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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC463
Library VF
(Link to library)
Clone ID VFC463
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFC463P
(Link to Original site)
Representative DNA sequence 
>VFC463 (VFC463Q) /CSM/VF/VFC4-C/VFC463Q.Seq.d/
ACTGTTGGCCTACTGGNACTTAATTCAAATATATATATATAAATCATTTAAAAATGGGAC
GGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTT
GCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCAXXXXXXXXXXCGTTGCCCAGAAGCCCTCTTCCAACCATCA
TTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGT
GATGTTGATATCCCGTAAAGATTTATGCGGTAACGTCGTCTTATCCGGTGGTACAACTAT
GTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCAT
GAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTAT
CTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCNCCAAAGAAGAATACGACGAATC
AGGTCCATCAATTGTTCACAGAAAATGTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllxlnsniyi*ii*KWDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHT
GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWH---

---RCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIP*rfmr*rrlirwynyvpryc*s
yeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdxqrrirrirsincsq
km
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxlnsniyi*ii*KWDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHT
GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWH---

---RCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIP*rfmr*rrlirwynyvpryc*s
yeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdxqrrirrirsincsq
km

Frame B:
llaywxliqiyiyksfkngtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtl
vlwsvwvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgi---

---vaqkpssnhhs*vwnllvstkppttps*nvmlisrkdlcgnvvlsggttmfpgiadr
mnkeltalapstmkikiiapperkysvwiggsilaslstfqqmwixkeeydesgpsivhr
kc

Frame C:
cwptgt*fkyiyinhlkmgr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthw
cygrygskrlicr**spikerylnpqipn*tryryqlg*ygknlas---

---lprsplptiilrygicwyprnhlqlhhem*c*ypvkiyavtssypvvqlcsqvlliv
*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgspkknttnqvhqlfte
nv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC463 (VFC463Q) /CSM/VF/VFC4-C/VFC463Q.Seq.d/                       1219   0.0
VHH249 (VHH249Q) /CSM/VH/VHH2-C/VHH249Q.Seq.d/                        682   0.0
VFO395 (VFO395Q) /CSM/VF/VFO3-D/VFO395Q.Seq.d/                        682   0.0
VFO115 (VFO115Q) /CSM/VF/VFO1-A/VFO115Q.Seq.d/                        682   0.0
VFN676 (VFN676Q) /CSM/VF/VFN6-D/VFN676Q.Seq.d/                        682   0.0
VFN217 (VFN217Q) /CSM/VF/VFN2-A/VFN217Q.Seq.d/                        682   0.0
VFM814 (VFM814Q) /CSM/VF/VFM8-A/VFM814Q.Seq.d/                        682   0.0
VFM723 (VFM723Q) /CSM/VF/VFM7-A/VFM723Q.Seq.d/                        682   0.0
VFM650 (VFM650Q) /CSM/VF/VFM6-C/VFM650Q.Seq.d/                        682   0.0
VFM579 (VFM579Q) /CSM/VF/VFM5-D/VFM579Q.Seq.d/                        682   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      523  0.0  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   500  0.0  4
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        484  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   484  0.0  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   468  0.0  4
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   436  0.0  8
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   404  0.0  4
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     381  0.0  3
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   412  0.0  4
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   315  0.0  3
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A61043(A61043)actin CA15 - sea squirt (Styela clava) (fragments)      191   9e-87
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   185   7e-57
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   181   7e-57
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        181   7e-57
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   185   7e-57
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   184   3e-56
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   185   6e-56
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   164   2e-55
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   169   3e-55
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          176   4e-55
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFC463 is nuc
5' end seq. ID VFC463F
5' end seq. 
>VFC463F.Seq
ACTGTTGGCCTACTGGNACTTAATTCAAATATATATATATAAATCATTTAAAAATGGGAC
GGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTT
GCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCA----------
Length of 5' end seq. 320
3' end seq. ID VFC463Z
3' end seq. 
>VFC463Z.Seq
----------CGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGC
TGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCCGTAAAG
ATTTATGCGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATC
GTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTC
CACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTT
TCCAACAAATGTGGATCNCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACA
GAAAATGTT
Length of 3' end seq. 359
Connected seq. ID VFC463P
Connected seq. 
>VFC463P.Seq
ACTGTTGGCCTACTGGNACTTAATTCAAATATATATATATAAATCATTTAAAAATGGGAC
GGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTT
GCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCA----------CGTTGCCCAGAAGCCCTCTTCCAACCATCA
TTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGT
GATGTTGATATCCCGTAAAGATTTATGCGGTAACGTCGTCTTATCCGGTGGTACAACTAT
GTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCAT
GAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTAT
CTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCNCCAAAGAAGAATACGACGAATC
AGGTCCATCAATTGTTCACAGAAAATGTT
Length of connected seq. 679
Full length Seq ID -
Full length Seq. -
Length of full length seq. -