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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC227
Library VF
(Link to library)
Clone ID VFC227
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16444-1
Original site URL
Representative seq. ID VFC227Z
(Link to Original site)
Representative DNA sequence 
>VFC227 (VFC227Q) /CSM/VF/VFC2-B/VFC227Q.Seq.d/
XXXXXXXXXXGTCAGTTGATGGNCAAAACCATTCATACNGAAATATTCATCATTAAGATC
AGTCGTAGTTGCTGATAAGTCTGAAAAAGTTAAATTACCAATTAATGAACCAGCCAATGG
TATTAGAAAGAGTCAAATTCAAGAATACGTAGATTTCTACAATGGTGCTGGTGTTCAACA
TATCGCCTTAAAGACTGATAACATCATTGATGCTATCTCAAAATTAAGATCTCGTGGTGT
CTCTTTCCTCACTGTTCCAAAAACATACTATACATCACTCAGAGAGAAATTACAACACTC
TTCATTAGAAATTAAAGAAGATTTGGACACTTTAGAGAAATTACACATTTTAATCGATTA
TGATGACAAAGGTTATCTTTTACAAATCTTTACAAATAATGTTGAAGATAAACCAACTGT
TTTCTTTGAAATTATCCAAAGAAACAACCATGATGGTTTCGGTGCTGGTAACTTTAAATC
CCTNCTTTGAAGCAATCGAAAGACAACAAGAAACTCGTGGAAACTTATAGGTGTGTCTCA
ATTACAAATGTTNCTTTATTATTAACTAAATAACATTACAATATATCAAAATAAATAATA
AATAAATANGCTATTTAAAAAGTANTTTTTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---s*WXKPFIXKYSSLRSVVVADKSEKVKLPINEPANGIRKSQIQEYVDFYNGAGVQHI
ALKTDNIIDAISKLRSRGVSFLTVPKTYYTSLREKLQHSSLEIKEDLDTLEKLHILIDYD
DKGYLLQIFTNNVEDKPTVFFEIIQRNNHDGFGAGNFKSLL*snrkttrnswkligvsql
qmxlyy*lnnitiyqnk**inxlfkkxfl
Translated Amino Acid sequence (All Frames) 
Frame A:
---svdgqnhsyxnihh*dqs*llislkklnyqlmnqpmvlervkfknt*istmvlvfni
sp*rlitslmlsqn*dlvvslsslfqkhtihhsernyntlh*klkkiwtl*rnytf*sim
mtkvifykslqimlkinqlfslklskettmmvsvlvtlnpxfeaierqqetrgnl*vcln
ykcxfiin*itlqyikinnk*ixylksxf*


Frame B:
---qlmxktihteifiikisrsc**v*ks*itn**tsqwy*kesnsrirrflqwcwcsty
rlkd**hh*cylkikiswclfphcsknilyitqreittlfirn*rrfghfreithfnrl*
*qrlsftnlyk*c*r*tncfl*nypkkqp*wfrcw*l*ipxlkqskdnkklvetyrcvsi
tnvxllltk*hynisk*iinkxai*kvxf


Frame C:
---s*WXKPFIXKYSSLRSVVVADKSEKVKLPINEPANGIRKSQIQEYVDFYNGAGVQHI
ALKTDNIIDAISKLRSRGVSFLTVPKTYYTSLREKLQHSSLEIKEDLDTLEKLHILIDYD
DKGYLLQIFTNNVEDKPTVFFEIIQRNNHDGFGAGNFKSLL*snrkttrnswkligvsql
qmxlyy*lnnitiyqnk**inxlfkkxfl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC227 (VFC227Q) /CSM/VF/VFC2-B/VFC227Q.Seq.d/                       1078   0.0
CFD404 (CFD404Q) /CSM/CF/CFD4-A/CFD404Q.Seq.d/                       1037   0.0
CFE311 (CFE311Q) /CSM/CF/CFE3-A/CFE311Q.Seq.d/                       1033   0.0
CFD328 (CFD328Q) /CSM/CF/CFD3-B/CFD328Q.Seq.d/                       1031   0.0
VSH226 (VSH226Q) /CSM/VS/VSH2-B/VSH226Q.Seq.d/                       1027   0.0
VSD101 (VSD101Q) /CSM/VS/VSD1-A/VSD101Q.Seq.d/                       1027   0.0
VFO548 (VFO548Q) /CSM/VF/VFO5-B/VFO548Q.Seq.d/                       1027   0.0
VFN489 (VFN489Q) /CSM/VF/VFN4-D/VFN489Q.Seq.d/                       1027   0.0
VFN484 (VFN484Q) /CSM/VF/VFN4-D/VFN484Q.Seq.d/                       1027   0.0
VFN348 (VFN348Q) /CSM/VF/VFN3-B/VFN348Q.Seq.d/                       1027   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116978|AC116978.1 Dictyostelium discoideum chromosome 2 map 4846697-4874480 strain AX4, *** SEQUENCING IN PROGRESS ***.   876  0.0  5
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.   876  0.0  5
BI322343|BI322343.1 kx19h02.y3 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    42  1e-10  4
BM395514|BM395514.1 50072-2-9-E12.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    64  2e-06  1
BI502176|BI502176.1 kt86c08.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    36  2e-05  4
M59429|M59429.1 T. thermophila F-antigen (tfa) gene, complete cds.    46  0.004  2
AC118979|AC118979.7 Rattus norvegicus clone CH230-313J16, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    50  0.033  1
AX283719|AX283719.1 Sequence 13 from Patent WO0179500.            48  0.13  1
AF448502|AF448502.1 Streptococcus thermophilus strain MTC310 exopolysaccharide synthesis gene cluster, partial sequence.    48  0.13  1
AF448249|AF448249.1 Streptococcus thermophilus strain MR-1C exopolysaccharide synthesis gene cluster, complete sequence; insertion sequences IS1193-1, IS1193-2, IS1193-3, IS1193-4, IS1194, IS981C1, IS981C2, IS981SB, ISS1SA, ISS1SB, ISS1SC, partial sequence; and adjacent rpi to orfhppm pseudogenes, partial sequence.    48  0.13  1
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NV5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   293   6e-81
AM114773_1(AM114773|pid:none) Platynereis dumerilii partial mRNA...   215   7e-55
AE014296_3563(AE014296|pid:none) Drosophila melanogaster chromos...   209   4e-53
(P32754) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   207   2e-52
S32821(S32821;S35890;S35889)4-hydroxyphenylpyruvate dioxygenase ...   205   8e-52
(Q02110) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   205   8e-52
(Q5EA20) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   205   8e-52
BC077167_1(BC077167|pid:none) Danio rerio 4-hydroxyphenylpyruvat...   203   9e-52
BC046075_1(BC046075|pid:none) Danio rerio zgc:56326, mRNA (cDNA ...   203   4e-51
(Q6TGZ5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   203   4e-51
protein update 2009. 6.17
PSORT 

 psg: 0.72  gvh: 0.39  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 32.0 %: cytoplasmic
	 28.0 %: nuclear

>> prediction for VFC227 is mit
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VFC227Z
3' end seq. 
>VFC227Z.Seq
----------GTCAGTTGATGGNCAAAACCATTCATACNGAAATATTCATCATTAAGATC
AGTCGTAGTTGCTGATAAGTCTGAAAAAGTTAAATTACCAATTAATGAACCAGCCAATGG
TATTAGAAAGAGTCAAATTCAAGAATACGTAGATTTCTACAATGGTGCTGGTGTTCAACA
TATCGCCTTAAAGACTGATAACATCATTGATGCTATCTCAAAATTAAGATCTCGTGGTGT
CTCTTTCCTCACTGTTCCAAAAACATACTATACATCACTCAGAGAGAAATTACAACACTC
TTCATTAGAAATTAAAGAAGATTTGGACACTTTAGAGAAATTACACATTTTAATCGATTA
TGATGACAAAGGTTATCTTTTACAAATCTTTACAAATAATGTTGAAGATAAACCAACTGT
TTTCTTTGAAATTATCCAAAGAAACAACCATGATGGTTTCGGTGCTGGTAACTTTAAATC
CCTNCTTTGAAGCAATCGAAAGACAACAAGAAACTCGTGGAAACTTATAGGTGTGTCTCA
ATTACAAATGTTNCTTTATTATTAACTAAATAACATTACAATATATCAAAATAAATAATA
AATAAATANGCTATTTAAAAAGTANTTTTTAA
Length of 3' end seq. 622
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -