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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC162
Library VF
(Link to library)
Clone ID VFC162
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFC162P
(Link to Original site)
Representative DNA sequence 
>VFC162 (VFC162Q) /CSM/VF/VFC1-C/VFC162Q.Seq.d/
CACACAATAAATATATACACACATAATAAATATACACATAATAAACAACCATGGGTAAAG
AGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCA
CTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAA
AGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAA
AGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCA
AATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTA
CTGGTACXXXXXXXXXXGTCCAGGTGACAAATGTAGGTTTCAACGTTAAAAACGTTTCAG
TCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAA
CTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATTCATGCTGGTT
ACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCG
ATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCT
TAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAAT
CCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCG
CCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAG
GTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
hnkyiht**iyt**TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEK
EASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMIT
G---

---VQVTNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSP
VLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFT
EYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*inn
Translated Amino Acid sequence (All Frames) 
Frame A:
htiniythnkythnkqpwvkrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksmk
rkllkwvnnpsntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*l
lv---

---VQVTNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSP
VLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFT
EYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*inn

Frame B:
tq*iythiinihiinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*k
gsf*ng*tilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeydy
wy---

---sr*qm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvkfmlvthq
s*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnpsl
nthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*i

Frame C:
hnkyiht**iyt**TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEK
EASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMIT
G---

---pgdkcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnscwllts
lrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilh*
iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeink*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC162 (VFC162Q) /CSM/VF/VFC1-C/VFC162Q.Seq.d/                       1588   0.0
VFG453 (VFG453Q) /CSM/VF/VFG4-C/VFG453Q.Seq.d/                        979   0.0
SLB115 (SLB115Q) /CSM/SL/SLB1-A/SLB115Q.Seq.d/                        977   0.0
VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                        969   0.0
VSD312 (VSD312Q) /CSM/VS/VSD3-A/VSD312Q.Seq.d/                        969   0.0
VFN474 (VFN474Q) /CSM/VF/VFN4-D/VFN474Q.Seq.d/                        969   0.0
VFN472 (VFN472Q) /CSM/VF/VFN4-C/VFN472Q.Seq.d/                        969   0.0
VFN448 (VFN448Q) /CSM/VF/VFN4-B/VFN448Q.Seq.d/                        969   0.0
VFN415 (VFN415Q) /CSM/VF/VFN4-A/VFN415Q.Seq.d/                        969   0.0
VFN340 (VFN340Q) /CSM/VF/VFN3-B/VFN340Q.Seq.d/                        969   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   918  0.0  3
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   896  0.0  3
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   698  0.0  4
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   180  e-130  6
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   250  e-127  5
AX595858|AX595858.1 Sequence 1512 from Patent EP1258494.         103  e-103  6
AX111255|AX111255.1 Sequence 1988 from Patent WO0123604.         103  e-103  6
AX111254|AX111254.1 Sequence 1987 from Patent WO0123604.         103  e-103  6
AX109932|AX109932.1 Sequence 665 from Patent WO0123604.          103  e-103  6
X00779|X00779.1 Yeast gene for elongation factor 1-alpha (EF-1-alpha).   103  e-103  6
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   280   6e-74
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   280   6e-74
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   280   6e-74
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   214   4e-54
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   213   7e-54
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   213   7e-54
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   213   9e-54
FN392319_1522(FN392319|pid:none) Pichia pastoris GS115 chromosom...   212   1e-53
(P40911) RecName: Full=Elongation factor 1-alpha;          Short...   212   2e-53
AB326305_1(AB326305|pid:none) Solea senegalensis SseEF1A4 mRNA f...   211   2e-53
protein update 2009. 6.17
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFC162 is nuc
5' end seq. ID VFC162F
5' end seq. 
>VFC162F.Seq
CACACAATAAATATATACACACATAATAAATATACACATAATAAACAACCATGGGTAAAG
AGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCA
CTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAA
AGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAA
AGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCA
AATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTA
CTGGTAC----------
Length of 5' end seq. 367
3' end seq. ID VFC162Z
3' end seq. 
>VFC162Z.Seq
----------GTCCAGGTGACAAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGA
AATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAA
ATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATTCATGCTGGTTACTCACC
AGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGT
TGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAA
TGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCAC
TGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGG
TGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGC
TGCCCCATCAAAAAAGAAATAAATAAATAAT
Length of 3' end seq. 501
Connected seq. ID VFC162P
Connected seq. 
>VFC162P.Seq
CACACAATAAATATATACACACATAATAAATATACACATAATAAACAACCATGGGTAAAG
AGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCA
CTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAA
AGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAA
AGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCA
AATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTA
CTGGTAC----------GTCCAGGTGACAAATGTAGGTTTCAACGTTAAAAACGTTTCAG
TCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAA
CTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATTCATGCTGGTT
ACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCG
ATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCT
TAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAAT
CCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCG
CCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAG
GTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAAT
Length of connected seq. 868
Full length Seq ID -
Full length Seq. -
Length of full length seq. -