGGTTTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCT
CAACAATATACAGAATTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTC
TATCAAATTTCAATCGATGTAGCATGGTCACCATCATGCATTGATAAACGTTGTTATGAT
TATGCTGGTTTAGCTTCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGT
CAAGTTTTTGGTGAGAAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTCAAATGCCTTG
GCTGGTATAAATAATTTTACTGATTTAGGTATTAGCACTGATCAATTGGTAATGGGTTTA
CCATGGTATGGTTATATTTATAAGAATTGTTTAAATGGTGATGAAGCAGGTTTGGAAACT
GTTGTTTGTCAAATTGAATCTGTACCATTTAGAGGTGCAAATTGTTCAGATGCAGCTGGT
TCAGAATATGATTATAGTTATTTGGTTCAATTATTACAAGATCAAACTATAAACTCTTCT
GCAGTTCAATGGAATACTGAATGGCAATCACCTTATTTTAATTATATTGACCCTATAACT
GGTAATGTTGATCAAGTTTGGTTTGATAATCCACAATCATTATCAATTAAAGTTCAATTA
GCTCAAAAATTAAATTTAAGAGGTGTTGCAGTTTGGAATATTGATTTTTTAGATTTTTCA
GATCAATACAATTCTCGTCCAATGTGGGATGCATTAGCTTCTTTTTTCCCACAATC
Length of full length seq. 1136