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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB893
Library VF
(Link to library)
Clone ID VFB893
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15009-1
Original site URL
Representative seq. ID VFB893P
(Link to Original site)
Representative DNA sequence 
>VFB893 (VFB893Q) /CSM/VF/VFB8-D/VFB893Q.Seq.d/
CACTGTTGGCCTACTGGNATTTTTTAATTTAATTTTAATTTATTTTTATTTATATATATA
CACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATTTTATCTTGTATTT
TTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAATTTCAAATGGTGGG
GCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAATGGTAAAGCGGAT
TATAATGTNGAACATTTTTTCAGAGGTGAACATACAAATAATNGGGCACTTTTAGTTTGC
ACATCAAGANTNTGGTTTAACTATAGACATATAGCAAXXXXXXXXXXAAGATATCATGAA
ATTTTATTCATGGTTGATACTTGTCAAGCTAATACATTATATACTCGTTTTAATTCTCCA
AATATTTTAGCAATTGGTTCAAGTAAATTTGGTGAAAATAGTTATTCTCATCATTCAGAT
CCAGAATTAGGGGTTGCAGTTATTGATAGATTTACATATTATACATTGGAATTCTTTGAA
AGTGTTGACCCACATAATGTAACATTATATCAATTGTTCAATACATATAGTCCACAAAAA
TTACAATCACATAGTGAATATAGGACAGATTTATTCAATAGACCATTAGATCAAGTTCCT
GTTACAGACTTTTTTGGTTCTGTAATGAGAGCTGATTTAACTCCTTCAACAATTGTAAAT
GAAAAACAAAAAGATGAAAAAGTAGAAAAAGATGAAACTACCGTAAATAATAATAATAAT
AATAATAACAACGATAATGTAAAAAAACAAACACCAAAAACTAAACAAATTTCATATAGA
ATTACAGAGAAACCAACATCAATCCAAGTACAAAATAACAAAGATAGTGAATTAAATCTT
TTCAATTCAAAATTTATTACTTCAGCTTTAATATTTTTAGTAGTATTTGGATTACTTTCT
TGGTCTTCTGATTTTGTAAAATCAAATCTTATTTTAAATACAAATNATTTAAAAGATCTG
AAATCAAAATCAAAGCAAATTTAAGTAATATATAAATAAATAAATAATAAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
hcwptgif*fnfnlflfiyihttikk*KMKKTTFRFYLVFLYAILVLYIGGIEDAISNGG
AMGYEQPKTNFYNGGNGKADYNVEHFFRGEHTNNXALLVCTSRXWFNYRHIA---

---RYHEILFMVDTCQANTLYTRFNSPNILAIGSSKFGENSYSHHSDPELGVAVIDRFTY
YTLEFFESVDPHNVTLYQLFNTYSPQKLQSHSEYRTDLFNRPLDQVPVTDFFGSVMRADL
TPSTIVNEKQKDEKVEKDETTVNNNNNNNNNDNVKKQTPKTKQISYRITEKPTSIQVQNN
KDSELNLFNSKFITSALIFLVVFGLLSWSSDFVKSNLILNTNXLKDLKSKSKQI*viyk*
innk*
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgif*fnfnlflfiyihttikk*KMKKTTFRFYLVFLYAILVLYIGGIEDAISNGG
AMGYEQPKTNFYNGGNGKADYNVEHFFRGEHTNNXALLVCTSRXWFNYRHIA---

---kis*nfihg*ylss*yiiysf*fskyfsnwfk*iw*k*lfssfrsrirgcsy**iyi
lyigil*kc*pt*cniisivqyi*stkitit**i*drfiq*tirssscyrlfwfcnes*f
nsfnnck*ktkr*ksrkr*nyrk*******qr*ckktntkn*tnfi*nyretninpstk*
qr**iksfqfkiyyfsfnifssiwitflvf*fckiksyfkykxfkrseikikanlsni*i
nk**i

Frame B:
tvgllxffnliliyfylyiytqq*rnkr*kkrhldfilyfymqy*fyi*vvlkmqfqmvg
rwdmnnqkqtfimvvmvkriimxnifsevniqixghf*fahqxxgltidi*q---

---RYHEILFMVDTCQANTLYTRFNSPNILAIGSSKFGENSYSHHSDPELGVAVIDRFTY
YTLEFFESVDPHNVTLYQLFNTYSPQKLQSHSEYRTDLFNRPLDQVPVTDFFGSVMRADL
TPSTIVNEKQKDEKVEKDETTVNNNNNNNNNDNVKKQTPKTKQISYRITEKPTSIQVQNN
KDSELNLFNSKFITSALIFLVVFGLLSWSSDFVKSNLILNTNXLKDLKSKSKQI*viyk*
innk*

Frame C:
llaywxfli*f*fifiyiythnnkeikdekndi*ilscificnisfiyrwy*rcnfkwwg
dgi*ttknkll*ww*w*sgl*cxtffqr*tyk*xgtfslhikxxv*l*tys---

---dimkfyswlilvklihyilvlilqif*qlvqvnlvkiviliiqiqn*glqllidlhi
ihwnslkvlthim*hyincsihivhknynhivnigqiysidh*ikfllqtflvl**eli*
llqql*mknkkmkk*kkmklp*iiiiiiittim*knkhqklnkfhielqrnqhqskykit
kivn*ifsiqnlllql*yf**yldyflgllil*nqilf*iqxi*ki*nqnqskfk*yink
*iin
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB893 (VFB893Q) /CSM/VF/VFB8-D/VFB893Q.Seq.d/                       1189   0.0
SFD257 (SFD257Q) /CSM/SF/SFD2-C/SFD257Q.Seq.d/                        729   0.0
CHD461 (CHD461Q) /CSM/CH/CHD4-C/CHD461Q.Seq.d/                        729   0.0
CFF820 (CFF820Q) /CSM/CF/CFF8-A/CFF820Q.Seq.d/                        729   0.0
AFJ746 (AFJ746Q) /CSM/AF/AFJ7-B/AFJ746Q.Seq.d/                        729   0.0
SFL282 (SFL282Q) /CSM/SF/SFL2-D/SFL282Q.Seq.d/                        724   0.0
CFB364 (CFB364Q) /CSM/CF/CFB3-C/CFB364Q.Seq.d/                        724   0.0
SFJ248 (SFJ248Q) /CSM/SF/SFJ2-B/SFJ248Q.Seq.d/                        682   0.0
CFH296 (CFH296Q) /CSM/CF/CFH2-D/CFH296Q.Seq.d/                        599   e-170
CFH352 (CFH352Q) /CSM/CF/CFH3-C/CFH352Q.Seq.d/                        535   e-151
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX488980|AX488980.1 Sequence 6280 from Patent WO02053728.         52  8e-12  3
BI507800|BI507800.1 BB170001B10H11.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170001B10H11 5', mRNA sequence.    60  6e-05  1
AP004512|AP004512.1 Lotus japonicus genomic DNA, chromosome 4, clone:LjT10L16, TM0042.    38  0.041  6
AG047518|AG047518.1 Pan troglodytes DNA, clone: PTB-027B01.R.     50  0.059  1
BQ787948|BQ787948.1 il45d10.x1 HR85 islet Homo sapiens cDNA clone IMAGE:6033066 3', mRNA sequence.    50  0.059  1
AL932742|AL932742.1 Anopheles gambiae EST, clone NAP1-P99-A-02-5.    50  0.059  1
AC115118|AC115118.3 Mus musculus chromosome UNK clone RP23-84N24, WORKING DRAFT SEQUENCE, 2 unordered pieces.    40  0.11  6
AC108951|AC108951.4 Mus musculus clone RP24-136D23, complete sequence.    40  0.15  4
AC144517|AC144517.7 Medicago truncatula clone mth2-13k17, complete sequence.    40  0.23  4
AL157716|AL157716.17 Human DNA sequence from clone RP5-1045M14 on chromosome 1.    50  0.42  2
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR382132_329(CR382132|pid:none) Yarrowia lipolytica strain CLIB1...   152   1e-35
FN392322_263(FN392322|pid:none) Pichia pastoris GS115 chromosome...   151   3e-35
CU928176_439(CU928176|pid:none) Zygosaccharomyces rouxii strain ...   145   3e-33
(P49018) RecName: Full=GPI-anchor transamidase;          Short=G...   143   1e-32
AE016817_700(AE016817|pid:none) Ashbya gossypii (= Eremothecium ...   142   3e-32
CR382138_1225(CR382138|pid:none) Debaryomyces hansenii chromosom...   140   8e-32
CR382138_1120(CR382138|pid:none) Debaryomyces hansenii strain CB...   140   8e-32
FM992688_685(FM992688|pid:none) Candida dubliniensis CD36 chromo...   139   2e-31
CP000500_66(CP000500|pid:none) Pichia stipitis CBS 6054 chromoso...   137   5e-31
AK117596_1(AK117596|pid:none) Arabidopsis thaliana At1g08750 mRN...   136   1e-30
protein update 2009. 6.17
PSORT 

 psg: 0.95  gvh: 0.58  alm: 0.24  top: 0.00  tms: 0.13  mit: 0.37  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 24.0 %: mitochondrial
	 12.0 %: plasma membrane
	  8.0 %: cytoplasmic
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFB893 is end
5' end seq. ID VFB893F
5' end seq. 
>VFB893F.Seq
CACTGTTGGCCTACTGGNATTTTTTAATTTAATTTTAATTTATTTTTATTTATATATATA
CACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATTTTATCTTGTATTT
TTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAATTTCAAATGGTGGG
GCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAATGGTAAAGCGGAT
TATAATGTNGAACATTTTTTCAGAGGTGAACATACAAATAATNGGGCACTTTTAGTTTGC
ACATCAAGANTNTGGTTTAACTATAGACATATAGCAA----------
Length of 5' end seq. 337
3' end seq. ID VFB893Z
3' end seq. 
>VFB893Z.Seq
----------AAGATATCATGAAATTTTATTCATGGTTGATACTTGTCAAGCTAATACAT
TATATACTCGTTTTAATTCTCCAAATATTTTAGCAATTGGTTCAAGTAAATTTGGTGAAA
ATAGTTATTCTCATCATTCAGATCCAGAATTAGGGGTTGCAGTTATTGATAGATTTACAT
ATTATACATTGGAATTCTTTGAAAGTGTTGACCCACATAATGTAACATTATATCAATTGT
TCAATACATATAGTCCACAAAAATTACAATCACATAGTGAATATAGGACAGATTTATTCA
ATAGACCATTAGATCAAGTTCCTGTTACAGACTTTTTTGGTTCTGTAATGAGAGCTGATT
TAACTCCTTCAACAATTGTAAATGAAAAACAAAAAGATGAAAAAGTAGAAAAAGATGAAA
CTACCGTAAATAATAATAATAATAATAATAACAACGATAATGTAAAAAAACAAACACCAA
AAACTAAACAAATTTCATATAGAATTACAGAGAAACCAACATCAATCCAAGTACAAAATA
ACAAAGATAGTGAATTAAATCTTTTCAATTCAAAATTTATTACTTCAGCTTTAATATTTT
TAGTAGTATTTGGATTACTTTCTTGGTCTTCTGATTTTGTAAAATCAAATCTTATTTTAA
ATACAAATNATTTAAAAGATCTGAAATCAAAATCAAAGCAAATTTAAGTAATATATAAAT
AAATAAATAATAAATAA
Length of 3' end seq. 727
Connected seq. ID VFB893P
Connected seq. 
>VFB893P.Seq
CACTGTTGGCCTACTGGNATTTTTTAATTTAATTTTAATTTATTTTTATTTATATATATA
CACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATTTTATCTTGTATTT
TTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAATTTCAAATGGTGGG
GCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAATGGTAAAGCGGAT
TATAATGTNGAACATTTTTTCAGAGGTGAACATACAAATAATNGGGCACTTTTAGTTTGC
ACATCAAGANTNTGGTTTAACTATAGACATATAGCAA----------AAGATATCATGAA
ATTTTATTCATGGTTGATACTTGTCAAGCTAATACATTATATACTCGTTTTAATTCTCCA
AATATTTTAGCAATTGGTTCAAGTAAATTTGGTGAAAATAGTTATTCTCATCATTCAGAT
CCAGAATTAGGGGTTGCAGTTATTGATAGATTTACATATTATACATTGGAATTCTTTGAA
AGTGTTGACCCACATAATGTAACATTATATCAATTGTTCAATACATATAGTCCACAAAAA
TTACAATCACATAGTGAATATAGGACAGATTTATTCAATAGACCATTAGATCAAGTTCCT
GTTACAGACTTTTTTGGTTCTGTAATGAGAGCTGATTTAACTCCTTCAACAATTGTAAAT
GAAAAACAAAAAGATGAAAAAGTAGAAAAAGATGAAACTACCGTAAATAATAATAATAAT
AATAATAACAACGATAATGTAAAAAAACAAACACCAAAAACTAAACAAATTTCATATAGA
ATTACAGAGAAACCAACATCAATCCAAGTACAAAATAACAAAGATAGTGAATTAAATCTT
TTCAATTCAAAATTTATTACTTCAGCTTTAATATTTTTAGTAGTATTTGGATTACTTTCT
TGGTCTTCTGATTTTGTAAAATCAAATCTTATTTTAAATACAAATNATTTAAAAGATCTG
AAATCAAAATCAAAGCAAATTTAAGTAATATATAAATAAATAAATAATAAATAA
Length of connected seq. 1064
Full length Seq ID -
Full length Seq. -
Length of full length seq. -