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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC218
Library VF
(Link to library)
Clone ID VFC218
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12091-1
Original site URL
Representative seq. ID VFC218P
(Link to Original site)
Representative DNA sequence 
>VFC218 (VFC218Q) /CSM/VF/VFC2-A/VFC218Q.Seq.d/
ACTGTTGGCCTACTGGNAATTTAAAAAACAATTATAAAAACAACAAATACAAAAATGATT
AAGAATAGAAAATTAGATATTACTTCAACTAATGTTGCTGGTATTGGAACAGATTTAGAT
AAAAAATGTAGATTGGATGCAGCTTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCA
CCAGGTCTCAAGATTTGGAGAATTGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCA
TATGGTAAATTCTATGATGGTGATAGTTATATCATTTTACATACTTTTAAAGAAGGTAAT
TCACTTAAACATGATATTCATTTCTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGC
ACTGCTGCTTATAAAACAGTTGAATTAGATGATTTCTTAGGTGGTGCACCAATTCAATAT
CGTCAATGTCAATCATATGAAAGTCCAAGCTTTTTATCATTATTCCCAAAATATTTCATT
TTATCAGXXXXXXXXXXGAAACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAA
ACTGCCCCAACCAAATCTGAAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTT
AAATTCTCTGAAGTTTCACGTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTT
TTCATCATTGATTTAGGTAATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAAT
GAAAAGAAAACAGCATTCAGTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAA
TATACTCCAATAGTTAGAGTTTTAGAAAATGGTACAAATCAATCATTTGAAACTTTATTA
TNCTGCTTAATAATAAAACACAACAACAACAACAACACATTCAAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllxi*KTIIKTTNTKMIKNRKLDITSTNVAGIGTDLDKKCRLDAASTEAQWKGVGQA
PGLKIWRIENFKVVPVPESSYGKFYDGDSYIILHTFKEGNSLKHDIHFFLGTFTTQDEAG
TAAYKTVELDDFLGGAPIQYRQCQSYESPSFLSLFPKYFILS---

---KLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSRGKINKSSLKSEDVFII
DLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRVLENGTNQSFETLLXCL
IIKHNNNNNTFKK
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxi*KTIIKTTNTKMIKNRKLDITSTNVAGIGTDLDKKCRLDAASTEAQWKGVGQA
PGLKIWRIENFKVVPVPESSYGKFYDGDSYIILHTFKEGNSLKHDIHFFLGTFTTQDEAG
TAAYKTVELDDFLGGAPIQYRQCQSYESPSFLSLFPKYFILS---

---etfrwkrcnrc*trncpnqi*ksfiqiirciwft*il*sftw*nq*iliki*rcfhh
*fr**nlyldrfkiitk*kensiqscnsilgkq*tl*iysns*sfrkwyksii*nfixll
nnktqqqqqhiqk

Frame B:
llaywxfkkql*kqqiqk*lrien*illqlmllvleqi*iknvdwmqlqlklngkvlvkh
qvsrfgelkilklsqfqnhhmvnsmmvivisfyillkkvihlnmifisf*vllllkmkla
lllikqln*mis*vvhqfnivnvnhmkvqafyhysqnisfyq---

---KLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSRGKINKSSLKSEDVFII
DLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRVLENGTNQSFETLLXCL
IIKHNNNNNTFKK

Frame C:
cwptgnlknnyknnkyknd*e*kiryyfn*ccwywnrfr*km*igcsfn*ssmercwsst
rsqdlen*kf*scpssriiiw*il*w**lyhftyf*rr*ft*t*ysflfryfyysr*swh
ccl*ns*ir*flrwctnsissmsii*ksklfiiipkifhfi---

---nf*vekvqsllntklpqpnlkkfytnyqmhlvhlnslkfhvvksinph*nlkmfssl
i*vmksilgsvqnhhqmkrkqhsvmqlntw*tinvvnilq*lef*kmvqinhlklyyxa*
**ntttttthsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO524 (VFO524Q) /CSM/VF/VFO5-A/VFO524Q.Seq.d/                        856   0.0
VFO376 (VFO376Q) /CSM/VF/VFO3-D/VFO376Q.Seq.d/                        856   0.0
VFO316 (VFO316Q) /CSM/VF/VFO3-A/VFO316Q.Seq.d/                        856   0.0
VFN887 (VFN887Q) /CSM/VF/VFN8-D/VFN887Q.Seq.d/                        856   0.0
VFN784 (VFN784Q) /CSM/VF/VFN7-D/VFN784Q.Seq.d/                        856   0.0
VFN652 (VFN652Q) /CSM/VF/VFN6-C/VFN652Q.Seq.d/                        856   0.0
VFN571 (VFN571Q) /CSM/VF/VFN5-C/VFN571Q.Seq.d/                        856   0.0
VFM108 (VFM108Q) /CSM/VF/VFM1-A/VFM108Q.Seq.d/                        856   0.0
VFL329 (VFL329Q) /CSM/VF/VFL3-B/VFL329Q.Seq.d/                        856   0.0
VFL167 (VFL167Q) /CSM/VF/VFL1-C/VFL167Q.Seq.d/                        856   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

J03515|J03515.1 Slime mold (D.discoideum) severin mRNA, complete cds.   856  0.0  3
U66525|U66525.1 Dictyostelium discoideum ORFveg114 mRNA, complete cds.    72  1e-09  2
BP190606|BP190606.1 Dugesia japonica cDNA, clone: 04979_HH, expressed in the head.    56  8e-04  1
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          36  0.058  12
AC137821|AC137821.13 Medicago truncatula clone mth2-15o11, WORKING DRAFT SEQUENCE, 2 ordered pieces.    42  0.11  3
AP002028|AP002028.1 Homo sapiens genomic DNA, chromosome 4q22-q24, clone:2043B18.    38  0.13  7
AC104159|AC104159.2 Pan troglodytes clone RP43-82O21, WORKING DRAFT SEQUENCE, 1 ordered pieces.    48  0.19  1
AL031643|AL031643.1 Human DNA sequence from clone 769D20 on chromosome Xp21.1-21.3 Contains EST, STS, GSS.    36  0.19  4
AP004171|AP004171.1 Mycoplasma penetrans genomic DNA, complete sequence, section 2/5.    36  0.30  8
AC002528|AC002528.1 Human BAC clone GS1-542D18 from 7q31-q32, complete sequence.    48  0.59  2
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U70047_1(U70047|pid:none) Physarum polycephalum actin-binding pr...   190   5e-47
AM920435_1012(AM920435|pid:none) Penicillium chrysogenum Wiscons...   187   5e-46
AJ621806_1(AJ621806|pid:none) Suberites ficus mRNA for gelsolin ...   178   2e-43
AM270354_42(AM270354|pid:none) Aspergillus niger contig An16c002...   175   2e-42
S51363(S51363;S49320) actin modulator protein EWAM - earthworm (...   159   1e-37
EU334660_1(EU334660|pid:none) Strongylocentrotus purpuratus vill...   156   8e-37
BC134994_1(BC134994|pid:none) Danio rerio gelsolin a, mRNA (cDNA...   151   3e-35
BC115120_1(BC115120|pid:none) Danio rerio gelsolin a, mRNA (cDNA...   150   6e-35
EF103444_1(EF103444|pid:none) Haliotis discus discus gelsolin mR...   148   3e-34
AY809768_1(AY809768|pid:none) Schistosoma japonicum SJCHGC06031 ...   146   1e-33
protein update 2009. 6.17
PSORT 

 psg: 0.40  gvh: 0.37  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for VFC218 is cyt
5' end seq. ID VFC218F
5' end seq. 
>VFC218F.Seq
ACTGTTGGCCTACTGGNAATTTAAAAAACAATTATAAAAACAACAAATACAAAAATGATT
AAGAATAGAAAATTAGATATTACTTCAACTAATGTTGCTGGTATTGGAACAGATTTAGAT
AAAAAATGTAGATTGGATGCAGCTTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCA
CCAGGTCTCAAGATTTGGAGAATTGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCA
TATGGTAAATTCTATGATGGTGATAGTTATATCATTTTACATACTTTTAAAGAAGGTAAT
TCACTTAAACATGATATTCATTTCTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGC
ACTGCTGCTTATAAAACAGTTGAATTAGATGATTTCTTAGGTGGTGCACCAATTCAATAT
CGTCAATGTCAATCATATGAAAGTCCAAGCTTTTTATCATTATTCCCAAAATATTTCATT
TTATCAG----------
Length of 5' end seq. 487
3' end seq. ID VFC218Z
3' end seq. 
>VFC218Z.Seq
----------GAAACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCC
CAACCAAATCTGAAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTTAAATTCT
CTGAAGTTTCACGTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCATCA
TTGATTTAGGTAATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAATGAAAAGA
AAACAGCATTCAGTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAATATACTC
CAATAGTTAGAGTTTTAGAAAATGGTACAAATCAATCATTTGAAACTTTATTATNCTGCT
TAATAATAAAACACAACAACAACAACAACACATTCAAAAAA
Length of 3' end seq. 391
Connected seq. ID VFC218P
Connected seq. 
>VFC218P.Seq
ACTGTTGGCCTACTGGNAATTTAAAAAACAATTATAAAAACAACAAATACAAAAATGATT
AAGAATAGAAAATTAGATATTACTTCAACTAATGTTGCTGGTATTGGAACAGATTTAGAT
AAAAAATGTAGATTGGATGCAGCTTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCA
CCAGGTCTCAAGATTTGGAGAATTGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCA
TATGGTAAATTCTATGATGGTGATAGTTATATCATTTTACATACTTTTAAAGAAGGTAAT
TCACTTAAACATGATATTCATTTCTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGC
ACTGCTGCTTATAAAACAGTTGAATTAGATGATTTCTTAGGTGGTGCACCAATTCAATAT
CGTCAATGTCAATCATATGAAAGTCCAAGCTTTTTATCATTATTCCCAAAATATTTCATT
TTATCAG----------GAAACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAA
ACTGCCCCAACCAAATCTGAAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTT
AAATTCTCTGAAGTTTCACGTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTT
TTCATCATTGATTTAGGTAATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAAT
GAAAAGAAAACAGCATTCAGTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAA
TATACTCCAATAGTTAGAGTTTTAGAAAATGGTACAAATCAATCATTTGAAACTTTATTA
TNCTGCTTAATAATAAAACACAACAACAACAACAACACATTCAAAAAA
Length of connected seq. 878
Full length Seq ID -
Full length Seq. -
Length of full length seq. -