>VFC217 (VFC217Q) /CSM/VF/VFC2-A/VFC217Q.Seq.d/
ACTGTTGGCCTACTGGNGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATCTT
ATTTATTCACTAGTGAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTAT
CAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAA
CTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTG
ACTATCAAAAAGTTGTTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAG
GTTTTGATTACAAAACTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTG
CTCXXXXXXXXXXACTTAGACGATAACACCATCTACCATTTGAATCCATCAGGTCGTTTC
GTCATTGGTGGCCCAATGGGTGATTCTGGTTTAACCGGTCGTAAGATCATCATTGATTCA
TATGGTGGTTGGGGTGCTCACGGTGGTGGTGCTTTCTCTGGTAAAGATGCCACTAAAGTC
GATCGTTCTGGTGCTTACGCTGCTAGATGGATCGCTAAATCTTTAGTTGCCGCTGGTCTT
GCCGATCGTTGTTTAGTTCAAGTTTCATACTCTATTGGTGTTGCCAAGCCACTCAGTGTC
TTTGTTGATACCTATGGTACCGGTAAAAAGACTGATGCTGAAATCCTTGCCATCATCGAT
AATAACTTTGATTTACGTCCAGGTTGTTTAATGCGTGACCTCCAATTAACTCGTCCAATT
TTCCAAAAAACTGCCNCTGGTGGTCACTTGGTAGAAATGATCCAGACTTTACCTGGGAAA
CCGTCAAAGAA

cwptgvi*VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACET
ATKTGMVMILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIA
---

---LDDNTIYHLNPSGRFVIGGPMGDSGLTGRKIIIDSYGGWGAHGGGAFSGKDATKVDR
SGAYAARWIAKSLVAAGLADRCLVQVSYSIGVAKPLSVFVDTYGTGKKTDAEILAIIDNN
FDLRPGCLMRDLQLTRPIFQKTAXGGHLVEMIQTLPGKPSK

Frame A:
tvgllxlfk*yftqyikchliyslvnpspkviqiksvikyqmlfsmlv*lkihyqkllvk
lqpklvwl*f*vklppkptltikklfvkllkrlvmmthqkvlitklamf*lqlnnnhqil
l---

---t*titpsti*ihqvvsslvaqwvilv*pvvrsslihmvvgvltvvvlslvkmplksi
vlvltlldgslnl*lplvlpivv*fkfhtllvlpshsvsllipmvpvkrlmlkslpssii
tliyvqvv*cvtsn*lvqfskklplvvtw*k*srlylgnrqr

Frame B:
llaywxylsnishni*nvilfih**irhrrssr*nl*ssirccsrclfssrsiikscl*n
cnqnwygydfr*nyhqsqr*lskscs*sc*kdwl**likrf*lqnlqcfscn*ttitryc
---

---lrr*hhlpfesirsfrhwwpng*fwfnrs*dhh*fiwwlgcsrwwcflw*rch*srs
fwclrc*mdr*ifscrwscrslfsssfilywccqatqclc*ylwyr*kd*c*npchhr**
l*ftsrlfna*ppinssnfpkncxwwslgrndpdftwetvke

Frame C:
cwptgvi*VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACET
ATKTGMVMILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIA
---

---LDDNTIYHLNPSGRFVIGGPMGDSGLTGRKIIIDSYGGWGAHGGGAFSGKDATKVDR
SGAYAARWIAKSLVAAGLADRCLVQVSYSIGVAKPLSVFVDTYGTGKKTDAEILAIIDNN
FDLRPGCLMRDLQLTRPIFQKTAXGGHLVEMIQTLPGKPSK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC217 (VFC217Q) /CSM/VF/VFC2-A/VFC217Q.Seq.d/                       1616   0.0
VFN128 (VFN128Q) /CSM/VF/VFN1-B/VFN128Q.Seq.d/                        928   0.0
VFM766 (VFM766Q) /CSM/VF/VFM7-C/VFM766Q.Seq.d/                        928   0.0
VFM340 (VFM340Q) /CSM/VF/VFM3-B/VFM340Q.Seq.d/                        928   0.0
VFL319 (VFL319Q) /CSM/VF/VFL3-A/VFL319Q.Seq.d/                        928   0.0
VFL243 (VFL243Q) /CSM/VF/VFL2-B/VFL243Q.Seq.d/                        928   0.0
VFJ166 (VFJ166Q) /CSM/VF/VFJ1-C/VFJ166Q.Seq.d/                        928   0.0
VFI607 (VFI607Q) /CSM/VF/VFI6-A/VFI607Q.Seq.d/                        928   0.0
VFI164 (VFI164Q) /CSM/VF/VFI1-C/VFI164Q.Seq.d/                        928   0.0
VFI116 (VFI116Q) /CSM/VF/VFI1-A/VFI116Q.Seq.d/                        928   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ390497|AJ390497.1 Candida albicans partial mRNA for S-adenosylmethionine synthetase 2  (sam2 gene).   107  3e-48  4
AX595308|AX595308.1 Sequence 962 from Patent EP1258494.          109  1e-43  4
M23368|M23368.1 Saccharomyces cerevisiae S-adenosylmethionine synthetase (SAM2) gene, complete cds.   109  6e-43  4
U33057|U33057.1 Saccharomyces cerevisiae chromosome IV cosmids 8166, 9787, 9717, and lambda 3073.   109  2e-38  4
AJ001705|AJ001705.1 Schizosaccharomyces pombe sam1 gene.          90  2e-30  6
AL424144|AL424144.1 T7 end of clone AZ0AA012F03 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    78  7e-26  4
T37377|T37377.1 EST102487 S. cerevisiae strain X2180-1A Saccharomyces cerevisiae cDNA 5' end, mRNA sequence.    70  5e-24  3
AL023780|AL023780.1 S.pombe chromosome II cosmid c14F5.           90  1e-23  6
CF094963|CF094963.1 QHN19K01.yg.ab1 QH_N sunflower H.argophyllus (drought stress) Helianthus argophyllus cDNA clone QHN19K01, mRNA sequence.   100  2e-22  2
AL440787|AL440787.1 T3 end of clone BD0AA014G05 of library BD0AA from strain CBS 94 of  Candida tropicalis.   107  7e-20  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54F07) RecName: Full=S-adenosylmethionine synthetase;         ...   258   2e-67
AB461925_1(AB461925|pid:none) Ditylum brightwellii MAT mRNA for ...   192   4e-51
FJ404748_1(FJ404748|pid:none) Porphyra yezoensis putative S-aden...   202   1e-50
AY324626_1(AY324626|pid:none) Amoeba proteus S-adenosylmethionin...   200   2e-50
AB461924_1(AB461924|pid:none) Cylindrotheca closterium MAT mRNA ...   196   8e-49
AB461922_1(AB461922|pid:none) Asterionella glacialis MAT mRNA fo...   184   1e-48
AC008146_27(AC008146|pid:none) Trypanosoma brucei chromosome 6 c...   192   2e-47
BC089770_1(BC089770|pid:none) Rattus norvegicus methionine adeno...   191   2e-47
(O60198) RecName: Full=S-adenosylmethionine synthetase;         ...   189   8e-47
AE017341_393(AE017341|pid:none) Cryptococcus neoformans var. neo...   189   8e-47

 psg: 0.88  gvh: 0.51  alm: 0.41  top: 0.37  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 20.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VFC217 is cyt

>VFC217F.Seq
ACTGTTGGCCTACTGGNGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATCTT
ATTTATTCACTAGTGAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTAT
CAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAA
CTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTG
ACTATCAAAAAGTTGTTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAG
GTTTTGATTACAAAACTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTG
CTC----------

>VFC217Z.Seq
----------ACTTAGACGATAACACCATCTACCATTTGAATCCATCAGGTCGTTTCGTC
ATTGGTGGCCCAATGGGTGATTCTGGTTTAACCGGTCGTAAGATCATCATTGATTCATAT
GGTGGTTGGGGTGCTCACGGTGGTGGTGCTTTCTCTGGTAAAGATGCCACTAAAGTCGAT
CGTTCTGGTGCTTACGCTGCTAGATGGATCGCTAAATCTTTAGTTGCCGCTGGTCTTGCC
GATCGTTGTTTAGTTCAAGTTTCATACTCTATTGGTGTTGCCAAGCCACTCAGTGTCTTT
GTTGATACCTATGGTACCGGTAAAAAGACTGATGCTGAAATCCTTGCCATCATCGATAAT
AACTTTGATTTACGTCCAGGTTGTTTAATGCGTGACCTCCAATTAACTCGTCCAATTTTC
CAAAAAACTGCCNCTGGTGGTCACTTGGTAGAAATGATCCAGACTTTACCTGGGAAACCG
TCAAAGAA

>VFC217P.Seq
ACTGTTGGCCTACTGGNGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATCTT
ATTTATTCACTAGTGAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTAT
CAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAA
CTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTG
ACTATCAAAAAGTTGTTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAG
GTTTTGATTACAAAACTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTG
CTC----------ACTTAGACGATAACACCATCTACCATTTGAATCCATCAGGTCGTTTC
GTCATTGGTGGCCCAATGGGTGATTCTGGTTTAACCGGTCGTAAGATCATCATTGATTCA
TATGGTGGTTGGGGTGCTCACGGTGGTGGTGCTTTCTCTGGTAAAGATGCCACTAAAGTC
GATCGTTCTGGTGCTTACGCTGCTAGATGGATCGCTAAATCTTTAGTTGCCGCTGGTCTT
GCCGATCGTTGTTTAGTTCAAGTTTCATACTCTATTGGTGTTGCCAAGCCACTCAGTGTC
TTTGTTGATACCTATGGTACCGGTAAAAAGACTGATGCTGAAATCCTTGCCATCATCGAT
AATAACTTTGATTTACGTCCAGGTTGTTTAATGCGTGACCTCCAATTAACTCGTCCAATT
TTCCAAAAAACTGCCNCTGGTGGTCACTTGGTAGAAATGATCCAGACTTTACCTGGGAAA
CCGTCAAAGAA