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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC247
Library VF
(Link to library)
Clone ID VFC247
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16444-1
Original site URL
Representative seq. ID VFC247P
(Link to Original site)
Representative DNA sequence 
>VFC247 (VFC247Q) /CSM/VF/VFC2-B/VFC247Q.Seq.d/
GATATTTTCTAAAAAAATAAAAATAAAAATGGAAATGGAAGGATTTGATCATGTTACATT
TTGGGTTGGTAATGCATTACAAGCAGCAACTTATTACATTGCTAGATTTGGATTTCAAAA
TTTAGCTTATAGTGGTTTAGAAACTGGTAATCGTCAATTCGCCACTCATGTTATCCATCA
AAACAACATTATTATGGCTTTTACATCACCATTAACTGGTGACAACAAAGATTATGCAGA
CCACATGATGAGACATGGTGATGGTGTCAAAGATATTGCTTTCAACGTTAAAGATGTACA
ACACATTTATGATGAAGCAGTTAAAGCAGGAGCTCAATCAGTTAAAGAACCACXXXXXXX
XXXCCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTGAAA
AAGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAGAAT
ACGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACATCA
TTGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAACAT
ACTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATTTGG
ACACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTTTTACAAA
TCTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAAACA
ACCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGACAAC
AAGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATTATTAACTA
AATAACATTACAATATATCAAAATAAATAATAAATAAATACNCTATTAAAAAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
IFSKKIKIKMEMEGFDHVTFWVGNALQAATYYIARFGFQNLAYSGLETGNRQFATHVIHQ
NNIIMAFTSPLTGDNKDYADHMMRHGDGVKDIAFNVKDVQHIYDEAVKAGAQSVKEP---

---IHTEYSSLRSVVVADKSEKVKLPINEPANGIRKSQIQEYVDFYNGAGVQHIALKTDN
IIDAISKLRSRGVSFLTVPKTYYTSLREKLQHSSLEIKEDLDTLEKLHILIDYDDKGYLL
QIFTNNVEDKPTVFFEIIQRNNHDGFGAGNFKSLFEAIERQQETRGNL*vclnykcslll
tk*hynisk*iinkyxikk
Translated Amino Acid sequence (All Frames) 
Frame A:
dif*knknkngngri*scyilgw*citssnllhc*iwiskfsl*wfrnw*ssirhscyps
kqhyygfyitinw*qqrlcrphdetw*wcqrycfqr*rctthl**ss*srssis*rt---

---pfipnihh*dqs*llislkklnyqlmnqpmvlervkfknt*istmvlvfnisp*rli
tslmlsqn*dlvvslsslfqkhtihhsernyntlh*klkkiwtl*rnytf*simmtkvif
ykslqimlkinqlfslklskettmmvsvlvtlnpslkqskdnkklvetyrcvsitnvlyy
*lnnitiyqnk**intllk

Frame B:
IFSKKIKIKMEMEGFDHVTFWVGNALQAATYYIARFGFQNLAYSGLETGNRQFATHVIHQ
NNIIMAFTSPLTGDNKDYADHMMRHGDGVKDIAFNVKDVQHIYDEAVKAGAQSVKEP---

---hsyrifiikisrsc**v*ks*itn**tsqwy*kesnsrirrflqwcwcstyrlkd**
hh*cylkikiswclfphcsknilyitqreittlfirn*rrfghfreithfnrl**qrlsf
tnlyk*c*r*tncfl*nypkkqp*wfrcw*l*ipl*snrkttrnswkligvsqlqmffii
n*itlqyikinnk*ixy*k

Frame C:
yflkk*k*kwkwkdlimlhfglvmhykqqlitlldldfki*livv*klvivnsplmlsik
ttllwllhhh*lvttkimqtt**dmvmvskillstlkmyntfmmkqlkqelnqlknh---

---IHTEYSSLRSVVVADKSEKVKLPINEPANGIRKSQIQEYVDFYNGAGVQHIALKTDN
IIDAISKLRSRGVSFLTVPKTYYTSLREKLQHSSLEIKEDLDTLEKLHILIDYDDKGYLL
QIFTNNVEDKPTVFFEIIQRNNHDGFGAGNFKSLFEAIERQQETRGNL*vclnykcslll
tk*hynisk*iinkyxikk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH226 (VSH226Q) /CSM/VS/VSH2-B/VSH226Q.Seq.d/                       1049   0.0
VSD101 (VSD101Q) /CSM/VS/VSD1-A/VSD101Q.Seq.d/                       1049   0.0
VSB809 (VSB809Q) /CSM/VS/VSB8-A/VSB809Q.Seq.d/                       1049   0.0
VFO548 (VFO548Q) /CSM/VF/VFO5-B/VFO548Q.Seq.d/                       1049   0.0
VFO488 (VFO488Q) /CSM/VF/VFO4-D/VFO488Q.Seq.d/                       1049   0.0
VFN489 (VFN489Q) /CSM/VF/VFN4-D/VFN489Q.Seq.d/                       1049   0.0
VFN484 (VFN484Q) /CSM/VF/VFN4-D/VFN484Q.Seq.d/                       1049   0.0
VFN348 (VFN348Q) /CSM/VF/VFN3-B/VFN348Q.Seq.d/                       1049   0.0
VFN241 (VFN241Q) /CSM/VF/VFN2-B/VFN241Q.Seq.d/                       1049   0.0
VFM674 (VFM674Q) /CSM/VF/VFM6-D/VFM674Q.Seq.d/                       1049   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116978|AC116978.1 Dictyostelium discoideum chromosome 2 map 4846697-4874480 strain AX4, *** SEQUENCING IN PROGRESS ***.  1049  0.0  3
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.  1049  0.0  6
BI322343|BI322343.1 kx19h02.y3 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    42  5e-10  4
BM395514|BM395514.1 50072-2-9-E12.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    64  3e-06  1
BI502176|BI502176.1 kt86c08.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    36  8e-05  4
M59429|M59429.1 T. thermophila F-antigen (tfa) gene, complete cds.    46  6e-04  3
AC118979|AC118979.7 Rattus norvegicus clone CH230-313J16, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    50  0.051  1
AF410175|AF410175.1 Streptococcus thermophilus putative purine-nucleotide phosphorylase (deoD) gene, partial cds; insertion sequence isoIS1191 transposase, eps type I operon and insertion sequence isoIS1193 transposase genes, complete cds; and Orf14.9 gene, partial cds.    48  0.20  1
AX283711|AX283711.1 Sequence 5 from Patent WO0179500.             48  0.20  1
AX283719|AX283719.1 Sequence 13 from Patent WO0179500.            48  0.20  1
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NV5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   328   2e-88
AM114773_1(AM114773|pid:none) Platynereis dumerilii partial mRNA...   241   2e-62
AE014296_3563(AE014296|pid:none) Drosophila melanogaster chromos...   233   6e-60
S32821(S32821;S35890;S35889)4-hydroxyphenylpyruvate dioxygenase ...   232   2e-59
BC046075_1(BC046075|pid:none) Danio rerio zgc:56326, mRNA (cDNA ...   232   2e-59
(Q02110) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   232   2e-59
(Q6TGZ5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   232   2e-59
(P32754) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   231   2e-59
(Q5EA20) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   230   5e-59
BC077167_1(BC077167|pid:none) Danio rerio 4-hydroxyphenylpyruvat...   226   7e-58
protein update 2009. 6.17
PSORT 

 psg: 0.67  gvh: 0.43  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal

>> prediction for VFC247 is cyt
5' end seq. ID VFC247F
5' end seq. 
>VFC247F.Seq
GATATTTTCTAAAAAAATAAAAATAAAAATGGAAATGGAAGGATTTGATCATGTTACATT
TTGGGTTGGTAATGCATTACAAGCAGCAACTTATTACATTGCTAGATTTGGATTTCAAAA
TTTAGCTTATAGTGGTTTAGAAACTGGTAATCGTCAATTCGCCACTCATGTTATCCATCA
AAACAACATTATTATGGCTTTTACATCACCATTAACTGGTGACAACAAAGATTATGCAGA
CCACATGATGAGACATGGTGATGGTGTCAAAGATATTGCTTTCAACGTTAAAGATGTACA
ACACATTTATGATGAAGCAGTTAAAGCAGGAGCTCAATCAGTTAAAGAACCAC-------
---
Length of 5' end seq. 353
3' end seq. ID VFC247Z
3' end seq. 
>VFC247Z.Seq
----------CCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAG
TCTGAAAAAGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATT
CAAGAATACGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGAT
AACATCATTGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCA
AAAACATACTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAA
GATTTGGACACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTT
TTACAAATCTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAA
AGAAACAACCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAA
AGACAACAAGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATTA
TTAACTAAATAACATTACAATATATCAAAATAAATAATAAATAAATACNCTATTAAAAAG
Length of 3' end seq. 590
Connected seq. ID VFC247P
Connected seq. 
>VFC247P.Seq
GATATTTTCTAAAAAAATAAAAATAAAAATGGAAATGGAAGGATTTGATCATGTTACATT
TTGGGTTGGTAATGCATTACAAGCAGCAACTTATTACATTGCTAGATTTGGATTTCAAAA
TTTAGCTTATAGTGGTTTAGAAACTGGTAATCGTCAATTCGCCACTCATGTTATCCATCA
AAACAACATTATTATGGCTTTTACATCACCATTAACTGGTGACAACAAAGATTATGCAGA
CCACATGATGAGACATGGTGATGGTGTCAAAGATATTGCTTTCAACGTTAAAGATGTACA
ACACATTTATGATGAAGCAGTTAAAGCAGGAGCTCAATCAGTTAAAGAACCAC-------
---CCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTGAAA
AAGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAGAAT
ACGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACATCA
TTGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAACAT
ACTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATTTGG
ACACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTTTTACAAA
TCTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAAACA
ACCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGACAAC
AAGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATTATTAACTA
AATAACATTACAATATATCAAAATAAATAATAAATAAATACNCTATTAAAAAG
Length of connected seq. 943
Full length Seq ID -
Full length Seq. -
Length of full length seq. -