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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC294
Library VF
(Link to library)
Clone ID VFC294
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1
Original site URL
Representative seq. ID VFC294P
(Link to Original site)
Representative DNA sequence 
>VFC294 (VFC294Q) /CSM/VF/VFC2-D/VFC294Q.Seq.d/
ACTGTTGGCCTACTGGATAAAAAATTAATTAACAAGTTTTTACAATGGAGTACTACAACT
AATAAAAAGCCAATTAGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACAT
GCAAATGCATTAAGACAAGCAAGGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATAC
AGATGAAGAAATTGCAAAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAA
AGCAGTACGTGCATGTAAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAAC
TGAAGAATATTTGGATAGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGT
TGGTGCAGATXXXXXXXXXXCTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTG
NACNCGGTCCANAAGGTGGTGTANATCCATACAAACNCCCAANAGAATTAGGTATTTACA
AANAAGTTAAACATACTGAAGGTCNCCCNGCAACTGAAATCGTTAAAAGAATCNTTGATA
ATAGACNTCAATATNAAGCNCGNAATCGTAAAAAANAAGCNAAAGAAATTAATTTCNTTG
AACGATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACCAATAAAAAAAAA
AATAGAAAAAAAATTTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywikn*ltsfyngvlqliksqlefmlmvvli*cildmqmh*DKQGELGDILVVGVHT
DEEIAKNKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISV
GAD---

---LHINVVVHGDDQVXXGPXGGVXPYKXPXELGIYKXVKHTEGXPATEIVKRIXDNRXQ
YXARNRKKXAKEINFXERSN*kklknkk*k*nq*kkk*kknl
Translated Amino Acid sequence (All Frames) 
Frame A:
tvglldkklinkflqwstttnkkpirvyvdgcfdlmhfghanalrqarrirryfsswcty
r*rnckk*rstsye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfs
wcr---

---LHINVVVHGDDQVXXGPXGGVXPYKXPXELGIYKXVKHTEGXPATEIVKRIXDNRXQ
YXARNRKKXAKEINFXERSN*kklknkk*k*nq*kkk*kknl

Frame B:
llaywikn*ltsfyngvlqliksqlefmlmvvli*cildmqmh*DKQGELGDILVVGVHT
DEEIAKNKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISV
GAD---

---ftsmlsfmvmiklxxvxkvvxihtnxqxn*vftxklnilkvxxqlkslkesliidxn
xkxxivkxkxkklislndxikkn*kiknknktnkkknrkki

Frame C:
cwptg*kin*qvftmeyyn**kan*slc*wlf*fnafwtckciktsken*eif**lvyiq
mkklqkikvhql*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifql
vq---

---shqccrsw**ssxtrsxrwcxsiqtpxrirylqxs*ty*rspxn*nr*knx***tsi
xsxxs*kxsxrn*fx*tixlkkikk*kikikpikkkiekkf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC294 (VFC294Q) /CSM/VF/VFC2-D/VFC294Q.Seq.d/                        995   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                        670   0.0
VFM413 (VFM413Q) /CSM/VF/VFM4-A/VFM413Q.Seq.d/                        668   0.0
CFG421 (CFG421Q) /CSM/CF/CFG4-A/CFG421Q.Seq.d/                        666   0.0
SFH515 (SFH515Q) /CSM/SF/SFH5-A/SFH515Q.Seq.d/                        664   0.0
VFL367 (VFL367Q) /CSM/VF/VFL3-C/VFL367Q.Seq.d/                        662   0.0
VFC392 (VFC392Q) /CSM/VF/VFC3-D/VFC392Q.Seq.d/                        652   0.0
VFO305 (VFO305Q) /CSM/VF/VFO3-A/VFO305Q.Seq.d/                        650   0.0
VFF663 (VFF663Q) /CSM/VF/VFF6-C/VFF663Q.Seq.d/                        650   0.0
VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                        650   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    72  1e-11  3
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    72  2e-11  3
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    72  2e-11  3
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    72  2e-11  3
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    72  3e-11  3
BG642411|BG642411.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end, mRNA sequence.    72  3e-11  3
BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP17G1 5' sequence, mRNA sequence.    72  3e-11  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    72  5e-11  3
AW625369|AW625369.1 EST319192 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ13A13 5', mRNA sequence.    68  3e-10  3
BG133537|BG133537.1 EST466525 tomato crown gall Lycopersicon esculentum cDNA clone cTOE13N15 5' sequence, mRNA sequence.    72  6e-10  2
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   159   3e-54
AY813109_1(AY813109|pid:none) Schistosoma japonicum SJCHGC06128 ...   100   3e-31
BT077764_1(BT077764|pid:none) Lepeophtheirus salmonis clone lsal...   103   9e-30
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   107   2e-29
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   107   2e-29
AL663030_11(AL663030|pid:none) Mouse DNA sequence from clone RP2...   107   2e-29
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   107   3e-29
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   103   3e-29
BC088018_1(BC088018|pid:none) Xenopus tropicalis phosphate cytid...   105   1e-28
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   103   3e-28
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.37  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.06  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFC294 is nuc
5' end seq. ID VFC294F
5' end seq. 
>VFC294F.Seq
ACTGTTGGCCTACTGGATAAAAAATTAATTAACAAGTTTTTACAATGGAGTACTACAACT
AATAAAAAGCCAATTAGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACAT
GCAAATGCATTAAGACAAGCAAGGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATAC
AGATGAAGAAATTGCAAAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAA
AGCAGTACGTGCATGTAAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAAC
TGAAGAATATTTGGATAGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGT
TGGTGCAGAT----------
Length of 5' end seq. 370
3' end seq. ID VFC294Z
3' end seq. 
>VFC294Z.Seq
----------CTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTGNACNCGGTCC
ANAAGGTGGTGTANATCCATACAAACNCCCAANAGAATTAGGTATTTACAAANAAGTTAA
ACATACTGAAGGTCNCCCNGCAACTGAAATCGTTAAAAGAATCNTTGATAATAGACNTCA
ATATNAAGCNCGNAATCGTAAAAAANAAGCNAAAGAAATTAATTTCNTTGAACGATCNAA
TTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACCAATAAAAAAAAAAATAGAAAAA
AAATTTA
Length of 3' end seq. 297
Connected seq. ID VFC294P
Connected seq. 
>VFC294P.Seq
ACTGTTGGCCTACTGGATAAAAAATTAATTAACAAGTTTTTACAATGGAGTACTACAACT
AATAAAAAGCCAATTAGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACAT
GCAAATGCATTAAGACAAGCAAGGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATAC
AGATGAAGAAATTGCAAAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAA
AGCAGTACGTGCATGTAAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAAC
TGAAGAATATTTGGATAGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGT
TGGTGCAGAT----------CTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTG
NACNCGGTCCANAAGGTGGTGTANATCCATACAAACNCCCAANAGAATTAGGTATTTACA
AANAAGTTAAACATACTGAAGGTCNCCCNGCAACTGAAATCGTTAAAAGAATCNTTGATA
ATAGACNTCAATATNAAGCNCGNAATCGTAAAAAANAAGCNAAAGAAATTAATTTCNTTG
AACGATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACCAATAAAAAAAAA
AATAGAAAAAAAATTTA
Length of connected seq. 667
Full length Seq ID -
Full length Seq. -
Length of full length seq. -