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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD121
Library VF
(Link to library)
Clone ID VFD121
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFD121P
(Link to Original site)
Representative DNA sequence 
>VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/
GGATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAG
TACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGXXXXXXXXXXA
AAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCA
ATCGTTGATCCAGAAGCTGTCAAACCANAGGATTGGAATGAAGAAGACGACGGTGAATGG
GAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCA
AATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAA
GACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATCGGTTTCGAATTATGGCAAGTT
AAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAGCTAAAGAT
TTCTNTGAAAAGACCTTTGTTGCCAACCAANAAGCCGAAAAGAAAATGTTTGACGACCTC
GAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCT
GCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTA
GTTAAAACTGANGNCAAAAAAGAAGAAGNCNAAAAATCAACCNAAAAAGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADD---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPXDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEX
AKDFXEKTFVANQXAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTXXKKEEXXKSTXK
Translated Amino Acid sequence (All Frames) 
Frame A:
gfiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikk
mvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkl
ivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstkl
kpin*lintlwsfhqitptkyssitkrskqvtslmi---

---kgnr*prrc*tsrs*rypsfnr*srscqtxgle*rrrr*mgspnhrqsriqr*mesk
enpksriqr*mgtpin**prir*rq*tlpl**srxyrfrimas*iwfhl*qydrxrlcrx
s*rfl*kdlccqpxsrkenv*rprsrqs*xekes*rkiscrksc*krsrrs**rrrrsr*
rrfs*n*xqkrrxxkinxks

Frame B:
dl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rr
w*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*
lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*
nrsinssihfghftr*hlqstrr*qrdpsr*pr**l---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPXDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEX
AKDFXEKTFVANQXAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTXXKKEEXXKSTXK

Frame C:
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADD---

---rksmtqkmlnqpvmtisqlqsliqklsnxrigmkkttvngkpqpspiqntkvngkqr
ksqiqntkvngxth*litqntlktmnstslmisxlsvsnygklnlvpslti*ssptlsxk
lkisxkrpllptxkpkrkclttskppklxrerkltkn*lpkkllkkkpkklmkkkkkslk
ki*lklxxkkkkxknqpkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       2058   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1283   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1283   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1283   0.0
AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       1283   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       1283   0.0
AFJ522 (AFJ522Q) /CSM/AF/AFJ5-A/AFJ522Q.Seq.d/                       1283   0.0
AFG846 (AFG846Q) /CSM/AF/AFG8-B/AFG846Q.Seq.d/                       1283   0.0
AFG840 (AFG840Q) /CSM/AF/AFG8-B/AFG840Q.Seq.d/                       1283   0.0
AFG591 (AFG591Q) /CSM/AF/AFG5-D/AFG591Q.Seq.d/                       1283   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1269  0.0  2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  1e-23  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68  4e-13  2
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    68  4e-09  2
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    68  3e-07  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    68  3e-07  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    68  3e-07  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    68  3e-07  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    68  3e-07  1
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68  3e-07  1
dna update 2003. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   378   e-103
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   236   e-100
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   233   e-99
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   233   1e-99
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   231   3e-99
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   232   6e-99
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   231   8e-99
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   227   1e-98
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   224   5e-98
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   226   9e-98
protein update 2009. 6.18
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum

>> prediction for VFD121 is exc
5' end seq. ID VFD121F
5' end seq. 
>VFD121F.Seq
GGATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAG
TACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTG----------
Length of 5' end seq. 649
3' end seq. ID VFD121Z
3' end seq. 
>VFD121Z.Seq
----------AAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATA
TCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCANAGGATTGGAATGAAGAAGACG
ACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAA
AGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAG
AATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATCGGTTTCGAAT
TATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAN
AAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAANAAGCCGAAAAGAAAATGT
TTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTG
AAGAAGATTTAGTTAAAACTGANGNCAAAAAAGAAGAAGNCNAAAAATCAACCNAAAAAG
T
Length of 3' end seq. 591
Connected seq. ID VFD121P
Connected seq. 
>VFD121P.Seq
GGATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAG
TACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTG----------A
AAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCA
ATCGTTGATCCAGAAGCTGTCAAACCANAGGATTGGAATGAAGAAGACGACGGTGAATGG
GAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCA
AATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAA
GACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATCGGTTTCGAATTATGGCAAGTT
AAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAGCTAAAGAT
TTCTNTGAAAAGACCTTTGTTGCCAACCAANAAGCCGAAAAGAAAATGTTTGACGACCTC
GAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCT
GCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTA
GTTAAAACTGANGNCAAAAAAGAAGAAGNCNAAAAATCAACCNAAAAAGT
Length of connected seq. 1240
Full length Seq ID -
Full length Seq. -
Length of full length seq. -