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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC660
Library VF
(Link to library)
Clone ID VFC660
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig -
Original site URL
Representative seq. ID VFC660P
(Link to Original site)
Representative DNA sequence 
>VFC660 (VFC660Q) /CSM/VF/VFC6-C/VFC660Q.Seq.d/
AGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGGTGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGG
GCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCATGGTGTCATCAACATGTAA
TTGGTCXXXXXXXXXXACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAG
TGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTA
CCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACAT
TAAACCAAACTTCACTGAAGCTATTATGTCACACATTAAGTTACCTTTACAAAATGGGTA
ATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAATAACTTA
AAAACCAATTATTTACTTTNAACAAACAGTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVGGKVYDITRWVPLHPGGKEV
LLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRKH
FKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHM
MHDSCHAAISHYPGVWKWMGASFDFVTGASFLSWCHQHVIG---

---TQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKP
NFTEAIMSHIKLPLQNG**srlc*ktislkr*lneit*kpiiyfxqtv
Translated Amino Acid sequence (All Frames) 
Frame A:
sifctyinrivikcqk*lqesntlgqs*pnitlkmivglqsvvkfmilqdgyhyiqvvkk
fyf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*krelen
ilkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswqsfthfathysqci
*cmihvmlpfhiiqaygngwahhlisslvlhsfhgvinm*lv---

---TQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKP
NFTEAIMSHIKLPLQNG**srlc*ktislkr*lneit*kpiiyfxqtv

Frame B:
vfsvhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*rs
ftfssr*rcykfi*klssnvr*tninfkel*nwiyfii*tskicskirflqdikres*kt
f*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgnrlrtlqliilnay
da*fmscchftlsrrmemdgrii*frhwcfipfmvsstcnw---

---lkimvmvhsfvpflvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnq
tslkllchtlsylykmgndpdyvkkplaskdd*mk*lknqlftxnkq

Frame C:
yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVGGKVYDITRWVPLHPGGKEV
LLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRKH
FKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHM
MHDSCHAAISHYPGVWKWMGASFDFVTGASFLSWCHQHVIG---

---srlwswftplylf*wffkssscssfipincsrflpttctnckrsl*rt*hylph*tk
lh*syyvth*vtftkwvmiqimlknh*pqkmik*nnlktnyllxtns
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC660 (VFC660Q) /CSM/VF/VFC6-C/VFC660Q.Seq.d/                       1899   0.0
VFL212 (VFL212Q) /CSM/VF/VFL2-A/VFL212Q.Seq.d/                       1312   0.0
VFK616 (VFK616Q) /CSM/VF/VFK6-A/VFK616Q.Seq.d/                       1312   0.0
VFF662 (VFF662Q) /CSM/VF/VFF6-C/VFF662Q.Seq.d/                       1312   0.0
VFD240 (VFD240Q) /CSM/VF/VFD2-B/VFD240Q.Seq.d/                       1312   0.0
VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       1312   0.0
VFA320 (VFA320Q) /CSM/VF/VFA3-A/VFA320Q.Seq.d/                       1312   0.0
VFA219 (VFA219Q) /CSM/VF/VFA2-A/VFA219Q.Seq.d/                       1312   0.0
CFH569 (CFH569Q) /CSM/CF/CFH5-C/CFH569Q.Seq.d/                       1306   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1263   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB029311|AB029311.1 Dictyostelium discoideum FadA gene for fatty acid desaturase, complete cds.  1033  0.0  5
BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.   402  e-162  2
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.   402  e-162  2
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.   402  e-162  2
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.   145  5e-94  8
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34  0.052  15
AL355612|AL355612.8 Human DNA sequence from clone RP11-343P23 on chromosome 6 Contains  GSSs.    42  0.077  3
AC117128|AC117128.8 Rattus norvegicus clone CH230-230O8, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48  0.21  1
AC013843|AC013843.1 Drosophila melanogaster, WORKING DRAFT SEQUENCE, in ordered pieces.    38  0.29  4
AC106720|AC106720.8 Homo sapiens 3 BAC RP11-26M20 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    42  0.34  3
dna update 2003. 9.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   414   e-114
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   278   3e-73
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   267   4e-70
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   160   8e-38
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   152   2e-35
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   150   8e-35
AM949596_1(AM949596|pid:none) Mantoniella squamata d5 gene for A...   121   4e-26
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    120   5e-26
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   117   6e-25
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   108   3e-22
protein update 2009. 6.18
PSORT 

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFC660 is nuc
5' end seq. ID VFC660F
5' end seq. 
>VFC660F.Seq
AGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGGTGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGG
GCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCATGGTGTCATCAACATGTAA
TTGGTC----------
Length of 5' end seq. 666
3' end seq. ID VFC660Z
3' end seq. 
>VFC660Z.Seq
----------ACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTC
TTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACA
ACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACC
AAACTTCACTGAAGCTATTATGTCACACATTAAGTTACCTTTACAAAATGGGTAATGATC
CAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAATAACTTAAAAACC
AATTATTTACTTTNAACAAACAGTA
Length of 3' end seq. 315
Connected seq. ID VFC660P
Connected seq. 
>VFC660P.Seq
AGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGGTGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGG
GCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCATGGTGTCATCAACATGTAA
TTGGTC----------ACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAG
TGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTA
CCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACAT
TAAACCAAACTTCACTGAAGCTATTATGTCACACATTAAGTTACCTTTACAAAATGGGTA
ATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAATAACTTA
AAAACCAATTATTTACTTTNAACAAACAGTA
Length of connected seq. 981
Full length Seq ID -
Full length Seq. -
Length of full length seq. -