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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD159
Library VF
(Link to library)
Clone ID VFD159
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFD159P
(Link to Original site)
Representative DNA sequence 
>VFD159 (VFD159Q) /CSM/VF/VFD1-C/VFD159Q.Seq.d/
CATTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTAXXXXXXXXXXCTACGTCGCCCTCGACTTTGAAG
CTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACG
GTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCAT
CATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAAT
GTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTA
TGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCA
TGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTA
TCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAAT
CAGGTCCATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
h*FKYIYINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGM
GQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE
APLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYE
GYALPHAIL---

---YVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGI
HETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPE
RKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikk
Translated Amino Acid sequence (All Frames) 
Frame A:
h*FKYIYINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGM
GQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE
APLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYE
GYALPHAIL---

---lrrprl*s*nanccliisprkiiritrrsshhhr**tfplprsplptiilrygicwy
prnhlqlhhem*c*yp*rfir*rrlirwynyvpryc*syeqrincfspinhen*nhcstr
t*ilcldwwiylsftlnfptnvdlqrrirrirsincsqkmflnylinnkk

Frame B:
insniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvw
vkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lk
ph*iqrptekk*pklclkpstpqpctspfkpsypymplvvppvllwiqvmvsptlyqsmk
vmlyhmps---

---YVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGI
HETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPE
RKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikk

Frame C:
liqiyiyksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygryg
skrlicr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*s
pikskgqqrkndpnyv*nlqhpshvrrhssrliliclwsyhrycygfr*wclphctnl*r
lcfttchl---

---tspstlklkckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnllvs
tkppttps*nvmlisvkiytvtssypvvqlcsqvlliv*tkn*ll*phqp*klksllhqn
vntlsglvdls*lhsqlsnkcgspkknttnqvhqlftenvsklfnk**kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO859 (VFO859Q) /CSM/VF/VFO8-C/VFO859Q.Seq.d/                       1076   0.0
VFO709 (VFO709Q) /CSM/VF/VFO7-A/VFO709Q.Seq.d/                       1076   0.0
VFO588 (VFO588Q) /CSM/VF/VFO5-D/VFO588Q.Seq.d/                       1076   0.0
VFN494 (VFN494Q) /CSM/VF/VFN4-D/VFN494Q.Seq.d/                       1076   0.0
VFN217 (VFN217Q) /CSM/VF/VFN2-A/VFN217Q.Seq.d/                       1076   0.0
VFM820 (VFM820Q) /CSM/VF/VFM8-A/VFM820Q.Seq.d/                       1076   0.0
VFM814 (VFM814Q) /CSM/VF/VFM8-A/VFM814Q.Seq.d/                       1076   0.0
VFM723 (VFM723Q) /CSM/VF/VFM7-A/VFM723Q.Seq.d/                       1076   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       1076   0.0
VFM463 (VFM463Q) /CSM/VF/VFM4-C/VFM463Q.Seq.d/                       1076   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1053  0.0  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   987  0.0  4
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        975  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   975  0.0  3
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   944  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   868  0.0  5
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   846  0.0  13
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   805  0.0  6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   767  0.0  3
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     751  0.0  2
dna update 2003. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF368030_1(AF368030|pid:none) Heliothis virescens actin mRNA, co...   350   e-178
(P23344) RecName: Full=Actin-2;  &S07003(S07003)                      323   e-153
AJ132371_1(AJ132371|pid:none) Allogromia sp. mRNA for actin 2.        321   e-150
AY763403_1(AY763403|pid:none) Tretomphalus sp. B123 clone nTRE35...   244   e-119
AY651918_1(AY651918|pid:none) Macrobrachium rosenbergii beta-act...   236   e-113
(Q54HE9) RecName: Full=Putative actin-27;                             249   e-110
(Q54HE7) RecName: Full=Putative actin-28;                             248   e-109
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   365   1e-99
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   365   1e-99
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   363   4e-99
protein update 2009. 6.18
PSORT 

 psg: 0.53  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFD159 is cyt
5' end seq. ID VFD159F
5' end seq. 
>VFD159F.Seq
CATTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTA----------
Length of 5' end seq. 567
3' end seq. ID VFD159Z
3' end seq. 
>VFD159Z.Seq
----------CTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCAT
CAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAAC
GTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTA
TCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTAT
ACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGA
ACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAG
AACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAAC
AAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAAT
GTTTCTAAATTATTTAATAAATAATAAAAAAAA
Length of 3' end seq. 503
Connected seq. ID VFD159P
Connected seq. 
>VFD159P.Seq
CATTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTA----------CTACGTCGCCCTCGACTTTGAAG
CTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACG
GTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCAT
CATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAAT
GTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTA
TGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCA
TGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTA
TCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAAT
CAGGTCCATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAAA
Length of connected seq. 1070
Full length Seq ID -
Full length Seq. -
Length of full length seq. -