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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC392
Library VF
(Link to library)
Clone ID VFC392
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1
Original site URL
Representative seq. ID VFC392P
(Link to Original site)
Representative DNA sequence 
>VFC392 (VFC392Q) /CSM/VF/VFC3-D/VFC392Q.Seq.d/
ACTGTTGGCCTACTGGTAATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGC
CAATTAGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCAT
TAAGACAAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAA
TTGCAAAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTG
CATGTAAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATT
TGGATAGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATG
GTAAAGATGTTTATGAAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTG
AAGGTGTTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGAXXXXXXXX
XXTCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTCA
CCAAANATATGATTGACTCACTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTG
TACTCGGTCCANAAGGTGGTGTAGATCCATACAAACTCCCAANAGAATTAGGTATTTACA
AAGAAGTTAAACATACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAAGAATCATTGATA
ATAGACTTCAATATGAAGCTCGTAATCGTAAAAAAGAAGCAAAAGAAATTAATTTCATTG
AACAATCAAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACAAAAAAAAAAAAA
AANAAAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
cwptgn*QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEI
AKNKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADG
KDVYEGIKKSGKFRFIKRTEGVSTTELVGRMLLCTK---

---SVLSCRYVDEVVIGAPFSVTKXMIDSLHINVVVHGDDQVVLGPXGGVDPYKLPXELG
IYKEVKHTEGXXATEIVKRIIDNRLQYEARNRKKEAKEINFIEQSN*kklknkk*k*nkk
kkkxk
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvinkflq*vlqliksqlefmlmvvli*cildmqmh*dkqen*eif**lvyiqmkk
lqkikvhql*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifqlvqm
vkmfmkalkrvvnldssnvlkvfqqpswlvvcyyapk---

---SVLSCRYVDEVVIGAPFSVTKXMIDSLHINVVVHGDDQVVLGPXGGVDPYKLPXELG
IYKEVKHTEGXXATEIVKRIIDNRLQYEARNRKKEAKEINFIEQSN*kklknkk*k*nkk
kkkxk

Frame B:
llayw*ltsfyneyyn**kan*slc*wlf*fnafwtckciktskrirryfsswctyr*rn
ckk*rstsye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfswcrw
*rcl*rh*kew*i*ihqty*rcfnnrvgwsyvimhqr---

---lyshvvmsmklslvlhsvspxi*lthftsmlsfmvmiklysvxkvv*ihtnsqxn*v
ftkklnilkvxlqlkslkesliidfnmklvivkkkqkklislnnqikkn*kiknknktkk
kkxkk

Frame C:
cwptgn*QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEI
AKNKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADG
KDVYEGIKKSGKFRFIKRTEGVSTTELVGRMLLCTK---

---ctlmslcr*schwcsiqchqxyd*ltshqccrsw**ssctrsxrwcrsiqtpxriry
lqrs*ty*rsxcn*nr*knh***tsi*ss*s*krskrn*fh*tiklkkikk*kikikqkk
kkxkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC392 (VFC392Q) /CSM/VF/VFC3-D/VFC392Q.Seq.d/                       1471   0.0
VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                        912   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                        904   0.0
VFF663 (VFF663Q) /CSM/VF/VFF6-C/VFF663Q.Seq.d/                        902   0.0
VFE318 (VFE318Q) /CSM/VF/VFE3-A/VFE318Q.Seq.d/                        902   0.0
VFO305 (VFO305Q) /CSM/VF/VFO3-A/VFO305Q.Seq.d/                        900   0.0
VFM413 (VFM413Q) /CSM/VF/VFM4-A/VFM413Q.Seq.d/                        896   0.0
VFL367 (VFL367Q) /CSM/VF/VFL3-C/VFL367Q.Seq.d/                        896   0.0
VFI626 (VFI626Q) /CSM/VF/VFI6-B/VFI626Q.Seq.d/                        896   0.0
VFG581 (VFG581Q) /CSM/VF/VFG5-D/VFG581Q.Seq.d/                        894   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP17G1 5' sequence, mRNA sequence.    92  1e-16  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    92  2e-16  3
AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    92  2e-15  2
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    92  2e-15  2
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    92  2e-15  2
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    92  2e-15  2
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    92  2e-15  2
BG642411|BG642411.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end, mRNA sequence.    92  2e-15  2
BG601025|BG601025.1 EST505920 cSTS Solanum tuberosum cDNA clone cSTS30D8 5' sequence, mRNA sequence.    90  9e-15  2
BI935302|BI935302.1 EST555191 tomato flower, anthesis Lycopersicon esculentum cDNA clone cTOD22O12 5' end, mRNA sequence.    92  1e-14  1
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   301   2e-80
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   197   5e-49
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   196   8e-49
AL663030_11(AL663030|pid:none) Mouse DNA sequence from clone RP2...   196   8e-49
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   196   8e-49
BC088018_1(BC088018|pid:none) Xenopus tropicalis phosphate cytid...   192   9e-48
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   192   1e-47
BT077764_1(BT077764|pid:none) Lepeophtheirus salmonis clone lsal...   190   4e-47
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   186   6e-46
AC087079_11(AC087079|pid:none) Caenorhabditis elegans cosmid Y37...   185   2e-45
protein update 2009. 6.17
PSORT 

 psg: 0.52  gvh: 0.53  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.04
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFC392 is nuc
5' end seq. ID VFC392F
5' end seq. 
>VFC392F.Seq
ACTGTTGGCCTACTGGTAATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGC
CAATTAGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCAT
TAAGACAAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAA
TTGCAAAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTG
CATGTAAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATT
TGGATAGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATG
GTAAAGATGTTTATGAAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTG
AAGGTGTTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGA--------
--
Length of 5' end seq. 472
3' end seq. ID VFC392Z
3' end seq. 
>VFC392Z.Seq
----------TCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATT
CAGTGTCACCAAANATATGATTGACTCACTTCACATCAATGTTGTCGTTCATGGTGATGA
TCAAGTTGTACTCGGTCCANAAGGTGGTGTAGATCCATACAAACTCCCAANAGAATTAGG
TATTTACAAAGAAGTTAAACATACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAAGAAT
CATTGATAATAGACTTCAATATGAAGCTCGTAATCGTAAAAAAGAAGCAAAAGAAATTAA
TTTCATTGAACAATCAAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACAAAAA
AAAAAAAAAANAAAAAAA
Length of 3' end seq. 368
Connected seq. ID VFC392P
Connected seq. 
>VFC392P.Seq
ACTGTTGGCCTACTGGTAATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGC
CAATTAGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCAT
TAAGACAAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAA
TTGCAAAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTG
CATGTAAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATT
TGGATAGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATG
GTAAAGATGTTTATGAAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTG
AAGGTGTTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGA--------
--TCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTCA
CCAAANATATGATTGACTCACTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTG
TACTCGGTCCANAAGGTGGTGTAGATCCATACAAACTCCCAANAGAATTAGGTATTTACA
AAGAAGTTAAACATACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAAGAATCATTGATA
ATAGACTTCAATATGAAGCTCGTAATCGTAAAAAAGAAGCAAAAGAAATTAATTTCATTG
AACAATCAAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACAAAAAAAAAAAAA
AANAAAAAAA
Length of connected seq. 840
Full length Seq ID -
Full length Seq. -
Length of full length seq. -