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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC123
Library VF
(Link to library)
Clone ID VFC123
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15566-1
Original site URL
Representative seq. ID VFC123P
(Link to Original site)
Representative DNA sequence 
>VFC123 (VFC123Q) /CSM/VF/VFC1-A/VFC123Q.Seq.d/
CTGTTGGCCTACTGGGTACAACAATAATAACAACAATCAATAATAATATAATGATGGAAG
ATGAAATTGATATAGATTTTTCAAATGAAATCTCAAGACAAGATGTATTACAATTAAGAG
AATATTATGATAAATTATTTCCATTTGATTTATTATATGATTGGTTAACAATAAATAGTA
TAAATGAAAAAGAAGAAGATAAAAAGAGAGCATTTGAAAGAAGAGAATTTTCAGTAAGGA
TCAATAAAGTATCTTCATCATTTGTTATTCGTAATAAATCATTCAATAGAGTTGAAGATT
TAAAAGAATTTTTAAAAGGTAGACAAAATATTGATGGTGTATATGAAGTACCAGAGAGAT
TAGATATTGGACCCATCTGTAGTTGGGAACCATCTAAAAAGGCAACAGTTTCAGATTACC
AACCAGTGTCAAAGGAATTGGTATTCGATATTGATGTTGATGATTATAATCCAATTCGTG
TTTGTTGTTCATCAACATCACTATGTGAAAAGTGTTGGGGATTTTTAGCAGTTTCAGCAG
AGTTTATAGATAATTATCTTAGGGAATGTTTTGGTTXXXXXXXXXXGTGCTTCAATTTTA
AATCATATCAGAGTTGAGTTATCTCAAAATCAAATCCCAATTTTTAAACGTTCATTCGAC
CAAATTNTATTACCACCCTTTGAAAAATGCATTATANAGGAACAACAAATTTTCACCAAT
GAAAAACAACGTAAAAACTTTTTATCATTATTACCAATNTCTTACAAGAGACAAGATAGA
AAAATCCCTTTAACGCCATCTGAAGATATTGAAAAGAAATTAAAAGAAATTCAAGATGAA
AATTTATCAAACTCTGAAATTTGGTTCGCTGTTAAATCTTATCTCGAAGATAATAATTTA
AAGGAACATCTCTACAATTTAGTTTTCCATTATACAATGCCAAGAATTGATGAACCAGTA
ACGATTGGTCTTGATCATCTTTTGAAATCCCCATTCTGTANACNCCCTGANACAAANAAA
CTTTGNGTTCCCTTGGATATTAATGAAATTTATAAATTTAATCCAAATCAAGTTCCAACA
GTTGATCAATTAATTAATGATCCAAAATTATTTAACAAATTTAAAAAAATATTTAAAAAT
CATGTAAATCAAATGAAATTAAAATATAATTTAACAATCAAGANCCAAGGANTTTAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
VGLLGTTIITTINNNIMMEDEIDIDFSNEISRQDVLQLREYYDKLFPFDLLYDWLTINSI
NEKEEDKKRAFERREFSVRINKVSSSFVIRNKSFNRVEDLKEFLKGRQNIDGVYEVPERL
DIGPICSWEPSKKATVSDYQPVSKELVFDIDVDDYNPIRVCCSSTSLCEKCWGFLAVSAE
FIDNYLRECFG---

---ASILNHIRVELSQNQIPIFKRSFDQIXLPPFEKCIIXEQQIFTNEKQRKNFLSLLPX
SYKRQDRKIPLTPSEDIEKKLKEIQDENLSNSEIWFAVKSYLEDNNLKEHLYNLVFHYTM
PRIDEPVTIGLDHLLKSPFCXXPXTXKLXVPLDINEIYKFNPNQVPTVDQLINDPKLFNK
FKKIFKNHVNQMKLKYNLTIKXQGX*
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywvqq**qqsiii**wkmkli*ifqmksqdkmyyn*eniminyfhliyymig*q*iv
*mkkkkikrehlkeenfq*gsikylhhllfvinhsielki*knf*kvdkilmvymkyqrd
*ildpsvvgnhlkrqqfqitnqcqrnwysilmlmiiiqfvfvvhqhhyvksvgdf*qfqq
sl*iiilgnvlv---

---vlqf*iiselsylkiksqflnvhstkxyyhplknalxrnnkfspmknnvktfyhyyq
xltrdkieksl*rhlkilkrn*kkfkmkiyqtlkfgsllnliskiii*rnisti*fsiiq
cqelmnq*rlvliif*nphsvxxlxqxnfxfpwilmkfinliqikfqqlin*lmiqnylt
nlkkylkim*ik*n*nii*qsrxkxfn

Frame B:
cwptgynnnnnnq**yndgr*n*yrffk*nlktrcitikril**iisi*fii*lvnnk*y
k*krrr*kesi*kkrifskdq*sifiicys**iiq*s*rfkrifkr*tky*wci*strei
rywthl*lgti*kgnsfrlptsvkgigiry*c**l*snscllfinitm*kvlgifssfsr
vyr*ls*gmfw---

---cfnfksyqs*visksnpnf*tfirpnxittl*kmhyxgttnfhq*ktt*klfiiitn
xlqetr*knpfnai*ry*keikrnsr*kfikl*nlvrc*ilsrr**fkgtslqfsfplyn
akn**tsndws*ssfeipilxtp*xkxtlxslgy**nl*i*sksssns*sin**skii*q
i*kni*kscksneiki*fnnqxprxl

Frame C:
VGLLGTTIITTINNNIMMEDEIDIDFSNEISRQDVLQLREYYDKLFPFDLLYDWLTINSI
NEKEEDKKRAFERREFSVRINKVSSSFVIRNKSFNRVEDLKEFLKGRQNIDGVYEVPERL
DIGPICSWEPSKKATVSDYQPVSKELVFDIDVDDYNPIRVCCSSTSLCEKCWGFLAVSAE
FIDNYLRECFG---

---ASILNHIRVELSQNQIPIFKRSFDQIXLPPFEKCIIXEQQIFTNEKQRKNFLSLLPX
SYKRQDRKIPLTPSEDIEKKLKEIQDENLSNSEIWFAVKSYLEDNNLKEHLYNLVFHYTM
PRIDEPVTIGLDHLLKSPFCXXPXTXKLXVPLDINEIYKFNPNQVPTVDQLINDPKLFNK
FKKIFKNHVNQMKLKYNLTIKXQGX*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC123 (VFC123Q) /CSM/VF/VFC1-A/VFC123Q.Seq.d/                       2068   0.0
SFE189 (SFE189Q) /CSM/SF/SFE1-D/SFE189Q.Seq.d/                       1047   0.0
AFD446 (AFD446Q) /CSM/AF/AFD4-B/AFD446Q.Seq.d/                        926   0.0
SFH468 (SFH468Q) /CSM/SF/SFH4-C/SFH468Q.Seq.d/                        817   0.0
VSK435 (VSK435Q) /CSM/VS/VSK4-B/VSK435Q.Seq.d/                         48   3e-04
VFH751 (VFH751Q) /CSM/VF/VFH7-C/VFH751Q.Seq.d/                         48   3e-04
VFF822 (VFF822Q) /CSM/VF/VFF8-A/VFF822Q.Seq.d/                         48   3e-04
SHK140 (SHK140Q) /CSM/SH/SHK1-B/SHK140Q.Seq.d/                         48   3e-04
AFO612 (AFO612Q) /CSM/AF/AFO6-A/AFO612Q.Seq.d/                         48   3e-04
SSD428 (SSD428Q) /CSM/SS/SSD4-B/SSD428Q.Seq.d/                         46   0.001
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    34  6e-07  16
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    34  2e-04  15
AP003191|AP003191.2 Clostridium perfringens DNA, complete genome, section 7/10.    36  0.002  11
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    42  0.002  12
AC015522|AC015522.6 Homo sapiens chromosome 8, clone RP11-327O12, complete sequence.    36  0.002  6
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          42  0.006  14
AC104940|AC104940.2 Homo sapiens chromosome 8, clone RP11-378J9, complete sequence.    36  0.009  6
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    32  0.010  14
AF267201|AF267201.1 Candidatus Carsonella ruddii natural-host Bactericera cockerelli ATP synthase alpha subunit (atpA) gene, partial cds; ATP synthase gamma subunit (atpG) gene, complete cds; and ATP synthase beta subunit (atpD) gene, partial cds.    34  0.016  4
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    32  0.022  15
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF403474_1(AF403474|pid:none) Euplotes crassus DNA primase catal...   109   2e-22
AE017345_174(AE017345|pid:none) Cryptococcus neoformans var. neo...   105   3e-21
CR382128_437(CR382128|pid:none) Yarrowia lipolytica strain CLIB1...   103   1e-20
AB072454_1(AB072454|pid:none) Coprinopsis cinerea mRNA for DNA p...   102   2e-20
BC153661_1(BC153661|pid:none) Danio rerio primase polypeptide 1,...   100   1e-19
AK223391_1(AK223391|pid:none) Homo sapiens mRNA for DNA primase ...    99   4e-19
(P49642) RecName: Full=DNA primase small subunit;          EC=2....    99   4e-19
AK133878_1(AK133878|pid:none) Mus musculus 8 days embryo whole b...    98   7e-19
(P20664) RecName: Full=DNA primase small subunit;          EC=2....    98   7e-19
BC049395_1(BC049395|pid:none) Xenopus laevis primase, polypeptid...    97   9e-19
protein update 2009. 6.17
PSORT 

 psg: 0.92  gvh: 0.37  alm: 0.44  top: 0.13  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFC123 is cyt
5' end seq. ID VFC123F
5' end seq. 
>VFC123F.Seq
CTGTTGGCCTACTGGGTACAACAATAATAACAACAATCAATAATAATATAATGATGGAAG
ATGAAATTGATATAGATTTTTCAAATGAAATCTCAAGACAAGATGTATTACAATTAAGAG
AATATTATGATAAATTATTTCCATTTGATTTATTATATGATTGGTTAACAATAAATAGTA
TAAATGAAAAAGAAGAAGATAAAAAGAGAGCATTTGAAAGAAGAGAATTTTCAGTAAGGA
TCAATAAAGTATCTTCATCATTTGTTATTCGTAATAAATCATTCAATAGAGTTGAAGATT
TAAAAGAATTTTTAAAAGGTAGACAAAATATTGATGGTGTATATGAAGTACCAGAGAGAT
TAGATATTGGACCCATCTGTAGTTGGGAACCATCTAAAAAGGCAACAGTTTCAGATTACC
AACCAGTGTCAAAGGAATTGGTATTCGATATTGATGTTGATGATTATAATCCAATTCGTG
TTTGTTGTTCATCAACATCACTATGTGAAAAGTGTTGGGGATTTTTAGCAGTTTCAGCAG
AGTTTATAGATAATTATCTTAGGGAATGTTTTGGTT----------
Length of 5' end seq. 576
3' end seq. ID VFC123Z
3' end seq. 
>VFC123Z.Seq
----------GTGCTTCAATTTTAAATCATATCAGAGTTGAGTTATCTCAAAATCAAATC
CCAATTTTTAAACGTTCATTCGACCAAATTNTATTACCACCCTTTGAAAAATGCATTATA
NAGGAACAACAAATTTTCACCAATGAAAAACAACGTAAAAACTTTTTATCATTATTACCA
ATNTCTTACAAGAGACAAGATAGAAAAATCCCTTTAACGCCATCTGAAGATATTGAAAAG
AAATTAAAAGAAATTCAAGATGAAAATTTATCAAACTCTGAAATTTGGTTCGCTGTTAAA
TCTTATCTCGAAGATAATAATTTAAAGGAACATCTCTACAATTTAGTTTTCCATTATACA
ATGCCAAGAATTGATGAACCAGTAACGATTGGTCTTGATCATCTTTTGAAATCCCCATTC
TGTANACNCCCTGANACAAANAAACTTTGNGTTCCCTTGGATATTAATGAAATTTATAAA
TTTAATCCAAATCAAGTTCCAACAGTTGATCAATTAATTAATGATCCAAAATTATTTAAC
AAATTTAAAAAAATATTTAAAAATCATGTAAATCAAATGAAATTAAAATATAATTTAACA
ATCAAGANCCAAGGANTTTAAT
Length of 3' end seq. 612
Connected seq. ID VFC123P
Connected seq. 
>VFC123P.Seq
CTGTTGGCCTACTGGGTACAACAATAATAACAACAATCAATAATAATATAATGATGGAAG
ATGAAATTGATATAGATTTTTCAAATGAAATCTCAAGACAAGATGTATTACAATTAAGAG
AATATTATGATAAATTATTTCCATTTGATTTATTATATGATTGGTTAACAATAAATAGTA
TAAATGAAAAAGAAGAAGATAAAAAGAGAGCATTTGAAAGAAGAGAATTTTCAGTAAGGA
TCAATAAAGTATCTTCATCATTTGTTATTCGTAATAAATCATTCAATAGAGTTGAAGATT
TAAAAGAATTTTTAAAAGGTAGACAAAATATTGATGGTGTATATGAAGTACCAGAGAGAT
TAGATATTGGACCCATCTGTAGTTGGGAACCATCTAAAAAGGCAACAGTTTCAGATTACC
AACCAGTGTCAAAGGAATTGGTATTCGATATTGATGTTGATGATTATAATCCAATTCGTG
TTTGTTGTTCATCAACATCACTATGTGAAAAGTGTTGGGGATTTTTAGCAGTTTCAGCAG
AGTTTATAGATAATTATCTTAGGGAATGTTTTGGTT----------GTGCTTCAATTTTA
AATCATATCAGAGTTGAGTTATCTCAAAATCAAATCCCAATTTTTAAACGTTCATTCGAC
CAAATTNTATTACCACCCTTTGAAAAATGCATTATANAGGAACAACAAATTTTCACCAAT
GAAAAACAACGTAAAAACTTTTTATCATTATTACCAATNTCTTACAAGAGACAAGATAGA
AAAATCCCTTTAACGCCATCTGAAGATATTGAAAAGAAATTAAAAGAAATTCAAGATGAA
AATTTATCAAACTCTGAAATTTGGTTCGCTGTTAAATCTTATCTCGAAGATAATAATTTA
AAGGAACATCTCTACAATTTAGTTTTCCATTATACAATGCCAAGAATTGATGAACCAGTA
ACGATTGGTCTTGATCATCTTTTGAAATCCCCATTCTGTANACNCCCTGANACAAANAAA
CTTTGNGTTCCCTTGGATATTAATGAAATTTATAAATTTAATCCAAATCAAGTTCCAACA
GTTGATCAATTAATTAATGATCCAAAATTATTTAACAAATTTAAAAAAATATTTAAAAAT
CATGTAAATCAAATGAAATTAAAATATAATTTAACAATCAAGANCCAAGGANTTTAAT
Length of connected seq. 1188
Full length Seq ID -
Full length Seq. -
Length of full length seq. -