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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC224
Library VF
(Link to library)
Clone ID VFC224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFC224P
(Link to Original site)
Representative DNA sequence 
>VFC224 (VFC224Q) /CSM/VF/VFC2-A/VFC224Q.Seq.d/
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCXXXXXXXXXXTGAAATGCAAACTGC
TGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCAT
CGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGA
ATCAGCTGGTATCCACGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCG
TAAAGATTTATACGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGC
TGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCAT
TGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTC
AACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGT
CCACAGAAAATGCTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAA
ATATATTTAAATGTATAATAATAATAAAATATAATTATTTTAAGG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllxf**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRH
TGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE
HPVLLTEAPLN---

---EMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSI
MKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIG
GSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnkyi*mynnnki*l
f*
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxf**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRH
TGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE
HPVLLTEAPLN---

---*nanccliisprkiiritrrsshhhr**tfplprsplptiilrygiswyprnhl*lh
hem*c*yp*rfir*crliwwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldw
wiylsftlnfptnvdlqrri*rirsincpqkmllnn*n***kcfshkqiylnv****nii
ilr

Frame B:
llaywxfnkqifkynttikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdt
lvlwsvwvkktlt*vmkpnqrevs*psntqlntvllptgmiwkksgiilstmnsvllqkn
tqfs*lkph*i---

---EMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSI
MKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIG
GSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnkyi*mynnnki*l
f*

Frame C:
cwptgvlinkylniiqq*ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskth
wcygrygskrllrr**spikerylnpqipn*trycyqlg*ygknlasyflq*tpccsrrt
pssln*spiks---

---kckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnqlvstkppitps
*nvmlisvkiytvmssylvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglv
dls*lhsqlsnkcgspkknmtnqvhqlstenaskqlklvmkvlltqtnifkciiiikyny
fk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC224 (VFC224Q) /CSM/VF/VFC2-A/VFC224Q.Seq.d/                       1713   0.0
VFO858 (VFO858Q) /CSM/VF/VFO8-C/VFO858Q.Seq.d/                        967   0.0
VFN701 (VFN701Q) /CSM/VF/VFN7-A/VFN701Q.Seq.d/                        967   0.0
VFM863 (VFM863Q) /CSM/VF/VFM8-C/VFM863Q.Seq.d/                        967   0.0
VFM725 (VFM725Q) /CSM/VF/VFM7-B/VFM725Q.Seq.d/                        967   0.0
VFM720 (VFM720Q) /CSM/VF/VFM7-A/VFM720Q.Seq.d/                        967   0.0
VFM705 (VFM705Q) /CSM/VF/VFM7-A/VFM705Q.Seq.d/                        967   0.0
VFL589 (VFL589Q) /CSM/VF/VFL5-D/VFL589Q.Seq.d/                        967   0.0
VFK573 (VFK573Q) /CSM/VF/VFK5-D/VFK573Q.Seq.d/                        967   0.0
VFJ832 (VFJ832Q) /CSM/VF/VFJ8-B/VFJ832Q.Seq.d/                        967   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   967  0.0  3
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      844  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   833  0.0  5
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        791  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   791  0.0  4
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   712  0.0  9
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     622  0.0  2
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   617  0.0  4
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   575  0.0  5
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   496  0.0  2
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AL160004_3(AL160004|pid:none) Human DNA sequence from clone RP5-...   284   e-134
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   304   3e-81
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   304   3e-81
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        304   3e-81
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   301   2e-80
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   301   2e-80
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   300   6e-80
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   298   1e-79
AY615868_1(AY615868|pid:none) Amastigomonas marina actin gene, p...   298   2e-79
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          297   4e-79
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFC224 is nuc
5' end seq. ID VFC224F
5' end seq. 
>VFC224F.Seq
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCAGTTCTCTTAACTGAAGCCCCATTAAATCC----------
Length of 5' end seq. 395
3' end seq. ID VFC224Z
3' end seq. 
>VFC224Z.Seq
----------TGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAAT
TACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCT
TCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTATAACTCCA
TCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCTGGTG
GTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCC
CATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTG
GTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAAT
ATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGCTTCTAAACAATTAAAACTAGTG
ATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATATAAT
TATTTTAAGG
Length of 3' end seq. 540
Connected seq. ID VFC224P
Connected seq. 
>VFC224P.Seq
ACTGTTGGCCTACTGGNGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATG
GACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGT
TTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACAC
ACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCA
AAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGAT
GATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAA
CACCCAGTTCTCTTAACTGAAGCCCCATTAAATCC----------TGAAATGCAAACTGC
TGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCAT
CGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGA
ATCAGCTGGTATCCACGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCG
TAAAGATTTATACGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGC
TGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCAT
TGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTC
AACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGT
CCACAGAAAATGCTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAA
ATATATTTAAATGTATAATAATAATAAAATATAATTATTTTAAGG
Length of connected seq. 935
Full length Seq ID -
Full length Seq. -
Length of full length seq. -