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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC144
Library VF
(Link to library)
Clone ID VFC144
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFC144P
(Link to Original site)
Representative DNA sequence 
>VFC144 (VFC144Q) /CSM/VF/VFC1-B/VFC144Q.Seq.d/
ACTGTTGGCCTACTGGAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTT
TAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCC
ATCAAGCTTCAAGAGAATCTAGAAGGAAGAGTTCCACAAAAATGGTCAAACAGGATTATC
TGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGG
TGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATC
CAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAA
CAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCA
ATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGXXXXXXXXXXTGGTTATCAAGA
ACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACT
GGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCTTTATGGATGAC
TTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGAT
TCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTA
GGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTA
CCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTT
TTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTA
AACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCCTCTGCTTACTATACTGTATT
CGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTNCAAGGTTAAATTTTTTAAT
TAATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywnchltyikeneityfnfifsyycfsssfnstiklpssfkri*KEEFHKNGQTGLS
ALNAGTTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHN
KYNSGASSTYVANGTDFTIQYGSGAMSGFVSQ---

---WLSRTPGANGGELSFGSIDNTKYTGDITYVPLTNETYWEFFMDDFAIDGQSAGFCGT
TCHAICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFV
LTPKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFILCLLYCIRFW**tswfrnch
xkvkffn*
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllelspdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenlegrvpqkwsnrii
csqcwyhnpnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*
qi*qrclkhicrqrn*fhhpir*wcyvrfcls---

---WLSRTPGANGGELSFGSIDNTKYTGDITYVPLTNETYWEFFMDDFAIDGQSAGFCGT
TCHAICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFV
LTPKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFILCLLYCIRFW**tswfrnch
xkvkffn*

Frame B:
llaywnchltyikeneityfnfifsyycfsssfnstiklpssfkri*KEEFHKNGQTGLS
ALNAGTTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHN
KYNSGASSTYVANGTDFTIQYGSGAMSGFVSQ---

---gyqelqvptvvnshsvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvl
lvtqfaiqvhhsllvqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf
*lqkntf*kllsserlnv*vdlwvss*tweisgslvmfsssayytvfdfgnkqvgfatai
xrlnflin

Frame C:
cwptgivt*lt*kkmklliltlflativlaqaltvplnfhqasresrrksstkmvkqdyl
lsmlvpqsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyit
nitavpqahmsptelispsntvvvlcqvlslk---

---viknsrcqrw*tlirfnr*hqihw*hylrpinqrnllgilyg*lcyrwsiswflwyy
lsrnlrfryithcwsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icf
nskrirfrsy*vrkd*mfewiygyrvkhgkfldpw*cfhplltilysilvinklvsqlpx
qg*if*li
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC144 (VFC144Q) /CSM/VF/VFC1-B/VFC144Q.Seq.d/                       1966   0.0
VSF331 (VSF331Q) /CSM/VS/VSF3-B/VSF331Q.Seq.d/                       1063   0.0
CHB247 (CHB247Q) /CSM/CH/CHB2-B/CHB247Q.Seq.d/                       1061   0.0
CFC853 (CFC853Q) /CSM/CF/CFC8-C/CFC853Q.Seq.d/                       1061   0.0
CHD888 (CHD888Q) /CSM/CH/CHD8-D/CHD888Q.Seq.d/                       1059   0.0
VFK365 (VFK365Q) /CSM/VF/VFK3-C/VFK365Q.Seq.d/                       1055   0.0
VFJ894 (VFJ894Q) /CSM/VF/VFJ8-D/VFJ894Q.Seq.d/                       1055   0.0
VFD124 (VFD124Q) /CSM/VF/VFD1-A/VFD124Q.Seq.d/                       1055   0.0
VFA140 (VFA140Q) /CSM/VF/VFA1-B/VFA140Q.Seq.d/                       1055   0.0
SFC585 (SFC585Q) /CSM/SF/SFC5-D/SFC585Q.Seq.d/                       1055   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   926  0.0  7
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.   926  0.0  8
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62  1e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  1e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62  1e-05  1
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62  1e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62  1e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  1e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  1e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  1e-05  1
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   161   5e-40
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   148   1e-37
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   146   4e-37
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   146   5e-37
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   147   5e-37
DQ131585_1(DQ131585|pid:none) Opisthorchis viverrini cathepsin D...   144   1e-36
FJ655904_1(FJ655904|pid:none) Rhipicephalus microplus aspartic p...   148   1e-36
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   149   3e-36
AF420068_1(AF420068|pid:none) Clonorchis sinensis aspartic prote...   140   2e-35
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   140   3e-35
protein update 2009. 6.17
PSORT 

 psg: 0.87  gvh: 0.37  alm: 0.29  top: 0.73  tms: 0.07  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VFC144 is cyt
5' end seq. ID VFC144F
5' end seq. 
>VFC144F.Seq
ACTGTTGGCCTACTGGAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTT
TAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCC
ATCAAGCTTCAAGAGAATCTAGAAGGAAGAGTTCCACAAAAATGGTCAAACAGGATTATC
TGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGG
TGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATC
CAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAA
CAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCA
ATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAG----------
Length of 5' end seq. 458
3' end seq. ID VFC144Z
3' end seq. 
>VFC144Z.Seq
----------TGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTC
AATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTA
TTGGGAATTCTTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTAC
TACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATAT
TACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGA
TTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGT
TTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAG
TGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCAT
CCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCA
TTNCAAGGTTAAATTTTTTAATTAATT
Length of 3' end seq. 557
Connected seq. ID VFC144P
Connected seq. 
>VFC144P.Seq
ACTGTTGGCCTACTGGAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTT
TAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCC
ATCAAGCTTCAAGAGAATCTAGAAGGAAGAGTTCCACAAAAATGGTCAAACAGGATTATC
TGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGG
TGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATC
CAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAA
CAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCA
ATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAG----------TGGTTATCAAGA
ACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACT
GGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCTTTATGGATGAC
TTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGAT
TCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTA
GGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTA
CCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTT
TTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTA
AACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCCTCTGCTTACTATACTGTATT
CGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTNCAAGGTTAAATTTTTTAAT
TAATT
Length of connected seq. 1015
Full length Seq ID -
Full length Seq. -
Length of full length seq. -