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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC253
Library VF
(Link to library)
Clone ID VFC253
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12804-1
Original site URL
Representative seq. ID VFC253E
(Link to Original site)
Representative DNA sequence 
>VFC253 (VFC253Q) /CSM/VF/VFC2-C/VFC253Q.Seq.d/
ACTGTTGGCCTACTGGNTTCATNACTCAACTTTCTNCTCTTNACAAATGTTAATATTNCC
AACAAATTGTACAATTGTAAAAATAGAAAATGGATGATGATGATGATGATGACGATGAAA
AGGTATAAAGNATGAGGNAAGATGNAAATTAAAACTGATAATTTAGAACAACAAGTTAAT
GATCATGATGATGACGATGATGATGTTCCATATGGTCCAAAATCATTTGATAAGGAATTT
ACTTTACAAACTATTGATACTAAAAAACATCCAAAAGTATTAAAAACTTTTACAATTAAT
ATTCAAGTAACAAATACAGATAAAGATGGTATTATAACAAAATCAAATAATGTCATTTGG
AAATATAGTAAAGATAAATTAGCAGAATCCCTATAAATACACCAATAAAGTTGATGGTGT
TGAATTATGTTTCACCGATTCATCAATGAATATTGCAAACATTTCATTGAATGGTAGTAA
ATCAGATGGTCCATTTTCAAATGCTTATTATAACAAATACTTCTTTGGTGATAATTCAAT
TGCAGGATTATTCACTGATCAATCCTATTTGAATCAATTTACAATTTCAACCACCTATAC
AAACATTGATGGATTAAATTTAACTACATTATCAAAAGAAAGTGATAATTCAAATGTTTA
TTACTTCCCAGCCGGTTGGAGTTTCGAAACTTTTGATACCACAAACACTGACCTTTCCAC
TCCATTATTAATTACAAAGGTAAATAATGGTTCAACTGAAACTTGGAGAATCATTAAATA
TGATAACACTGTCGATAGTGATTTATACTCTAAATGTCAACCACCAAAGAAAGGAGGAAT
TCATGTTAATATCTCCAAAAATGGTATCATTGCCGTTGTCGTCATTGTTGTCGTTGTTAT
TATTGGTGTTGGTGCTATCGTTTTTATAGTCATTAAAAAGAGAGGTGGTNCTTGGTAAAA
AAATGCCTGCTCAC
sequence update 2001. 6. 9
Translated Amino Acid sequence 
llaywxhxstfxsxqmlixptnctivkieng******r*kgikxexrxklklii*nnklm
immmtmmmfhmvqnhlirnllykllilkniqky*kllqlifk*qiqikmvl*qnqimsfg
nivkin*QNPYKYTNKVDGVELCFTDSSMNIANISLNGSKSDGPFSNAYYNKYFFGDNSI
AGLFTDQSYLNQFTISTTYTNIDGLNLTTLSKESDNSNVYYFPAGWSFETFDTTNTDLST
PLLITKVNNGSTETWRIIKYDNTVDSDLYSKCQPPKKGGIHVNISKNGIIAVVVIVVVVI
IGVGAIVFIVIKKRGGXW*knacs
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxsxlnfllxtnvnixnklyncknrkwmmmmmmtmkrykx*gkmxiktdnleqqvn
dhddddddvpygpksfdkeftlqtidtkkhpkvlktftiniqvtntdkdgiitksnnviw
kyskdklaesl*ihq*s*wc*imfhrfineyckhfiew**irwsifkcll*qillw**fn
criih*silfesiynfnhlykh*wikfnyiikrk**fkclllpsrlefrnf*yhkh*pfh
siinykgk*wfn*nlenh*i**hcr**fil*msttkerrnsc*ylqkwyhcrcrhccrcy
ywcwcyrfysh*kerwxlvkkcll


Frame B:
llaywxhxstfxsxqmlixptnctivkieng******r*kgikxexrxklklii*nnklm
immmtmmmfhmvqnhlirnllykllilkniqky*kllqlifk*qiqikmvl*qnqimsfg
nivkin*QNPYKYTNKVDGVELCFTDSSMNIANISLNGSKSDGPFSNAYYNKYFFGDNSI
AGLFTDQSYLNQFTISTTYTNIDGLNLTTLSKESDNSNVYYFPAGWSFETFDTTNTDLST
PLLITKVNNGSTETWRIIKYDNTVDSDLYSKCQPPKKGGIHVNISKNGIIAVVVIVVVVI
IGVGAIVFIVIKKRGGXW*knacs


Frame C:
cwptgfxtqlsxlxkc*yxqqivql*k*kmddddddddekv*xmrxdxn*n**frtts**
s***r**csiwskii**giyftny*y*ktsksiknfyn*yssnkyr*rwyynkik*chle
i**r*isripintpiklmvlnyvspihq*ilqtfh*mvvnqmvhfqmliitntslviiql
qdyslinpi*inlqfqppiqtlmd*i*lhyqkkviiqmfitsqpvgvskllipqtltfpl
hy*lqr*imvqlklgeslnmitlsiviytlnvnhqrkeefmlispkmvslplssllslll
lvlvlsfl*slkrevvlgkkmpah
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC253 (VFC253Q) /CSM/VF/VFC2-C/VFC253Q.Seq.d/                       1483   0.0
VHP795 (VHP795Q) /CSM/VH/VHP7-D/VHP795Q.Seq.d/                       1338   0.0
VHN487 (VHN487Q) /CSM/VH/VHN4-D/VHN487Q.Seq.d/                       1316   0.0
VHJ180 (VHJ180Q) /CSM/VH/VHJ1-D/VHJ180Q.Seq.d/                       1257   0.0
AHO295 (AHO295Q) /CSM/AH/AHO2-D/AHO295Q.Seq.d/                       1257   0.0
VHI281 (VHI281Q) /CSM/VH/VHI2-D/VHI281Q.Seq.d/                       1187   0.0
VHE114 (VHE114Q) /CSM/VH/VHE1-A/VHE114Q.Seq.d/                       1187   0.0
AFL483 (AFL483Q) /CSM/AF/AFL4-D/AFL483Q.Seq.d/                       1179   0.0
VHK874 (VHK874Q) /CSM/VH/VHK8-D/VHK874Q.Seq.d/                       1174   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX489330|AX489330.1 Sequence 6630 from Patent WO02053728.         56  8e-04  1
AC136652|AC136652.1 Rattus norvegicus clone CH230-359N16, *** SEQUENCING IN PROGRESS ***, 46 unordered pieces.    52  0.013  1
AE014837|AE014837.1 Plasmodium falciparum 3D7 chromosome 11 section 2 of 8 of the complete sequence.    34  0.041  9
AL929355|AL929355.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 1/5.    34  0.061  9
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    42  0.11  10
AC020597|AC020597.4 Homo sapiens chromosome 11 clone RP11-390G21, WORKING DRAFT SEQUENCE, 22 unordered pieces.    40  0.32  3
AC119464|AC119464.5 Rattus norvegicus clone CH230-329N4, WORKING DRAFT SEQUENCE, 4 unordered pieces.    40  0.47  4
AC091126|AC091126.1 Drosophila melanogaster clone RP98-25M5, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    42  0.55  4
AL035477|AL035477.6 Plasmodium falciparum MAL4P4.                 38  0.71  6
BX550080|BX550080.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse110g09_p1c.    46  0.82  1
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116957_18(AC116957|pid:none) Dictyostelium discoideum chromoso...    40   0.12
AE014188_55(AE014188|pid:none) Plasmodium falciparum 3D7 chromos...    38   0.46
AL034559_46(AL034559|pid:none) Plasmodium falciparum MAL3P7, com...    37   1.0
AY181249_4(AY181249|pid:none) Candida glabrata mating pheromone ...    35   5.1
CR767821_285(CR767821|pid:none) Ehrlichia ruminantium strain Wel...    35   5.1
BVEBCZ(A28181) cysZ protein - Salmonella typhimurium  &M21450_1(...    34   6.6
CP000886_516(CP000886|pid:none) Salmonella enterica subsp. enter...    34   6.6
CP001184_591(CP001184|pid:none) Ureaplasma urealyticum serovar 1...    34   8.7
protein update 2009. 7.10
PSORT 

 psg: 0.71  gvh: 0.34  alm: 0.05  top: 0.50  tms: 0.07  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: plasma membrane
	  8.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VFC253 is cyt
5' end seq. ID VFC253F
5' end seq. 
>VFC253F.Seq
ACTGTTGGCCTACTGGNTTCATNACTCAACTTTCTNCTCTTNACAAATGTTAATATTNCC
AACAAATTGTACAATTGTAAAAATAGAAAATGGATGATGATGATGATGATGACGATGAAA
AGGTATAAAGNATGAGGNAAGATGNAAATTAAAACTGATAATTTAGAACAACAAGTTAAT
GATCATGATGATGACGATGATGATGTTCCATATGGTCCAAAATCATTTGATAAGGAATTT
ACTTTACAAACTATTGATACTAAAAAACATCCAAAAGTATTAAAAACTTTTACAATTAAT
ATTCAAGTAACAAATACAGATAAAGATGGTATTATAACAAAATCAAATAATGTCATTTGG
AAATATAGTAAAGATAAATTAGCAGAATCCTATAAATACACCAATAAAGNTGATGGTGTN
GAATTATGTTTCACCGATTCATCAATGAATATTGCAAACATTTCATTGAATGGTAGTAAA
----------
Length of 5' end seq. 480
3' end seq. ID VFC253Z
3' end seq. 
>VFC253Z.Seq
----------ANTGTCATTTGGAAATATAGTAAAGATAAATTAGCAGAATCCCTATAAAT
ACACCAATAAAGTTGATGGTGTTGAATTATGTTTCACCGATTCATCAATGAATATTGCAA
ACATTTCATTGAATGGTAGTAAATCAGATGGTCCATTTTCAAATGCTTATTATAACAAAT
ACTTCTTTGGTGATAATTCAATTGCAGGATTATTCACTGATCAATCCTATTTGAATCAAT
TTACAATTTCAACCACCTATACAAACATTGATGGATTAAATTTAACTACATTATCAAAAG
AAAGTGATAATTCAAATGTTTATTACTTCCCAGCCGGTTGGAGTTTCGAAACTTTTGATA
CCACAAACACTGACCTTTCCACTCCATTATTAATTACAAAGGTAAATAATGGTTCAACTG
AAACTTGGAGAATCATTAAATATGATAACACTGTCGATAGTGATTTATACTCTAAATGTC
AACCACCAAAGAAAGGAGGAATTCATGTTAATATCTCCAAAAATGGTATCATTGCCGTTG
TCGTCATTGTTGTCGTTGTTATTATTGGTGTTGGTGCTATCGTTTTTATAGTCATTAAAA
AGAGAGGTGGTNCTTGGTAAAAAAATGCCTGCTCAC
Length of 3' end seq. 626
Connected seq. ID VFC253P
Connected seq. 
>VFC253P.Seq
ACTGTTGGCCTACTGGNTTCATNACTCAACTTTCTNCTCTTNACAAATGTTAATATTNCC
AACAAATTGTACAATTGTAAAAATAGAAAATGGATGATGATGATGATGATGACGATGAAA
AGGTATAAAGNATGAGGNAAGATGNAAATTAAAACTGATAATTTAGAACAACAAGTTAAT
GATCATGATGATGACGATGATGATGTTCCATATGGTCCAAAATCATTTGATAAGGAATTT
ACTTTACAAACTATTGATACTAAAAAACATCCAAAAGTATTAAAAACTTTTACAATTAAT
ATTCAAGTAACAAATACAGATAAAGATGGTATTATAACAAAATCAAATAATGTCATTTGG
AAATATAGTAAAGATAAATTAGCAGAATCCTATAAATACACCAATAAAGNTGATGGTGTN
GAATTATGTTTCACCGATTCATCAATGAATATTGCAAACATTTCATTGAATGGTAGTAAA
----------ANTGTCATTTGGAAATATAGTAAAGATAAATTAGCAGAATCCCTATAAAT
ACACCAATAAAGTTGATGGTGTTGAATTATGTTTCACCGATTCATCAATGAATATTGCAA
ACATTTCATTGAATGGTAGTAAATCAGATGGTCCATTTTCAAATGCTTATTATAACAAAT
ACTTCTTTGGTGATAATTCAATTGCAGGATTATTCACTGATCAATCCTATTTGAATCAAT
TTACAATTTCAACCACCTATACAAACATTGATGGATTAAATTTAACTACATTATCAAAAG
AAAGTGATAATTCAAATGTTTATTACTTCCCAGCCGGTTGGAGTTTCGAAACTTTTGATA
CCACAAACACTGACCTTTCCACTCCATTATTAATTACAAAGGTAAATAATGGTTCAACTG
AAACTTGGAGAATCATTAAATATGATAACACTGTCGATAGTGATTTATACTCTAAATGTC
AACCACCAAAGAAAGGAGGAATTCATGTTAATATCTCCAAAAATGGTATCATTGCCGTTG
TCGTCATTGTTGTCGTTGTTATTATTGGTGTTGGTGCTATCGTTTTTATAGTCATTAAAA
AGAGAGGTGGTNCTTGGTAAAAAAATGCCTGCTCAC
Length of connected seq. 1106
Full length Seq ID VFC253E
Full length Seq. 
>VFC253E.Seq
ACTGTTGGCCTACTGGNTTCATNACTCAACTTTCTNCTCTTNACAAATGTTAATATTNCC
AACAAATTGTACAATTGTAAAAATAGAAAATGGATGATGATGATGATGATGACGATGAAA
AGGTATAAAGNATGAGGNAAGATGNAAATTAAAACTGATAATTTAGAACAACAAGTTAAT
GATCATGATGATGACGATGATGATGTTCCATATGGTCCAAAATCATTTGATAAGGAATTT
ACTTTACAAACTATTGATACTAAAAAACATCCAAAAGTATTAAAAACTTTTACAATTAAT
ATTCAAGTAACAAATACAGATAAAGATGGTATTATAACAAAATCAAATAATGTCATTTGG
AAATATAGTAAAGATAAATTAGCAGAATCCCTATAAATACACCAATAAAGTTGATGGTGT
TGAATTATGTTTCACCGATTCATCAATGAATATTGCAAACATTTCATTGAATGGTAGTAA
ATCAGATGGTCCATTTTCAAATGCTTATTATAACAAATACTTCTTTGGTGATAATTCAAT
TGCAGGATTATTCACTGATCAATCCTATTTGAATCAATTTACAATTTCAACCACCTATAC
AAACATTGATGGATTAAATTTAACTACATTATCAAAAGAAAGTGATAATTCAAATGTTTA
TTACTTCCCAGCCGGTTGGAGTTTCGAAACTTTTGATACCACAAACACTGACCTTTCCAC
TCCATTATTAATTACAAAGGTAAATAATGGTTCAACTGAAACTTGGAGAATCATTAAATA
TGATAACACTGTCGATAGTGATTTATACTCTAAATGTCAACCACCAAAGAAAGGAGGAAT
TCATGTTAATATCTCCAAAAATGGTATCATTGCCGTTGTCGTCATTGTTGTCGTTGTTAT
TATTGGTGTTGGTGCTATCGTTTTTATAGTCATTAAAAAGAGAGGTGGTNCTTGGTAAAA
AAATGCCTGCTCAC
Length of full length seq. 974