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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC481
Library VF
(Link to library)
Clone ID VFC481
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16285-1
Original site URL
Representative seq. ID VFC481P
(Link to Original site)
Representative DNA sequence 
>VFC481 (VFC481Q) /CSM/VF/VFC4-D/VFC481Q.Seq.d/
ACTGTTGGCCTACTGGNATTACATAGTTTTTATAAAAAAGGAAAAAAATGAGATTCATCT
TATTCTTTGTTTTAATGTTAACAGCCTTGGCTGCTGGTAGAAGATTATCAGTTGAGGAAA
GTCAATTCATTGCCTTCCAAAATAAATATAATAAAATTTATTCAGCTGAAGAATATTTAG
TTAAATTTGAAACCTTCAAATCAAATTTATTAAATATTGATGCCTTAAACAAACAAGCCA
CCACCATTGGATCTGATACTAAATTTGGTGTCAACAAATTTGCTGATCTCTCAAAAGAAG
AATTCAAAAAATATTACTTAAGCAGCAAAGAAGCCCGTTTAACTGATGACCTCCCAXXXX
XXXXXXCCTATCCATACACTGCTGTTGATGGAGAATGTAAATTTAACTCTGCCCAAGTTG
GTGCTAAAATTTCATCTTTCACTATGGTTCCACAAAATGAAACTCAAATTGCTTCCTACT
TATTCAACAATGGTCCATTAGCTATTGCAGCTGATGCTGAAGAATGGCAATTCTATATGG
GAGGTGTTTTCGATTTCCCATGTGGTCAAACTTTAGATCACGGTATCTTAATTGTTGGTT
ATGGTGCTCAAGATACCATCGTCGGTAAAAATACTCCATACTGGATCATTAAAAACTCAT
GGGGTGCCGATTGGGGTGAAGCTGGTTACTTAAAAGTTGAAAGAAATACTGATAAATGTG
GTGTTGCCAATTTCGTTTNCTTCATCAATTGTTGGTTCATCAAACTAAAAACAATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
cwptgit*fl*KRKKMRFILFFVLMLTALAAGRRLSVEESQFIAFQNKYNKIYSAEEYLV
KFETFKSNLLNIDALNKQATTIGSDTKFGVNKFADLSKEEFKKYYLSSKEARLTDDLP--
-

---YPYTAVDGECKFNSAQVGAKISSFTMVPQNETQIASYLFNNGPLAIAADAEEWQFYM
GGVFDFPCGQTLDHGILIVGYGAQDTIVGKNTPYWIIKNSWGADWGEAGYLKVERNTDKC
GVANFVXFINCWFIKLKTI
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxlhsfykkgkk*dssyslf*c*qpwllvedyqlrkvnslpskiniikfiqlkni*
lnlkpsnqiy*ilmp*tnkpppldlilnlvstnllisqkknsknit*aakkpv*lmts--
-

---pihtlllmenvnltlpklvlkfhlslwfhkmklkllptystmvh*llqlmlkngnsi
wevfsishvvkl*itvs*llvmvlkipssvkilhtgslkthgvpigvklvt*klkeilin
vvlpisfxssivgssn*kq*

Frame B:
llaywxyivfikkekneihlilcfnvnslgcw*kiis*gksihclpk*i**nlfs*rifs
*i*nlqikfiky*clkqtshhhwi*y*iwcqqic*slkrriqkillkqqrspfn**pp--
-

---lsihcc*wrm*i*lcpswc*nfifhygstk*nsncflliqqwsisycs*c*rmaily
grcfrfpmwsnfrsrylncwlwcsryhrr*kysildh*klmgcrlg*swllks*kky**m
wccqfrxlhqllvhqtknnk

Frame C:
cwptgit*fl*KRKKMRFILFFVLMLTALAAGRRLSVEESQFIAFQNKYNKIYSAEEYLV
KFETFKSNLLNIDALNKQATTIGSDTKFGVNKFADLSKEEFKKYYLSSKEARLTDDLP--
-

---YPYTAVDGECKFNSAQVGAKISSFTMVPQNETQIASYLFNNGPLAIAADAEEWQFYM
GGVFDFPCGQTLDHGILIVGYGAQDTIVGKNTPYWIIKNSWGADWGEAGYLKVERNTDKC
GVANFVXFINCWFIKLKTI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC481 (VFC481Q) /CSM/VF/VFC4-D/VFC481Q.Seq.d/                       1382   0.0
VSB747 (VSB747Q) /CSM/VS/VSB7-B/VSB747Q.Seq.d/                        801   0.0
VFO273 (VFO273Q) /CSM/VF/VFO2-D/VFO273Q.Seq.d/                        801   0.0
VFN261 (VFN261Q) /CSM/VF/VFN2-C/VFN261Q.Seq.d/                        801   0.0
VFN103 (VFN103Q) /CSM/VF/VFN1-A/VFN103Q.Seq.d/                        801   0.0
VFM871 (VFM871Q) /CSM/VF/VFM8-C/VFM871Q.Seq.d/                        801   0.0
VFM847 (VFM847Q) /CSM/VF/VFM8-B/VFM847Q.Seq.d/                        801   0.0
VFM117 (VFM117Q) /CSM/VF/VFM1-A/VFM117Q.Seq.d/                        801   0.0
VFL786 (VFL786Q) /CSM/VF/VFL7-D/VFL786Q.Seq.d/                        801   0.0
VFL771 (VFL771Q) /CSM/VF/VFL7-C/VFL771Q.Seq.d/                        801   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.      50  1e-12  4
BG524924|BG524924.1 1-26 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    60  4e-05  1
BG524136|BG524136.1 39-26 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    60  4e-05  1
BG525215|BG525215.1 45-7 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    60  4e-05  1
BG523152|BG523152.1 29-47 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    60  4e-05  1
BG524692|BG524692.1 6-13 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    60  4e-05  1
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.    46  1e-04  3
L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.    46  4e-04  3
BQ514757|BQ514757.1 EST622172 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMIN86 3' end, mRNA sequence.    42  0.005  3
BQ514756|BQ514756.1 EST622171 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMIN86 5' end, mRNA sequence.    42  0.005  3
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04988) RecName: Full=Cysteine proteinase 1;          EC=3.4.22...   184   3e-45
U42758_1(U42758|pid:none) Naegleria fowleri cysteine proteinase ...   130   5e-29
AB270920_1(AB270920|pid:none) Phaseolus vulgaris CP2 gene for cy...   130   5e-29
T12040(T12040) cysteine proteinase (EC 3.4.22.-) 2 precursor - k...   127   3e-28
BT041678_1(BT041678|pid:none) Zea mays full-length cDNA clone ZM...   126   7e-28
FB844670_1(FB844670|pid:none) Sequence 63943 from Patent WO20080...   126   7e-28
FB844658_1(FB844658|pid:none) Sequence 63931 from Patent WO20080...   126   7e-28
FJ609256_1(FJ609256|pid:none) Solanum lycopersicum cysteine prot...   126   9e-28
AF411121_1(AF411121|pid:none) Sandersonia aurantiaca cysteine pr...   125   2e-27
AY841792_1(AY841792|pid:none) Triticum aestivum cysteine proteas...   124   3e-27
protein update 2009. 6.17
PSORT 

 psg: 1.02  gvh: 0.83  alm: 0.38  top: 0.13  tms: 0.00  mit: 0.47  mip: 0.05
 nuc: 0.04  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for VFC481 is exc
5' end seq. ID VFC481F
5' end seq. 
>VFC481F.Seq
ACTGTTGGCCTACTGGNATTACATAGTTTTTATAAAAAAGGAAAAAAATGAGATTCATCT
TATTCTTTGTTTTAATGTTAACAGCCTTGGCTGCTGGTAGAAGATTATCAGTTGAGGAAA
GTCAATTCATTGCCTTCCAAAATAAATATAATAAAATTTATTCAGCTGAAGAATATTTAG
TTAAATTTGAAACCTTCAAATCAAATTTATTAAATATTGATGCCTTAAACAAACAAGCCA
CCACCATTGGATCTGATACTAAATTTGGTGTCAACAAATTTGCTGATCTCTCAAAAGAAG
AATTCAAAAAATATTACTTAAGCAGCAAAGAAGCCCGTTTAACTGATGACCTCCCA----
------
Length of 5' end seq. 356
3' end seq. ID VFC481Z
3' end seq. 
>VFC481Z.Seq
----------CCTATCCATACACTGCTGTTGATGGAGAATGTAAATTTAACTCTGCCCAA
GTTGGTGCTAAAATTTCATCTTTCACTATGGTTCCACAAAATGAAACTCAAATTGCTTCC
TACTTATTCAACAATGGTCCATTAGCTATTGCAGCTGATGCTGAAGAATGGCAATTCTAT
ATGGGAGGTGTTTTCGATTTCCCATGTGGTCAAACTTTAGATCACGGTATCTTAATTGTT
GGTTATGGTGCTCAAGATACCATCGTCGGTAAAAATACTCCATACTGGATCATTAAAAAC
TCATGGGGTGCCGATTGGGGTGAAGCTGGTTACTTAAAAGTTGAAAGAAATACTGATAAA
TGTGGTGTTGCCAATTTCGTTTNCTTCATCAATTGTTGGTTCATCAAACTAAAAACAATA
AA
Length of 3' end seq. 412
Connected seq. ID VFC481P
Connected seq. 
>VFC481P.Seq
ACTGTTGGCCTACTGGNATTACATAGTTTTTATAAAAAAGGAAAAAAATGAGATTCATCT
TATTCTTTGTTTTAATGTTAACAGCCTTGGCTGCTGGTAGAAGATTATCAGTTGAGGAAA
GTCAATTCATTGCCTTCCAAAATAAATATAATAAAATTTATTCAGCTGAAGAATATTTAG
TTAAATTTGAAACCTTCAAATCAAATTTATTAAATATTGATGCCTTAAACAAACAAGCCA
CCACCATTGGATCTGATACTAAATTTGGTGTCAACAAATTTGCTGATCTCTCAAAAGAAG
AATTCAAAAAATATTACTTAAGCAGCAAAGAAGCCCGTTTAACTGATGACCTCCCA----
------CCTATCCATACACTGCTGTTGATGGAGAATGTAAATTTAACTCTGCCCAAGTTG
GTGCTAAAATTTCATCTTTCACTATGGTTCCACAAAATGAAACTCAAATTGCTTCCTACT
TATTCAACAATGGTCCATTAGCTATTGCAGCTGATGCTGAAGAATGGCAATTCTATATGG
GAGGTGTTTTCGATTTCCCATGTGGTCAAACTTTAGATCACGGTATCTTAATTGTTGGTT
ATGGTGCTCAAGATACCATCGTCGGTAAAAATACTCCATACTGGATCATTAAAAACTCAT
GGGGTGCCGATTGGGGTGAAGCTGGTTACTTAAAAGTTGAAAGAAATACTGATAAATGTG
GTGTTGCCAATTTCGTTTNCTTCATCAATTGTTGGTTCATCAAACTAAAAACAATAAA
Length of connected seq. 768
Full length Seq ID -
Full length Seq. -
Length of full length seq. -