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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC115
Library VF
(Link to library)
Clone ID VFC115
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFC115E
(Link to Original site)
Representative DNA sequence 
>VFC115 (VFC115Q) /CSM/VF/VFC1-A/VFC115Q.Seq.d/
ACTGTTGGCCTACTGGTCTATAATATATAAAATACAATAAAAAAATGGACGGAGAAGATG
TTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATG
ATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGG
TCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCT
TAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAA
TCTGGCATCATACCTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCT
TAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTG
AAACTTTCAACACCCCAGCTATGTACGTTGCCATTCAAGCCGTCTTATCCTTATATGCCT
CTGGTCGTACCACTGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAA
TCTATGAAGGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGAT
CTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCT
GAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAA
GCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGAC
GGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCA
TCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAA
TGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATNCTGGTGGTACAAC
TATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAAC
CATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTNCTGGATTGGTGGAT
CTATCTTAGCTTCACTNCTCAACTTTCCAACAAATGTGGATNCNCCAAAGAAGAATATGA
CGAATCAGGTNCCATCAATTGTNCCACAGAAAATGTTTNCTAAACAATTAATA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
tvgllvyni*ntikkwtekmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlw
svwvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiipstmnsvlpqkntqfs
*lkph*iqrptekk*PKLCLKLSTPQLCTLPFKPSYPYMPLVVPLVLLWIQVMVSPTLYQ
SMKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFE
AEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMK
CDVDIRKDLYGNVVLXWWYNYVPRYC*syeqrincfspinhen*nhcstrt*ilcxglvd
ls*lhxstfqqmwxxqrri*rirxhqlxhrkcxlnn*
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvyni*ntikkwtekmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlw
svwvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiipstmnsvlpqkntqfs
*lkph*iqrptekk*PKLCLKLSTPQLCTLPFKPSYPYMPLVVPLVLLWIQVMVSPTLYQ
SMKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFE
AEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMK
CDVDIRKDLYGNVVLXWWYNYVPRYC*syeqrincfspinhen*nhcstrt*ilcxglvd
ls*lhxstfqqmwxxqrri*rirxhqlxhrkcxlnn*


Frame B:
llaywsiiykiq*kngrrrcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcyg
rygskrlicr**spikerylnpqipn*tryryqlg*ygknlasyllq*tpccprrtpssl
n*spikskgqqrkndpnyv*nfqhpsyvrchssrliliclwsyhwycygfr*wclphctn
l*rvmpyhmpsyv*i*lvvillit**ks*lnvvthsppllkeksseiskkn*ptspstlk
lkckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnqlvstkppttps*n
vmlisvkiytvmssyxggttmfpgiadrmnkeltalapstmkikiiapperkysvldwwi
ylsftxqlsnkcgxxkeeydesgxincxtenvx*tin


Frame C:
cwptgl*yikynkkmdgedvqalvidngsgmckagfagddapravfpsivgrprhtgvmv
gmgqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfynelrvapeehpvll
teaplnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgivmdsgdgvshtvpi
yeglclttchltfrfsws*sy*lhdenln*twllihhhc*krnrqryqrkislrrprl*s
*nanccliisprkiiritrrsshhhr**tfplprsplptiilrygiswyprnhlqlhhem
*c*yp*rfir*crlxlvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlxwiggs
ilasllnfptnvdxpkknmtnqvpsivpqkmfxkqli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC115 (VFC115Q) /CSM/VF/VFC1-A/VFC115Q.Seq.d/                       2276   0.0
VFL407 (VFL407Q) /CSM/VF/VFL4-A/VFL407Q.Seq.d/                       2163   0.0
VFL115 (VFL115Q) /CSM/VF/VFL1-A/VFL115Q.Seq.d/                       2159   0.0
VFK793 (VFK793Q) /CSM/VF/VFK7-D/VFK793Q.Seq.d/                       2159   0.0
VFI535 (VFI535Q) /CSM/VF/VFI5-B/VFI535Q.Seq.d/                       2159   0.0
VFI284 (VFI284Q) /CSM/VF/VFI2-D/VFI284Q.Seq.d/                       2159   0.0
VFG715 (VFG715Q) /CSM/VF/VFG7-A/VFG715Q.Seq.d/                       2159   0.0
VFE672 (VFE672Q) /CSM/VF/VFE6-C/VFE672Q.Seq.d/                       2159   0.0
VFD830 (VFD830Q) /CSM/VF/VFD8-B/VFD830Q.Seq.d/                       2159   0.0
VFD260 (VFD260Q) /CSM/VF/VFD2-C/VFD260Q.Seq.d/                       2159   0.0
own update 2001.12. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      954  0.0  7
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   906  0.0  9
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        890  0.0  7
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   890  0.0  7
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   866  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   827  0.0  9
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   787  0.0  12
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   763  0.0  8
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     716  0.0  7
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   716  0.0  7
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          341   0.0
AY294153_1(AY294153|pid:none) Platyamoeba placida actin mRNA, pa...   338   0.0
AY294156_1(AY294156|pid:none) Pelomyxa palustris actin mRNA, com...   336   e-178
AY813805_1(AY813805|pid:none) Schistosoma japonicum SJCHGC06318 ...   333   e-173
FN316964_1(FN316964|pid:none) Schistosoma japonicum isolate Anhu...   333   e-173
AY294155_1(AY294155|pid:none) Glaeseria mira actin mRNA, partial...   329   e-173
AF284835_1(AF284835|pid:none) Guillardia theta actin mRNA, parti...   318   e-173
(P53689) RecName: Full=Actin;  &A73764_1(A73764|pid:none) &S7037...   332   e-172
DQ980439_1(DQ980439|pid:none) Leucocryptos marina clone lmactin1...   309   e-172
DQ980440_1(DQ980440|pid:none) Leucocryptos marina clone lmactin1...   307   e-171
protein update 2009. 7.10
PSORT 

 psg: 0.84  gvh: 0.51  alm: 0.27  top: 0.50  tms: 0.07  mit: 0.43  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.42  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 16.0 %: Golgi
	 12.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VFC115 is end
5' end seq. ID VFC115F
5' end seq. 
>VFC115F.Seq
ACTGTTGGCCTACTGGTCTATAATATATAAAATACAATAAAAAAATGGACGGAGAAGATG
TTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATG
ATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGG
TCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCT
TAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAA
TCTGGCATCATACCTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCT
TAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTG
AAACTTTCAACACCCCAGCTATGTACGTTGCCATTCAAGCCGTCTTATCCTTATATGCCT
CTGGTCGTACCACTGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAA
TCTATGAAGGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGAT
CTTACTGATTA----------
Length of 5' end seq. 611
3' end seq. ID VFC115Z
3' end seq. 
>VFC115Z.Seq
----------CACTGTACCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTT
TAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTT
ACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCT
ACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAA
AATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCC
CAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCA
CCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCG
TCTTATNCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTA
ACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATAC
TCTGTNCTGGATTGGTGGATCTATCTTAGCTTCACTNCTCAACTTTCCAACAAATGTGGA
TNCNCCAAAGAAGAATATGACGAATCAGGTNCCATCAATTGTNCCACAGAAAATGTTTNC
TAAACAATTAATA
Length of 3' end seq. 663
Connected seq. ID VFC115P
Connected seq. 
>VFC115P.Seq
ACTGTTGGCCTACTGGTCTATAATATATAAAATACAATAAAAAAATGGACGGAGAAGATG
TTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATG
ATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGG
TCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCT
TAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAA
TCTGGCATCATACCTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCT
TAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTG
AAACTTTCAACACCCCAGCTATGTACGTTGCCATTCAAGCCGTCTTATCCTTATATGCCT
CTGGTCGTACCACTGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAA
TCTATGAAGGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGAT
CTTACTGATTA----------CACTGTACCAATCTATGAAGGTTATGCCTTACCACATGC
CATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAAC
TGAACGTGGTTACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGA
AAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATC
AGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACG
TTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTAT
CCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATA
CGGTAATGTCGTCTTATNCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGA
ACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAG
AACGTAAATACTCTGTNCTGGATTGGTGGATCTATCTTAGCTTCACTNCTCAACTTTCCA
ACAAATGTGGATNCNCCAAAGAAGAATATGACGAATCAGGTNCCATCAATTGTNCCACAG
AAAATGTTTNCTAAACAATTAATA
Length of connected seq. 1274
Full length Seq ID VFC115E
Full length Seq. 
>VFC115E.Seq
ACTGTTGGCCTACTGGTCTATAATATATAAAATACAATAAAAAAATGGACGGAGAAGATG
TTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATG
ATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGG
TCGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCT
TAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAA
TCTGGCATCATACCTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCT
TAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTG
AAACTTTCAACACCCCAGCTATGTACGTTGCCATTCAAGCCGTCTTATCCTTATATGCCT
CTGGTCGTACCACTGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAA
TCTATGAAGGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGAT
CTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCT
GAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAA
GCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGAC
GGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCA
TCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAA
TGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATNCTGGTGGTACAAC
TATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAAC
CATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTNCTGGATTGGTGGAT
CTATCTTAGCTTCACTNCTCAACTTTCCAACAAATGTGGATNCNCCAAAGAAGAATATGA
CGAATCAGGTNCCATCAATTGTNCCACAGAAAATGTTTNCTAAACAATTAATA
Length of full length seq. 1193