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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC129
Library VF
(Link to library)
Clone ID VFC129
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16543-1
Original site URL
Representative seq. ID VFC129F
(Link to Original site)
Representative DNA sequence 
>VFC129 (VFC129Q) /CSM/VF/VFC1-B/VFC129Q.Seq.d/
ACTGTTGGCCTACTGGTACAATCAAATAAATAATTTATATTAAAAAATGTCCAGGAGTAA
TTCATATTTCATCTAATGAAGAATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTG
ACTTTAGTGCTGCATGGTGTGGTCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTGG
CAAACGAATTTGTAACATTTACATGCCGNTCATGTTGACATTGATAAACTAAGNGGGCAT
CCAATAGNAAAAGAAATTAGAAGGGGGCCTACATTCXXXXXXXXXX
sequence update 2001. 6. 1
Translated Amino Acid sequence 
cwptgtik*IIYIKKCPGVIHISSNEELDKHLQAERLVIDFSAAWCGPCRAISPVFEKLA
NEFVTFTCRSC*h**tkxasnxkrn*kgayi---
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvqsnk*filknvqe*fifhlmkn*inicklkd**ltlvlhgvvhveqylrylksw
qtnl*hlhaxhvdidklxghpixkeirrgpt---


Frame B:
llaywynqinnly*kmsrsnsyfi**rir*tfas*kisd*l*ccmvwsm*snisgi*kag
kricniympxmltlin*xgiq*xkklegglh---


Frame C:
cwptgtik*IIYIKKCPGVIHISSNEELDKHLQAERLVIDFSAAWCGPCRAISPVFEKLA
NEFVTFTCRSC*h**tkxasnxkrn*kgayi---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC129 (VFC129Q) /CSM/VF/VFC1-B/VFC129Q.Seq.d/                        529   e-150
SHB431 (SHB431Q) /CSM/SH/SHB4-B/SHB431Q.Seq.d/                        428   e-119
VSB292 (VSB292Q) /CSM/VS/VSB2-D/VSB292Q.Seq.d/                        410   e-114
SSM341 (SSM341Q) /CSM/SS/SSM3-B/SSM341Q.Seq.d/                        410   e-114
AHQ590 (AHQ590Q) /CSM/AH/AHQ5-D/AHQ590Q.Seq.d/                        410   e-114
AHG261 (AHG261Q) /CSM/AH/AHG2-C/AHG261Q.Seq.d/                        410   e-114
VSJ416 (VSJ416Q) /CSM/VS/VSJ4-A/VSJ416Q.Seq.d/                        406   e-113
SSM584 (SSM584Q) /CSM/SS/SSM5-D/SSM584Q.Seq.d/                        406   e-113
VSC718 (VSC718Q) /CSM/VS/VSC7-A/VSC718Q.Seq.d/                        404   e-112
SSA493 (SSA493Q) /CSM/SS/SSA4-D/SSA493Q.Seq.d/                        404   e-112
own update 2004. 8.28
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M91382|M91382.1 Dictyostelium discoideum thioredoxin (TRX2) mRNA, 5' end.   281  2e-96  3
M91384|M91384.1 Dictyostelium discoideum thioredoxin (TRX1) mRNA, complete cds.   186  2e-66  3
M91383|M91383.1 Dictyostelium discoideum thioredoxin (TRX3) mRNA, complete cds.   168  3e-56  4
Y09539|Y09539.1 L.japonicus gene encoding RING finger protein.    48  0.058  1
BH072553|BH072553.1 RPCI-24-376L14.TV RPCI-24 Mus musculus genomic clone RPCI-24-376L14 , DNA sequence.    48  0.058  1
AC119905|AC119905.3 Mus musculus clone RP24-238N14, WORKING DRAFT SEQUENCE, 6 unordered pieces.    48  0.058  3
BH147335|BH147335.1 ENTQM34TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40  0.12  2
AZ545696|AZ545696.1 ENTDP52TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40  0.12  2
AZ685217|AZ685217.1 ENTIA07TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40  0.12  2
BH137539|BH137539.1 ENTNA83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40  0.12  2
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P29446) RecName: Full=Thioredoxin-2;          Short=Trx-2; Flag...   102   6e-27
(P29447) RecName: Full=Thioredoxin-3;          Short=Trx-3;            88   2e-21
C46264(C46264) thioredoxin 3 - slime mold (Dictyostelium discoid...    88   2e-21
EU706448_1(EU706448|pid:none) Triticum aestivum cultivar Soisson...    52   8e-06
AM920433_354(AM920433|pid:none) Penicillium chrysogenum Wisconsi...    51   1e-05
FN392322_288(FN392322|pid:none) Pichia pastoris GS115 chromosome...    51   1e-05
BC129794_1(BC129794|pid:none) Xenopus laevis hypothetical protei...    50   2e-05
(Q8TFM8) RecName: Full=Thioredoxin-like protein; AltName: Allerg...    50   2e-05
(Q5WNE3) RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)...    50   2e-05
S77780(S77780;S46921) thioredoxin - Mycoplasma capricolum (fragm...    50   3e-05
protein update 2009. 6.17
PSORT 

 psg: 0.85  gvh: 0.65  alm: 0.50  top: 0.57  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: extracellular, including cell wall
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VFC129 is cyt
5' end seq. ID VFC129F
5' end seq. 
>VFC129F.Seq
ACTGTTGGCCTACTGGTACAATCAAATAAATAATTTATATTAAAAAATGTCCAGGAGTAA
TTCATATTTCATCTAATGAAGAATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTG
ACTTTAGTGCTGCATGGTGTGGTCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTGG
CAAACGAATTTGTAACATTTACATGCCGNTCATGTTGACATTGATAAACTAAGNGGGCAT
CCAATAGNAAAAGAAATTAGAAGGGGGCCTACATTC----------
Length of 5' end seq. 276
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -