NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC605
Library VF
(Link to library)
Clone ID VFC605
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U02087-1|Contig-U16097-1
Original site URL
Representative seq. ID VFC605P
(Link to Original site)
Representative DNA sequence 
>VFC605 (VFC605Q) /CSM/VF/VFC6-A/VFC605Q.Seq.d/
AAACACACTCCACCTGTATTATATCTTTTTTTCTATAAATATATATATATTTTTTTTTAA
AAATAATAAAAAATAATAACAACTCATTTGCATCATCGCTTTTTGTAAACTAGAGGGAAG
AAAAAAAATATAAATAAAAAAAAAAXXXXXXXXXXTAAAAAATCATTCACAGAGAAAGAA
GCAAAATTAATTACTCAACAATTACTCCAATCATTAGTTTATTTACATTCAATTGGTATT
GTTCATAGAGATTTAAAACCAGAGAATTTATTATTAAAAACACCAACCGATTTAACTGTT
GCACTTTCAGATTTTGGTCTCTCCAAAATTGTCGGGGATGACGTTTTTATGAAAACTACT
TGTGGCACTCCATCCTACGTTGCACCAGAAGTATTAAATAATATTTCAAATTCTCCAACA
GCCTATAGTGATGCTGTTGATATGTGGGGTATTGGTGTTATTACTTATATTTTATTATGT
GGTTTTCCACCATTTTATAGTGAAGATATTAGAAAATTATTTGAATCAATTTTATCAGCA
AGTTATGATTTTCCAAATGATTATTGGGGTAATGTTAGTAAAGAAGCTAAACATTTCATT
AATTGTTTANTCNCAGTTGAACCAACANANAGATATTC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
khtppvlylffykyiyiff*k**kiitthlhhrfl*TRGKKKNINKKK---

---KKSFTEKEAKLITQQLLQSLVYLHSIGIVHRDLKPENLLLKTPTDLTVALSDFGLSK
IVGDDVFMKTTCGTPSYVAPEVLNNISNSPTAYSDAVDMWGIGVITYILLCGFPPFYSED
IRKLFESILSASYDFPNDYWGNVSKEAKHFINCLXXVEPTXRY
Translated Amino Acid sequence (All Frames) 
Frame A:
khtppvlylffykyiyiff*k**kiitthlhhrfl*TRGKKKNINKKK---

---*kiihrerskinystitpiislftfnwycs*rfktrefiikntnrfncctfrfwslq
ncrg*rfyenylwhsilrctrsik*yfkfsnsl**cc*yvgywcyylyfimwfstil**r
y*kii*infiskl*fsk*llg*c**rs*tfh*lfxxs*tnxxif

Frame B:
ntlhlyyiffsiniyifffknnkk**qliciiafcklegrkki*ikkk---

---KKSFTEKEAKLITQQLLQSLVYLHSIGIVHRDLKPENLLLKTPTDLTVALSDFGLSK
IVGDDVFMKTTCGTPSYVAPEVLNNISNSPTAYSDAVDMWGIGVITYILLCGFPPFYSED
IRKLFESILSASYDFPNDYWGNVSKEAKHFINCLXXVEPTXRY

Frame C:
thstciisffl*iyiyfflkiiknnnnsfasslfvn*reekkyk*kk---

---knhsqrkkqn*llnnysnh*fiyiqlvlfiei*nqriyy*khqpi*llhfqilvspk
lsgmtfl*kllvalhptlhqky*iifqilqqpivmllicgvlvlllifyyvvfhhfivki
lenylnqfyqqvmifqmiigvmlvkklnislivxsqlnqxxdi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC605 (VFC605Q) /CSM/VF/VFC6-A/VFC605Q.Seq.d/                        934   0.0
VFK646 (VFK646Q) /CSM/VF/VFK6-B/VFK646Q.Seq.d/                        894   0.0
VSF220 (VSF220Q) /CSM/VS/VSF2-A/VSF220Q.Seq.d/                         86   8e-16
VSE849 (VSE849Q) /CSM/VS/VSE8-C/VSE849Q.Seq.d/                         86   8e-16
VSE625 (VSE625Q) /CSM/VS/VSE6-B/VSE625Q.Seq.d/                         86   8e-16
VSE601 (VSE601Q) /CSM/VS/VSE6-A/VSE601Q.Seq.d/                         86   8e-16
SSM378 (SSM378Q) /CSM/SS/SSM3-D/SSM378Q.Seq.d/                         86   8e-16
SSJ247 (SSJ247Q) /CSM/SS/SSJ2-B/SSJ247Q.Seq.d/                         86   8e-16
SSG783 (SSG783Q) /CSM/SS/SSG7-D/SSG783Q.Seq.d/                         86   8e-16
SLI519 (SLI519Q) /CSM/SL/SLI5-A/SLI519Q.Seq.d/                         86   8e-16
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AZ667950|AZ667950.1 ENTFN88TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    70  2e-19  4
AB118103|AB118103.1 Entamoeba histolytica gene for hypothetical protein, complete cds, clone:Eh-2109.    70  2e-19  4
M64176|M64176.1 D.discoideum myosin light chain kinase (MLCK) mRNA, complete cds.    86  5e-19  3
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    64  4e-16  9
AZ930615|AZ930615.1 474.dhz56f04.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz56f04.s1, DNA sequence.    56  1e-08  2
AC115685|AC115685.1 Dictyostelium discoideum chromosome 2 map 4718821-4752388 strain AX4, complete sequence.    70  2e-07  3
AL402353|AL402353.1 T7 end of clone AT0AA001D11 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    48  3e-07  2
BP190282|BP190282.1 Dugesia japonica cDNA, clone: 04589_HH, expressed in the head.    66  6e-07  1
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    70  8e-07  9
I38342|I38342.1 Sequence 1 from patent US 5614195.                64  2e-06  1
dna update 2003. 9.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54CY9) RecName: Full=Probable myosin light chain kinase DDB_G0...   320   2e-86
(P25323) RecName: Full=Myosin light chain kinase A;          Sho...   185   8e-46
A40811(A40811;A37125)myosin-light-chain kinase (EC 2.7.1.117) A ...   184   2e-45
BT045374_1(BT045374|pid:none) Salmo salar clone ssal-rgf-519-258...   170   3e-41
(Q869W6) RecName: Full=Probable myosin light chain kinase DDB_G0...   169   5e-41
AY606040_1(AY606040|pid:none) Oncorhynchus mykiss calcium/calmod...   169   5e-41
BC041721_1(BC041721|pid:none) Xenopus laevis calcium/calmodulin-...   167   2e-40
BC110966_1(BC110966|pid:none) Xenopus laevis calcium/calmodulin-...   167   2e-40
FN357490_32(FN357490|pid:none) Schistosoma mansoni genome sequen...   167   2e-40
AK052401_1(AK052401|pid:none) Mus musculus 13 days embryo lung c...   167   2e-40
protein update 2009. 6.18
PSORT 

 psg: 0.36  gvh: 0.45  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.02
 nuc: 0.08  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal

>> prediction for VFC605 is cyt
5' end seq. ID VFC605F
5' end seq. 
>VFC605F.Seq
AAACACACTCCACCTGTATTATATCTTTTTTTCTATAAATATATATATATTTTTTTTTAA
AAATAATAAAAAATAATAACAACTCATTTGCATCATCGCTTTTTGTAAACTAGAGGGAAG
AAAAAAAATATAAATAAAAAAAAAA----------
Length of 5' end seq. 145
3' end seq. ID VFC605Z
3' end seq. 
>VFC605Z.Seq
----------TAAAAAATCATTCACAGAGAAAGAAGCAAAATTAATTACTCAACAATTAC
TCCAATCATTAGTTTATTTACATTCAATTGGTATTGTTCATAGAGATTTAAAACCAGAGA
ATTTATTATTAAAAACACCAACCGATTTAACTGTTGCACTTTCAGATTTTGGTCTCTCCA
AAATTGTCGGGGATGACGTTTTTATGAAAACTACTTGTGGCACTCCATCCTACGTTGCAC
CAGAAGTATTAAATAATATTTCAAATTCTCCAACAGCCTATAGTGATGCTGTTGATATGT
GGGGTATTGGTGTTATTACTTATATTTTATTATGTGGTTTTCCACCATTTTATAGTGAAG
ATATTAGAAAATTATTTGAATCAATTTTATCAGCAAGTTATGATTTTCCAAATGATTATT
GGGGTAATGTTAGTAAAGAAGCTAAACATTTCATTAATTGTTTANTCNCAGTTGAACCAA
CANANAGATATTC
Length of 3' end seq. 483
Connected seq. ID VFC605P
Connected seq. 
>VFC605P.Seq
AAACACACTCCACCTGTATTATATCTTTTTTTCTATAAATATATATATATTTTTTTTTAA
AAATAATAAAAAATAATAACAACTCATTTGCATCATCGCTTTTTGTAAACTAGAGGGAAG
AAAAAAAATATAAATAAAAAAAAAA----------TAAAAAATCATTCACAGAGAAAGAA
GCAAAATTAATTACTCAACAATTACTCCAATCATTAGTTTATTTACATTCAATTGGTATT
GTTCATAGAGATTTAAAACCAGAGAATTTATTATTAAAAACACCAACCGATTTAACTGTT
GCACTTTCAGATTTTGGTCTCTCCAAAATTGTCGGGGATGACGTTTTTATGAAAACTACT
TGTGGCACTCCATCCTACGTTGCACCAGAAGTATTAAATAATATTTCAAATTCTCCAACA
GCCTATAGTGATGCTGTTGATATGTGGGGTATTGGTGTTATTACTTATATTTTATTATGT
GGTTTTCCACCATTTTATAGTGAAGATATTAGAAAATTATTTGAATCAATTTTATCAGCA
AGTTATGATTTTCCAAATGATTATTGGGGTAATGTTAGTAAAGAAGCTAAACATTTCATT
AATTGTTTANTCNCAGTTGAACCAACANANAGATATTC
Length of connected seq. 628
Full length Seq ID -
Full length Seq. -
Length of full length seq. -