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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC238
Library VF
(Link to library)
Clone ID VFC238
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFC238P
(Link to Original site)
Representative DNA sequence 
>VFC238 (VFC238Q) /CSM/VF/VFC2-B/VFC238Q.Seq.d/
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAATAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCAXXXXXXXXXXTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCAC
GAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGT
AATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAA
GAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCCCCACCAGAACGT
AAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTC
TAAACAAACAATTAAAACTAGTGATGAAAGTGCTTCCTCACAAACAATTATGTAAAATAT
ATAATAAAATACATTATTTAATCATTTTTATTAATTTGTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllxf**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRH
TGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE
HP---

---FQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELT
ALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tn
n*n***kcfltnnyvkyiikyii*sfllic
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxf**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRH
TGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE
HP---

---ssnhhs*vwnqlvstkppttps*nvmlisvkiytvmssylvvqlcsqvlliv*tkn*
ll*phqp*klkslphqnvntlsglvdls*lhsqlsnkcgspkknmtnqvhqlstenvskq
tiktsdesassqtim*ni**ntlfnhfy*fv

Frame B:
llaywxfnkqifkyntiikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdt
lvlwsvwvkktlt*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvllqkn
t---

---lptiilrygiswyprnhlqlhhem*c*yp*rfir*crliwwynyvpryc*syeqrin
cfspinhen*nhcptrt*ilcldwwiylsftlnfptnvdlqrri*rirsincpqkmflnk
qlklvmkvlphkqlckiynkihyliifinlf

Frame C:
cwptgvlinkylniiq**ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskth
wcygrygskrllrr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccsrrt
p---

---FQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELT
ALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tn
n*n***kcfltnnyvkyiikyii*sfllic
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC238 (VFC238Q) /CSM/VF/VFC2-B/VFC238Q.Seq.d/                       1562   0.0
VFE354 (VFE354Q) /CSM/VF/VFE3-C/VFE354Q.Seq.d/                        866   0.0
VFN575 (VFN575Q) /CSM/VF/VFN5-D/VFN575Q.Seq.d/                        858   0.0
VFN265 (VFN265Q) /CSM/VF/VFN2-C/VFN265Q.Seq.d/                        858   0.0
VFM523 (VFM523Q) /CSM/VF/VFM5-A/VFM523Q.Seq.d/                        858   0.0
VFM262 (VFM262Q) /CSM/VF/VFM2-C/VFM262Q.Seq.d/                        858   0.0
VFI647 (VFI647Q) /CSM/VF/VFI6-B/VFI647Q.Seq.d/                        858   0.0
VFI453 (VFI453Q) /CSM/VF/VFI4-C/VFI453Q.Seq.d/                        858   0.0
VFH660 (VFH660Q) /CSM/VF/VFH6-C/VFH660Q.Seq.d/                        858   0.0
VFH302 (VFH302Q) /CSM/VF/VFH3-A/VFH302Q.Seq.d/                        858   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   753  0.0  5
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      640  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   624  0.0  7
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        618  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   618  0.0  7
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   539  0.0  11
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   507  0.0  6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   486  0.0  5
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     513  0.0  2
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   414  0.0  2
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A61043(A61043)actin CA15 - sea squirt (Styela clava) (fragments)      380   e-104
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   234   3e-60
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   234   3e-60
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   234   3e-60
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        234   3e-60
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   233   6e-60
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   232   1e-59
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   231   2e-59
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   230   4e-59
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   229   1e-58
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFC238 is nuc
5' end seq. ID VFC238F
5' end seq. 
>VFC238F.Seq
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAATAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCA----------
Length of 5' end seq. 366
3' end seq. ID VFC238Z
3' end seq. 
>VFC238Z.Seq
----------TCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACC
ACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTC
GTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTA
ACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCCCCACCAGAACGTAAATAC
TCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATC
TCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACA
AACAATTAAAACTAGTGATGAAAGTGCTTCCTCACAAACAATTATGTAAAATATATAATA
AAATACATTATTTAATCATTTTTATTAATTTGTTT
Length of 3' end seq. 445
Connected seq. ID VFC238P
Connected seq. 
>VFC238P.Seq
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAATAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCA----------TCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCAC
GAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGT
AATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAA
GAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCCCCACCAGAACGT
AAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTC
TAAACAAACAATTAAAACTAGTGATGAAAGTGCTTCCTCACAAACAATTATGTAAAATAT
ATAATAAAATACATTATTTAATCATTTTTATTAATTTGTTT
Length of connected seq. 811
Full length Seq ID -
Full length Seq. -
Length of full length seq. -