>VFC216 (VFC216Q) /CSM/VF/VFC2-A/VFC216Q.Seq.d/
ACTGTTGGCCTACTGGAATTTATTTATTTATTCAAATATATATTATATTAAATAAAATGT
TTGCCAGATTAGCCAGAGCTAACTACTTAAAGAATTCAGGTAGATACTTCTCTACTGAGC
CAACCTTAAAAGAAAGATTAGTTCAAATCATCCCAGGTGAAATTGAACAAGTTAAACAAT
TAAAAACTGAACATGGTGACAAAATCATTGGTACCTGTACTGTTGCACAAGCTTATGGTG
GTATGAGATCAGTTAAATCATTAGTTACTGAAACATCATCATTAGATCCAGAAGAAGGCA
TTAGATTCCGTGGTTTAACAXXXXXXXXXX

CWPTGIYLFIQIYIILNKMFARLARANYLKNSGRYFSTEPTLKERLVQIIPGEIEQVKQL
KTEHGDKIIGTCTVAQAYGGMRSVKSLVTETSSLDPEEGIRFRGL---

Frame A:
tvgllefiylfkyily*ikclpd*peltt*riqvdtsllsqp*kkd*fkssqvklnklnn
*klnmvtkslvpvllhklmvv*dqlnh*llkhhh*iqkkaldsvv*---


Frame B:
llaywnlfiysniyyik*nvcqisqs*llkefr*illy*anlkrkissnhpr*n*ts*ti
kn*tw*qnhwylycctslwwyeis*iisy*niiirsrrrh*ipwfn---


Frame C:
CWPTGIYLFIQIYIILNKMFARLARANYLKNSGRYFSTEPTLKERLVQIIPGEIEQVKQL
KTEHGDKIIGTCTVAQAYGGMRSVKSLVTETSSLDPEEGIRFRGL---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC216 (VFC216Q) /CSM/VF/VFC2-A/VFC216Q.Seq.d/                        634   0.0
AFI184 (AFI184Q) /CSM/AF/AFI1-D/AFI184Q.Seq.d/                        611   e-174
VFC191 (VFC191Q) /CSM/VF/VFC1-D/VFC191Q.Seq.d/                        605   e-172
VFO812 (VFO812Q) /CSM/VF/VFO8-A/VFO812Q.Seq.d/                        595   e-169
VFO296 (VFO296Q) /CSM/VF/VFO2-D/VFO296Q.Seq.d/                        595   e-169
VFO156 (VFO156Q) /CSM/VF/VFO1-C/VFO156Q.Seq.d/                        595   e-169
VFO142 (VFO142Q) /CSM/VF/VFO1-B/VFO142Q.Seq.d/                        595   e-169
VFN189 (VFN189Q) /CSM/VF/VFN1-D/VFN189Q.Seq.d/                        595   e-169
VFM621 (VFM621Q) /CSM/VF/VFM6-A/VFM621Q.Seq.d/                        595   e-169
VFM570 (VFM570Q) /CSM/VF/VFM5-C/VFM570Q.Seq.d/                        595   e-169

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   208  e-137  4
CD450751|CD450751.1 USDA-FP_102714 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-37_E02 5', mRNA sequence.    46  3e-04  2
CB832670|CB832670.1 USDA-FP_100461 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-07_A07 5', mRNA sequence.    46  6e-04  2
AL929351|AL929351.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 1/4.    42  0.001  3
AC096296|AC096296.7 Rattus norvegicus clone CH230-36L16, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    54  0.001  1
AZ625948|AZ625948.1 1M0465J21R Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC1M0465J21 R, DNA sequence.    44  0.002  2
BY671414|BY671414.1 Mus musculus 14.5 days embryo df/df Rathke's pouches cDNA, RIKEN full-length enriched library, clone:K820019J08, 3' end partial sequence.    42  0.005  2
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          42  0.007  3
AL773598|AL773598.5 Zebrafish DNA sequence from clone CH211-207G4.    46  0.008  7
AC110061|AC110061.1 Homo sapiens chromosome 15 clone CTD-3185H1 map 15, LOW-PASS SEQUENCE SAMPLING.    44  0.009  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553V1) RecName: Full=Citrate synthase, mitochondrial;         ...   174   1e-42
AC116305_8(AC116305|pid:none) Dictyostelium discoideum chromosom...   172   2e-42
FN392319_422(FN392319|pid:none) Pichia pastoris GS115 chromosome...    95   9e-19
FM992695_315(FM992695|pid:none) Candida dubliniensis CD36 chromo...    92   4e-18
(P79024) RecName: Full=Citrate synthase, mitochondrial;         ...    91   1e-17
AY126274_1(AY126274|pid:none) Candida krusei citrate synthase (C...    89   5e-17
AY144810_1(AY144810|pid:none) Saccharomyces bayanus clone Contig...    89   6e-17
(P00890) RecName: Full=Citrate synthase, mitochondrial;         ...    88   1e-16
AF193854_1(AF193854|pid:none) Saccharomyces kluyveri putative ci...    87   1e-16
CU928180_527(CU928180|pid:none) Kluyveromyces thermotolerans str...    87   1e-16

 psg: 0.90  gvh: 0.62  alm: 0.37  top: 0.73  tms: 0.00  mit: 0.36  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: extracellular, including cell wall
	  4.0 %: vacuolar

>> prediction for VFC216 is nuc

>VFC216F.Seq
ACTGTTGGCCTACTGGAATTTATTTATTTATTCAAATATATATTATATTAAATAAAATGT
TTGCCAGATTAGCCAGAGCTAACTACTTAAAGAATTCAGGTAGATACTTCTCTACTGAGC
CAACCTTAAAAGAAAGATTAGTTCAAATCATCCCAGGTGAAATTGAACAAGTTAAACAAT
TAAAAACTGAACATGGTGACAAAATCATTGGTACCTGTACTGTTGCACAAGCTTATGGTG
GTATGAGATCAGTTAAATCATTAGTTACTGAAACATCATCATTAGATCCAGAAGAAGGCA
TTAGATTCCGTGGTTTAACA----------