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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC274
Library VF
(Link to library)
Clone ID VFC274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFC274E
(Link to Original site)
Representative DNA sequence 
>VFC274 (VFC274Q) /CSM/VF/VFC2-D/VFC274Q.Seq.d/
CTGTTGGCCTACTGGNACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGNACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAA
ATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCC
TTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCAC
ACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAA
TTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTC
TTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCC
ATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGT
GGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCC
CCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATT
GGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTNCCAAAGAAGA
ATACGACGAATCAGGTCCATCAATTGTTNCACAGAAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
LLAYWXVLFSHQLLVVQXHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDD
MEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLS
LYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILTERGYSFT
TTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEAL
FQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALA
PSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIXQRRIRRIRSINCXTE
Translated Amino Acid sequence (All Frames) 
Frame A:
LLAYWXVLFSHQLLVVQXHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDD
MEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLS
LYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILTERGYSFT
TTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEAL
FQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALA
PSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIXQRRIRRIRSINCXTE


Frame B:
cwptgtccfpincwsskxtlvlwsvwvkkthm*vmkpnqrevs*psntqlntvslptgmi
wkksgiilstmnsvlpqkntqfs*lkph*iqrptekk*pklclkpstpqpctspfkpsyp
ymplvvppvllwiqvmvsptlyqsmkvmlyhmpsyv*i*lvvilpit**ks*lnvvthsp
plpkeksseiskkn*ptspstlklkckllphhqpsknhtnyqtvksspsvmnvsvaqkps
snhhs*vwnllvstkppttps*nvmlisvkiytvtssypvvqlcsqvlliv*tkn*ll*p
hqp*klksllhqnvntlsglvdls*lhsqlsnkcgsxkeeydesgpsivxqk


Frame C:
vgllxravfpsivgrpxthwcygrygskrlicr**spikerylnpqipn*tryryqlg*y
gknlasyflq*tpccprrtpssln*spikskgqqrkndpnyv*nlqhpshvrrhssrlil
iclwsyhrycygfr*wclphctnl*rlcfttchltfrfsws*syrlhdenln*twllihh
hcrkrnrqryqrkislrrprl*s*nanccliisprkiiritrrsshhhr**tfplprspl
ptiilrygicwyprnhlqlhhem*c*yp*rfir*rrlirwynyvpryc*syeqrincfsp
inhen*nhcstrt*ilcldwwiylsftlnfptnvdlpkknttnqvhqlxhrk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC274 (VFC274Q) /CSM/VF/VFC2-D/VFC274Q.Seq.d/                       2074   0.0
VFM814 (VFM814Q) /CSM/VF/VFM8-A/VFM814Q.Seq.d/                       2018   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       2018   0.0
VFM463 (VFM463Q) /CSM/VF/VFM4-C/VFM463Q.Seq.d/                       2018   0.0
VFL880 (VFL880Q) /CSM/VF/VFL8-D/VFL880Q.Seq.d/                       2018   0.0
VFL749 (VFL749Q) /CSM/VF/VFL7-C/VFL749Q.Seq.d/                       2018   0.0
VFL740 (VFL740Q) /CSM/VF/VFL7-B/VFL740Q.Seq.d/                       2018   0.0
VFL458 (VFL458Q) /CSM/VF/VFL4-C/VFL458Q.Seq.d/                       2018   0.0
VFL313 (VFL313Q) /CSM/VF/VFL3-A/VFL313Q.Seq.d/                       2018   0.0
VFK736 (VFK736Q) /CSM/VF/VFK7-B/VFK736Q.Seq.d/                       2018   0.0
own update 2001.12. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1895  0.0  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1784  0.0  3
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       1752  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1752  0.0  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1538  0.0  8
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.  1443  0.0  14
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1368  0.0  6
X03282|X03282.1 dictyostelium discoideum gene for actin A12.    1336  0.0  3
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1217  0.0  3
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.  1166  0.0  2
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   638   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   634   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   634   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   632   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        635   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   633   0.0
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   625   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   625   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          625   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   623   e-180
protein update 2009. 7.10
PSORT 

 psg: 0.92  gvh: 0.59  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFC274 is cyt
5' end seq. ID VFC274F
5' end seq. 
>VFC274F.Seq
CTGTTGGCCTACTGGNACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGNACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAA
ATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCC
TTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCAC
ACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCCTC----------
Length of 5' end seq. 632
3' end seq. ID VFC274Z
3' end seq. 
>VFC274Z.Seq
----------GATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCACA
TGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATCTT
AACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAA
AGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATC
ATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGA
ACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGG
TATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTT
ATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTAT
GAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACC
AGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCA
ACAAATGTGGATCTNCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTNCACAGA
AAA
Length of 3' end seq. 653
Connected seq. ID VFC274P
Connected seq. 
>VFC274P.Seq
CTGTTGGCCTACTGGNACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGNACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAA
ATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCC
TTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCAC
ACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCCTC----------GATGGTGTCTCCCACACT
GTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGT
CGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACC
ACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGAC
TTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTA
CCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTC
CAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATC
ATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGT
ACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCA
TCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGT
GGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTNCCAAAGAAGAATA
CGACGAATCAGGTCCATCAATTGTTNCACAGAAAA
Length of connected seq. 1285
Full length Seq ID VFC274E
Full length Seq. 
>VFC274E.Seq
CTGTTGGCCTACTGGNACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGNACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAA
ATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCC
TTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCAC
ACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAA
TTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTC
TTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCC
ATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGT
GGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCC
CCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATT
GGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTNCCAAAGAAGA
ATACGACGAATCAGGTCCATCAATTGTTNCACAGAAAA
Length of full length seq. 1058