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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM227
Library AF
(Link to library)
Clone ID AFM227
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM227P
(Link to Original site)
Representative DNA sequence 
>AFM227 (AFM227Q) /CSM/AF/AFM2-B/AFM227Q.Seq.d/
AATAATCATATTCATAGAAATTTATTATTAGAATCATTAAATTCATTTGAAACTTTAACA
AAATCTGAATTATTTGATAAATCATTAGCAATTTATTTAATTTTTAATACAAGTGATATC
TTTTATGAAAATATTAAACAATATGATTTAAAATCTTGTTTCTCTGAATATGAAGGTGGT
AATGATCCTGAAAAAGCTGTATCTTTTATATCAAATAAATTTACAAAATTCTTACAAAAT
AAAGATAAACCATATAAAAGTCATATTGTAAATTTATTAGATAAAAATAATGTTAGAGAA
GAGTTTGAAGGTATTTTTGATAGTTTAAAAATTGATGCAGAGAAAAGAGGTTTTACAACA
CCTTATAATCAATCAAATTCATCACCAGTTTCATCAATTGGTTCAAATTCATCTAGGAAT
AGTCGTTTACCAAATACTTCAGTTTCAATACCTGGATTATATAGTAGTGATAATGATAAT
ACTCGACTAAAAAATGTAAATAATAATAATAATAACAACAATAATACAACAACATATGGA
TCATCAACATTCCCAAGXXXXXXXXXXATGGGGGCAGTANCCTACAATGGTCCACCAAAG
GATATACCAATCTCATACTATGCAGTTTATGATGGTCATGGTGGTACTGAAACCTCAACA
CTCTTGGAGCCAACAGTTCATAATTGTTTGGTCAATTCTCAAAGTTTCCGAGATGGTGAC
TATGAACAAGCTTTCCGTGATGCATACGCTGAAGCTGATGATATTGTAATTGAAAAATGT
GAAAAGAGTGGTAGCACAGGTGTATCGGCTCTATTGGTTGGAAATAAACTCTACACTGCA
AATGTAGGCGATTCAGAGATAGTTTTAGCACGTGCTCAACCAAATGCAAATCCTAAAGGA
CCCGTCACCTATGAACCGGTTTTACTCTCCTACAAACATTTGGCAAGTGATGACCAAGAA
AAGAAGAGAGTCACCGATTTGGGTGGTATGATCATTTTCAATCGTTTGTTTGGTTCATTG
GCTGTTAGTAGATCGTTTGGTGATAAGGAGTACAAAGAGGGTGAAAAGAAGTTTTGTGTC
TCATGATCCTTATCAAACAACAACCGATCTCACTGCTCGTGATCATTTCTTCATTTTGGC
TTGTGATGGTCTTTGGGATAAGGTCGAATATGATGAAGCCGTTCAATTTGTTCAAAGAAA
TATAAAATTGGGAAAAAGTGCAACTGAAATTTCAGAATTATTAGCTCAAGATTCTTANAT
AGAGGTTCAGGNATAATATCAC
sequence update 2009. 4. 3
Translated Amino Acid sequence 
NNHIHRNLLLESLNSFETLTKSELFDKSLAIYLIFNTSDIFYENIKQYDLKSCFSEYEGG
NDPEKAVSFISNKFTKFLQNKDKPYKSHIVNLLDKNNVREEFEGIFDSLKIDAEKRGFTT
PYNQSNSSPVSSIGSNSSRNSRLPNTSVSIPGLYSSDNDNTRLKNVNNNNNNNNNTTTYG
SSTFP---

---MGAVXYNGPPKDIPISYYAVYDGHGGTETSTLLEPTVHNCLVNSQSFRDGDYEQAFR
DAYAEADDIVIEKCEKSGSTGVSALLVGNKLYTANVGDSEIVLARAQPNANPKGPVTYEP
VLLSYKHLASDDQEKKRVTDLGGMIIFNRLFGSLAVSRSFGDKEYKEGEKKFCVS*slsn
nnrshcs*sflhfgl*wslg*gri**srsicskkykigkkcn*nfriissrflxrgsgii
s
Translated Amino Acid sequence (All Frames) 
Frame A:
NNHIHRNLLLESLNSFETLTKSELFDKSLAIYLIFNTSDIFYENIKQYDLKSCFSEYEGG
NDPEKAVSFISNKFTKFLQNKDKPYKSHIVNLLDKNNVREEFEGIFDSLKIDAEKRGFTT
PYNQSNSSPVSSIGSNSSRNSRLPNTSVSIPGLYSSDNDNTRLKNVNNNNNNNNNTTTYG
SSTFP---

---MGAVXYNGPPKDIPISYYAVYDGHGGTETSTLLEPTVHNCLVNSQSFRDGDYEQAFR
DAYAEADDIVIEKCEKSGSTGVSALLVGNKLYTANVGDSEIVLARAQPNANPKGPVTYEP
VLLSYKHLASDDQEKKRVTDLGGMIIFNRLFGSLAVSRSFGDKEYKEGEKKFCVS*slsn
nnrshcs*sflhfgl*wslg*gri**srsicskkykigkkcn*nfriissrflxrgsgii
s

Frame B:
iiifieiyy*nh*ihlkl*qnlnylinh*qfi*fliqvisfmkilnnmi*nlvslnmkvv
milkklyllyqinlqnsykikinhikvil*iy*ikimlekslkvfliv*klmqrkevlqh
liinqihhqfhqlvqihlgivvyqilqfqyldyivvimiild*km*iiiiittiiqqhmd
hqhsq---

---wgqxptmvhqriyqshtmqfmmvmvvlkpqhswsqqfiivwsilkvsemvtmnklsv
mhtlklmil*lknvkrvvaqvyrlywleinstlqm*aiqr*f*hvlnqmqilkdpspmnr
fysptniwqvmtkkrrespiwvv*sfsivclvhwllvdrlvirstkrvkrsfvshdpyqt
ttdltardhffilacdglwdkveydeavqfvqrniklgksateisellaqdsxievqx*y
h

Frame C:
*sys*kfiiriikfi*nfnki*ii**iisnlfnf*yk*yll*ky*ti*fkilfl*i*rw*
*s*kscifyik*iykiltk*r*ti*ksyckfir*k*c*rrv*ryf**fkn*crekrfynt
l*sikfitsfinwfkfi*e*sftkyfsfntwii******ystkkck*****qq*ynniwi
inipk---

---ggsxlqwstkgytnlilcsl*wswwy*nlntlganss*lfgqfskfprw*l*tsfp*
cir*s**ycn*km*kew*hrcigsigwk*tlhckcrrfrdsfstcstkcks*rtrhl*tg
ftllqtfgk**prkeeshrfgwydhfqsfvwfigc**ivw**gvqrg*kevlclmilikq
qpisllviissfwlvmvfgirsnmmkpfnlfkei*nwekvqlkfqny*lkilx*rfrxni
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM227 (AFM227Q) /CSM/AF/AFM2-B/AFM227Q.Seq.d/                       1421   0.0
SFG594 (SFG594Q) /CSM/SF/SFG5-D/SFG594Q.Seq.d/                       1384   0.0
CHR482 (CHR482Q) /CSM/CH/CHR4-D/CHR482Q.Seq.d/                       1342   0.0
CHE537 (CHE537Q) /CSM/CH/CHE5-B/CHE537Q.Seq.d/                       1314   0.0
AFE528 (AFE528Q) /CSM/AF/AFE5-B/AFE528Q.Seq.d/                       1296   0.0
AFA158 (AFA158Q) /CSM/AF/AFA1-C/AFA158Q.Seq.d/                       1245   0.0
AHK426 (AHK426Q) /CSM/AH/AHK4-B/AHK426Q.Seq.d/                       1191   0.0
CHN126 (CHN126Q) /CSM/CH/CHN1-B/CHN126Q.Seq.d/                       1185   0.0
SLE231 (SLE231Q) /CSM/SL/SLE2-B/SLE231Q.Seq.d/                        957   0.0
SHD445 (SHD445Q) /CSM/SH/SHD4-B/SHD445Q.Seq.d/                        753   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346854) Dictyostelium discoideum cDNA clone:dda25e08, 3' ...  1386   0.0    1
(AF019985) Dictyostelium discoideum Spalten (spnA) mRNA, com...  1001   0.0    2
(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...  1001   0.0    2
(BJ400804) Dictyostelium discoideum cDNA clone:dds14k23, 3' ...  1001   0.0    2
(BJ385982) Dictyostelium discoideum cDNA clone:ddc56d22, 3' ...   975   0.0    2
(BJ339772) Dictyostelium discoideum cDNA clone:dda10g07, 3' ...   944   0.0    2
(BJ342769) Dictyostelium discoideum cDNA clone:dda1c15, 3' e...   936   0.0    3
(BJ378266) Dictyostelium discoideum cDNA clone:ddc31i09, 3' ...   852   0.0    3
(BJ384098) Dictyostelium discoideum cDNA clone:ddc48c07, 3' ...   831   0.0    2
(BJ353739) Dictyostelium discoideum cDNA clone:dda51d08, 3' ...   831   0.0    2
dna update 2009. 4.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY328084_1(AY328084|pid:none) Hypocrea jecorina putative serine/...   102   4e-26
(Q09173) RecName: Full=Protein phosphatase 2C homolog 3;        ...    95   3e-25
CU640366_29(CU640366|pid:none) Podospora anserina genomic DNA ch...    99   7e-25
L34882_1(L34882|pid:none) Schizosaccharomyces pombe protein phos...    93   1e-24
BX294012_38(BX294012|pid:none) Neurospora crassa DNA linkage gro...    97   3e-24
AM920431_242(AM920431|pid:none) Penicillium chrysogenum Wisconsi...    94   3e-24
(Q09172) RecName: Full=Protein phosphatase 2C homolog 2;        ...    97   3e-24
CR382131_607(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...    90   2e-23
CP000079_240(CP000079|pid:none) Leishmania major strain Friedlin...    88   2e-22
AM502245_192(AM502245|pid:none) Leishmania infantum chromosome 27.     88   2e-22
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM227 is

5' end seq. ID AFM227F
5' end seq. 
>AFM227F.Seq
AATAATCATATTCATAGAAATTTATTATTAGAATCATTAAATTCATTTGAAACTTTAACA
AAATCTGAATTATTTGATAAATCATTAGCAATTTATTTAATTTTTAATACAAGTGATATC
TTTTATGAAAATATTAAACAATATGATTTAAAATCTTGTTTCTCTGAATATGAAGGTGGT
AATGATCCTGAAAAAGCTGTATCTTTTATATCAAATAAATTTACAAAATTCTTACAAAAT
AAAGATAAACCATATAAAAGTCATATTGTAAATTTATTAGATAAAAATAATGTTAGAGAA
GAGTTTGAAGGTATTTTTGATAGTTTAAAAATTGATGCAGAGAAAAGAGGTTTTACAACA
CCTTATAATCAATCAAATTCATCACCAGTTTCATCAATTGGTTCAAATTCATCTAGGAAT
AGTCGTTTACCAAATACTTCAGTTTCAATACCTGGATTATATAGTAGTGATAATGATAAT
ACTCGACTAAAAAATGTAAATAATAATAATAATAACAACAATAATACAACAACATATGGA
TCATCAACATTCCCAAGXXXXXXXXXX
Length of 5' end seq. 567
3' end seq. ID AFM227Z
3' end seq. 
>AFM227Z.Seq
XXXXXXXXXXATGGGGGCAGTANCCTACAATGGTCCACCAAAGGATATACCAATCTCATA
CTATGCAGTTTATGATGGTCATGGTGGTACTGAAACCTCAACACTCTTGGAGCCAACAGT
TCATAATTGTTTGGTCAATTCTCAAAGTTTCCGAGATGGTGACTATGAACAAGCTTTCCG
TGATGCATACGCTGAAGCTGATGATATTGTAATTGAAAAATGTGAAAAGAGTGGTAGCAC
AGGTGTATCGGCTCTATTGGTTGGAAATAAACTCTACACTGCAAATGTAGGCGATTCAGA
GATAGTTTTAGCACGTGCTCAACCAAATGCAAATCCTAAAGGACCCGTCACCTATGAACC
GGTTTTACTCTCCTACAAACATTTGGCAAGTGATGACCAAGAAAAGAAGAGAGTCACCGA
TTTGGGTGGTATGATCATTTTCAATCGTTTGTTTGGTTCATTGGCTGTTAGTAGATCGTT
TGGTGATAAGGAGTACAAAGAGGGTGAAAAGAAGTTTTGTGTCTCATGATCCTTATCAAA
CAACAACCGATCTCACTGCTCGTGATCATTTCTTCATTTTGGCTTGTGATGGTCTTTGGG
ATAAGGTCGAATATGATGAAGCCGTTCAATTTGTTCAAAGAAATATAAAATTGGGAAAAA
GTGCAACTGAAATTTCAGAATTATTAGCTCAAGATTCTTANATAGAGGTTCAGGNATAAT
ATCAC
Length of 3' end seq. 725
Connected seq. ID AFM227P
Connected seq. 
>AFM227P.Seq
AATAATCATATTCATAGAAATTTATTATTAGAATCATTAAATTCATTTGAAACTTTAACA
AAATCTGAATTATTTGATAAATCATTAGCAATTTATTTAATTTTTAATACAAGTGATATC
TTTTATGAAAATATTAAACAATATGATTTAAAATCTTGTTTCTCTGAATATGAAGGTGGT
AATGATCCTGAAAAAGCTGTATCTTTTATATCAAATAAATTTACAAAATTCTTACAAAAT
AAAGATAAACCATATAAAAGTCATATTGTAAATTTATTAGATAAAAATAATGTTAGAGAA
GAGTTTGAAGGTATTTTTGATAGTTTAAAAATTGATGCAGAGAAAAGAGGTTTTACAACA
CCTTATAATCAATCAAATTCATCACCAGTTTCATCAATTGGTTCAAATTCATCTAGGAAT
AGTCGTTTACCAAATACTTCAGTTTCAATACCTGGATTATATAGTAGTGATAATGATAAT
ACTCGACTAAAAAATGTAAATAATAATAATAATAACAACAATAATACAACAACATATGGA
TCATCAACATTCCCAAG----------ATGGGGGCAGTANCCTACAATGGTCCACCAAAG
GATATACCAATCTCATACTATGCAGTTTATGATGGTCATGGTGGTACTGAAACCTCAACA
CTCTTGGAGCCAACAGTTCATAATTGTTTGGTCAATTCTCAAAGTTTCCGAGATGGTGAC
TATGAACAAGCTTTCCGTGATGCATACGCTGAAGCTGATGATATTGTAATTGAAAAATGT
GAAAAGAGTGGTAGCACAGGTGTATCGGCTCTATTGGTTGGAAATAAACTCTACACTGCA
AATGTAGGCGATTCAGAGATAGTTTTAGCACGTGCTCAACCAAATGCAAATCCTAAAGGA
CCCGTCACCTATGAACCGGTTTTACTCTCCTACAAACATTTGGCAAGTGATGACCAAGAA
AAGAAGAGAGTCACCGATTTGGGTGGTATGATCATTTTCAATCGTTTGTTTGGTTCATTG
GCTGTTAGTAGATCGTTTGGTGATAAGGAGTACAAAGAGGGTGAAAAGAAGTTTTGTGTC
TCATGATCCTTATCAAACAACAACCGATCTCACTGCTCGTGATCATTTCTTCATTTTGGC
TTGTGATGGTCTTTGGGATAAGGTCGAATATGATGAAGCCGTTCAATTTGTTCAAAGAAA
TATAAAATTGGGAAAAAGTGCAACTGAAATTTCAGAATTATTAGCTCAAGATTCTTANAT
AGAGGTTCAGGNATAATATCAC
Length of connected seq. 1272
Full length Seq ID -
Full length Seq. -
Length of full length seq. -