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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC228
Library VF
(Link to library)
Clone ID VFC228
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFC228P
(Link to Original site)
Representative DNA sequence 
>VFC228 (VFC228Q) /CSM/VF/VFC2-B/VFC228Q.Seq.d/
ACTGTTGGCCTACTGGTTCAAAGGNAATCCACTCTCCACTTGGTTCTCAGTATTAAGNAG
GTGGTATGCAAATCTTTGTAAAAACCTTGACTGGTAAAACTATCACCTTGGAAGTCGAAG
GTTCTGACAACATTGAAAATGTTAAAGCCAAAATCCAAGNACAAAGNAAGGTATCCCACC
AGATCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGA
CTACAACATTCAAAAAGAATCCACTCTCCATTTAGTTTTAAGATTAAGAGGTGGTATGCA
AATTTTTGTTAAAACTCTCACTGGTAAGACCATCACCTTAGAGGTTGAAGGTAGTGATAA
CATTGAAAATGTCAAAGCCAAAATCCAAGACAAAGAAGGTATCCCACCAGATCAACAAAG
ATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGACTACAACATCCA
AAAAGAATCCACTCTCCATTTAGTTTTAAGATTAAGAGGTGGTATGCAAATCTTTGTTAA
AACTCTCACTGGTAAAACCATCACCTTGGAAGTCGAAGGTAGTGATAACATTGAAAATGT
NAAAGCCAAAATNCAAGACAAAGAAGGGTATTNCACCAGATCAACAACGTCTCATTTTCG
CTGGTAAACANTTAGGAAGAGGGNCGTACTCTNTNTGATTACAACATCCAAAAAGAATNC
ACTCTNCATTTAGTTCTCAGAATAAGAGGNGGCNTGCAAATCNTTGNTAAAACCTTTGNC
TGGTTAGAACATCACATTGGGAAGTCGAAGGTTTTTNNTACCNTTGAAAATNTNAAGCCC
AAATTCNANGNCAAXXXXXXXXXXCTTCCCTTTTAGNTTTTAAAANTAAAAAGGGGGGTA
TGCAAATTTTTTGTTAAAANCTNCCNCGGGTAAAACCCNTCCCCCTTGGNAAGTNGNAAG
GTAGTGAATAACCNTTTGAAAATNGCAAAAGNCNAAAATCCAANNCAAAAGAAGGTNTTC
CCCCANATCAANAAAAGTTTCTTTTCGNTGGNAAACAATTAAAAAANGGCCGNACTCTCT
NTGATTTCAACATCCAAAAANAATCCACTCTCCNTTTAGTTTTCANATTAANAGGNGGTA
TGCAAATNTTTGTAAAAACCNTGNCTGGTAANACCNTCCCNTTGGAANTNGAAGGTTNTG
ATAACNTTGAAAATGTCAAANCCNAAATTCAANACAAANAAGGTATTCCCCCAGATCAAC
AAANATTNATTTTNGCTGGTNAACAATTANAAAANGGTNGGACTCTCTCTGACTACAATA
TTCAAAANGAATCCCCTNTCCNTTTAGTTNTCANATTAAAAGGTGGTATGCAAATNTTTG
TTAAAACNTTAACAGGTAAAACNATCCCCNTGGAAGTCGAAGGTAGTGATAACNTTNAAA
ATGTTAAANCCNAAATCCNANNCAAANAAGGNNTTCCCCCAGATCAACAACGTCTCNTTT
TCGCTGGTAAACNATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllvqrxstlhlvlsikxvvcksl*kp*lvklspwkskvlttlkmlkpkskxkxrypt
rstkinfrw*tirrwsysl*lqhskrihspfsfkikrwyanfc*nshw*dhhlrg*r***
h*kcqsqnprqrryptrstkinfrw*tirrwsysl*lqhpkrihspfsfkikrwyanlc*
nshw*nhhlgsrr***h*kcxsqnxrqrrvxhqinnvsfslvnx*EEGRTLXDYNIQKEX
TLHLVLRIRGGXQIXXKTFXWLEHHIGKSKVFXTXENXKPKFXX---

---SLLXFKXKKGGMQIFC*xlprvkpxplgkxxgse*pfenxkxxksxxkegxppxqxk
flfxgkqlkxgrtlxdfniqkxstlxlvfxlxggmqxfvktxxgxtxplexegxdnxenv
kxxiqxkxgippdqqxxixagxqlxxgxtlsdyniqxespxxlvxxlkggmqxfvktltg
ktipxevegsdnxxnvkxxixxkxgxppdqqrlxfagkx
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvqrxstlhlvlsikxvvcksl*kp*lvklspwkskvlttlkmlkpkskxkxrypt
rstkinfrw*tirrwsysl*lqhskrihspfsfkikrwyanfc*nshw*dhhlrg*r***
h*kcqsqnprqrryptrstkinfrw*tirrwsysl*lqhpkrihspfsfkikrwyanlc*
nshw*nhhlgsrr***h*kcxsqnxrqrrvxhqinnvsfslvnx*EEGRTLXDYNIQKEX
TLHLVLRIRGGXQIXXKTFXWLEHHIGKSKVFXTXENXKPKFXX---

---lpf*xlkxkrgvckffvkxxxg*npsplxsxkvvnnxlkxakxxnpxqkkvfpxixk
sffsxxnn*kxaxlsxistskxnplsx*fsx*xxvckxl*kpxlvxpsxwxxkvxitlkm
sxpkfxtxkvfpqinkxxfxlvnnxkxvglslttifkxnplsx*xsx*kvvckxllkx*q
vkxspwkskvvitxkmlxpksxxxkxfpqinnvsfslvnx

Frame B:
llaywfkgnplstwfsvlxrwyanlcknldw*nyhlgsrrf*qh*kc*sqnpxtkxgipp
dqqrlifagkqledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdn
ienvkakiqdkegippdqqrlifagkqledgrtlsdyniqkestlhlvlrlrggmqifvk
tltgktitlevegsdnienvkakxqdkegyxtrsttshfrw*txrkrxvlxxittskknx
lxi*fse*exackslxkplxg*nitlgsrrfxxplkxxspnsxx---

---fpfxf*x*kggyanfllkxxxgktxppwxvxr**itx*kxqkxkiqxkrrxspxsxk
vsfrwxtikkxpxsl*fqhpkxihspfsfxixrxyanxcknxxw*xxpxgxxrx**x*kc
qxxnsxqxrysprstxixfxwxtixkxxdsl*lqyskxipxpfsxxikrwyanxc*nxnr
*nxpxgsrr***xxkc*xxnpxqxrxsprsttsxfrw*ti

Frame C:
cwptgskxihsplgsqy*xggmqifvktltgktitlevegsdnienvkakiqxqxkvshq
inkd*fslvnn*kmvvlsltttfkknplsi*f*d*evvckfllklslvrpsp*rlkvvit
lkmskpksktkkvshqinkd*fslvnn*kmvvlsltttskknplsi*f*d*evvcksllk
lslvkpspwkskvvitlkmxkpkxktkkgixpdqqrlifagkxlgrgxysx*lqhpkrxh
sxfssqnkrxxanxx*nlxlvrtshwevegfxyx*kxxaqixxq---

---SLLXFKXKKGGMQIFC*xlprvkpxplgkxxgse*pfenxkxxksxxkegxppxqxk
flfxgkqlkxgrtlxdfniqkxstlxlvfxlxggmqxfvktxxgxtxplexegxdnxenv
kxxiqxkxgippdqqxxixagxqlxxgxtlsdyniqxespxxlvxxlkggmqxfvktltg
ktipxevegsdnxxnvkxxixxkxgxppdqqrlxfagkx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC228 (VFC228Q) /CSM/VF/VFC2-B/VFC228Q.Seq.d/                       2375   0.0
CFD218 (CFD218Q) /CSM/CF/CFD2-A/CFD218Q.Seq.d/                       1281   0.0
AFD485 (AFD485Q) /CSM/AF/AFD4-D/AFD485Q.Seq.d/                       1259   0.0
SFJ591 (SFJ591Q) /CSM/SF/SFJ5-D/SFJ591Q.Seq.d/                       1146   0.0
CFJ540 (CFJ540Q) /CSM/CF/CFJ5-B/CFJ540Q.Seq.d/                       1134   0.0
AFE384 (AFE384Q) /CSM/AF/AFE3-D/AFE384Q.Seq.d/                       1126   0.0
SLK626 (SLK626Q) /CSM/SL/SLK6-B/SLK626Q.Seq.d/                       1015   0.0
CFK385 (CFK385Q) /CSM/CF/CFK3-D/CFK385Q.Seq.d/                        900   0.0
AFG257 (AFG257Q) /CSM/AF/AFG2-C/AFG257Q.Seq.d/                        898   0.0
AFE236 (AFE236Q) /CSM/AF/AFE2-B/AFE236Q.Seq.d/                        896   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   900  0.0  10
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   622  0.0  6
M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.   559  0.0  8
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   630  0.0  3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.   551  0.0  8
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.   551  0.0  8
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   543  0.0  4
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   543  0.0  4
Z54197|Z54197.1 C.albicans UBI4 gene encoding polyubiquitin.     252  e-154  7
Y17558|Y17558.1 Candida albicans UB14 gene.                      252  e-132  7
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   290   e-145
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   290   e-145
AM494963_268(AM494963|pid:none) Leishmania braziliensis chromoso...   283   e-142
X98063_1(X98063|pid:none) P.sylvestris mRNA for polyubiquitin.        286   e-142
AM494972_376(AM494972|pid:none) Leishmania braziliensis chromoso...   283   e-142
AC149642_19(AC149642|pid:none) Medicago truncatula chromosome 2 ...   287   e-141
AF502576_1(AF502576|pid:none) Musa acuminata ubiquitin (UBQ2) ge...   286   e-141
S34285(S34285) polyubiquitin - tomato  &X73156_1(X73156|pid:none)     286   e-141
S43306(S43306)polyubiquitin 6 - Geodia cydonium                       278   e-136
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   290   e-127
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFC228 is nuc
5' end seq. ID VFC228F
5' end seq. 
>VFC228F.Seq
ACTGTTGGCCTACTGGTTCAAAGGNAATCCACTCTCCACTTGGTTCTCAGTATTAAGNAG
GTGGTATGCAAATCTTTGTAAAAACCTTGACTGGTAAAACTATCACCTTGGAAGTCGAAG
GTTCTGACAACATTGAAAATGTTAAAGCCAAAATCCAAGNACAAAGNAAGGTATCCCACC
AGATCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGA
CTACAACATTCAAAAAGAATCCACTCTCCATTTAGTTTTAAGATTAAGAGGTGGTATGCA
AATTTTTGTTAAAACTCTCACTGGTAAGACCATCACCTTAGAGGTTGAAGGTAGTGATAA
CATTGAAAATGTCAAAGCCAAAATCCAAGACAAAGAAGGTATCCCACCAGATCAACAAAG
ATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGACTACAACATCCA
AAAAGAATCCACTCTCCATTTAGTTTTAAGATTAAGAGGTGGTATGCAAATCTTTGTTAA
AACTCTCACTGGTAAAACCATCACCTTGGAAGTCGAAGGTAGTGATAACATTGAAAATGT
NAAAGCCAAAATNCAAGACAAAGAAGGGTATTNCACCAGATCAACAACGTCTCATTTTCG
CTGGTAAACANTTAGGAAGAGGGNCGTACTCTNTNTGATTACAACATCCAAAAAGAATNC
ACTCTNCATTTAGTTCTCAGAATAAGAGGNGGCNTGCAAATCNTTGNTAAAACCTTTGNC
TGGTTAGAACATCACATTGGGAAGTCGAAGGTTTTTNNTACCNTTGAAAATNTNAAGCCC
AAATTCNANGNCAA----------
Length of 5' end seq. 854
3' end seq. ID VFC228Z
3' end seq. 
>VFC228Z.Seq
----------CTTCCCTTTTAGNTTTTAAAANTAAAAAGGGGGGTATGCAAATTTTTTGT
TAAAANCTNCCNCGGGTAAAACCCNTCCCCCTTGGNAAGTNGNAAGGTAGTGAATAACCN
TTTGAAAATNGCAAAAGNCNAAAATCCAANNCAAAAGAAGGTNTTCCCCCANATCAANAA
AAGTTTCTTTTCGNTGGNAAACAATTAAAAAANGGCCGNACTCTCTNTGATTTCAACATC
CAAAAANAATCCACTCTCCNTTTAGTTTTCANATTAANAGGNGGTATGCAAATNTTTGTA
AAAACCNTGNCTGGTAANACCNTCCCNTTGGAANTNGAAGGTTNTGATAACNTTGAAAAT
GTCAAANCCNAAATTCAANACAAANAAGGTATTCCCCCAGATCAACAAANATTNATTTTN
GCTGGTNAACAATTANAAAANGGTNGGACTCTCTCTGACTACAATATTCAAAANGAATCC
CCTNTCCNTTTAGTTNTCANATTAAAAGGTGGTATGCAAATNTTTGTTAAAACNTTAACA
GGTAAAACNATCCCCNTGGAAGTCGAAGGTAGTGATAACNTTNAAAATGTTAAANCCNAA
ATCCNANNCAAANAAGGNNTTCCCCCAGATCAACAACGTCTCNTTTTCGCTGGTAAACNA
TT
Length of 3' end seq. 652
Connected seq. ID VFC228P
Connected seq. 
>VFC228P.Seq
ACTGTTGGCCTACTGGTTCAAAGGNAATCCACTCTCCACTTGGTTCTCAGTATTAAGNAG
GTGGTATGCAAATCTTTGTAAAAACCTTGACTGGTAAAACTATCACCTTGGAAGTCGAAG
GTTCTGACAACATTGAAAATGTTAAAGCCAAAATCCAAGNACAAAGNAAGGTATCCCACC
AGATCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGA
CTACAACATTCAAAAAGAATCCACTCTCCATTTAGTTTTAAGATTAAGAGGTGGTATGCA
AATTTTTGTTAAAACTCTCACTGGTAAGACCATCACCTTAGAGGTTGAAGGTAGTGATAA
CATTGAAAATGTCAAAGCCAAAATCCAAGACAAAGAAGGTATCCCACCAGATCAACAAAG
ATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGACTACAACATCCA
AAAAGAATCCACTCTCCATTTAGTTTTAAGATTAAGAGGTGGTATGCAAATCTTTGTTAA
AACTCTCACTGGTAAAACCATCACCTTGGAAGTCGAAGGTAGTGATAACATTGAAAATGT
NAAAGCCAAAATNCAAGACAAAGAAGGGTATTNCACCAGATCAACAACGTCTCATTTTCG
CTGGTAAACANTTAGGAAGAGGGNCGTACTCTNTNTGATTACAACATCCAAAAAGAATNC
ACTCTNCATTTAGTTCTCAGAATAAGAGGNGGCNTGCAAATCNTTGNTAAAACCTTTGNC
TGGTTAGAACATCACATTGGGAAGTCGAAGGTTTTTNNTACCNTTGAAAATNTNAAGCCC
AAATTCNANGNCAA----------CTTCCCTTTTAGNTTTTAAAANTAAAAAGGGGGGTA
TGCAAATTTTTTGTTAAAANCTNCCNCGGGTAAAACCCNTCCCCCTTGGNAAGTNGNAAG
GTAGTGAATAACCNTTTGAAAATNGCAAAAGNCNAAAATCCAANNCAAAAGAAGGTNTTC
CCCCANATCAANAAAAGTTTCTTTTCGNTGGNAAACAATTAAAAAANGGCCGNACTCTCT
NTGATTTCAACATCCAAAAANAATCCACTCTCCNTTTAGTTTTCANATTAANAGGNGGTA
TGCAAATNTTTGTAAAAACCNTGNCTGGTAANACCNTCCCNTTGGAANTNGAAGGTTNTG
ATAACNTTGAAAATGTCAAANCCNAAATTCAANACAAANAAGGTATTCCCCCAGATCAAC
AAANATTNATTTTNGCTGGTNAACAATTANAAAANGGTNGGACTCTCTCTGACTACAATA
TTCAAAANGAATCCCCTNTCCNTTTAGTTNTCANATTAAAAGGTGGTATGCAAATNTTTG
TTAAAACNTTAACAGGTAAAACNATCCCCNTGGAAGTCGAAGGTAGTGATAACNTTNAAA
ATGTTAAANCCNAAATCCNANNCAAANAAGGNNTTCCCCCAGATCAACAACGTCTCNTTT
TCGCTGGTAAACNATT
Length of connected seq. 1506
Full length Seq ID -
Full length Seq. -
Length of full length seq. -