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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC208
Library VF
(Link to library)
Clone ID VFC208
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16239-1
Original site URL
Representative seq. ID VFC208P
(Link to Original site)
Representative DNA sequence 
>VFC208 (VFC208Q) /CSM/VF/VFC2-A/VFC208Q.Seq.d/
ACTGTTGGCCTACTGGNATAATAAAAGATTGGTAAAAAACCATGTCAAAATTATTAATTT
TATTGTTATTATCATTGGTTGCTTCAATCTTTTCAACTCCATTGGATGATTATGTCAATG
CTCCTGACGATACCTACAAGTGGACATTAAATAATACAATTGAATATGAAACTTTTACAG
GTTACATTTTAGAGTTGACATCACAAACATGGATGGCTGAAAAATCAGATTGGCCAGTTT
GGAAACATTGGGTTTCAATTTGTGTACCAAAAGGTGTTACAACTACAACTACATTTATAT
ATGTTGATGGTGGTTCAAATGATAACTGGAAAGTACCAGGTTCAATGGATCAAACAATTG
AAATTGTTTGTCTTTCATCTGGTTCAGTTAGTGTTGGTTTAACTCAAATTCCAAATCAAC
CAATCATTTTCAATAATGATGGTGTTCAAAGATTTGAAGATGATCTCGTTGCTTATACTT
GGAGACAATTTTTAGGAAATACAAGTGAACCACTTTGGTTAXXXXXXXXXXATCGTACCA
AATGCTGAACACTCTTTAATGGGTCATCAAATTGATATCATTTTATCGATTGTTACATTT
GTTANATTATTAATTACAAATCAACCACGTCCAACTTTTACATGGGATATTACCTACTCT
GAANATTTAAATAGTGGNACAATCGTTTTAACAGTACCANAAGGTGGTATCATTCCATAC
AAAGTTAAAGTTTGGACAGCAGTAACTGAAAGTACAACCANAAGANATTTCCGTATCATC
ACATGTATGGATATCACCAAATGTATTCAATTCATCATTTGGGATCCTTNTGATATTACT
CCAACTTCCACTGGTGNANATTCAATCNCTTTATCAAAACCANATGCTGGTTGGANAGCN
TTCTTCCTTGAAGCTGAATATTTATATGCTAAAAATTCAATCGATGATGAATATACCCNT
AAATTCACCTCTGAAGTTGCCATTGTACCAAATACTTTACCATTCGGTTCATGCCCTGAA
NANAATGCATGTGGTGAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
cwptgiikdw*KTMSKLLILLLLSLVASIFSTPLDDYVNAPDDTYKWTLNNTIEYETFTG
YILELTSQTWMAEKSDWPVWKHWVSICVPKGVTTTTTFIYVDGGSNDNWKVPGSMDQTIE
IVCLSSGSVSVGLTQIPNQPIIFNNDGVQRFEDDLVAYTWRQFLGNTSEPLWL---

---IVPNAEHSLMGHQIDIILSIVTFVXLLITNQPRPTFTWDITYSEXLNSGTIVLTVPX
GGIIPYKVKVWTAVTESTTXRXFRIITCMDITKCIQFIIWDPXDITPTSTGXXSIXLSKP
XAGWXAFFLEAEYLYAKNSIDDEYTXKFTSEVAIVPNTLPFGSCPEXNACGD
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllx**kigkkpcqny*fycyyhwllqsfqlhwmimsmlltiptsgh*iiqlnmkllq
vtf*s*hhkhgwlknqigqfgnigfqfvyqkvlqlqlhlymlmvvqmitgkyqvqwikql
klfvfhlvqlvlv*lkfqinqsfsimmvfkdlkmisllilgdnf*eiqvnhfg---

---IVPNAEHSLMGHQIDIILSIVTFVXLLITNQPRPTFTWDITYSEXLNSGTIVLTVPX
GGIIPYKVKVWTAVTESTTXRXFRIITCMDITKCIQFIIWDPXDITPTSTGXXSIXLSKP
XAGWXAFFLEAEYLYAKNSIDDEYTXKFTSEVAIVPNTLPFGSCPEXNACGD

Frame B:
llaywxnkrlvknhvkiinfiviiigcfnlfnsig*lcqcs*rylqvdik*yn*i*nfyr
lhfrvditnmdg*kirlasletlgfnlctkrcynynyiyic*wwfk**lestrfngsnn*
nclsfiwfs*cwfnsnskstnhfq**wcski*r*srclyletifrkyk*ttlv---

---syqmlntl*wviklisfyrllhllxy*lqinhvqllhgilptlxi*ivxqsf*qyxk
vvsfhtklkfgqq*lkvqpxxisvsshvwispnvfnssfgilxillqlplvxiqslyqnx
mlvgxxsslklniymlkiqsmmnipxnsplklplyqilyhsvhalxxmhvv

Frame C:
cwptgiikdw*KTMSKLLILLLLSLVASIFSTPLDDYVNAPDDTYKWTLNNTIEYETFTG
YILELTSQTWMAEKSDWPVWKHWVSICVPKGVTTTTTFIYVDGGSNDNWKVPGSMDQTIE
IVCLSSGSVSVGLTQIPNQPIIFNNDGVQRFEDDLVAYTWRQFLGNTSEPLWL---

---rtkc*tlfngssn*yhfidcyicxiinyksttsnfymgyyll*xfk*wxnrfnstxr
wyhsiqs*sldssn*kynxkxfpyhhmygyhqmysihhlgsx*yysnfhwxxfnxfiktx
cwlxsxlp*s*ific*kfnr**iyp*ihl*schctkyftirfmp*xxcmw*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC208 (VFC208Q) /CSM/VF/VFC2-A/VFC208Q.Seq.d/                       1897   0.0
VFO540 (VFO540Q) /CSM/VF/VFO5-B/VFO540Q.Seq.d/                        999   0.0
VFN732 (VFN732Q) /CSM/VF/VFN7-B/VFN732Q.Seq.d/                        999   0.0
VFN176 (VFN176Q) /CSM/VF/VFN1-D/VFN176Q.Seq.d/                        999   0.0
VFL666 (VFL666Q) /CSM/VF/VFL6-C/VFL666Q.Seq.d/                        999   0.0
VFL320 (VFL320Q) /CSM/VF/VFL3-A/VFL320Q.Seq.d/                        999   0.0
VFL194 (VFL194Q) /CSM/VF/VFL1-D/VFL194Q.Seq.d/                        999   0.0
VFK341 (VFK341Q) /CSM/VF/VFK3-B/VFK341Q.Seq.d/                        999   0.0
VFI702 (VFI702Q) /CSM/VF/VFI7-A/VFI702Q.Seq.d/                        999   0.0
VFI304 (VFI304Q) /CSM/VF/VFI3-A/VFI304Q.Seq.d/                        999   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116989|AC116989.2 Dictyostelium discoideum chromosome 2 map complement(3527391-3470188) strain AX4, complete sequence.    38  0.022  6
AL731608|AL731608.3 Oryza sativa genomic DNA, chromosome 4, BAC clone: OSJNBa0082E08.    38  0.50  4
Z68213|Z68213.1 Caenorhabditis elegans cosmid C01F6.              44  0.78  2
BX255970|BX255970.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-136G2.    46  0.89  1
AZ202314|AZ202314.1 SP_0056_A2_F09_SP6E Strongylocentrotus purpuratus, purple sea urchin, sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=56 Col=18 Row=K, DNA sequence.    46  0.89  1
BX147732|BX147732.1 Danio rerio genomic clone DKEY-106C4, genomic survey sequence.    46  0.89  1
BD140494|BD140494.1 Elongation factor-2 kinase (EF-2 kinase) and methods of use therefor.    38  0.99  2
AR193041|AR193041.1 Sequence 7 from patent US 6346406.            38  0.99  2
U90946|U90946.1 Dictyostelium discoideum myosin heavy chain kinase B (MHCK-B) mRNA, complete cds.    38  0.99  2
AL929210|AL929210.4 Zebrafish DNA sequence from clone CH211-142E15.    42  1.1  3
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5XM24) RecName: Full=Autocrine proliferation repressor; AltNam...   315   1e-84
AC116987_4(AC116987|pid:none) Dictyostelium discoideum chromosom...   124   5e-27
CP000473_2389(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   101   4e-20
CP000127_1294(CP000127|pid:none) Nitrosococcus oceani ATCC 19707...    59   2e-07
AE015451_4974(AE015451|pid:none) Pseudomonas putida KT2440 compl...    53   2e-05
CP000712_4860(CP000712|pid:none) Pseudomonas putida F1, complete...    52   3e-05
AE003849_1380(AE003849|pid:none) Xylella fastidiosa 9a5c, comple...    51   8e-05
AE008922_2895(AE008922|pid:none) Xanthomonas campestris pv. camp...    49   2e-04
AM920689_1230(AM920689|pid:none) Xanthomonas campestris pv. camp...    49   3e-04
CU694393_139(CU694393|pid:none) Ralstonia solanacearum strain Mo...    49   4e-04
protein update 2009. 6.17
PSORT 

 psg: 0.97  gvh: 0.90  alm: 0.28  top: 0.13  tms: 0.07  mit: 0.42  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: extracellular, including cell wall
	 32.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi

>> prediction for VFC208 is exc
5' end seq. ID VFC208F
5' end seq. 
>VFC208F.Seq
ACTGTTGGCCTACTGGNATAATAAAAGATTGGTAAAAAACCATGTCAAAATTATTAATTT
TATTGTTATTATCATTGGTTGCTTCAATCTTTTCAACTCCATTGGATGATTATGTCAATG
CTCCTGACGATACCTACAAGTGGACATTAAATAATACAATTGAATATGAAACTTTTACAG
GTTACATTTTAGAGTTGACATCACAAACATGGATGGCTGAAAAATCAGATTGGCCAGTTT
GGAAACATTGGGTTTCAATTTGTGTACCAAAAGGTGTTACAACTACAACTACATTTATAT
ATGTTGATGGTGGTTCAAATGATAACTGGAAAGTACCAGGTTCAATGGATCAAACAATTG
AAATTGTTTGTCTTTCATCTGGTTCAGTTAGTGTTGGTTTAACTCAAATTCCAAATCAAC
CAATCATTTTCAATAATGATGGTGTTCAAAGATTTGAAGATGATCTCGTTGCTTATACTT
GGAGACAATTTTTAGGAAATACAAGTGAACCACTTTGGTTA----------
Length of 5' end seq. 521
3' end seq. ID VFC208Z
3' end seq. 
>VFC208Z.Seq
----------ATCGTACCAAATGCTGAACACTCTTTAATGGGTCATCAAATTGATATCAT
TTTATCGATTGTTACATTTGTTANATTATTAATTACAAATCAACCACGTCCAACTTTTAC
ATGGGATATTACCTACTCTGAANATTTAAATAGTGGNACAATCGTTTTAACAGTACCANA
AGGTGGTATCATTCCATACAAAGTTAAAGTTTGGACAGCAGTAACTGAAAGTACAACCAN
AAGANATTTCCGTATCATCACATGTATGGATATCACCAAATGTATTCAATTCATCATTTG
GGATCCTTNTGATATTACTCCAACTTCCACTGGTGNANATTCAATCNCTTTATCAAAACC
ANATGCTGGTTGGANAGCNTTCTTCCTTGAAGCTGAATATTTATATGCTAAAAATTCAAT
CGATGATGAATATACCCNTAAATTCACCTCTGAAGTTGCCATTGTACCAAATACTTTACC
ATTCGGTTCATGCCCTGAANANAATGCATGTGGTGAT
Length of 3' end seq. 507
Connected seq. ID VFC208P
Connected seq. 
>VFC208P.Seq
ACTGTTGGCCTACTGGNATAATAAAAGATTGGTAAAAAACCATGTCAAAATTATTAATTT
TATTGTTATTATCATTGGTTGCTTCAATCTTTTCAACTCCATTGGATGATTATGTCAATG
CTCCTGACGATACCTACAAGTGGACATTAAATAATACAATTGAATATGAAACTTTTACAG
GTTACATTTTAGAGTTGACATCACAAACATGGATGGCTGAAAAATCAGATTGGCCAGTTT
GGAAACATTGGGTTTCAATTTGTGTACCAAAAGGTGTTACAACTACAACTACATTTATAT
ATGTTGATGGTGGTTCAAATGATAACTGGAAAGTACCAGGTTCAATGGATCAAACAATTG
AAATTGTTTGTCTTTCATCTGGTTCAGTTAGTGTTGGTTTAACTCAAATTCCAAATCAAC
CAATCATTTTCAATAATGATGGTGTTCAAAGATTTGAAGATGATCTCGTTGCTTATACTT
GGAGACAATTTTTAGGAAATACAAGTGAACCACTTTGGTTA----------ATCGTACCA
AATGCTGAACACTCTTTAATGGGTCATCAAATTGATATCATTTTATCGATTGTTACATTT
GTTANATTATTAATTACAAATCAACCACGTCCAACTTTTACATGGGATATTACCTACTCT
GAANATTTAAATAGTGGNACAATCGTTTTAACAGTACCANAAGGTGGTATCATTCCATAC
AAAGTTAAAGTTTGGACAGCAGTAACTGAAAGTACAACCANAAGANATTTCCGTATCATC
ACATGTATGGATATCACCAAATGTATTCAATTCATCATTTGGGATCCTTNTGATATTACT
CCAACTTCCACTGGTGNANATTCAATCNCTTTATCAAAACCANATGCTGGTTGGANAGCN
TTCTTCCTTGAAGCTGAATATTTATATGCTAAAAATTCAATCGATGATGAATATACCCNT
AAATTCACCTCTGAAGTTGCCATTGTACCAAATACTTTACCATTCGGTTCATGCCCTGAA
NANAATGCATGTGGTGAT
Length of connected seq. 1028
Full length Seq ID -
Full length Seq. -
Length of full length seq. -