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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC187
Library VF
(Link to library)
Clone ID VFC187
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12289-1
Original site URL
Representative seq. ID VFC187P
(Link to Original site)
Representative DNA sequence 
>VFC187 (VFC187Q) /CSM/VF/VFC1-D/VFC187Q.Seq.d/
ACTGTTGGCCTACTGGNAACCAATATTTTTTTTTTANATAATTATGGATAATTTTGATTT
TGTATCAGGATTTGGNGCCAGTAGNGGAAGNGGAAGTGGATCAGGNGGTATTTCAACTAT
TTCANGTACTGGNGGTACTGGCCAATCAAGNGGTNCAGTAACTAGTNCTACAAGTNCACC
AACTCCAAGTTTTACATCAACAACTTCAACAATTTCATCAACTACAACAATTTNTACTAC
CACAACAGGATCATCANANCATTCAGGATCATCATATTCAGGATCAGTAATTTCAAGTTT
TATCACTTCAATGATTACAAGTGCAAGTGAAAGTTTAAGTGGAAGTGTAAGTGGTAGCAG
CTNTAGNGATAGCAGTAGCTNTAGTGGTAGCAGCTNTAGTGGTAGTAGTTAXXXXXXXXX
XTAAATTATTTAAAAAATAATAATTGTAACTATTTATATTCCTGTCACATTTTTAATGTA
TGGTATTTATGATGACATTTGCAATGTTAGCAGATTCATTATCAAGTAACTTTTACTTAA
AAACTTAGAGATTTTGTTTCAGTCGCAGATGTTGATTTAACATCAACAGTTGGAACATTC
TTTTTATATTGTGGTTTGGCAGATGTTGGAATTATTAATCCAAAAGAAACAAAAACTAAA
ATTATTTTATTATTCTCTTCTAAATGGTGTGCTCACTTTGGTGCTCATTTCTCTTCAAGT
TATGCATGGTTCTTTTTAAGTGATTGTGCTATGCTCTTAATTTCTCATTATGTAACTGTT
TCAAGAGGTTCCAAATCACCATCATCGCAATCATTCTATAATAAGCAACCATTATTATCA
AAATTAAATAATGACACTGTTAACAAATCTACAATCATCATTCCATCTGCATATAAATCA
CCAGTTGTGCAATCTTATTTCATTTCTGTACCACAACAAGTACAACCACAACAATATCGT
TTTGTTCCACAACAACCACAACCAGTTTTTTATGCTAACCAAATTCAACAACAACAACAA
CAAGACAAACCATTAAGTTATATTGTTAAGAAACACTAAGAAATTTAATAAGAAATTTCA
AAAAAAATAATTAAAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLAYWXPIFFFXIIMDNFDFVSGFGASXGXGSGSGGISTISXTGGTGQSXGXVTSXTSXP
TPSFTSTTSTISSTTTIXTTTTGSSXHSGSSYSGSVISSFITSMITSASESLSGSVSGSS
XXDSSSXSGSSXSGSS---

---NYLKNNNCNYLYSCHIFNVWYL**hlqc*qihyqvtft*klrdfvsvadvdltstvg
tfflycgladvgiinpketktkiillfsskwcahfgahfsssyawfflsdcamllishyv
tvsrgskspssqsfynkqpllsklnndtvnkstiiipsaykspvvqsyfisvpqqvqpqq
yrfvpqqpqpvfyanqiqqqqqqdkplsyivkkh*ei**eiskkiiki
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxtnifflxnyg*f*fciriwxq*xkxkwirxyfnyfxywxywpikxxsn*xykxt
nskfyinnfnnfinynnxyyhnriixxfriiifrisnfkfyhfndykck*kfkwkckw*q
l*x*q*l*w*ql*w**l---

---*ii*kiiivtiyipvtflmygiyddicnvsrfiik*lllknleilfqsqmli*hqql
ehsfyivvwqmlelliqkkqklklfyysllngvltlvlislqvmhgsf*vivlcs*flim
*lfqevpnhhhrnhsiisnhyyqn*imtlltnlqssfhlhinhqlcnlisflyhnkynhn
nivlfhnnhnqffmltkfnnnnnktnh*villrntkkfnkkfqkk*lk*

Frame B:
LLAYWXPIFFFXIIMDNFDFVSGFGASXGXGSGSGGISTISXTGGTGQSXGXVTSXTSXP
TPSFTSTTSTISSTTTIXTTTTGSSXHSGSSYSGSVISSFITSMITSASESLSGSVSGSS
XXDSSSXSGSSXSGSS---

---klfkk**l*lfiflshf*cmvfmmtfamladslssnfylkt*rfcfsrrc*fninsw
nilfilwfgrcwny*skrnkn*nyfiilf*mvcslwcsflfklcmvlfk*lcyalnfslc
ncfkrfqitiiaiil**atiiikik**hc*qiynhhsici*itscailfhfctttstttt
isfcstttttsflc*pnstttttrqtiklyc*etlrnlirnfkknn*n

Frame C:
cwptgnqyfffx*lwiililyqdlxpvxexevdqxvfqlfxvlxvlanqxvq*lvlqvhq
lqvlhqqlqqfhqlqqfxlpqqdhxxiqdhhiqdq*fqvlslq*lqvqvkv*vev*vvaa
xxiavaxvvaaxvvvv---

---NYLKNNNCNYLYSCHIFNVWYL**hlqc*qihyqvtft*klrdfvsvadvdltstvg
tfflycgladvgiinpketktkiillfsskwcahfgahfsssyawfflsdcamllishyv
tvsrgskspssqsfynkqpllsklnndtvnkstiiipsaykspvvqsyfisvpqqvqpqq
yrfvpqqpqpvfyanqiqqqqqqdkplsyivkkh*ei**eiskkiiki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC187 (VFC187Q) /CSM/VF/VFC1-D/VFC187Q.Seq.d/                       1683   0.0
SSM589 (SSM589Q) /CSM/SS/SSM5-D/SSM589Q.Seq.d/                        640   0.0
VFH394 (VFH394Q) /CSM/VF/VFH3-D/VFH394Q.Seq.d/                        636   0.0
VFH812 (VFH812Q) /CSM/VF/VFH8-A/VFH812Q.Seq.d/                        626   e-178
VFN457 (VFN457Q) /CSM/VF/VFN4-C/VFN457Q.Seq.d/                        589   e-167
VFL181 (VFL181Q) /CSM/VF/VFL1-D/VFL181Q.Seq.d/                        589   e-167
VFC703 (VFC703Q) /CSM/VF/VFC7-A/VFC703Q.Seq.d/                        589   e-167
VFA485 (VFA485Q) /CSM/VF/VFA4-D/VFA485Q.Seq.d/                        589   e-167
VFD693 (VFD693Q) /CSM/VF/VFD6-D/VFD693Q.Seq.d/                        581   e-165
VSJ225 (VSJ225Q) /CSM/VS/VSJ2-B/VSJ225Q.Seq.d/                        103   6e-21
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    36  0.008  12
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    32  0.012  9
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    40  0.031  11
AX344511|AX344511.1 Sequence 358 from Patent WO0200926.           50  0.060  1
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          40  0.10  10
AC121869|AC121869.2 Mus musculus clone RP24-121C10, complete sequence.    46  0.11  5
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    34  0.11  8
AB065375|AB065375.1 Venerupis philippinarum mitochondrial DNA, complete genome, female type.    40  0.14  5
AC127020|AC127020.12 Medicago truncatula clone mth2-9p1, complete sequence.    38  0.22  6
AY321476|AY321476.1 Tribolium castaneum Achaete-Scute complex gene cluster, partial sequence.    36  0.22  6
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000783_4079(CP000783|pid:none) Enterobacter sakazakii ATCC BAA...    37   0.93
CP000964_225(CP000964|pid:none) Klebsiella pneumoniae 342, compl...    37   1.6
CP000647_3842(CP000647|pid:none) Klebsiella pneumoniae subsp. pn...    37   1.6
(Q8BFU8) RecName: Full=Vesicular glutamate transporter 3;       ...    35   6.0
(Q54LB8) RecName: Full=Putative vacuolar protein sorting-associa...    35   6.1
protein update 2009. 6.17
PSORT 

 psg: 1.00  gvh: 0.52  alm: 0.38  top: 0.33  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFC187 is cyt
5' end seq. ID VFC187F
5' end seq. 
>VFC187F.Seq
ACTGTTGGCCTACTGGNAACCAATATTTTTTTTTTANATAATTATGGATAATTTTGATTT
TGTATCAGGATTTGGNGCCAGTAGNGGAAGNGGAAGTGGATCAGGNGGTATTTCAACTAT
TTCANGTACTGGNGGTACTGGCCAATCAAGNGGTNCAGTAACTAGTNCTACAAGTNCACC
AACTCCAAGTTTTACATCAACAACTTCAACAATTTCATCAACTACAACAATTTNTACTAC
CACAACAGGATCATCANANCATTCAGGATCATCATATTCAGGATCAGTAATTTCAAGTTT
TATCACTTCAATGATTACAAGTGCAAGTGAAAGTTTAAGTGGAAGTGTAAGTGGTAGCAG
CTNTAGNGATAGCAGTAGCTNTAGTGGTAGCAGCTNTAGTGGTAGTAGTTA---------
-
Length of 5' end seq. 411
3' end seq. ID VFC187Z
3' end seq. 
>VFC187Z.Seq
----------TAAATTATTTAAAAAATAATAATTGTAACTATTTATATTCCTGTCACATT
TTTAATGTATGGTATTTATGATGACATTTGCAATGTTAGCAGATTCATTATCAAGTAACT
TTTACTTAAAAACTTAGAGATTTTGTTTCAGTCGCAGATGTTGATTTAACATCAACAGTT
GGAACATTCTTTTTATATTGTGGTTTGGCAGATGTTGGAATTATTAATCCAAAAGAAACA
AAAACTAAAATTATTTTATTATTCTCTTCTAAATGGTGTGCTCACTTTGGTGCTCATTTC
TCTTCAAGTTATGCATGGTTCTTTTTAAGTGATTGTGCTATGCTCTTAATTTCTCATTAT
GTAACTGTTTCAAGAGGTTCCAAATCACCATCATCGCAATCATTCTATAATAAGCAACCA
TTATTATCAAAATTAAATAATGACACTGTTAACAAATCTACAATCATCATTCCATCTGCA
TATAAATCACCAGTTGTGCAATCTTATTTCATTTCTGTACCACAACAAGTACAACCACAA
CAATATCGTTTTGTTCCACAACAACCACAACCAGTTTTTTATGCTAACCAAATTCAACAA
CAACAACAACAAGACAAACCATTAAGTTATATTGTTAAGAAACACTAAGAAATTTAATAA
GAAATTTCAAAAAAAATAATTAAAATAA
Length of 3' end seq. 678
Connected seq. ID VFC187P
Connected seq. 
>VFC187P.Seq
ACTGTTGGCCTACTGGNAACCAATATTTTTTTTTTANATAATTATGGATAATTTTGATTT
TGTATCAGGATTTGGNGCCAGTAGNGGAAGNGGAAGTGGATCAGGNGGTATTTCAACTAT
TTCANGTACTGGNGGTACTGGCCAATCAAGNGGTNCAGTAACTAGTNCTACAAGTNCACC
AACTCCAAGTTTTACATCAACAACTTCAACAATTTCATCAACTACAACAATTTNTACTAC
CACAACAGGATCATCANANCATTCAGGATCATCATATTCAGGATCAGTAATTTCAAGTTT
TATCACTTCAATGATTACAAGTGCAAGTGAAAGTTTAAGTGGAAGTGTAAGTGGTAGCAG
CTNTAGNGATAGCAGTAGCTNTAGTGGTAGCAGCTNTAGTGGTAGTAGTTA---------
-TAAATTATTTAAAAAATAATAATTGTAACTATTTATATTCCTGTCACATTTTTAATGTA
TGGTATTTATGATGACATTTGCAATGTTAGCAGATTCATTATCAAGTAACTTTTACTTAA
AAACTTAGAGATTTTGTTTCAGTCGCAGATGTTGATTTAACATCAACAGTTGGAACATTC
TTTTTATATTGTGGTTTGGCAGATGTTGGAATTATTAATCCAAAAGAAACAAAAACTAAA
ATTATTTTATTATTCTCTTCTAAATGGTGTGCTCACTTTGGTGCTCATTTCTCTTCAAGT
TATGCATGGTTCTTTTTAAGTGATTGTGCTATGCTCTTAATTTCTCATTATGTAACTGTT
TCAAGAGGTTCCAAATCACCATCATCGCAATCATTCTATAATAAGCAACCATTATTATCA
AAATTAAATAATGACACTGTTAACAAATCTACAATCATCATTCCATCTGCATATAAATCA
CCAGTTGTGCAATCTTATTTCATTTCTGTACCACAACAAGTACAACCACAACAATATCGT
TTTGTTCCACAACAACCACAACCAGTTTTTTATGCTAACCAAATTCAACAACAACAACAA
CAAGACAAACCATTAAGTTATATTGTTAAGAAACACTAAGAAATTTAATAAGAAATTTCA
AAAAAAATAATTAAAATAA
Length of connected seq. 1089
Full length Seq ID -
Full length Seq. -
Length of full length seq. -