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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC230
Library VF
(Link to library)
Clone ID VFC230
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFC230P
(Link to Original site)
Representative DNA sequence 
>VFC230 (VFC230Q) /CSM/VF/VFC2-B/VFC230Q.Seq.d/
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAATAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCAGTTCTCTTAACCGAAGCCCCATTAAATCCAAAAGCTAACAGAGAAAAAATGACC
CAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTA
TCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTXXXXXX
XXXXGTTACTCATTTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAA
ATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGC
CCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTT
CCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCA
CGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGG
TAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAA
AGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACG
TAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAAT
GTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTT
CTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACAAATATTTATGTAAAATATATAA
TAAAATACATTTAATATTTTTAGGTTTTGTTTAGTTGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllxf**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRH
TGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE
HPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDG--
-

---VTHFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNE
RFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRM
NKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRK
CF*tiktsdesashkylckiynkihliflgfv*l
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxf**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRH
TGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE
HPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDG--
-

---VTHFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNE
RFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRM
NKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRK
CF*tiktsdesashkylckiynkihliflgfv*l

Frame B:
llaywxfnkqifkyntiikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdt
lvlwsvwvkktlt*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvllqkn
tqfs*pkph*iqkltekk*pklclkpstpqpctlpfklsypymplvvppvllwiqvm---

---llisppllkeksseiskkn*ptspstlklkckllphhqpsknhtnyqtvksspsvmn
vsvaqkpssnhhs*vwnqlvstkppitps*nvmlisvkiytvmssylvvqlcsqvlliv*
tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgspkknttnqvhqlsten
vskqlklvmkvlltniyvkyiikyi*yf*vlfsc

Frame C:
cwptgvlinkylniiq**ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskth
wcygrygskrllrr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccsrrt
psslnrspiksks*qrkndpnyv*nlqhpshvrchsscliliclwsyhrycygfr*w---

---ysfhhhc*krnrqryqrkislrrprl*s*nanccliisprkiiritrrsshhhr**t
fplprsplptiilrygiswyprnhl*lhhem*c*yp*rfir*crliwwynyvpryc*sye
qrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlqrrirrirsincpqkm
flnn*n***kcfsqifm*ni**ntfnifrfclv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC230 (VFC230Q) /CSM/VF/VFC2-B/VFC230Q.Seq.d/                       2270   0.0
VFL590 (VFL590Q) /CSM/VF/VFL5-D/VFL590Q.Seq.d/                       1241   0.0
VFJ427 (VFJ427Q) /CSM/VF/VFJ4-B/VFJ427Q.Seq.d/                       1241   0.0
VFI465 (VFI465Q) /CSM/VF/VFI4-C/VFI465Q.Seq.d/                       1241   0.0
VFI423 (VFI423Q) /CSM/VF/VFI4-A/VFI423Q.Seq.d/                       1241   0.0
VFG833 (VFG833Q) /CSM/VF/VFG8-B/VFG833Q.Seq.d/                       1241   0.0
VFC745 (VFC745Q) /CSM/VF/VFC7-B/VFC745Q.Seq.d/                       1241   0.0
SFD794 (SFD794Q) /CSM/SF/SFD7-D/SFD794Q.Seq.d/                       1241   0.0
CFC667 (CFC667Q) /CSM/CF/CFC6-C/CFC667Q.Seq.d/                       1241   0.0
AFE153 (AFE153Q) /CSM/AF/AFE1-C/AFE153Q.Seq.d/                       1241   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1239  0.0  5
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1005  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   946  0.0  7
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        928  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   928  0.0  5
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   833  0.0  2
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   801  0.0  14
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     743  0.0  2
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   737  0.0  9
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   682  0.0  5
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK304552_1(AK304552|pid:none) Homo sapiens cDNA FLJ52842 complet...   333   e-173
(P35432) RecName: Full=Actin-1;  &L07773_1(L07773|pid:none)           323   e-169
AF144405_1(AF144405|pid:none) Pleurochrysis carterae clone PAC11...   320   e-169
(Q8BFZ3) RecName: Full=Beta-actin-like protein 2; AltName: Full=...   318   e-169
(Q03341) RecName: Full=Actin-2;  &L07774_1(L07774|pid:none)           312   e-167
(P07828) RecName: Full=Actin-18; AltName: Full=Actin-3-sub 2;         318   e-165
FN357543_13(FN357543|pid:none) Schistosoma mansoni genome sequen...   333   e-163
A03000(A03000)actin 3 - fruit fly (Drosophila melanogaster) (fra...   314   e-163
DQ980417_1(DQ980417|pid:none) Chrysochromulina sp. NIES-1333 act...   311   e-159
DQ980418_1(DQ980418|pid:none) Chrysochromulina sp. NIES-1333 act...   309   e-158
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFC230 is cyt
5' end seq. ID VFC230F
5' end seq. 
>VFC230F.Seq
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAATAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCAGTTCTCTTAACCGAAGCCCCATTAAATCCAAAAGCTAACAGAGAAAAAATGACC
CAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTA
TCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGT------
----
Length of 5' end seq. 534
3' end seq. ID VFC230Z
3' end seq. 
>VFC230Z.Seq
----------GTTACTCATTTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAA
AGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATC
ATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGA
ACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGG
TATCCACGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTT
ATACGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTAT
GAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACC
AGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCA
ACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTCCACAGAAA
ATGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACAAATATTTATGTAAAAT
ATATAATAAAATACATTTAATATTTTTAGGTTTTGTTTAGTTGT
Length of 3' end seq. 634
Connected seq. ID VFC230P
Connected seq. 
>VFC230P.Seq
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAATAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCAGTTCTCTTAACCGAAGCCCCATTAAATCCAAAAGCTAACAGAGAAAAAATGACC
CAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTA
TCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGT------
----GTTACTCATTTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAA
ATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGC
CCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTT
CCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCA
CGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGG
TAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAA
AGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACG
TAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAAT
GTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTT
CTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACAAATATTTATGTAAAATATATAA
TAAAATACATTTAATATTTTTAGGTTTTGTTTAGTTGT
Length of connected seq. 1168
Full length Seq ID -
Full length Seq. -
Length of full length seq. -