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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC204
Library VF
(Link to library)
Clone ID VFC204
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFC204P
(Link to Original site)
Representative DNA sequence 
>VFC204 (VFC204Q) /CSM/VF/VFC2-A/VFC204Q.Seq.d/
ATGGGTAAAGGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAA
ATCAACCACCACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGA
AAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTAT
GGATAAATTAAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATT
CGAAACCTCCAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAA
GAATATGATTACTGGTACCTCCCAGGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAAC
TGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGC
TTACACTTTAGGTGTCAAACAAATGATCGTTGCTATCAACAAGATGGATGAAAAATCAAC
CAACTACTCACAAGCCCGTTACGACGAAXXXXXXXXXXCGTATTCCATTACAAGATGTCT
ACAAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAAC
CAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAA
TGCATCACGAACAACCCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAA
ACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCAC
CACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCC
ATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTG
AAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCG
CCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGT
GTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGAC
AAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTG
ATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATNTTNCTTATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
WVKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGKQSFKYAWVM
DKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADCAVLVIASPT
GEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDE---

---RIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQPPEAR
PGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDC
HTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPP
LGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innxxyk
Translated Amino Acid sequence (All Frames) 
Frame A:
mgkgensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*kgsf*ng*tilqirlgy
g*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeydywylpgrlcrlshclpn
w*irswyrqkrsns*trspclhfrcqtndrcyqqdg*kinqlltsplrr---

---RIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQPPEAR
PGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDC
HTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPP
LGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innxxyk

Frame B:
WVKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGKQSFKYAWVM
DKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADCAVLVIASPT
GEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDE---

---vfhykmstrsvvsvlfq*vvsklvslnqvwssplpqlvsqlksnqskcitnnpqkpv
qvtm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvksmlvthqs*iv
tlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnpslnthh
*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*ixxli

Frame C:
g*rrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksmkrkllkwvnnpsntpglw
in*rlnvkevspsillygnskppnttspslmpqvteissri*llvpprpivps*slppql
vnsklvspktvklvntlslltl*vsnk*sllstrwmknqptthkpvtt---

---ysitrclqdrwyrycssrscrnwyh*trygrhlcpswsln*sqisrnasrttprsps
r*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnpcwlltslrls
hcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilh*ipti
rsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeink*xxl*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC204 (VFC204Q) /CSM/VF/VFC2-A/VFC204Q.Seq.d/                       2305   0.0
VFN474 (VFN474Q) /CSM/VF/VFN4-D/VFN474Q.Seq.d/                       1322   0.0
VFN174 (VFN174Q) /CSM/VF/VFN1-D/VFN174Q.Seq.d/                       1322   0.0
VFN108 (VFN108Q) /CSM/VF/VFN1-A/VFN108Q.Seq.d/                       1322   0.0
VFM606 (VFM606Q) /CSM/VF/VFM6-A/VFM606Q.Seq.d/                       1322   0.0
VFM496 (VFM496Q) /CSM/VF/VFM4-D/VFM496Q.Seq.d/                       1322   0.0
VFM193 (VFM193Q) /CSM/VF/VFM1-D/VFM193Q.Seq.d/                       1322   0.0
VFM190 (VFM190Q) /CSM/VF/VFM1-D/VFM190Q.Seq.d/                       1322   0.0
VFM163 (VFM163Q) /CSM/VF/VFM1-C/VFM163Q.Seq.d/                       1322   0.0
VFL819 (VFL819Q) /CSM/VF/VFL8-A/VFL819Q.Seq.d/                       1322   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1275  0.0  2
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1245  0.0  2
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.  1047  0.0  3
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   236  e-180  8
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   184  e-179  9
AX109715|AX109715.1 Sequence 448 from Patent WO0123604.          127  e-150  8
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.          127  e-150  8
AF402092|AF402092.1 Pichia anomala strain NRRL Y-366 translation elongation factor 1-alpha gene, partial cds.   131  e-150  8
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.          153  e-148  8
AX109681|AX109681.1 Sequence 414 from Patent WO0123604.          153  e-148  8
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   396   e-109
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   396   e-109
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   396   e-109
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   318   3e-85
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   316   9e-85
AY643816_1(AY643816|pid:none) Lycogala epidendrum elongation fac...   316   1e-84
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   315   2e-84
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   315   3e-84
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   314   4e-84
FN392319_1522(FN392319|pid:none) Pichia pastoris GS115 chromosom...   314   4e-84
protein update 2009. 6.17
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFC204 is cyt
5' end seq. ID VFC204F
5' end seq. 
>VFC204F.Seq
ATGGGTAAAGGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAA
ATCAACCACCACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGA
AAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTAT
GGATAAATTAAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATT
CGAAACCTCCAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAA
GAATATGATTACTGGTACCTCCCAGGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAAC
TGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGC
TTACACTTTAGGTGTCAAACAAATGATCGTTGCTATCAACAAGATGGATGAAAAATCAAC
CAACTACTCACAAGCCCGTTACGACGAA----------
Length of 5' end seq. 508
3' end seq. ID VFC204Z
3' end seq. 
>VFC204Z.Seq
----------CGTATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGTACTGTTCC
AGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGCCCCAGC
TGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAACAACCCCCAGAAGCCCG
TCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGG
TATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCA
AGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTG
TCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTAC
TGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGC
TATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACC
ATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATC
AACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAA
AAAGAAATAAATAAATAATNTTNCTTATAAA
Length of 3' end seq. 681
Connected seq. ID VFC204P
Connected seq. 
>VFC204P.Seq
ATGGGTAAAGGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAA
ATCAACCACCACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGA
AAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTAT
GGATAAATTAAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATT
CGAAACCTCCAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAA
GAATATGATTACTGGTACCTCCCAGGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAAC
TGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGC
TTACACTTTAGGTGTCAAACAAATGATCGTTGCTATCAACAAGATGGATGAAAAATCAAC
CAACTACTCACAAGCCCGTTACGACGAA----------CGTATTCCATTACAAGATGTCT
ACAAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAAC
CAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAA
TGCATCACGAACAACCCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAA
ACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCAC
CACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCC
ATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTG
AAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCG
CCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGT
GTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGAC
AAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTG
ATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATNTTNCTTATAAA
Length of connected seq. 1189
Full length Seq ID -
Full length Seq. -
Length of full length seq. -