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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM221
Library AF
(Link to library)
Clone ID AFM221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM221P
(Link to Original site)
Representative DNA sequence 
>AFM221 (AFM221Q) /CSM/AF/AFM2-A/AFM221Q.Seq.d/
ACACAACAATTTATAAGCTCACCACCACTAAATAATTCAACTGATGAATTTTTAGATTAT
TCCGAGCATGAAAGGTCAGAAATGTCGGTTAGCAATAACCTATGGTACAAAGAAGCAATT
TTTTATGAAGTTTATGTTAGGGCATTTTGTGATATTGAAGGAACTGGTAATGGTGGTATT
TCAGGTATTACAAATAAATTGGATTATTTGCATACACTAGGTGTTGATTGTATTTGGTTA
TTACCAATTTACCCTTCACCTTTAAAAGATGATGGATATGATATTTCAGATTATTGTGAT
ATTCACCCAGATTATGGTACATTGAATGATTTTAAAATTTTAGTTAAAGCAGTTCATGAA
AGAAATATGAAGATCATTGCTGATTTCATTCCAAATCATTGTTCAGATAAACATAAATGG
TTTCAATCTGCAAGATTATCAAGAGATTCACCATATAGAGATTATTTCGTTTGGTCTGAT
TCCCCACAAAAATATAAAGATGCAAGAATCATTTTTTTAGATGTTGAACAATCAAATTGG
ACATGGGATGAAGCAGCAGGACAATATTATTGGCATAGATTTTATAAAGAACAACCAGAT
CTTAATTTTGATAAXXXXXXXXXXCTCATGCTACTCCAGTTTTACCATCATTATCAACTC
ATCAATCTTCAATTTGTGGTGGTGGTGGATCACTTCATCGTAGAACTGCTAGTAATAGTG
GTGATGGTATTGCAAGTGGTATTGGTTCAAGAATTGGCGGTACAAATACTAGTGGTGGTG
GTGGTGGTGGTAATAATGATGGAATTTCAAATATTACAAGTAATTTTGGTAATACTTTAA
CAGTTGATGGTGCAGGTGGTAATTTATCAGGTTCAAAAGTTACNCCAATTCCAACTANAA
AAGTTCATACTCATTATCAACAATCAAATATAAATAGACAACAACATNTTCAACCTTTAA
CAACCAAAGAAGAATATGAAGCATCAAAAGATACTAAACAACCATTTGTTTCATTTTTAA
ATGTTGAAACTGCTTCCTATGATGAACAATCACATTATTTAATAGATATTTTAACAGATT
ATCAAGTGATGGTAGATCAAAAAGGTTATGTTACAATGGATTTAGAACCATACCAATTCT
TTTGGTTCTCAATGGGTGAAATTACAACTTTAATGGGTGATGGTTCAAAATCACGTGATC
TTAGAAGAAAATCAATTCAAATTAAAGATCCAATTAATTTACATCATAGAAATTCTCATT
TTGCCGATCCNATTGATGAATAAATAAATAAAATCNTTTCTAATAATATTACTAATTTAA
AAACAAAACAAAAAAAAAATAAAATA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
TQQFISSPPLNNSTDEFLDYSEHERSEMSVSNNLWYKEAIFYEVYVRAFCDIEGTGNGGI
SGITNKLDYLHTLGVDCIWLLPIYPSPLKDDGYDISDYCDIHPDYGTLNDFKILVKAVHE
RNMKIIADFIPNHCSDKHKWFQSARLSRDSPYRDYFVWSDSPQKYKDARIIFLDVEQSNW
TWDEAAGQYYWHRFYKEQPDLNFD---

---HATPVLPSLSTHQSSICGGGGSLHRRTASNSGDGIASGIGSRIGGTNTSGGGGGGNN
DGISNITSNFGNTLTVDGAGGNLSGSKVTPIPTXKVHTHYQQSNINRQQHXQPLTTKEEY
EASKDTKQPFVSFLNVETASYDEQSHYLIDILTDYQVMVDQKGYVTMDLEPYQFFWFSMG
EITTLMGDGSKSRDLRRKSIQIKDPINLHHRNSHFADPIDE*inkixsnnitnlktkqkk
nki
Translated Amino Acid sequence (All Frames) 
Frame A:
TQQFISSPPLNNSTDEFLDYSEHERSEMSVSNNLWYKEAIFYEVYVRAFCDIEGTGNGGI
SGITNKLDYLHTLGVDCIWLLPIYPSPLKDDGYDISDYCDIHPDYGTLNDFKILVKAVHE
RNMKIIADFIPNHCSDKHKWFQSARLSRDSPYRDYFVWSDSPQKYKDARIIFLDVEQSNW
TWDEAAGQYYWHRFYKEQPDLNFD---

---lmllqfyhhyqlinlqfvvvvdhfivellvivvmvlqvvlvqelavqilvvvvvvvi
mmefqilqvilvil*qlmvqvviyqvqklxqfqlxkfiliinnqi*idnnxfnl*qpkkn
mkhqkilnnhlfhf*mlkllpmmnnhii**if*qiik*w*ikkvmlqwi*nhtnsfgsqw
vklql*wvmvqnhvileenqfklkiqliyiieililpixlmnk*iksfliilli*kqnkk
kik

Frame B:
hnnl*ahhh*iiqlmnf*iipsmkgqkcrlaitygtkkqffmkfmlghfvilkelvmvvf
qvlqinwiicih*vlivfgyyqftlhl*kmmdmifqiiviftqimvh*milkf*lkqfmk
ei*rsllisfqiivqiningfnlqdyqeihhieiisfgliphknikmqesff*mlnnqig
hgmkqqdniigidfiknnqilili---

---scyssftiiinssifnlwwwwitss*nc***w*wyckwywfknwryky*wwwwww**
*wnfkyyk*fw*yfns*wcrw*firfksyxnsnxkssyslstikyk*tttxstfnnqrri
*sikry*tticfifkc*ncfl**titlfnryfnrlssdgrskrlcyngfrtipillvlng
*nynfng*wfkit*s*kkinsn*rsn*fts*kfsfcrsx**ink*nxf**yy*fknktkk
k*n

Frame C:
ttiykltttk*fn**ifrlfra*kvrnvg*q*pmvqrsnfl*slc*gil*y*rnw*wwyf
ryyk*iglfaytrc*lylvitnlpftfkr*wi*yfrll*ysprlwyie*f*nfs*sss*k
kyedhc*fhskslfr*t*mvsickiikrfti*rlfrlv*fptki*rcknhffrc*tikld
mg*ssrtilla*il*rttrs*f**---

---HATPVLPSLSTHQSSICGGGGSLHRRTASNSGDGIASGIGSRIGGTNTSGGGGGGNN
DGISNITSNFGNTLTVDGAGGNLSGSKVTPIPTXKVHTHYQQSNINRQQHXQPLTTKEEY
EASKDTKQPFVSFLNVETASYDEQSHYLIDILTDYQVMVDQKGYVTMDLEPYQFFWFSMG
EITTLMGDGSKSRDLRRKSIQIKDPINLHHRNSHFADPIDE*inkixsnnitnlktkqkk
nki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM221 (AFM221Q) /CSM/AF/AFM2-A/AFM221Q.Seq.d/                       1427   0.0
VHJ358 (VHJ358Q) /CSM/VH/VHJ3-C/VHJ358Q.Seq.d/                       1055   0.0
SLA289 (SLA289Q) /CSM/SL/SLA2-D/SLA289Q.Seq.d/                        926   0.0
AFN120 (AFN120Q) /CSM/AF/AFN1-A/AFN120Q.Seq.d/                        920   0.0
CHH251 (CHH251Q) /CSM/CH/CHH2-C/CHH251Q.Seq.d/                        912   0.0
CFC289 (CFC289Q) /CSM/CF/CFC2-D/CFC289Q.Seq.d/                        912   0.0
AFL570 (AFL570Q) /CSM/AF/AFL5-C/AFL570Q.Seq.d/                        912   0.0
SFK748 (SFK748Q) /CSM/SF/SFK7-B/SFK748Q.Seq.d/                        906   0.0
SSL244 (SSL244Q) /CSM/SS/SSL2-B/SSL244Q.Seq.d/                        787   0.0
SHB186 (SHB186Q) /CSM/SH/SHB1-D/SHB186Q.Seq.d/                        763   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346807) Dictyostelium discoideum cDNA clone:dda25i06, 3' ...  1015   0.0    2
(BJ329477) Dictyostelium discoideum cDNA clone:dda25i06, 5' ...  1013   0.0    2
(C25555) Dictyostelium discoideum slug cDNA, clone SLA289.        999   0.0    2
(BJ379952) Dictyostelium discoideum cDNA clone:ddc36e14, 3' ...   985   0.0    2
(BJ442325) Dictyostelium discoideum cDNA clone:ddv49d15, 3' ...   922   0.0    3
(BJ373991) Dictyostelium discoideum cDNA clone:ddc5a24, 3' e...   912   0.0    3
(BJ346670) Dictyostelium discoideum cDNA clone:dda24k17, 3' ...   912   0.0    2
(BJ423532) Dictyostelium discoideum cDNA clone:ddv49d15, 5' ...  1013   0.0    2
(BJ401201) Dictyostelium discoideum cDNA clone:dds22p11, 3' ...   979   0.0    2
(BJ403973) Dictyostelium discoideum cDNA clone:dds26k21, 3' ...   710   0.0    3
dna update 2009. 4.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ407627_1(DQ407627|pid:none) Hartmannella vermiformis trehalose...   270   8e-71
CP001337_2024(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   231   3e-59
CP000481_675(CP000481|pid:none) Acidothermus cellulolyticus 11B ...   231   3e-59
CP000909_1132(CP000909|pid:none) Chloroflexus aurantiacus J-10-f...   228   4e-58
CP000820_1038(CP000820|pid:none) Frankia sp. EAN1pec, complete g...   228   4e-58
AE017261_69(AE017261|pid:none) Picrophilus torridus DSM 9790, co...   224   7e-57
CP000769_2947(CP000769|pid:none) Anaeromyxobacter sp. Fw109-5, c...   224   7e-57
CP000088_582(CP000088|pid:none) Thermobifida fusca YX, complete ...   223   1e-56
CP000113_3585(CP000113|pid:none) Myxococcus xanthus DK 1622, com...   223   2e-56
CP000359_533(CP000359|pid:none) Deinococcus geothermalis DSM 113...   223   2e-56
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM221 is

5' end seq. ID AFM221F
5' end seq. 
>AFM221F.Seq
ACACAACAATTTATAAGCTCACCACCACTAAATAATTCAACTGATGAATTTTTAGATTAT
TCCGAGCATGAAAGGTCAGAAATGTCGGTTAGCAATAACCTATGGTACAAAGAAGCAATT
TTTTATGAAGTTTATGTTAGGGCATTTTGTGATATTGAAGGAACTGGTAATGGTGGTATT
TCAGGTATTACAAATAAATTGGATTATTTGCATACACTAGGTGTTGATTGTATTTGGTTA
TTACCAATTTACCCTTCACCTTTAAAAGATGATGGATATGATATTTCAGATTATTGTGAT
ATTCACCCAGATTATGGTACATTGAATGATTTTAAAATTTTAGTTAAAGCAGTTCATGAA
AGAAATATGAAGATCATTGCTGATTTCATTCCAAATCATTGTTCAGATAAACATAAATGG
TTTCAATCTGCAAGATTATCAAGAGATTCACCATATAGAGATTATTTCGTTTGGTCTGAT
TCCCCACAAAAATATAAAGATGCAAGAATCATTTTTTTAGATGTTGAACAATCAAATTGG
ACATGGGATGAAGCAGCAGGACAATATTATTGGCATAGATTTTATAAAGAACAACCAGAT
CTTAATTTTGATAAXXXXXXXXXX
Length of 5' end seq. 624
3' end seq. ID AFM221Z
3' end seq. 
>AFM221Z.Seq
XXXXXXXXXXCTCATGCTACTCCAGTTTTACCATCATTATCAACTCATCAATCTTCAATT
TGTGGTGGTGGTGGATCACTTCATCGTAGAACTGCTAGTAATAGTGGTGATGGTATTGCA
AGTGGTATTGGTTCAAGAATTGGCGGTACAAATACTAGTGGTGGTGGTGGTGGTGGTAAT
AATGATGGAATTTCAAATATTACAAGTAATTTTGGTAATACTTTAACAGTTGATGGTGCA
GGTGGTAATTTATCAGGTTCAAAAGTTACNCCAATTCCAACTANAAAAGTTCATACTCAT
TATCAACAATCAAATATAAATAGACAACAACATNTTCAACCTTTAACAACCAAAGAAGAA
TATGAAGCATCAAAAGATACTAAACAACCATTTGTTTCATTTTTAAATGTTGAAACTGCT
TCCTATGATGAACAATCACATTATTTAATAGATATTTTAACAGATTATCAAGTGATGGTA
GATCAAAAAGGTTATGTTACAATGGATTTAGAACCATACCAATTCTTTTGGTTCTCAATG
GGTGAAATTACAACTTTAATGGGTGATGGTTCAAAATCACGTGATCTTAGAAGAAAATCA
ATTCAAATTAAAGATCCAATTAATTTACATCATAGAAATTCTCATTTTGCCGATCCNATT
GATGAATAAATAAATAAAATCNTTTCTAATAATATTACTAATTTAAAAACAAAACAAAAA
AAAAATAAAATA
Length of 3' end seq. 732
Connected seq. ID AFM221P
Connected seq. 
>AFM221P.Seq
ACACAACAATTTATAAGCTCACCACCACTAAATAATTCAACTGATGAATTTTTAGATTAT
TCCGAGCATGAAAGGTCAGAAATGTCGGTTAGCAATAACCTATGGTACAAAGAAGCAATT
TTTTATGAAGTTTATGTTAGGGCATTTTGTGATATTGAAGGAACTGGTAATGGTGGTATT
TCAGGTATTACAAATAAATTGGATTATTTGCATACACTAGGTGTTGATTGTATTTGGTTA
TTACCAATTTACCCTTCACCTTTAAAAGATGATGGATATGATATTTCAGATTATTGTGAT
ATTCACCCAGATTATGGTACATTGAATGATTTTAAAATTTTAGTTAAAGCAGTTCATGAA
AGAAATATGAAGATCATTGCTGATTTCATTCCAAATCATTGTTCAGATAAACATAAATGG
TTTCAATCTGCAAGATTATCAAGAGATTCACCATATAGAGATTATTTCGTTTGGTCTGAT
TCCCCACAAAAATATAAAGATGCAAGAATCATTTTTTTAGATGTTGAACAATCAAATTGG
ACATGGGATGAAGCAGCAGGACAATATTATTGGCATAGATTTTATAAAGAACAACCAGAT
CTTAATTTTGATAA----------CTCATGCTACTCCAGTTTTACCATCATTATCAACTC
ATCAATCTTCAATTTGTGGTGGTGGTGGATCACTTCATCGTAGAACTGCTAGTAATAGTG
GTGATGGTATTGCAAGTGGTATTGGTTCAAGAATTGGCGGTACAAATACTAGTGGTGGTG
GTGGTGGTGGTAATAATGATGGAATTTCAAATATTACAAGTAATTTTGGTAATACTTTAA
CAGTTGATGGTGCAGGTGGTAATTTATCAGGTTCAAAAGTTACNCCAATTCCAACTANAA
AAGTTCATACTCATTATCAACAATCAAATATAAATAGACAACAACATNTTCAACCTTTAA
CAACCAAAGAAGAATATGAAGCATCAAAAGATACTAAACAACCATTTGTTTCATTTTTAA
ATGTTGAAACTGCTTCCTATGATGAACAATCACATTATTTAATAGATATTTTAACAGATT
ATCAAGTGATGGTAGATCAAAAAGGTTATGTTACAATGGATTTAGAACCATACCAATTCT
TTTGGTTCTCAATGGGTGAAATTACAACTTTAATGGGTGATGGTTCAAAATCACGTGATC
TTAGAAGAAAATCAATTCAAATTAAAGATCCAATTAATTTACATCATAGAAATTCTCATT
TTGCCGATCCNATTGATGAATAAATAAATAAAATCNTTTCTAATAATATTACTAATTTAA
AAACAAAACAAAAAAAAAATAAAATA
Length of connected seq. 1336
Full length Seq ID -
Full length Seq. -
Length of full length seq. -