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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFC102
Library VF
(Link to library)
Clone ID VFC102
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFC102P
(Link to Original site)
Representative DNA sequence 
>VFC102 (VFC102Q) /CSM/VF/VFC1-A/VFC102Q.Seq.d/
ACTGTTGGCCTACTGGGTTATAATCAACTTTAAAGNAAAGNAATAAACAACCATGGGTAA
AGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCAC
CACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGA
AAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATT
AAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTC
CAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGAT
TACTGGTACCTCCCAAGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATT
CGAAGCTGGTATCXXXXXXXXXXTGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGA
AGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGT
AGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGA
CTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAA
CCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACAT
TGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTCGC
CAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAAC
CCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGC
CGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGC
CCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATGNAATAA
ATAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywviinfkxkx*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYE
KEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMI
TGTSQADCAVLVIASPTGEFEAGI---

---VVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDP
PQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTA
AVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAG
DKKGAAAPSKKKXNK*
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllgynql*xkxinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*
kgsf*ng*tilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeyd
ywylpsrlcrlshclpnw*irswy---

---wssplpqlvsqlksnqskcitnnsqkpvqvtm*vstlktfqskklkevwslvtpkti
hhkklknsllklssstiqvksmlvthqs*ivtlltlpvnslkssiklivvlvpsspkkvl
ppss*rmvmplwsn*phlvqcvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkp
virkvllphqkrnxinn

Frame B:
llaywviinfkxkx*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYE
KEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMI
TGTSQADCAVLVIASPTGEFEAGI---

---grhlcpswsln*sqisrnasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrs
ttrn*kircssyrpqpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrrqrryc
rrrlkew*crygrinpissnvc*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr
**ercccpikkemx*i

Frame C:
cwptgl*stlkxxnkqpwvkrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksmk
rkllkwvnnpsntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*l
lvppkpivps*slppqlvnsklv---

---VVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDP
PQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTA
AVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAG
DKKGAAAPSKKKXNK*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC102 (VFC102Q) /CSM/VF/VFC1-A/VFC102Q.Seq.d/                       1955   0.0
VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                       1124   0.0
VSD312 (VSD312Q) /CSM/VS/VSD3-A/VSD312Q.Seq.d/                       1124   0.0
VFN474 (VFN474Q) /CSM/VF/VFN4-D/VFN474Q.Seq.d/                       1124   0.0
VFN472 (VFN472Q) /CSM/VF/VFN4-C/VFN472Q.Seq.d/                       1124   0.0
VFN448 (VFN448Q) /CSM/VF/VFN4-B/VFN448Q.Seq.d/                       1124   0.0
VFN415 (VFN415Q) /CSM/VF/VFN4-A/VFN415Q.Seq.d/                       1124   0.0
VFN340 (VFN340Q) /CSM/VF/VFN3-B/VFN340Q.Seq.d/                       1124   0.0
VFN318 (VFN318Q) /CSM/VF/VFN3-A/VFN318Q.Seq.d/                       1124   0.0
VFN291 (VFN291Q) /CSM/VF/VFN2-D/VFN291Q.Seq.d/                       1124   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1100  0.0  2
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1059  0.0  2
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   872  0.0  3
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   192  e-155  8
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   264  e-154  7
AX111255|AX111255.1 Sequence 1988 from Patent WO0123604.         103  e-125  8
AX109932|AX109932.1 Sequence 665 from Patent WO0123604.          103  e-125  8
X00779|X00779.1 Yeast gene for elongation factor 1-alpha (EF-1-alpha).   103  e-125  8
M10992|M10992.1 Yeast (S.cerevisiae) EF-1-alpha-* (elongation factor 1-alpha) gene (TEF1), complete cds.   103  e-124  8
AX595858|AX595858.1 Sequence 1512 from Patent EP1258494.         103  e-124  8
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   343   4e-93
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   343   4e-93
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   343   4e-93
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   267   4e-70
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   267   5e-70
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   265   1e-69
FN392319_1522(FN392319|pid:none) Pichia pastoris GS115 chromosom...   265   2e-69
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   265   2e-69
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   263   6e-69
AJ549292_1(AJ549292|pid:none) Podocoryne carnea mRNA for elongat...   263   6e-69
protein update 2009. 6.17
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFC102 is cyt
5' end seq. ID VFC102F
5' end seq. 
>VFC102F.Seq
ACTGTTGGCCTACTGGGTTATAATCAACTTTAAAGNAAAGNAATAAACAACCATGGGTAA
AGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCAC
CACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGA
AAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATT
AAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTC
CAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGAT
TACTGGTACCTCCCAAGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATT
CGAAGCTGGTATC----------
Length of 5' end seq. 433
3' end seq. ID VFC102Z
3' end seq. 
>VFC102Z.Seq
----------TGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTC
GAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTT
AAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGAT
CCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAA
ATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTC
ACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTCGCCAAAGAAGGTACT
GCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCA
ATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATG
AGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCC
GGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATGNAATAAATAAT
Length of 3' end seq. 582
Connected seq. ID VFC102P
Connected seq. 
>VFC102P.Seq
ACTGTTGGCCTACTGGGTTATAATCAACTTTAAAGNAAAGNAATAAACAACCATGGGTAA
AGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCAC
CACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGA
AAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATT
AAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTC
CAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGAT
TACTGGTACCTCCCAAGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATT
CGAAGCTGGTATC----------TGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGA
AGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGT
AGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGA
CTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAA
CCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACAT
TGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTCGC
CAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAAC
CCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGC
CGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGC
CCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATGNAATAA
ATAAT
Length of connected seq. 1015
Full length Seq ID -
Full length Seq. -
Length of full length seq. -