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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM283
Library AF
(Link to library)
Clone ID AFM283
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM283P
(Link to Original site)
Representative DNA sequence 
>AFM283 (AFM283Q) /CSM/AF/AFM2-D/AFM283Q.Seq.d/
TTGCAATTGTTTCCCAAAATTACACACACCACAAAAATAAAAGAGATAAGAAAAACAGAA
AAAATAAAAAATAAGAAAAACTCTATATAAAATTCTTATTTATATATAAATAAAACAAAA
GTAAAAAAAAGAATGTTTCCCATTTCAGTAGGGTGGATAGCATTTGCAGGTGTTCTTCTC
ACGTTAATTGCATTAAATTATTTTATAGTTAAATATTACTCAGATAAACATGAAAGTGGA
AAAATTACAACAATCATTTCAGTAATTGGACTTACATTAACACTTTTATGTGTTATGCTT
ATTCCAGTTGATATTTTAAATGTTTCAACAATGTCACATAGTGATGGTACACAAATTTCA
CCATATTCAATCTCTCAAAGAACTGAAGGTGTTAAAATGTTATATTATATATTATATGGA
TCAATTTTAGGAGTTTCACTAATTTTAGTACCATTTGCATATTTTTATCATGAAGAATAT
GATGAAAATATTCCAACATGTTCAAGAGTTTATGCAGGATGTAAATTTACAATATTTTTC
ATATTGTTTACAATTATATTATXXXXXXXXXXTCTTTAGACCATTTGCATTCATTTTTGG
TTTCATCTTTATCTTGGTCTCTTTATTGATCATTATCTCCATCGTCTTATCGTTGGTCGA
TAAAATCTCAAGTAGTGTCTGTGGTTCGGCTTGCGGTTTCCTTTCAACTTATCCACAATT
GAAAAATCCATTCGATATCATTCTCACCAAATTGGCCCCATATTTCCCATTGGATTATAT
CATTATTGGTGGTTTAATATTTTTCATTTATTCTTGTACTTTATCTGGTATCACTAGAAT
TGGTATTAGATTTTTATGGATTAATATGTTTGAATTTGGATATCGTAAAACTTTCCCACA
AGGTTTATTACTTGCATCAGTGTTATTAATGTTAAGTAATTTATGTTTAAATATGCAAAT
TGTAAATTTAGCACCAAGATATGTTATGTATGGTGCTCAAGTCTATTTGAATGGTACTTC
AGTTATACCATGTACAATTAATGCACCAACAGATCTTTGTATTATGTCAGAAATTGGTCT
TTTAACTTCACGTATTCAATTAGGTATTTCTTTCTTTGGTATTGTATTTTATTATGGTAC
TTGGGTAATTGTTGCAACTTTTATTTTAGGTTTAGCTGTTTCAATATTTAAAAGAAGACC
ATCTGCGCTTTAAGTTATTC
sequence update 2009. 4. 3
Translated Amino Acid sequence 
lqlfpkithttkikeirktekiknkknsi*NSYLYINKTKVKKRMFPISVGWIAFAGVLL
TLIALNYFIVKYYSDKHESGKITTIISVIGLTLTLLCVMLIPVDILNVSTMSHSDGTQIS
PYSISQRTEGVKMLYYILYGSILGVSLILVPFAYFYHEEYDENIPTCSRVYAGCKFTIFF
ILFTIIL---

---FRPFAFIFGFIFILVSLLIIISIVLSLVDKISSSVCGSACGFLSTYPQLKNPFDIIL
TKLAPYFPLDYIIIGGLIFFIYSCTLSGITRIGIRFLWINMFEFGYRKTFPQGLLLASVL
LMLSNLCLNMQIVNLAPRYVMYGAQVYLNGTSVIPCTINAPTDLCIMSEIGLLTSRIQLG
ISFFGIVFYYGTWVIVATFILGLAVSIFKRRPSAL*vi
Translated Amino Acid sequence (All Frames) 
Frame A:
lqlfpkithttkikeirktekiknkknsi*NSYLYINKTKVKKRMFPISVGWIAFAGVLL
TLIALNYFIVKYYSDKHESGKITTIISVIGLTLTLLCVMLIPVDILNVSTMSHSDGTQIS
PYSISQRTEGVKMLYYILYGSILGVSLILVPFAYFYHEEYDENIPTCSRVYAGCKFTIFF
ILFTIIL---

---sldhlhsflvsslswsly*slspssyrwsiksqvvsvvrlavsfqlihn*kihsisf
spnwphishwiisllvv*yfsfilvlylvslelvldfygliclnldivklshkvyylhqc
y*c*viyv*ickl*i*hqdmlcmvlksi*mvlqlyhvqlmhqqifvlcqklvf*lhvfn*
vflslvlyfimvlg*llqllf*v*lfqylkedhlrfklf

Frame B:
cncfpklhtpqk*kr*ekqkk*kirktlykiliyi*ikqk*kkecfpfq*gg*hlqvffs
r*lh*iil*lnitqinmkveklqqsfq*ldlh*hfyvlclfqlif*mfqqchivmvhkfh
hiqslkelkvlkcyiiyymdqf*efh*f*yhlhifimknmmkifqhvqefmqdvnlqyfs
yclqlyy---

---l*ticihfwfhlylglfidhylhrlivgr*nlk*clwfglrfpfnlstieksiryhs
hqigpifpiglyhywwfnifhlflyfiwyh*nwy*ifmd*yv*iwis*nfptrfitcisv
invk*fmfkyanckfstkicyvwcsslfewyfsytmyn*ctnrslyyvrnwsfnftysir
yfflwycillwylgnccnfyfrfscfni*kkticalsy

Frame C:
aivsqnythhknkrdkknrknkk*eklyikflfiyk*nkskkknvshfsrvdsicrcssh
vnciklfys*illr*t*kwknynnhfsnwtyintfmcyayss*yfkcfnnvt**wytnft
ifnlskn*rc*nvilyiiwinfrsftnfsticifls*ri**kysnmfkslcrm*iynifh
ivynyi---

---FRPFAFIFGFIFILVSLLIIISIVLSLVDKISSSVCGSACGFLSTYPQLKNPFDIIL
TKLAPYFPLDYIIIGGLIFFIYSCTLSGITRIGIRFLWINMFEFGYRKTFPQGLLLASVL
LMLSNLCLNMQIVNLAPRYVMYGAQVYLNGTSVIPCTINAPTDLCIMSEIGLLTSRIQLG
ISFFGIVFYYGTWVIVATFILGLAVSIFKRRPSAL*vi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM283 (AFM283Q) /CSM/AF/AFM2-D/AFM283Q.Seq.d/                       2101   0.0
VFF438 (VFF438Q) /CSM/VF/VFF4-B/VFF438Q.Seq.d/                       1285   0.0
SFJ506 (SFJ506Q) /CSM/SF/SFJ5-A/SFJ506Q.Seq.d/                       1285   0.0
CFF416 (CFF416Q) /CSM/CF/CFF4-A/CFF416Q.Seq.d/                       1285   0.0
CFC519 (CFC519Q) /CSM/CF/CFC5-A/CFC519Q.Seq.d/                       1283   0.0
CHD865 (CHD865Q) /CSM/CH/CHD8-C/CHD865Q.Seq.d/                       1281   0.0
SFH228 (SFH228Q) /CSM/SF/SFH2-B/SFH228Q.Seq.d/                       1277   0.0
CFF842 (CFF842Q) /CSM/CF/CFF8-B/CFF842Q.Seq.d/                       1277   0.0
AFN155 (AFN155Q) /CSM/AF/AFN1-C/AFN155Q.Seq.d/                       1277   0.0
CFI285 (CFI285Q) /CSM/CF/CFI2-D/CFI285Q.Seq.d/                       1275   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ428290) Dictyostelium discoideum cDNA clone:ddv11l10, 3' ...  1285   0.0    1
(BJ402006) Dictyostelium discoideum cDNA clone:dds20k01, 3' ...  1285   0.0    1
(BJ372085) Dictyostelium discoideum cDNA clone:ddc11p04, 3' ...  1285   0.0    1
(BJ346998) Dictyostelium discoideum cDNA clone:dda25e22, 3' ...  1285   0.0    1
(BJ374222) Dictyostelium discoideum cDNA clone:ddc6e05, 3' e...  1283   0.0    1
(BJ376686) Dictyostelium discoideum cDNA clone:ddc29b18, 3' ...  1281   0.0    1
(BJ400900) Dictyostelium discoideum cDNA clone:dds15g08, 3' ...  1277   0.0    1
(BJ372361) Dictyostelium discoideum cDNA clone:ddc12d12, 3' ...  1277   0.0    1
(BJ347628) Dictyostelium discoideum cDNA clone:dda27m13, 3' ...  1277   0.0    1
(BJ375211) Dictyostelium discoideum cDNA clone:ddc17i22, 3' ...  1275   0.0    1
dna update 2009. 4.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KD1) RecName: Full=Probable lysosomal cobalamin transporter;...   287   4e-76
(A7RM45) RecName: Full=Probable lysosomal cobalamin transporter;       94   7e-18
(Q5ZI05) RecName: Full=Probable lysosomal cobalamin transporter;...    87   1e-15
BC044020_1(BC044020|pid:none) Xenopus laevis hypothetical protei...    86   3e-15
(Q7SYR6) RecName: Full=Probable lysosomal cobalamin transporter;...    86   3e-15
(Q5PR61) RecName: Full=Probable lysosomal cobalamin transporter;...    85   6e-15
(Q0VGV9) RecName: Full=Probable lysosomal cobalamin transporter;...    84   7e-15
(Q4R5E3) RecName: Full=Probable lysosomal cobalamin transporter;...    84   1e-14
(Q8K0B2) RecName: Full=Probable lysosomal cobalamin transporter;...    84   1e-14
(Q3SYY9) RecName: Full=Probable lysosomal cobalamin transporter;...    82   5e-14
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM283 is

5' end seq. ID AFM283F
5' end seq. 
>AFM283F.Seq
TTGCAATTGTTTCCCAAAATTACACACACCACAAAAATAAAAGAGATAAGAAAAACAGAA
AAAATAAAAAATAAGAAAAACTCTATATAAAATTCTTATTTATATATAAATAAAACAAAA
GTAAAAAAAAGAATGTTTCCCATTTCAGTAGGGTGGATAGCATTTGCAGGTGTTCTTCTC
ACGTTAATTGCATTAAATTATTTTATAGTTAAATATTACTCAGATAAACATGAAAGTGGA
AAAATTACAACAATCATTTCAGTAATTGGACTTACATTAACACTTTTATGTGTTATGCTT
ATTCCAGTTGATATTTTAAATGTTTCAACAATGTCACATAGTGATGGTACACAAATTTCA
CCATATTCAATCTCTCAAAGAACTGAAGGTGTTAAAATGTTATATTATATATTATATGGA
TCAATTTTAGGAGTTTCACTAATTTTAGTACCATTTGCATATTTTTATCATGAAGAATAT
GATGAAAATATTCCAACATGTTCAAGAGTTTATGCAGGATGTAAATTTACAATATTTTTC
ATATTGTTTACAATTATATTATXXXXXXXXXX
Length of 5' end seq. 572
3' end seq. ID AFM283Z
3' end seq. 
>AFM283Z.Seq
XXXXXXXXXXTCTTTAGACCATTTGCATTCATTTTTGGTTTCATCTTTATCTTGGTCTCT
TTATTGATCATTATCTCCATCGTCTTATCGTTGGTCGATAAAATCTCAAGTAGTGTCTGT
GGTTCGGCTTGCGGTTTCCTTTCAACTTATCCACAATTGAAAAATCCATTCGATATCATT
CTCACCAAATTGGCCCCATATTTCCCATTGGATTATATCATTATTGGTGGTTTAATATTT
TTCATTTATTCTTGTACTTTATCTGGTATCACTAGAATTGGTATTAGATTTTTATGGATT
AATATGTTTGAATTTGGATATCGTAAAACTTTCCCACAAGGTTTATTACTTGCATCAGTG
TTATTAATGTTAAGTAATTTATGTTTAAATATGCAAATTGTAAATTTAGCACCAAGATAT
GTTATGTATGGTGCTCAAGTCTATTTGAATGGTACTTCAGTTATACCATGTACAATTAAT
GCACCAACAGATCTTTGTATTATGTCAGAAATTGGTCTTTTAACTTCACGTATTCAATTA
GGTATTTCTTTCTTTGGTATTGTATTTTATTATGGTACTTGGGTAATTGTTGCAACTTTT
ATTTTAGGTTTAGCTGTTTCAATATTTAAAAGAAGACCATCTGCGCTTTAAGTTATTC
Length of 3' end seq. 658
Connected seq. ID AFM283P
Connected seq. 
>AFM283P.Seq
TTGCAATTGTTTCCCAAAATTACACACACCACAAAAATAAAAGAGATAAGAAAAACAGAA
AAAATAAAAAATAAGAAAAACTCTATATAAAATTCTTATTTATATATAAATAAAACAAAA
GTAAAAAAAAGAATGTTTCCCATTTCAGTAGGGTGGATAGCATTTGCAGGTGTTCTTCTC
ACGTTAATTGCATTAAATTATTTTATAGTTAAATATTACTCAGATAAACATGAAAGTGGA
AAAATTACAACAATCATTTCAGTAATTGGACTTACATTAACACTTTTATGTGTTATGCTT
ATTCCAGTTGATATTTTAAATGTTTCAACAATGTCACATAGTGATGGTACACAAATTTCA
CCATATTCAATCTCTCAAAGAACTGAAGGTGTTAAAATGTTATATTATATATTATATGGA
TCAATTTTAGGAGTTTCACTAATTTTAGTACCATTTGCATATTTTTATCATGAAGAATAT
GATGAAAATATTCCAACATGTTCAAGAGTTTATGCAGGATGTAAATTTACAATATTTTTC
ATATTGTTTACAATTATATTAT----------TCTTTAGACCATTTGCATTCATTTTTGG
TTTCATCTTTATCTTGGTCTCTTTATTGATCATTATCTCCATCGTCTTATCGTTGGTCGA
TAAAATCTCAAGTAGTGTCTGTGGTTCGGCTTGCGGTTTCCTTTCAACTTATCCACAATT
GAAAAATCCATTCGATATCATTCTCACCAAATTGGCCCCATATTTCCCATTGGATTATAT
CATTATTGGTGGTTTAATATTTTTCATTTATTCTTGTACTTTATCTGGTATCACTAGAAT
TGGTATTAGATTTTTATGGATTAATATGTTTGAATTTGGATATCGTAAAACTTTCCCACA
AGGTTTATTACTTGCATCAGTGTTATTAATGTTAAGTAATTTATGTTTAAATATGCAAAT
TGTAAATTTAGCACCAAGATATGTTATGTATGGTGCTCAAGTCTATTTGAATGGTACTTC
AGTTATACCATGTACAATTAATGCACCAACAGATCTTTGTATTATGTCAGAAATTGGTCT
TTTAACTTCACGTATTCAATTAGGTATTTCTTTCTTTGGTATTGTATTTTATTATGGTAC
TTGGGTAATTGTTGCAACTTTTATTTTAGGTTTAGCTGTTTCAATATTTAAAAGAAGACC
ATCTGCGCTTTAAGTTATTC
Length of connected seq. 1210
Full length Seq ID -
Full length Seq. -
Length of full length seq. -