NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC480
Library VF
(Link to library)
Clone ID VFC480
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16182-1
Original site URL
Representative seq. ID VFC480P
(Link to Original site)
Representative DNA sequence 
>VFC480 (VFC480Q) /CSM/VF/VFC4-D/VFC480Q.Seq.d/
TTGGCCTACTGGNAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAA
ATGAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCA
CAAATACCAGNATAATGATCCAGAATTATGTCAACAAAGNAATTCAAAGAGAGGATTGTC
CACAAACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATA
TGCAAGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATC
ATTCAGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATG
CTTTCTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATG
AACTTTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATT
GGGATAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACAATTAATGGTACTC
AATTAAATCAATATATCGAATGGATGCAACAATATAATATTTCXXXXXXXXXXGTANACC
AAACATGGGACGTNTGGAACAATTATGGNCAANATTTGTTTGTTCCATCAACAACATNTG
ATGATTTCNCTCAACAATCAATNGAACATTTAGGTAGTATTGGTATTAATTANAATTGTT
CAACAGTTTTCAAAANAGATTACNTTAATCTTTTCNCTGAAAAACCCCAACCNATCGATT
TCNCANAACATTATGATGANATCNTTAAATTCTANGAANCTGNATTANAAATTAAANAAG
GCTCAATTGCNCAAATCNTTGAAANAATTTTATCAATCNTTGGTTTAAANAAANANATTT
ACGNTCAAGGTGAATATTATTTATTANAATTAAATTATCCNTTTATGAATGCNAAATATG
ATTATATCNCNTTACCAGGNTGNCCAGCAAATGGGATCGATTATGAAAANGGCNATTTTA
TTCATCNCNATTAGATTATTTTAAAATTAGATTATTTTAAATTAAATTGGNTATNAATAA
AAACCAAAAACCAAAATAATAAAAAAAAAATNNCTTTTTTTTTTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
gllxkinsctykn*NKRKKNEKDDNNIIICIIICNICFITNTXIMIQNYVNKXIQREDCP
QTPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDA
FFEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTYMGTINGTQ
LNQYIEWMQQYNI---

---VXQTWDVWNNYGQXLFVPSTTXDDFXQQSXEHLGSIGINXNCSTVFKXDYXNLFXEK
PQPIDFXXHYDXIXKFXEXXLXIKXGSIAQIXEXILSIXGLXKXIYXQGEYYLLXLNYPF
MNAKYDYIXLPGXPANGIDYEXGXFIHXX*iilkldyfklnwxxiktknqnnkkkxxfff
Translated Amino Acid sequence (All Frames) 
Frame A:
laywxk*thvhikikikekkmkrtiiillfvslfvtfvlsqipx**srimstkxfkeriv
hkhqyhgehsmtmtkskstickhqfskqfleislvswvdiiqqlvsmtlqlv*iiqqnmm
lslk*emvhyqis*msmvkmnfygvmlvsfvlfhtlmkligiktsiqllqkliweqlmvl
n*inisngcnniif---

---VXQTWDVWNNYGQXLFVPSTTXDDFXQQSXEHLGSIGINXNCSTVFKXDYXNLFXEK
PQPIDFXXHYDXIXKFXEXXLXIKXGSIAQIXEXILSIXGLXKXIYXQGEYYLLXLNYPF
MNAKYDYIXLPGXPANGIDYEXGXFIHXX*iilkldyfklnwxxiktknqnnkkkxxfff

Frame B:
wptgknklmyi*klk*kkkk*kgr**yyylyhyl*hlfyhkyqxndpelcqqxnskrgls
tntstmgniq*q*rnrsllyastsfrssfwkflw*vgwisfsnwfl*pynwfklysri*c
fl*srkwyitkyhkcrw*r*tfmv*cwyplycsih**nllg*khlfncfknlygnn*wys
iksiyrmdati*yf---

---xtkhgtxgtimxxiclfhqqhxmislnnqxni*vvlvlixivqqfskxitlifslkn
pnxsisxnimmxslnsxxlxxklxkaqlxkslkxfyqslv*xxxftxkvniiyxn*iixl
*mxnmiisxyqxxqqmgsimkxailfixirlf*n*iiln*igyx*kpktkiikkkxlffl

Frame C:
gllxkinsctykn*NKRKKNEKDDNNIIICIIICNICFITNTXIMIQNYVNKXIQREDCP
QTPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDA
FFEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTYMGTINGTQ
LNQYIEWMQQYNI---

---xpnmgrxeqlwxxfvcsinnx**fxstinxtfr*ywy*lxlfnsfqxrlx*sfx*kt
ptxrfxxtl**xx*ilxxxixn*xrlncxnx*xnfinxwfkxxxlrsr*ilfixiklsxy
ecxi*lyxxtrxxskwdrl*kxxfyssxldyfkirlf*iklxxnknqkpk**kknxfff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC480 (VFC480Q) /CSM/VF/VFC4-D/VFC480Q.Seq.d/                       1604   0.0
VHL857 (VHL857Q) /CSM/VH/VHL8-C/VHL857Q.Seq.d/                       1392   0.0
SSG340 (SSG340Q) /CSM/SS/SSG3-B/SSG340Q.Seq.d/                       1100   0.0
SLE504 (SLE504Q) /CSM/SL/SLE5-A/SLE504Q.Seq.d/                       1082   0.0
VFL220 (VFL220Q) /CSM/VF/VFL2-A/VFL220Q.Seq.d/                        991   0.0
VFJ281 (VFJ281Q) /CSM/VF/VFJ2-D/VFJ281Q.Seq.d/                        991   0.0
VFG895 (VFG895Q) /CSM/VF/VFG8-D/VFG895Q.Seq.d/                        985   0.0
FC-AD10 (FC-AD10Q) /CSM/FC/FC-AD/FC-AD10Q.Seq.d/                      912   0.0
FC-080 (FC-080Q) /CSM/CG/CG-FC/FC-080Q.Seq.d/                         912   0.0
SSA660 (SSA660Q) /CSM/SS/SSA6-C/SSA660Q.Seq.d/                        892   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   839  0.0  7
U50311|U50311.1 Caenorhabditis elegans cosmid C25E10, complete sequence.    40  0.20  4
AZ179765|AZ179765.1 SP_0166_B1_A11_SP6E Strongylocentrotus purpuratus, purple sea urchin, sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=166 Col=21 Row=B, DNA sequence.    48  0.24  1
BH334420|BH334420.1 CH230-110K10.TJ CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-110K10, DNA sequence.    48  0.24  1
BH318036|BH318036.1 CH230-196J14.TJ CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-196J14, DNA sequence.    48  0.24  1
AC126501|AC126501.4 Rattus norvegicus clone CH230-206K3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48  0.24  1
AL034557|AL034557.8 Plasmodium falciparum MAL4P1.                 52  0.42  7
AE015934|AE015934.1 Staphylococcus epidermidis ATCC 12228 plasmid pSE-12228-05, complete sequence.    42  0.72  2
AC102777|AC102777.3 Mus musculus clone RP23-5K7, WORKING DRAFT SEQUENCE, 9 unordered pieces.    46  0.95  1
AC123538|AC123538.3 Sminthopsis macroura clone 79G10, complete sequence.    42  1.4  3
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553W9) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5 ...   532   e-150
AC116305_28(AC116305|pid:none) Dictyostelium discoideum chromoso...   306   5e-82
(Q1ZYR0) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    96   3e-18
AF068227_1(AF068227|pid:none) Homo sapiens putative transmembran...    95   4e-18
AK032293_1(AK032293|pid:none) Mus musculus adult male olfactory ...    94   1e-17
BC025487_1(BC025487|pid:none) Mus musculus ceroid-lipofuscinosis...    94   1e-17
AK075109_1(AK075109|pid:none) Homo sapiens cDNA FLJ90628 fis, cl...    93   1e-17
AY885123_1(AY885123|pid:none) Canis familiaris CLN5 (CLN5) gene,...    93   1e-17
(Q5JZQ9) Ceroid-lipofuscinosis neuronal protein 5 precursor (Pro...    93   1e-17
CR761663_1(CR761663|pid:none) Xenopus tropicalis finished cDNA, ...    91   6e-17
protein update 2009. 6.17
PSORT 

 psg: 0.99  gvh: 0.33  alm: 0.26  top: 0.33  tms: 0.07  mit: 0.23  mip: 0.02
 nuc: 0.04  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: mitochondrial
	 16.0 %: Golgi
	 16.0 %: nuclear
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VFC480 is mit
5' end seq. ID VFC480F
5' end seq. 
>VFC480F.Seq
TTGGCCTACTGGNAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAA
ATGAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCA
CAAATACCAGNATAATGATCCAGAATTATGTCAACAAAGNAATTCAAAGAGAGGATTGTC
CACAAACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATA
TGCAAGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATC
ATTCAGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATG
CTTTCTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATG
AACTTTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATT
GGGATAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACAATTAATGGTACTC
AATTAAATCAATATATCGAATGGATGCAACAATATAATATTTC----------
Length of 5' end seq. 583
3' end seq. ID VFC480Z
3' end seq. 
>VFC480Z.Seq
----------GTANACCAAACATGGGACGTNTGGAACAATTATGGNCAANATTTGTTTGT
TCCATCAACAACATNTGATGATTTCNCTCAACAATCAATNGAACATTTAGGTAGTATTGG
TATTAATTANAATTGTTCAACAGTTTTCAAAANAGATTACNTTAATCTTTTCNCTGAAAA
ACCCCAACCNATCGATTTCNCANAACATTATGATGANATCNTTAAATTCTANGAANCTGN
ATTANAAATTAAANAAGGCTCAATTGCNCAAATCNTTGAAANAATTTTATCAATCNTTGG
TTTAAANAAANANATTTACGNTCAAGGTGAATATTATTTATTANAATTAAATTATCCNTT
TATGAATGCNAAATATGATTATATCNCNTTACCAGGNTGNCCAGCAAATGGGATCGATTA
TGAAAANGGCNATTTTATTCATCNCNATTAGATTATTTTAAAATTAGATTATTTTAAATT
AAATTGGNTATNAATAAAAACCAAAAACCAAAATAATAAAAAAAAAATNNCTTTTTTTTT
TA
Length of 3' end seq. 532
Connected seq. ID VFC480P
Connected seq. 
>VFC480P.Seq
TTGGCCTACTGGNAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAA
ATGAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCA
CAAATACCAGNATAATGATCCAGAATTATGTCAACAAAGNAATTCAAAGAGAGGATTGTC
CACAAACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATA
TGCAAGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATC
ATTCAGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATG
CTTTCTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATG
AACTTTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATT
GGGATAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACAATTAATGGTACTC
AATTAAATCAATATATCGAATGGATGCAACAATATAATATTTC----------GTANACC
AAACATGGGACGTNTGGAACAATTATGGNCAANATTTGTTTGTTCCATCAACAACATNTG
ATGATTTCNCTCAACAATCAATNGAACATTTAGGTAGTATTGGTATTAATTANAATTGTT
CAACAGTTTTCAAAANAGATTACNTTAATCTTTTCNCTGAAAAACCCCAACCNATCGATT
TCNCANAACATTATGATGANATCNTTAAATTCTANGAANCTGNATTANAAATTAAANAAG
GCTCAATTGCNCAAATCNTTGAAANAATTTTATCAATCNTTGGTTTAAANAAANANATTT
ACGNTCAAGGTGAATATTATTTATTANAATTAAATTATCCNTTTATGAATGCNAAATATG
ATTATATCNCNTTACCAGGNTGNCCAGCAAATGGGATCGATTATGAAAANGGCNATTTTA
TTCATCNCNATTAGATTATTTTAAAATTAGATTATTTTAAATTAAATTGGNTATNAATAA
AAACCAAAAACCAAAATAATAAAAAAAAAATNNCTTTTTTTTTTA
Length of connected seq. 1115
Full length Seq ID -
Full length Seq. -
Length of full length seq. -