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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC194
Library VF
(Link to library)
Clone ID VFC194
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFC194P
(Link to Original site)
Representative DNA sequence 
>VFC194 (VFC194Q) /CSM/VF/VFC1-D/VFC194Q.Seq.d/
CTGTTGGCCTACTGGNATTGTTATAATCAACTTTAAAGGAAAGGAATAAACAACCATGGG
TAAAGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAAC
CACCACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTA
TGAAAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAA
ATTAAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAAC
CTCCAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATAT
GATTACTGGTACCTCCCAAGCCGATXXXXXXXXXXAAGTCAAATCAGTCGAAATGCATCA
CGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTC
AGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGA
AACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGG
TTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGT
CGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGT
CTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGA
ATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGT
CGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAA
AGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
cwptgiviinfkgke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKY
EKEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNM
ITGTSQAD---

---VKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVI
VLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMV
ELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK
*in
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywxcynql*rkginnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv
*kgsf*ng*tilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqey
dywylpsr---

---ksnqskcitnnsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllkl
ssstiqvksmlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplw
sn*phlvqcvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkr
nk*i

Frame B:
cwptgiviinfkgke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKY
EKEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNM
ITGTSQAD---

---sqisrnasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssy
rpqpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*cryg
rinpissnvc*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikke
ink*

Frame C:
vgllxll*stlkernkqpwvkrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksm
krkllkwvnnpsntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*
llvppkp---

---VKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVI
VLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMV
ELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK
*in
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                       1078   0.0
VSD740 (VSD740Q) /CSM/VS/VSD7-B/VSD740Q.Seq.d/                       1078   0.0
VSD380 (VSD380Q) /CSM/VS/VSD3-D/VSD380Q.Seq.d/                       1078   0.0
VSD312 (VSD312Q) /CSM/VS/VSD3-A/VSD312Q.Seq.d/                       1078   0.0
VFN474 (VFN474Q) /CSM/VF/VFN4-D/VFN474Q.Seq.d/                       1078   0.0
VFN472 (VFN472Q) /CSM/VF/VFN4-C/VFN472Q.Seq.d/                       1078   0.0
VFN448 (VFN448Q) /CSM/VF/VFN4-B/VFN448Q.Seq.d/                       1078   0.0
VFN415 (VFN415Q) /CSM/VF/VFN4-A/VFN415Q.Seq.d/                       1078   0.0
VFN376 (VFN376Q) /CSM/VF/VFN3-D/VFN376Q.Seq.d/                       1078   0.0
VFN340 (VFN340Q) /CSM/VF/VFN3-B/VFN340Q.Seq.d/                       1078   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1037  0.0  2
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1007  0.0  2
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   809  0.0  3
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   182  e-144  7
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   254  e-143  6
AX595858|AX595858.1 Sequence 1512 from Patent EP1258494.         103  e-116  7
AX111255|AX111255.1 Sequence 1988 from Patent WO0123604.         103  e-116  7
AX111254|AX111254.1 Sequence 1987 from Patent WO0123604.         103  e-116  7
AX109932|AX109932.1 Sequence 665 from Patent WO0123604.          103  e-116  7
X00779|X00779.1 Yeast gene for elongation factor 1-alpha (EF-1-alpha).   103  e-115  7
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   318   2e-85
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   318   2e-85
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   318   2e-85
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   247   5e-64
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   246   1e-63
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   244   4e-63
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   243   9e-63
FN392319_1522(FN392319|pid:none) Pichia pastoris GS115 chromosom...   242   2e-62
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   241   2e-62
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   241   2e-62
protein update 2009. 6.17
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFC194 is nuc
5' end seq. ID VFC194F
5' end seq. 
>VFC194F.Seq
CTGTTGGCCTACTGGNATTGTTATAATCAACTTTAAAGGAAAGGAATAAACAACCATGGG
TAAAGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAAC
CACCACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTA
TGAAAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAA
ATTAAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAAC
CTCCAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATAT
GATTACTGGTACCTCCCAAGCCGAT----------
Length of 5' end seq. 385
3' end seq. ID VFC194Z
3' end seq. 
>VFC194Z.Seq
----------AAGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCA
GGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATG
GTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTT
ATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCAC
ACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGT
GCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATG
GTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTA
GGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACC
GTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAG
AAATAAATAAATAA
Length of 3' end seq. 544
Connected seq. ID VFC194P
Connected seq. 
>VFC194P.Seq
CTGTTGGCCTACTGGNATTGTTATAATCAACTTTAAAGGAAAGGAATAAACAACCATGGG
TAAAGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAAC
CACCACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTA
TGAAAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAA
ATTAAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAAC
CTCCAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATAT
GATTACTGGTACCTCCCAAGCCGAT----------AAGTCAAATCAGTCGAAATGCATCA
CGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTC
AGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGA
AACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGG
TTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGT
CGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGT
CTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGA
ATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGT
CGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAA
AGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAA
Length of connected seq. 929
Full length Seq ID -
Full length Seq. -
Length of full length seq. -