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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC193
Library VF
(Link to library)
Clone ID VFC193
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFC193P
(Link to Original site)
Representative DNA sequence 
>VFC193 (VFC193Q) /CSM/VF/VFC1-D/VFC193Q.Seq.d/
ACTGTTGGCCTACTGGNATTAATAAAAACTTAAAATAAATTATATATAAAAAAAAATGGA
AGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGCAAAGCCGGTTT
TGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACAC
TGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATCAAA
GAGAGGTATCTTAACTCTTAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGA
TATGGAAAAAATCTGGCATCATACTTTCTACAATGAACXXXXXXXXXXAAGCCCTCTTCC
AACCATCATTNTTAGGTATGGAATCAGCTGGTATCCACGAAACCACATACAACTCCATCA
TGAAATGTGATGTCGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCAGGTGGTA
CAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCAT
CAACAATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTG
GATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCTAAAGAAGAATATG
ACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAAACAAACAAAAAACTA
GTGATGAAAGTGCTTCCTCACAAATATTTATGTAAAATATATATATAATAAATTTTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywx**klkinyi*KKMEGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHT
GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE---

---ALFQPSXLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKE
LTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*
tnkqktsdesassqifm*niyiinf
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxlikt*nklyikkngr*rcssfsyr*rfwyvqsrfcw**cptccfpincwsskth
wcygwygskgficr**ssikerylns*ipn*trycyqlg*ygknlasyflq*---

---kpssnhhx*vwnqlvstkphttps*nvmsisvkiytvtssyqvvqlcsqvlliv*tk
n*ll*phqq*klksllhqnvntlsglvdls*lhsqlsnkcgslkknmtnqvhqlstenvs
kqtnkklvmkvlphkylckiyi**il

Frame B:
llaywx**klkinyi*KKMEGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHT
GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE---

---splptiixrygiswyprnhiqlhhem*cryp*rfir*rrlirwynyvpryc*syeqr
incfspinnen*nhcstrt*ilcldwwiylsftlnfptnvdl*rri*rirsincpqkmfl
nkqtkn***kcfltniyvkyiynkf*

Frame C:
cwptginknlk*iiykkkwkvkmfkl*lsitvlvcakpvllvmmphvlfshqllvvqdtl
vlwlvwvkrihm*vmklnqrevs*llntqlntvllptgmiwkksgiilstmn---

---ALFQPSXLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKE
LTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*
tnkqktsdesassqifm*niyiinf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC193 (VFC193Q) /CSM/VF/VFC1-D/VFC193Q.Seq.d/                       1417   0.0
VSA750 (VSA750Q) /CSM/VS/VSA7-C/VSA750Q.Seq.d/                        831   0.0
VFL183 (VFL183Q) /CSM/VF/VFL1-D/VFL183Q.Seq.d/                        831   0.0
VFH756 (VFH756Q) /CSM/VF/VFH7-C/VFH756Q.Seq.d/                        831   0.0
VFE643 (VFE643Q) /CSM/VF/VFE6-B/VFE643Q.Seq.d/                        831   0.0
SFF549 (SFF549Q) /CSM/SF/SFF5-C/SFF549Q.Seq.d/                        831   0.0
FCL-AC27 (FCL-AC27Q) /CSM/FCL/FCL-AC/FCL-AC27Q.Seq.d/                 831   0.0
FCL-AB04 (FCL-AB04Q) /CSM/FCL/FCL-AB/FCL-AB04Q.Seq.d/                 831   0.0
AFI406 (AFI406Q) /CSM/AF/AFI4-A/AFI406Q.Seq.d/                        831   0.0
VFK285 (VFK285Q) /CSM/VF/VFK2-D/VFK285Q.Seq.d/                        829   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   654  0.0  7
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      630  0.0  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        624  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   624  0.0  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   615  0.0  7
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   553  0.0  10
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   529  0.0  7
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     535  0.0  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   474  0.0  4
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   424  0.0  2
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A61043(A61043)actin CA15 - sea squirt (Styela clava) (fragments)      189   7e-96
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   234   2e-60
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   234   2e-60
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   234   2e-60
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        234   2e-60
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   232   9e-60
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   232   9e-60
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   231   1e-59
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   229   6e-59
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   229   7e-59
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VFC193 is cyt
5' end seq. ID VFC193F
5' end seq. 
>VFC193F.Seq
ACTGTTGGCCTACTGGNATTAATAAAAACTTAAAATAAATTATATATAAAAAAAAATGGA
AGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGCAAAGCCGGTTT
TGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACAC
TGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATCAAA
GAGAGGTATCTTAACTCTTAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGA
TATGGAAAAAATCTGGCATCATACTTTCTACAATGAAC----------
Length of 5' end seq. 338
3' end seq. ID VFC193Z
3' end seq. 
>VFC193Z.Seq
----------AAGCCCTCTTCCAACCATCATTNTTAGGTATGGAATCAGCTGGTATCCAC
GAAACCACATACAACTCCATCATGAAATGTGATGTCGATATCCGTAAAGATTTATACGGT
AACGTCGTCTTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAA
GAATTAACTGCTTTAGCCCCATCAACAATGAAAATTAAAATCATTGCTCCACCAGAACGT
AAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCTAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTC
TAAACAAACAAACAAAAAACTAGTGATGAAAGTGCTTCCTCACAAATATTTATGTAAAAT
ATATATATAATAAATTTTAA
Length of 3' end seq. 430
Connected seq. ID VFC193P
Connected seq. 
>VFC193P.Seq
ACTGTTGGCCTACTGGNATTAATAAAAACTTAAAATAAATTATATATAAAAAAAAATGGA
AGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGCAAAGCCGGTTT
TGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACAC
TGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATCAAA
GAGAGGTATCTTAACTCTTAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGA
TATGGAAAAAATCTGGCATCATACTTTCTACAATGAAC----------AAGCCCTCTTCC
AACCATCATTNTTAGGTATGGAATCAGCTGGTATCCACGAAACCACATACAACTCCATCA
TGAAATGTGATGTCGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCAGGTGGTA
CAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCAT
CAACAATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTG
GATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCTAAAGAAGAATATG
ACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAAACAAACAAAAAACTA
GTGATGAAAGTGCTTCCTCACAAATATTTATGTAAAATATATATATAATAAATTTTAA
Length of connected seq. 768
Full length Seq ID -
Full length Seq. -
Length of full length seq. -