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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM220
Library AF
(Link to library)
Clone ID AFM220
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM220P
(Link to Original site)
Representative DNA sequence 
>AFM220 (AFM220Q) /CSM/AF/AFM2-A/AFM220Q.Seq.d/
ATTATTTTTTATTTTATAATCACTGTATAAACAAAAATTTATATATATATATATAGAATT
TAAATAAAGATATGTTCAAACAAACATTTAAAGTAAATATTATACCGACCCAACCATTTG
AAGGTCAAAAACCAGGTACATCTGGTTTAAGAAAAAAAGTAACTGAAGTAATGAAGACAA
ATTACCTTGGTAACTTTGTTCAATCAATTTTCAATGCTTTACCAGAAGATAAATTAAAAG
GATCAACTATTGTTGTTGGTGGTGATGGTCGTTACTACAATAATGATGCTATTCAATTAA
TTTTTCAAATTGCAGCAGCCAATGGAGTTGGAAAGATTTTAGTAGGAAGATATGGTTTAC
TTTCAACACCAGCAATCTCTGCAATCGTTCGTGCTCGTTCAGCATTGGGTGCAATCATTT
TAACAGCATCACATAATCCAGGTGGTCCAAATGGTGATTTTGGTATTAAATATAATATGT
CCAATGGTGGTCCAGCTCCAGAATCAATCACCAATGCAATCTATAAACATACCACATCAA
TCACACAAATTAAGACTACTAGAAATGTAAGTGTCGACAATTTGGGTTTGCTTAAAACTT
ATGAATGGAATGATGGGTGAATTCXXXXXXXXXXAATTCTTTGGTAACNTTATGGATGCT
GGTACCCTCTCAATTTGTGGTGAGGAATCATTTGGTACTGGTTCTGATCATATTCGTGAA
AAAGATGGTATTTGGGCTATCATTTGTTGGTTACAAATTTTAACTCATCATAATCAATCA
ACCAATGATAAGAACTTTGTTTCCATTGAAGAGATTGTCAAACAACATTGGGCAAAATAT
GGTAGAAACTATTATTCTCGTTACGATTATGAAGAAATTGACACTGCTCCAGCCGAAGCA
ATGATGAAACATGTTTCTCAACAAATTGAATCCAAACAATTGATTGGTAAGAAATTCACT
GGTATCTCTGATTCTTTGGAATATGAAATTGCATCTTGTGATGATTTTGAATATAAAGAT
CCAATTGATAGTTCAGTTTCCTCTCATCAAGGTCTTCGTATCATTTTCACTGATGGCTCA
AGAATTATCTATCGTTTAAGTGGTACTGGTTCAACTGGTGCCACAGTTCGTGTTTATTTC
GATAAATATGAAACTCAACCTACTCAATTAAATAATGATGTTCAAACTCATCTTAAATCT
TTAATTCATATTGCACTTGTTGAAATTTCAAAATTAAATCATTATACTGGTAGAAATGAA
CCAAATGTAATTACATAAATAAATAAAATTTTAATAAACAAATAAATAAATAAATAAAAA
G
sequence update 2009. 4. 3
Translated Amino Acid sequence 
iifyfiitv*tkiyiyiyri*ikicsnkhlk*ilyrpnhlkvknqvhlv*ekk*lk**rq
itlvtlfnqfsmlyqkin*kdqllllvvmvvttimmlfn*ffklqqpmelerf**edmvy
fqhqqslqsfvlvqhwvqsf*qhhiiqvvqmvilvlniicpmvvqlqnqspmqsiniphq
shklrllem*VSTIWVCLKLMNGMMGEF---

---FFGNXMDAGTLSICGEESFGTGSDHIREKDGIWAIICWLQILTHHNQSTNDKNFVSI
EEIVKQHWAKYGRNYYSRYDYEEIDTAPAEAMMKHVSQQIESKQLIGKKFTGISDSLEYE
IASCDDFEYKDPIDSSVSSHQGLRIIFTDGSRIIYRLSGTGSTGATVRVYFDKYETQPTQ
LNNDVQTHLKSLIHIALVEISKLNHYTGRNEPNVIT*inkilink*ink*k
Translated Amino Acid sequence (All Frames) 
Frame A:
iifyfiitv*tkiyiyiyri*ikicsnkhlk*ilyrpnhlkvknqvhlv*ekk*lk**rq
itlvtlfnqfsmlyqkin*kdqllllvvmvvttimmlfn*ffklqqpmelerf**edmvy
fqhqqslqsfvlvqhwvqsf*qhhiiqvvqmvilvlniicpmvvqlqnqspmqsiniphq
shklrllem*VSTIWVCLKLMNGMMGEF---

---nslvtlwmlvpsqfvvrnhlvlvliifvkkmvfglsfvgykf*liiinqpmirtlfp
lkrlsnnigqnmvetiilvtimkkltllqpkq**nmflnklnpnn*lvrnslvslilwnm
klhlvmilnikiqlivqfplikvfvsfslmaqelsiv*vvlvqlvpqfvfisinmklnll
n*immfklilnl*filhllkfqn*iiilvemnqm*lhk*ikf**tnk*inkk

Frame B:
lffil*slykqkfiyiyiefk*ryvqtni*skyytdpti*rsktryiwfkkksn*snedk
lpw*lcsinfqcftrr*ikrinyccww*wsllq**cysinfsncssqwswkdfsrkiwft
fntsnlcnrscsfsigcnhfnsit*srwskw*fwy*i*yvqwwsssrinhqcnl*tyhin
htn*dy*kckcrqfgfa*nl*me*wvn---

---ilw*xygcwyplnlw*giiwywf*sys*krwylgyhllvtnfnss*sinq**elcfh
*rdcqttlgkiw*kllfslrl*rn*hcssrsndetcfstn*iqtidw*eihwyl*ffgi*
ncil**f*i*rsn**fsflssrssyhfh*wlknylsfkwywfnwchssclfr*i*nstys
ik**csnss*ifnsyctc*nfkikslyw*k*tkcnyink*nfnkqink*ik

Frame C:
yflfynhcinknlyiyi*nlnkdmfkqtfkvniiptqpfegqkpgtsglrkkvtevmktn
ylgnfvqsifnalpedklkgstivvggdgryynndaiqlifqiaaangvgkilvgrygll
stpaisaivrarsalgaiiltashnpggpngdfgikynmsnggpapesitnaiykhttsi
tqikttrnvsvdnlgllktyewndg*i---

---FFGNXMDAGTLSICGEESFGTGSDHIREKDGIWAIICWLQILTHHNQSTNDKNFVSI
EEIVKQHWAKYGRNYYSRYDYEEIDTAPAEAMMKHVSQQIESKQLIGKKFTGISDSLEYE
IASCDDFEYKDPIDSSVSSHQGLRIIFTDGSRIIYRLSGTGSTGATVRVYFDKYETQPTQ
LNNDVQTHLKSLIHIALVEISKLNHYTGRNEPNVIT*inkilink*ink*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM220 (AFM220Q) /CSM/AF/AFM2-A/AFM220Q.Seq.d/                       2268   0.0
VHK768 (VHK768Q) /CSM/VH/VHK7-C/VHK768Q.Seq.d/                       1221   0.0
VHD683 (VHD683Q) /CSM/VH/VHD6-D/VHD683Q.Seq.d/                       1221   0.0
AFO149 (AFO149Q) /CSM/AF/AFO1-C/AFO149Q.Seq.d/                       1221   0.0
AFI424 (AFI424Q) /CSM/AF/AFI4-A/AFI424Q.Seq.d/                       1221   0.0
AFF233 (AFF233Q) /CSM/AF/AFF2-B/AFF233Q.Seq.d/                       1215   0.0
VHL451 (VHL451Q) /CSM/VH/VHL4-C/VHL451Q.Seq.d/                       1213   0.0
AFO365 (AFO365Q) /CSM/AF/AFO3-C/AFO365Q.Seq.d/                       1213   0.0
CHB413 (CHB413Q) /CSM/CH/CHB4-A/CHB413Q.Seq.d/                       1205   0.0
AFM194 (AFM194Q) /CSM/AF/AFM1-D/AFM194Q.Seq.d/                       1205   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U61984) Dictyostelium discoideum phosphoglucomutase A (pgmA...  1239   0.0    1
(BJ443329) Dictyostelium discoideum cDNA clone:ddv52h17, 3' ...  1239   0.0    1
(BJ438893) Dictyostelium discoideum cDNA clone:ddv38e22, 3' ...  1239   0.0    1
(BJ346798) Dictyostelium discoideum cDNA clone:dda25g06, 3' ...  1239   0.0    1
(BJ343799) Dictyostelium discoideum cDNA clone:dda17p06, 3' ...  1239   0.0    1
(BJ345910) Dictyostelium discoideum cDNA clone:dda29a13, 3' ...  1233   0.0    1
(BJ339993) Dictyostelium discoideum cDNA clone:dda11a10, 3' ...  1227   0.0    1
(BJ443626) Dictyostelium discoideum cDNA clone:ddv53f14, 3' ...  1219   0.0    1
(BJ345919) Dictyostelium discoideum cDNA clone:dda29b17, 3' ...  1219   0.0    1
(BJ377574) Dictyostelium discoideum cDNA clone:ddc24j04, 3' ...  1205   0.0    1
dna update 2009. 4.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q23919) RecName: Full=Phosphoglucomutase-1;          Short=PGM ...   436   e-121
AE017356_43(AE017356|pid:none) Cryptococcus neoformans var. neof...   247   6e-64
BT043631_1(BT043631|pid:none) Salmo salar clone HM5_0587 phospho...   246   2e-63
AY059926_1(AY059926|pid:none) Arabidopsis thaliana phosphoglucom...   242   2e-62
(Q9SGC1) RecName: Full=Probable phosphoglucomutase, cytoplasmic ...   242   2e-62
(Q9M4G4) RecName: Full=Phosphoglucomutase, cytoplasmic;         ...   237   8e-61
AK064893_1(AK064893|pid:none) Oryza sativa Japonica Group cDNA c...   237   8e-61
(P93262) RecName: Full=Phosphoglucomutase, cytoplasmic;         ...   236   1e-60
AX030362_1(AX030362|pid:none) Sequence 3 from Patent EP1001029.       235   2e-60
AK293298_1(AK293298|pid:none) Homo sapiens cDNA FLJ50439 complet...   235   3e-60
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM220 is

5' end seq. ID AFM220F
5' end seq. 
>AFM220F.Seq
ATTATTTTTTATTTTATAATCACTGTATAAACAAAAATTTATATATATATATATAGAATT
TAAATAAAGATATGTTCAAACAAACATTTAAAGTAAATATTATACCGACCCAACCATTTG
AAGGTCAAAAACCAGGTACATCTGGTTTAAGAAAAAAAGTAACTGAAGTAATGAAGACAA
ATTACCTTGGTAACTTTGTTCAATCAATTTTCAATGCTTTACCAGAAGATAAATTAAAAG
GATCAACTATTGTTGTTGGTGGTGATGGTCGTTACTACAATAATGATGCTATTCAATTAA
TTTTTCAAATTGCAGCAGCCAATGGAGTTGGAAAGATTTTAGTAGGAAGATATGGTTTAC
TTTCAACACCAGCAATCTCTGCAATCGTTCGTGCTCGTTCAGCATTGGGTGCAATCATTT
TAACAGCATCACATAATCCAGGTGGTCCAAATGGTGATTTTGGTATTAAATATAATATGT
CCAATGGTGGTCCAGCTCCAGAATCAATCACCAATGCAATCTATAAACATACCACATCAA
TCACACAAATTAAGACTACTAGAAATGTAAGTGTCGACAATTTGGGTTTGCTTAAAACTT
ATGAATGGAATGATGGGTGAATTCXXXXXXXXXX
Length of 5' end seq. 634
3' end seq. ID AFM220Z
3' end seq. 
>AFM220Z.Seq
XXXXXXXXXXAATTCTTTGGTAACNTTATGGATGCTGGTACCCTCTCAATTTGTGGTGAG
GAATCATTTGGTACTGGTTCTGATCATATTCGTGAAAAAGATGGTATTTGGGCTATCATT
TGTTGGTTACAAATTTTAACTCATCATAATCAATCAACCAATGATAAGAACTTTGTTTCC
ATTGAAGAGATTGTCAAACAACATTGGGCAAAATATGGTAGAAACTATTATTCTCGTTAC
GATTATGAAGAAATTGACACTGCTCCAGCCGAAGCAATGATGAAACATGTTTCTCAACAA
ATTGAATCCAAACAATTGATTGGTAAGAAATTCACTGGTATCTCTGATTCTTTGGAATAT
GAAATTGCATCTTGTGATGATTTTGAATATAAAGATCCAATTGATAGTTCAGTTTCCTCT
CATCAAGGTCTTCGTATCATTTTCACTGATGGCTCAAGAATTATCTATCGTTTAAGTGGT
ACTGGTTCAACTGGTGCCACAGTTCGTGTTTATTTCGATAAATATGAAACTCAACCTACT
CAATTAAATAATGATGTTCAAACTCATCTTAAATCTTTAATTCATATTGCACTTGTTGAA
ATTTCAAAATTAAATCATTATACTGGTAGAAATGAACCAAATGTAATTACATAAATAAAT
AAAATTTTAATAAACAAATAAATAAATAAATAAAAAG
Length of 3' end seq. 697
Connected seq. ID AFM220P
Connected seq. 
>AFM220P.Seq
ATTATTTTTTATTTTATAATCACTGTATAAACAAAAATTTATATATATATATATAGAATT
TAAATAAAGATATGTTCAAACAAACATTTAAAGTAAATATTATACCGACCCAACCATTTG
AAGGTCAAAAACCAGGTACATCTGGTTTAAGAAAAAAAGTAACTGAAGTAATGAAGACAA
ATTACCTTGGTAACTTTGTTCAATCAATTTTCAATGCTTTACCAGAAGATAAATTAAAAG
GATCAACTATTGTTGTTGGTGGTGATGGTCGTTACTACAATAATGATGCTATTCAATTAA
TTTTTCAAATTGCAGCAGCCAATGGAGTTGGAAAGATTTTAGTAGGAAGATATGGTTTAC
TTTCAACACCAGCAATCTCTGCAATCGTTCGTGCTCGTTCAGCATTGGGTGCAATCATTT
TAACAGCATCACATAATCCAGGTGGTCCAAATGGTGATTTTGGTATTAAATATAATATGT
CCAATGGTGGTCCAGCTCCAGAATCAATCACCAATGCAATCTATAAACATACCACATCAA
TCACACAAATTAAGACTACTAGAAATGTAAGTGTCGACAATTTGGGTTTGCTTAAAACTT
ATGAATGGAATGATGGGTGAATTC----------AATTCTTTGGTAACNTTATGGATGCT
GGTACCCTCTCAATTTGTGGTGAGGAATCATTTGGTACTGGTTCTGATCATATTCGTGAA
AAAGATGGTATTTGGGCTATCATTTGTTGGTTACAAATTTTAACTCATCATAATCAATCA
ACCAATGATAAGAACTTTGTTTCCATTGAAGAGATTGTCAAACAACATTGGGCAAAATAT
GGTAGAAACTATTATTCTCGTTACGATTATGAAGAAATTGACACTGCTCCAGCCGAAGCA
ATGATGAAACATGTTTCTCAACAAATTGAATCCAAACAATTGATTGGTAAGAAATTCACT
GGTATCTCTGATTCTTTGGAATATGAAATTGCATCTTGTGATGATTTTGAATATAAAGAT
CCAATTGATAGTTCAGTTTCCTCTCATCAAGGTCTTCGTATCATTTTCACTGATGGCTCA
AGAATTATCTATCGTTTAAGTGGTACTGGTTCAACTGGTGCCACAGTTCGTGTTTATTTC
GATAAATATGAAACTCAACCTACTCAATTAAATAATGATGTTCAAACTCATCTTAAATCT
TTAATTCATATTGCACTTGTTGAAATTTCAAAATTAAATCATTATACTGGTAGAAATGAA
CCAAATGTAATTACATAAATAAATAAAATTTTAATAAACAAATAAATAAATAAATAAAAA
G
Length of connected seq. 1311
Full length Seq ID -
Full length Seq. -
Length of full length seq. -