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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC590
Library VF
(Link to library)
Clone ID VFC590
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13954-1
Original site URL
Representative seq. ID VFC590E
(Link to Original site)
Representative DNA sequence 
>VFC590 (VFC590Q) /CSM/VF/VFC5-D/VFC590Q.Seq.d/
TATTTTTCTTTACACAATAAAAAATTTTTCAAATAAATAAATAAAATGGTTGTCCCAATT
GGTATTAATGGTTTTGGTCGTATCGGTCGTCTTGTATTAAGAGCTTCATTAGAAAACCCA
GAATGTAGAGTTTTAGCCATCAATGAACCATTCATGGATGTCAAATATATGGTATATATG
TTCAAATATGATTCAACCCACGGTCGTTTCAAAGGTACCGTCGAAGATATTAATGGTGAA
TTTGTTGTAAATGGTAACAAAATCCACGTTTTCGCTGAAAAAGACCCAGCCAATATTAAA
TGGTCTTCTGTTGGTGCTGAATACATTGTAGAATCAACTGGTCTCTTTTTATCAACTGAA
AAGGCTGGTGTTCACTTAAAAGGTGGTGCCAAAAAAAGTTGTTATCTCTGCTCCATCAAC
TGATGCCCCAATGTACGTTATGGGTGTCAATGAAGAAACCTATGAATCATCACACGACGT
CATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAGCTAAAATTATCCACGA
AAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCATCACTGCCACCCAAAA
GACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGATCTGCCCTTTCAAACAT
TATCCCAGCCTCAACTGGTGCCGCCAAAGCTGTCGGTAAAGTTTTACCAGCACTCAATGG
TAAATTAACCGGTATGTCATTCCGTGTTCCAAATTGTGATGTTTCAGTCGTCGATTTAAC
CGTCCGTCTTGAAAAGAAAGCCACCTATGAAGAAATCAAAAAGGTCATGAAAGCTGCCTC
TGAATCTGACAAATACAAGAGATACATTGGTTACACTGAAGATGAAGTTGTCTCAACTGA
CTTTATTGGTGATACTCACTCTTCAATTTTCGATGCTCATGCTGGTATCGCCTTAAATGA
TAACTTTGTTAAATTAGTCTCATGGTATGATAATGAAATGGGTTATTCAACTCGTGTTAT
TGATTTACTTGTTTATATCTCAAAAAAGAATTAAATAAAAAAAATAAATAAAATAAAAAC
AAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
iflytiknfsnk*ikwlsqlvlmvlvvsvvly*elh*ktqnvef*psmnhswmsniwyic
snmiqptvvskvpskilmvnll*mvtkstfslkktqpilnglllvlntl*nqlvsfyqlk
rlvft*KVVPKKVVISAPSTDAPMYVMGVNEETYESSHDVISNASCTTNCLAPLAKIIHE
NFGIVEGLMTTVHAITATQKTVDGPSGKDWRAGRSALSNIIPASTGAAKAVGKVLPALNG
KLTGMSFRVPNCDVSVVDLTVRLEKKATYEEIKKVMKAASESDKYKRYIGYTEDEVVSTD
FIGDTHSSIFDAHAGIALNDNFVKLVSWYDNEMGYSTRVIDLLVYISKKN*ikkinkikt
Translated Amino Acid sequence (All Frames) 
Frame A:
yfslhnkkffk*inkmvvpigingfgrigrlvlraslenpecrvlainepfmdvkymvym
fkydsthgrfkgtvedingefvvngnkihvfaekdpanikwssvgaeyivestglflste
kagvhlkggakkscylcsin*cpnvrygcq*rnl*iitrrhfkcimyhqlfssis*nypr
klwyc*rfndhrsrhhchpkdc*wsiw*rlesw*icpfkhypslnwcrqscr*sftstqw
*inryvipcskl*cfsrrfnrps*keshl*rnqkghescl*i*qiqeihwlh*r*scln*
lyw*yslfnfrcscwyrlk**lc*islmv***nglfnscy*ftclylkkelnkknk*nkn
k


Frame B:
iflytiknfsnk*ikwlsqlvlmvlvvsvvly*elh*ktqnvef*psmnhswmsniwyic
snmiqptvvskvpskilmvnll*mvtkstfslkktqpilnglllvlntl*nqlvsfyqlk
rlvft*KVVPKKVVISAPSTDAPMYVMGVNEETYESSHDVISNASCTTNCLAPLAKIIHE
NFGIVEGLMTTVHAITATQKTVDGPSGKDWRAGRSALSNIIPASTGAAKAVGKVLPALNG
KLTGMSFRVPNCDVSVVDLTVRLEKKATYEEIKKVMKAASESDKYKRYIGYTEDEVVSTD
FIGDTHSSIFDAHAGIALNDNFVKLVSWYDNEMGYSTRVIDLLVYISKKN*ikkinkikt


Frame C:
ffftq*kifqink*ngcpnwy*wfwsyrssciksfirkprm*sfshq*tihgcqiygiyv
qi*fnprsfqryrrry*w*icckw*qnprfr*krpsqy*mvfcwc*ihcrinwslfin*k
gwcslkrwcqkkllsllhqlmpqctlwvsmkkpmnhhttsfqmhhvppiv*lh*lklstk
tlvllkv**ppftpslppkrllmvhlvkigelvdlpfqtlsqpqlvppklsvkfyqhsmv
n*pvchsvfqivmfqssi*psvlkrkppmkkskrs*klplnltntrdtlvtlkmklsqlt
llviltlqfsmlmlvsp*mitlln*shgmimkwviqlvlliylfisqkrik*kk*ik*kq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC590 (VFC590Q) /CSM/VF/VFC5-D/VFC590Q.Seq.d/                       1929   0.0
SFJ752 (SFJ752Q) /CSM/SF/SFJ7-C/SFJ752Q.Seq.d/                       1919   0.0
SFH716 (SFH716Q) /CSM/SF/SFH7-A/SFH716Q.Seq.d/                       1919   0.0
SFE790 (SFE790Q) /CSM/SF/SFE7-D/SFE790Q.Seq.d/                       1919   0.0
SFD103 (SFD103Q) /CSM/SF/SFD1-A/SFD103Q.Seq.d/                       1919   0.0
CHD590 (CHD590Q) /CSM/CH/CHD5-D/CHD590Q.Seq.d/                       1919   0.0
CFC682 (CFC682Q) /CSM/CF/CFC6-D/CFC682Q.Seq.d/                       1919   0.0
VFK758 (VFK758Q) /CSM/VF/VFK7-C/VFK758Q.Seq.d/                       1911   0.0
SFJ836 (SFJ836Q) /CSM/SF/SFJ8-B/SFJ836Q.Seq.d/                       1911   0.0
SFJ724 (SFJ724Q) /CSM/SF/SFJ7-A/SFJ724Q.Seq.d/                       1911   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1277  0.0  5
U55243|U55243.1 Dictyostelium discoideum glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, partial cds.  1160  0.0  3
AF479765|AF479765.1 Plasmodium falciparum HB3 glyceraldehyde-3-phosphate dehydrogenase mRNA, complete cds.    78  7e-50  7
AF348158|AF348158.1 Plasmodium falciparum strain FCC-1/HN glyceraldehyde-3-phosphate dehydrogenase mRNA, complete cds.    78  1e-49  7
AF030440|AF030440.1 Plasmodium falciparum glyceraldehyde-3-phosphate dehydrogenase mRNA, complete cds.    78  3e-49  7
BD218177|BD218177.1 Plasmid for gene expression in pichia ciferri and transformation method using the same.   137  2e-48  6
AF053300|AF053300.1 Pichia ciferrii glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene, complete cds.   137  2e-48  6
BI816321|BI816321.1 PfESToaa39a10.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:O96369 O96369 GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ;, mRNA sequence.    78  3e-42  5
BI815601|BI815601.1 PfESToaa30e06.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:O96369 O96369 GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ;, mRNA sequence.    78  3e-42  5
AL402531|AL402531.1 T7 end of clone AT0AA002F10 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    92  2e-41  7
dna update 2003. 9.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94469) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   424   0.0
U55243_1(U55243|pid:none) Dictyostelium discoideum glyceraldehyd...   383   e-162
(Q8NK47) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   334   e-133
(Q9Y8E9) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   324   e-132
(Q96UF2) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase ...   323   e-130
(P17244) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   324   e-130
(A3FKF7) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   325   e-129
(Q28259) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   323   e-129
(Q4KYY3) RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;...   323   e-129
AB070217_1(AB070217|pid:none) Meriones unguiculatus GAPD mRNA fo...   322   e-129
protein update 2009. 7.10
PSORT 

 psg: 0.88  gvh: 0.41  alm: 0.43  top: 0.00  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: extracellular, including cell wall
	 24.0 %: cytoplasmic
	 16.0 %: vacuolar
	 12.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFC590 is exc
5' end seq. ID VFC590F
5' end seq. 
>VFC590F.Seq
TATTTTTCTTTACACAATAAAAAATTTTTCAAATAAATAAATAAAATGGTTGTCCCAATT
GGTATTAATGGTTTTGGTCGTATCGGTCGTCTTGTATTAAGAGCTTCATTAGAAAACCCA
GAATGTAGAGTTTTAGCCATCAATGAACCATTCATGGATGTCAAATATATGGTATATATG
TTCAAATATGATTCAACCCACGGTCGTTTCAAAGGTACCGTCGAAGATATTAATGGTGAA
TTTGTTGTAAATGGTAACAAAATCCACGTTTTCGCTGAAAAAGACCCAGCCAATATTAAA
TGGTCTTCTGTTGGTGCTGAATACATTGTAGAATCAACTGGTCTCTTTTTATCAACTGAA
AAGGCTGGTGTTCACTTAAAAGGTGGTGCCAAAAAAAGTTGTTATCTCTGCTCCATCAAC
TGATGCCCCAATGTACGTTATGGGTGTCAATGAAGAAACCTATGAATCATCACACGACGT
CATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAGCTAAAATTATCCACGA
AAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCATCACTGCCACCCAAAA
GACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGATCTGCCCTTTC------
----
Length of 5' end seq. 654
3' end seq. ID VFC590Z
3' end seq. 
>VFC590Z.Seq
----------ACTGAAAAGGCTGGTGTTCACTTAAAAGGTGGTGCCAAAAAAAGTTGTTA
TCTCTGCTCCATCAACTGATGCCCCAATGTACGTTATGGGTGTCAATGAAGAAACCTATG
AATCATCACACGACGTCATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAG
CTAAAATTATCCACGAAAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCA
TCACTGCCACCCAAAAGACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGAT
CTGCCCTTTCAAACATTATCCCAGCCTCAACTGGTGCCGCCAAAGCTGTCGGTAAAGTTT
TACCAGCACTCAATGGTAAATTAACCGGTATGTCATTCCGTGTTCCAAATTGTGATGTTT
CAGTCGTCGATTTAACCGTCCGTCTTGAAAAGAAAGCCACCTATGAAGAAATCAAAAAGG
TCATGAAAGCTGCCTCTGAATCTGACAAATACAAGAGATACATTGGTTACACTGAAGATG
AAGTTGTCTCAACTGACTTTATTGGTGATACTCACTCTTCAATTTTCGATGCTCATGCTG
GTATCGCCTTAAATGATAACTTTGTTAAATTAGTCTCATGGTATGATAATGAAATGGGTT
ATTCAACTCGTGTTATTGATTTACTTGTTTATATCTCAAAAAAGAATTAAATAAAAAAAA
TAAATAAAATAAAAACAAA
Length of 3' end seq. 729
Connected seq. ID VFC590P
Connected seq. 
>VFC590P.Seq
TATTTTTCTTTACACAATAAAAAATTTTTCAAATAAATAAATAAAATGGTTGTCCCAATT
GGTATTAATGGTTTTGGTCGTATCGGTCGTCTTGTATTAAGAGCTTCATTAGAAAACCCA
GAATGTAGAGTTTTAGCCATCAATGAACCATTCATGGATGTCAAATATATGGTATATATG
TTCAAATATGATTCAACCCACGGTCGTTTCAAAGGTACCGTCGAAGATATTAATGGTGAA
TTTGTTGTAAATGGTAACAAAATCCACGTTTTCGCTGAAAAAGACCCAGCCAATATTAAA
TGGTCTTCTGTTGGTGCTGAATACATTGTAGAATCAACTGGTCTCTTTTTATCAACTGAA
AAGGCTGGTGTTCACTTAAAAGGTGGTGCCAAAAAAAGTTGTTATCTCTGCTCCATCAAC
TGATGCCCCAATGTACGTTATGGGTGTCAATGAAGAAACCTATGAATCATCACACGACGT
CATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAGCTAAAATTATCCACGA
AAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCATCACTGCCACCCAAAA
GACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGATCTGCCCTTTC------
----ACTGAAAAGGCTGGTGTTCACTTAAAAGGTGGTGCCAAAAAAAGTTGTTATCTCTG
CTCCATCAACTGATGCCCCAATGTACGTTATGGGTGTCAATGAAGAAACCTATGAATCAT
CACACGACGTCATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAGCTAAAA
TTATCCACGAAAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCATCACTG
CCACCCAAAAGACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGATCTGCCC
TTTCAAACATTATCCCAGCCTCAACTGGTGCCGCCAAAGCTGTCGGTAAAGTTTTACCAG
CACTCAATGGTAAATTAACCGGTATGTCATTCCGTGTTCCAAATTGTGATGTTTCAGTCG
TCGATTTAACCGTCCGTCTTGAAAAGAAAGCCACCTATGAAGAAATCAAAAAGGTCATGA
AAGCTGCCTCTGAATCTGACAAATACAAGAGATACATTGGTTACACTGAAGATGAAGTTG
TCTCAACTGACTTTATTGGTGATACTCACTCTTCAATTTTCGATGCTCATGCTGGTATCG
CCTTAAATGATAACTTTGTTAAATTAGTCTCATGGTATGATAATGAAATGGGTTATTCAA
CTCGTGTTATTGATTTACTTGTTTATATCTCAAAAAAGAATTAAATAAAAAAAATAAATA
AAATAAAAACAAA
Length of connected seq. 1383
Full length Seq ID VFC590E
Full length Seq. 
>VFC590E.Seq
TATTTTTCTTTACACAATAAAAAATTTTTCAAATAAATAAATAAAATGGTTGTCCCAATT
GGTATTAATGGTTTTGGTCGTATCGGTCGTCTTGTATTAAGAGCTTCATTAGAAAACCCA
GAATGTAGAGTTTTAGCCATCAATGAACCATTCATGGATGTCAAATATATGGTATATATG
TTCAAATATGATTCAACCCACGGTCGTTTCAAAGGTACCGTCGAAGATATTAATGGTGAA
TTTGTTGTAAATGGTAACAAAATCCACGTTTTCGCTGAAAAAGACCCAGCCAATATTAAA
TGGTCTTCTGTTGGTGCTGAATACATTGTAGAATCAACTGGTCTCTTTTTATCAACTGAA
AAGGCTGGTGTTCACTTAAAAGGTGGTGCCAAAAAAAGTTGTTATCTCTGCTCCATCAAC
TGATGCCCCAATGTACGTTATGGGTGTCAATGAAGAAACCTATGAATCATCACACGACGT
CATTTCAAATGCATCATGTACCACCAATTGTTTAGCTCCATTAGCTAAAATTATCCACGA
AAACTTTGGTATTGTTGAAGGTTTAATGACCACCGTTCACGCCATCACTGCCACCCAAAA
GACTGTTGATGGTCCATCTGGTAAAGATTGGAGAGCTGGTAGATCTGCCCTTTCAAACAT
TATCCCAGCCTCAACTGGTGCCGCCAAAGCTGTCGGTAAAGTTTTACCAGCACTCAATGG
TAAATTAACCGGTATGTCATTCCGTGTTCCAAATTGTGATGTTTCAGTCGTCGATTTAAC
CGTCCGTCTTGAAAAGAAAGCCACCTATGAAGAAATCAAAAAGGTCATGAAAGCTGCCTC
TGAATCTGACAAATACAAGAGATACATTGGTTACACTGAAGATGAAGTTGTCTCAACTGA
CTTTATTGGTGATACTCACTCTTCAATTTTCGATGCTCATGCTGGTATCGCCTTAAATGA
TAACTTTGTTAAATTAGTCTCATGGTATGATAATGAAATGGGTTATTCAACTCGTGTTAT
TGATTTACTTGTTTATATCTCAAAAAAGAATTAAATAAAAAAAATAAATAAAATAAAAAC
AAA
Length of full length seq. 1083