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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC258
Library VF
(Link to library)
Clone ID VFC258
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13874-1
Original site URL
Representative seq. ID VFC258P
(Link to Original site)
Representative DNA sequence 
>VFC258 (VFC258Q) /CSM/VF/VFC2-C/VFC258Q.Seq.d/
TGTTGGCCTACTGGNATATCAGTGGACTATTCAATCATATAATAATGACAATTTTCATAG
NATTTAGCATCATTAGAAGGATTTATACAATGATTTAATCTCACATATTGAAACTTTAAG
AAAAAAGAAAGGTTCAGAATACTCTGTTGCTGATTATCAAATTTTAATTGGTGAACAACG
TACTGTTGAATTCTTATCAAAAATCGTTTCAAATGAAACTGAATTCTTATTCAAAGAGTG
TAAAGATTCAGATAAAGATATTGAAGGATTTTTTTAATGTTATTTTAACTATTTTACATA
AATTAGAAAATGAAGATGAAATCAATATTGTCTCACAAAAGATTAGAGGATTCATTATXX
XXXXXXXXAACAAAAGAATCCAAACTTCCTCTCAACCATTGGTCTCTCAAATGATGATTG
TCTTCAAAAGATTCGTCTTTTATCTTTGGCCACTTTAACCTCTGAACAAAGTAAAGTTCC
ATACAGTTTAATCTCAAAAATGCTTCAAATCGATGAAAATGAAGTTGAAATGTGGGTTAT
CAATGCAATGGAAGGTGATTTATTAGATGCCAAATTAGATCAATTAAATCGTATCGTTAA
TGTTAACTCATCAACTCAAAGAGTTTTCAATAAATCTCAATGGTCTCAATTAGGTCAACG
TTTCTCTGTTTGGAAATCAAGTGTTAAAAATTTACNCCAAGTTATCGAAAATGCTAAAAC
TACNCAAGTTAAACCATNCTATTNCCAAAC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
laywxisglfnhiimtifixfsiirriytmi*shilkl*ekrkvqntllliikf*LVNNV
LLNSYQKSFQMKLNSYSKSVKIQIKILKDFFNVILTILHKLENEDEINIVSQKIRGFI--
-

---QKNPNFLSTIGLSNDDCLQKIRLLSLATLTSEQSKVPYSLISKMLQIDENEVEMWVI
NAMEGDLLDAKLDQLNRIVNVNSSTQRVFNKSQWSQLGQRFSVWKSSVKNLXQVIENAKT
TQVKPXYXQ
Translated Amino Acid sequence (All Frames) 
Frame A:
cwptgisvdysii***qfs*xlaslegfiq*fnlty*nfkkkerfrilcc*lsnfnw*tt
yc*iliknrfk*n*iliqrv*rfr*ry*riflmlf*lfyin*kmkmksilshkrledsl-
--

---nkriqtssqplvsqmmivfkrfvfylwpl*plnkvkfhtv*sqkcfksmkmklkcgl
smqwkviy*mpn*in*ivslmlthqlkefsinlngln*vnvslfgnqvlkiyxklskmlk
lxklnhxixk

Frame B:
vgllxyqwtiqsynndnfhxi*hh*kdlyndlishietlrkkkgseysvadyqiligeqr
tveflskivsneteflfkeckdsdkdiegff*cyfnyft*irk*r*nqycltkd*rihy-
--

---tkesklplnhwslk**lsskdssfifghfnl*tk*ssiqfnlknasnr*k*s*nvgy
qcngr*fircqirsiksyr*c*linsksfq*ismvsirstflcleikc*kftpsyrkc*n
yxs*txlxpn

Frame C:
laywxisglfnhiimtifixfsiirriytmi*shilkl*ekrkvqntllliikf*LVNNV
LLNSYQKSFQMKLNSYSKSVKIQIKILKDFFNVILTILHKLENEDEINIVSQKIRGFI--
-

---QKNPNFLSTIGLSNDDCLQKIRLLSLATLTSEQSKVPYSLISKMLQIDENEVEMWVI
NAMEGDLLDAKLDQLNRIVNVNSSTQRVFNKSQWSQLGQRFSVWKSSVKNLXQVIENAKT
TQVKPXYXQ
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC258 (VFC258Q) /CSM/VF/VFC2-C/VFC258Q.Seq.d/                       1350   0.0
VFM633 (VFM633Q) /CSM/VF/VFM6-B/VFM633Q.Seq.d/                        733   0.0
VFH808 (VFH808Q) /CSM/VF/VFH8-A/VFH808Q.Seq.d/                        733   0.0
VFF606 (VFF606Q) /CSM/VF/VFF6-A/VFF606Q.Seq.d/                        733   0.0
VFC393 (VFC393Q) /CSM/VF/VFC3-D/VFC393Q.Seq.d/                        728   0.0
SSF703 (SSF703Q) /CSM/SS/SSF7-A/SSF703Q.Seq.d/                        726   0.0
AHP694 (AHP694Q) /CSM/AH/AHP6-D/AHP694Q.Seq.d/                        726   0.0
VFF373 (VFF373Q) /CSM/VF/VFF3-D/VFF373Q.Seq.d/                        720   0.0
AFI259 (AFI259Q) /CSM/AF/AFI2-C/AFI259Q.Seq.d/                        720   0.0
VFG289 (VFG289Q) /CSM/VF/VFG2-D/VFG289Q.Seq.d/                        662   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC139304|AC139304.1 Pongo pygmaeus clone CH253-23J2, WORKING DRAFT SEQUENCE, 12 unordered pieces.    46  0.076  5
AC092685|AC092685.2 Homo sapiens BAC clone RP11-746P2 from 7, complete sequence.    48  0.16  1
BZ969150|BZ969150.1 PUFIC79TD ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa348N13, DNA sequence.    36  0.20  2
AC094106|AC094106.3 Homo sapiens chromosome 5 clone RP11-474J18, complete sequence.    46  0.21  4
AC141590|AC141590.1 Homo sapiens chromosome 5 clone RP11-1068D20, WORKING DRAFT SEQUENCE, 2 unordered pieces.    46  0.31  4
AC127493|AC127493.4 Homo sapiens chromosome 18 clone RP11-539C8 map 18, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    46  0.62  1
AQ182602|AQ182602.1 HS_3094_B1_B07_MF CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3094 Col=13 Row=D, genomic survey sequence.    46  0.62  1
CC154672|CC154672.1 CSU-K34.102E24.SP6.1 CSU-K34 Aedes aegypti genomic clone CSU-K34-102E24, DNA sequence.    46  0.62  1
AF097649|AF097649.1 Homo sapiens chromosome 7qtelo BAC E5, complete sequence.    46  0.64  2
AL390722|AL390722.10 Human DNA sequence from clone RP11-474J1 on chromosome 13.    46  0.83  3
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KZ8) RecName: Full=Eukaryotic translation initiation factor ...   242   1e-62
AY062722_1(AY062722|pid:none) Arabidopsis thaliana Unknown prote...   110   6e-23
AK227983_1(AK227983|pid:none) Arabidopsis thaliana mRNA for hypo...   106   7e-22
EF147928_1(EF147928|pid:none) Populus trichocarpa clone WS0126_A...   102   1e-20
FB863365_1(FB863365|pid:none) Sequence 82638 from Patent WO20080...   102   1e-20
DQ235192_1(DQ235192|pid:none) Solanum tuberosum clone 167F07 unk...   101   2e-20
EF678701_1(EF678701|pid:none) Picea sitchensis clone WS0294_G09 ...   100   6e-20
EF678031_1(EF678031|pid:none) Picea sitchensis clone WS0281_M23 ...    99   1e-19
FB863381_1(FB863381|pid:none) Sequence 82654 from Patent WO20080...    99   1e-19
FB863367_1(FB863367|pid:none) Sequence 82640 from Patent WO20080...    93   1e-17
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.31  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VFC258 is cyt
5' end seq. ID VFC258F
5' end seq. 
>VFC258F.Seq
TGTTGGCCTACTGGNATATCAGTGGACTATTCAATCATATAATAATGACAATTTTCATAG
NATTTAGCATCATTAGAAGGATTTATACAATGATTTAATCTCACATATTGAAACTTTAAG
AAAAAAGAAAGGTTCAGAATACTCTGTTGCTGATTATCAAATTTTAATTGGTGAACAACG
TACTGTTGAATTCTTATCAAAAATCGTTTCAAATGAAACTGAATTCTTATTCAAAGAGTG
TAAAGATTCAGATAAAGATATTGAAGGATTTTTTTAATGTTATTTTAACTATTTTACATA
AATTAGAAAATGAAGATGAAATCAATATTGTCTCACAAAAGATTAGAGGATTCATTAT--
--------
Length of 5' end seq. 358
3' end seq. ID VFC258Z
3' end seq. 
>VFC258Z.Seq
----------AACAAAAGAATCCAAACTTCCTCTCAACCATTGGTCTCTCAAATGATGAT
TGTCTTCAAAAGATTCGTCTTTTATCTTTGGCCACTTTAACCTCTGAACAAAGTAAAGTT
CCATACAGTTTAATCTCAAAAATGCTTCAAATCGATGAAAATGAAGTTGAAATGTGGGTT
ATCAATGCAATGGAAGGTGATTTATTAGATGCCAAATTAGATCAATTAAATCGTATCGTT
AATGTTAACTCATCAACTCAAAGAGTTTTCAATAAATCTCAATGGTCTCAATTAGGTCAA
CGTTTCTCTGTTTGGAAATCAAGTGTTAAAAATTTACNCCAAGTTATCGAAAATGCTAAA
ACTACNCAAGTTAAACCATNCTATTNCCAAAC
Length of 3' end seq. 382
Connected seq. ID VFC258P
Connected seq. 
>VFC258P.Seq
TGTTGGCCTACTGGNATATCAGTGGACTATTCAATCATATAATAATGACAATTTTCATAG
NATTTAGCATCATTAGAAGGATTTATACAATGATTTAATCTCACATATTGAAACTTTAAG
AAAAAAGAAAGGTTCAGAATACTCTGTTGCTGATTATCAAATTTTAATTGGTGAACAACG
TACTGTTGAATTCTTATCAAAAATCGTTTCAAATGAAACTGAATTCTTATTCAAAGAGTG
TAAAGATTCAGATAAAGATATTGAAGGATTTTTTTAATGTTATTTTAACTATTTTACATA
AATTAGAAAATGAAGATGAAATCAATATTGTCTCACAAAAGATTAGAGGATTCATTAT--
--------AACAAAAGAATCCAAACTTCCTCTCAACCATTGGTCTCTCAAATGATGATTG
TCTTCAAAAGATTCGTCTTTTATCTTTGGCCACTTTAACCTCTGAACAAAGTAAAGTTCC
ATACAGTTTAATCTCAAAAATGCTTCAAATCGATGAAAATGAAGTTGAAATGTGGGTTAT
CAATGCAATGGAAGGTGATTTATTAGATGCCAAATTAGATCAATTAAATCGTATCGTTAA
TGTTAACTCATCAACTCAAAGAGTTTTCAATAAATCTCAATGGTCTCAATTAGGTCAACG
TTTCTCTGTTTGGAAATCAAGTGTTAAAAATTTACNCCAAGTTATCGAAAATGCTAAAAC
TACNCAAGTTAAACCATNCTATTNCCAAAC
Length of connected seq. 740
Full length Seq ID -
Full length Seq. -
Length of full length seq. -